{"title":"Isolation and molecular phylogeny of RFT1 gene from upland rice leaves cultivar","authors":"Sulaiman Mohammed","doi":"10.38058/ijsl.701095","DOIUrl":"https://doi.org/10.38058/ijsl.701095","url":null,"abstract":"Rice is a modern short-day plant but equally flower during non-inductive long-day condition due to the possession of second florigen, Rice Flowering Locus T1 (RFT1) . RFT1 is an ortholog of Arabidopsis FT gene which triggers flowering induction under long-day (LD) condition. The gene isolation from Malaysian upland rice cv. Wai that induces flowering were reported. Nucleic acids isolation and amplification of the RFT1 were observed, then phylogenetic relationship among other 5 indica and 1 japonica rice were inferred by comparing the gene amino acids sequence data sets using multiple sequence alignment databases. The amplicon quality result indicated that the gene was fully amplified at above 0.5kb, whereas the sequence alignment revealed that the RFT1 gene was partially amplified based on the gaps identified. Among the 6 indica cultivars sequence aligned, they were almost all conserved except on only 12 amino acids. While the cladogram tree result classified the cultivars into three major groups and include Wai under clade that contains both indica and japonica cultivars. This finding concluded that the cultivars are of high evolutionary relationship and well established their molecular relationship. The cultivars relationship provides necessary information for better understanding of molecular evolution and designing scientific breeding system for generation of new rice cultivar.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116237064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ferro-chelatase enzyme activity of blue green algae from Yeşilırmak","authors":"V. Konar, Ö. İdil, E. Çelikoğlu, Umut Çelikoğlu","doi":"10.38058/ijsl.768264","DOIUrl":"https://doi.org/10.38058/ijsl.768264","url":null,"abstract":"Blue green algae are microscopic photosynthetic bacteria, naturally in ponds, rivers, lakes and streams. Tetra pyrroles can be classified based on the presence, position and substituents of a chelated metal in the pyrrole ring. Heme and chlorophyll, which are the most common tetra pyrolle in nature, are synthesized by blue green algae. Heme is an essential cofactor for virtually all forms of life and the last step of heme biosynthesis is catalyzed by ferrochelatase enzyme. In this study, blue green algae, photosynthetic bacteria, isolated from Yesilirmak were used. Eight morphologically different isolates were obtained. The highest specific activity belongs to isolate 8 as 0.217 Umg -1 .","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115027987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coatomic refinable modules","authors":"B. Türkmen, E. Türkmen","doi":"10.38058/ijsl.774962","DOIUrl":"https://doi.org/10.38058/ijsl.774962","url":null,"abstract":"In this article, we define the concept of (strongly) coatomic refinable modules as a proper generalization of (strongly) refinable modules. It is shown that: (1) every direct summand of a coatomic refinable module is coatomic refinable; (2) over a left max ring a module M is (strongly) coatomic refinable if and only if it is (strongly) refinable; (3) if a coatomic refinable module M is π-projective, then it is strongly coatomic refinable; (4) if coatomic direct summands lift modulo every coatomic submodule of M , then M is coatomic refinable.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129674855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An easy and reliable method for establishment and maintenance of tissue cultures of Nicotiana tabacum cv TAPM 26","authors":"Y. Kaya, F. Huyop","doi":"10.38058/ijsl.764947","DOIUrl":"https://doi.org/10.38058/ijsl.764947","url":null,"abstract":"In vitro regeneration of Nicotiana tabacum was efficiently achieved using growth regulators combination supplemented into semi-solid MS medium using leaves from 1-month-old plant as explants. The growth regulators used were NAA for auxin (0.0, 1.0, 2.0, and 3.0 mgL -1 ) and BAP (0.0, 0.5, 1.0, 2.5, and 5.0 mgL -1 ). The effects of growth regulator combination were assessed based on a number of callus formation, shoots formation and fresh callus weight. The maximum number of callus formation was 100 % at five hormone combination, observed at the fourth week after culture. Maximum number of shoots produced per explant was 21.4 shoots at 1.0 mgL -1 BAP + 3.0 mgL -1 NAA, after 6 weeks of culture. The maximum callus fresh weights were obtained at 0.5 mgL -1 BAP + 1.0 mgL -1 NAA after 6 weeks (9.92 g). The best combination for shoots regeneration of Nicotiana tabacum was 1.0 mgL -1 BAP + 3.0 mgL -1 NAA. And the best combination for heaviest callus production was 0.5 mgL -1 BAP + 1.0 mgL -1 NAA.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116395597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effects of concentration based on the absorbance form the ultraviolet–visible (UV-VIS) spectroscopy analysis","authors":"Jamal Moammar Aldabib, M. Edbeib","doi":"10.38058/IJSL.633876","DOIUrl":"https://doi.org/10.38058/IJSL.633876","url":null,"abstract":"This experiment utilizes the material characterization technique known as UV-Vis spectroscopy. The aim of this research is to study the UV-Vis absorption spectroscopy readout in order to investigate the effect of concentration on absorbance for gold solution and to determine the value of band gap for powder sample (TiO 2 ). The types of TiO 2 specimens can be characterized on a solid form of powder, thin film and/or in a liquid form. The preparation of each state of specimen are varies. In this current research, Liquid samples of Au nanoparticles dispersed in different concentrations of ethanol were characterized using UV-Vis technique. The absorbance versus concentration plot of the Au-ethanol samples were found to obey Beer’s law. When examining the solid state in a form of TiO 2 powder, the sample need to be packed before running the experiment. To calculate the band gap of each matter from the reflectance data of UV-Vis, the graph of energy (eV) versus is plotted. The J-curve is identified quickly, extrapolating the tangent to intersect the x-axis to obtain the band gap value.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124915659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Sperm Storage and Artificial Insemination in Honey Bees","authors":"A. Özkök, Murat E. Selçuk","doi":"10.38058/IJSL.661629","DOIUrl":"https://doi.org/10.38058/IJSL.661629","url":null,"abstract":"To obtain the expected yield from a honeybee colony, queen bee is required to have high egg capacity, the colony is also expected to be resistant to diseases, to have a low tendency for swarming, to be resistant to looting, and to be able to adapt to the climatic conditions of the region. In this context, it is important to protect the pure bee races and to improve them by conducting breeding research. To maintain the high yield aspect of honeybee colony, the queen bee needs to be replaced at most every 2 years. Queen bee becomes adult in as short as 16 days after hatching, and mating is realized in the air outside the colony, which makes it difficult to preserve the genetic line of the queen bee. At this point, artificial insemination and protection of gene resources become important. Honey bee (Apis mellifera L.) sperm can be stored for short and long periods. However, due to the delicate structure and biology of honey bee sperm, the high success rate in mammals could not be achieved in storing bee sperm. Due to the genetic damage exposed during the freezing of honey bee sperm, long-term storage difficulties are experienced. In addition, the concentration, motility and viability of spermatozoa decrease due to the short-term storage and storage conditions. In the breeding studies in the same region, after a period of time, gene resources decrease, and accordingly, the risk of inbreeding occurs. Instead of replacing the bee colonies that are at risk of inbreeding, a new different genome addition to the colony whose current yield characteristics are known can be made through the sperm storage of other colonies. Thanks to the long-term storage of sperm, long-period genetic studies can be carried out as in mammals, which is important for improving yield characteristics genetically. In addition, the long-term storage of honeybee sperm is a hope for the protection of regional races that are in danger of extinction due to unconsciousness and improper breeding policies.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125694722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Agriculture History and Policy","authors":"Abdulgani Devlet","doi":"10.38058/IJSL.685412","DOIUrl":"https://doi.org/10.38058/IJSL.685412","url":null,"abstract":"The ancient Fertile Crescent in particular, is commonly comprehended as the origin of agriculture. The location of Western Asia covers the area of Mesopotamia and the Levant, and is limited by Syrian Desert to the south and the Anatolian Plateau to the north. First of all, a good diagnosis is needed to treat the disease, it is necessary to make a good diagnosis and definition for our sectors such as agriculture which connects our past, current and future life. In the agricultural sector, like other sectors, if we ask for a timely diagnosis and treatment of issues; certainly, we don't have to demand more because of neglect. The basis of the agricultural sector also is a science. World population has been growing and natural resources such as land and water is already under stress. To make a nation healthy and to meet food demand along with nutrition security, agriculture would continue remain as the top most priority sector for any country. Agriculture provides food, fibre and raw materials to industry. It contributes significantly to Gross Domestic Product (GDP). Agriculture provides cleaner, better environment for people to lead healthy life on earth. Moreover, it provides employment to large section of the people. If a nation has to be strong, then it has to be self-reliant coupled with strong agricultural economy that supports ecological and nutritional security. The history of agriculture has showed an important value in human development. More than half of all human around the World once efforted in farming, and even today, few—if any—humans could survive without it.This paper depicts the agricultural history and policy reforms and structural changes in World from past to the current times.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"153 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116871595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seda Mesci, S. Marakli, Burak Yazgan, Tuba Yıldırım
{"title":"The effect of ATP-binding cassette (ABC) transporters in human cancers","authors":"Seda Mesci, S. Marakli, Burak Yazgan, Tuba Yıldırım","doi":"10.38058/IJSL.594000","DOIUrl":"https://doi.org/10.38058/IJSL.594000","url":null,"abstract":"Multi-drug resistance (MDR) can be defined as a negative effect on the drug-target relationships, decreasing in intracellular drug aggregation. ABC (ATP-binding cassette) are popular transporter proteins in the development of MDR. ABC proteins in the cell membrane structure reduce the therapeutic efficacy and cause drug resistance in cancer patients. Resistance to chemotherapy is a remarkable and important issue in the treatment of cancer patients. These drug resistance proteins interfere with the drugs administered to patients, resulting in a decrease in bioavailability of these drugs and a reduction in their passage through the blood-brain barrier. ABC transporters are the largest family of transmembrane proteins with 8 subfamilies assigned from A to G based on sequence and structural homology. In this review, general information about ABC proteins is mentioned and the role of ABC proteins causing MDR in cancers is also discussed.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124795113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physicochemical properties of both drinking and domestic waters in Çorum","authors":"Emre Bezgin, Cem Ozan Eryücel, Z. Tuncel","doi":"10.38058/IJSL.594005","DOIUrl":"https://doi.org/10.38058/IJSL.594005","url":null,"abstract":"Water is the most basic need of life since the existence of living things. Physicochemical monitoring of water quality is important for public health.In this study, we aimed to research the quality of drinking and domestic waters in dams, sources, wells and water tanks,located in Corum in terms of several physicochemical parameters. In winter, spring and summer seasons we collected a total of 2894 water samples from 3 dams, 10 well-sources, and water tanks on a monthly basis; water tanks in the 80 different locations in the Center six days a week,and lastly domestic waters five days a week in 250 ml sterile water sampling bottles.We used the nephelometric method for determining the amount of turbidity, the electrometric method for analyzing conductivity, pH and, dissolved oxygen the photometric method for measuring free chlorine, the spectrophotometric method for determining the amount of sulphate, iron, nitrite, ammonia and, manganese and lastly TS 266 volumetric titration for analyzing total alkalinity, organic matter, magnesium, calcium and total hardness. We monitored water height, filling rate, turbidity, pH and free chlorine control in each drinking water storage tanks in Corum with the Scada System and free chlorine with both the Scada System and manually. Detected in the range of Ammonium and Iron (mg/L): <0.5; Nitrite (mg/L): <0.02; Manganese (mg/L): <0.01; Turbidity (NTU): between 0.1 and 0.4; Conductivity (μS / cm): between 400 and 600; pH: 7.5 to 8.0; Sulfate (mg/L): 10 to 45; Total Alkalinity (mg/L): between 180 and 250; Organic matter (mg/L): 1 to 3. In accordance with Turkish Regulation on Water Intended for Human Consumption, the several physicochemical parameters in drinking and domestic waters in Corum are in between stated values.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"371 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134144632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Retrotransposon Analyses in Cucurbitaceae family","authors":"S. Marakli","doi":"10.38058/IJSL.592537","DOIUrl":"https://doi.org/10.38058/IJSL.592537","url":null,"abstract":"Retrotransposons are class I mobile elements, moving via an RNA intermediate. There are many retrotransposon-based molecular markers. Here, Nikita and Sukkula retrotransposons were investigated in Cucumis sativus L., Citrullus lanatus and Cucumis melo L. by using IRAP (Inter-Retrotransposon Amplified Polymorphism) molecular marker method. These barley-specific retrotransposons were identified in three plant species for the first time. Moreover, band profiles of the retrotransposons were similar in plants, indicating h omomorphic band profiles. Findings could provide valuable information for understanding genomes of these plants and evolutionary relationships among them.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"191 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122058644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}