International Journal of Science Letters最新文献_第5页

Photophysical Chracterization of Galanthus elwesii Hook 加兰花的光物理特性研究

International Journal of Science Letters Pub Date : 2019-08-19 DOI: 10.38058/IJSL.591261

E. Ay, Melek Gül, B. Kocaman, M. Açıkgöz

引用次数: 0

Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates 原料乳和奶酪源凝固酶阳性分离物中金黄色葡萄球菌和葡萄球菌肠毒素的检测

International Journal of Science Letters Pub Date : 2019-08-19 DOI: 10.38058/IJSL.596007

Tuba Yıldırım, Farid Sadati, Berna Kocaman, B. Sırıken

{"title":"Staphylococcus aureus and Staphylococcal enterotoxin detection in raw milk and cheese origin coagulase positive isolates","authors":"Tuba Yıldırım, Farid Sadati, Berna Kocaman, B. Sırıken","doi":"10.38058/IJSL.596007","DOIUrl":"https://doi.org/10.38058/IJSL.596007","url":null,"abstract":"In the present study, a total 110 (60 cheese and 50 cows’ raw milk) samples was analyzed for CPS according to Food Drug Administration (FDA, 2001) previously. After the isolation, to confirmation of the isolates, catalase test, microscopic examination, coagulase test in tube and glucose-mannitol fermentation tests were applied. After the tests, we obtained 97 CPS isolates. The 97 CPS isolates were used as a material. For the confirmation of the isolates being Staphylococci species, 16S rRNA was detected by using PCR assay. For the detection of the CPS isolates being S. aureus , nuc gene detected in the CPS isolates using PCR assay. To determination of the staphylococcal enterotoxin, PCR assay was applied. The 16 S rRNA was detected in a total 97 (35 milk origin and 62 cheese origin) isolates. Therefore, these isolates were evaluated as coagulase-positive staphylococci ( CPS). The nuc gene was detected in 50 out of 97 CPS isolates. So, the 50 (18 cheese origin and 32 milk origin) isolates were evaluated as S. aureus. However, none of the staphylococcal enterotoxin genes (SE A,B,C,D,E,G,H,I,J) was detected in 97 CPS or 50 S. aureus isolates.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117014404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Microbiological properties of both drinking and domestic waters in Çorum Çorum饮用水和生活用水的微生物特性

International Journal of Science Letters Pub Date : 2019-08-19 DOI: 10.38058/IJSL.594002

Seda Mesci, Z. Tuncel, Rüveyda Turbay

{"title":"Microbiological properties of both drinking and domestic waters in Çorum","authors":"Seda Mesci, Z. Tuncel, Rüveyda Turbay","doi":"10.38058/IJSL.594002","DOIUrl":"https://doi.org/10.38058/IJSL.594002","url":null,"abstract":"Drinking water is of vital importance for all living things and regular monitoring of water quality is of great importance for public health.In this study, we aimed to examine some microbiological characteristics and to be evaluated in terms of the health of drinking water and wells used in Corum. In Corum province, once a month from ten sources-wells and water tanks used for consumption and five days a week from the central stopcock water relating to 40 locations; samples were taken in observance with the hygiene precept to 250 ml private bacteriological sterile drinking water cruet with 10% sodium thiosulfate solution. In winter, spring and summer seasons, total 1234 samples taken from sources-well, water tanks and central city tap water between January and July were used. A total of 66 samples taken from resources and wells used for consumption in Corum proved to have seasonal changes, and some increment and diminish in bacteria rate are defined. According to the “Regulation on Waters for Human Consumption”; Chloride value: 0.2-0.5 mg/L (photometric method) was detected in the value range, and at the end daily control and checking, 1168 samples were gathered from the water samples among January and July in Corum province. As a result of the information determined, there is no negative and contradictory data.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117345398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transposons Continue the Amaze 转座子继续奇迹

International Journal of Science Letters Pub Date : 2019-08-19 DOI: 10.38058/IJSL.585052

N. Gozukirmizi

{"title":"Transposons Continue the Amaze","authors":"N. Gozukirmizi","doi":"10.38058/IJSL.585052","DOIUrl":"https://doi.org/10.38058/IJSL.585052","url":null,"abstract":"Transposable elements ( TEs ) were first discovered in maize plants. However, they exist almost in all species with a few exceptions ( Plasmodium falciparum, Ashbya gossypii and Kluveromuyces lactis ) . They are the most important contributors to genome plasticity and evolution and even epigenetic genome regulation. Organisms with large genomes have high transposon percentages. For example, Arabidopsis thaliana has a genome size of 125 Mb which comprises 14% transposons, Homo sapiens (3000 Mb) 45-48.5%, and Hordeum vulgare genome (5300 Mb) has 80%. TEs are classified into two major groups based on their transposition mechanisms: Class I (RNA transposons – retrotransposons) and Class II (DNA transposons). Recent progress in whole-genome sequencing and long-read assembly have resulted in identification of unprecedentedly long transposable units spanning dozens or even hundreds of kilobases, initially in prokaryotic and more recently in eukaryotic systems. All TEs in a cell are named as transposome (mobilome), and transposomics is a new area to work with transposome. Although a number of bioinformatics softwares have recently been developed for the annotation of TEs in sequenced genomes, there are very few computational tools strictly dedicated to the identification of active TEs using genome-wide approaches. In this review article, after a brief introduction and review of the transposable elements, I discussed their effects in gene expression, evolution, recent applications and also share our research on retrotransposons with different organisms.","PeriodicalId":287513,"journal":{"name":"International Journal of Science Letters","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117337901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1