S. Silva, A. Batista, J. Domingues, M. Quadrado, M. Morgado
{"title":"Fluorescence lifetime microscope for corneal metabolic imaging","authors":"S. Silva, A. Batista, J. Domingues, M. Quadrado, M. Morgado","doi":"10.1109/ENBENG.2015.7088878","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088878","url":null,"abstract":"Assessing corneal metabolism may provide clinicians a tool for diagnosing corneal cells dysfunctions prior to its pathological expression. Flavin adenine dinucleotide (FAD), a metabolic co-factor, exhibits two lifetime components (long and short) upon blue light excitation. Due to that, fluorescence lifetime imaging microscopy (FLIM) may provide a method to evaluate corneal cells metabolism non-invasively. We are developing a single-photon, time-gated fluorescence lifetime microscope for in vivo corneal imaging using structured illumination to improve optical sectioning. Single-photon imaging is provided by a picosecond diode laser with emission at 443nm. Structured illumination is implemented by modulating the laser light through a Digital Micromirror Device (DMD). The fluorescence imaging acquisition is based on an ultrafast time-gated intensified CCD camera operating with gates down to 200ps. We present preliminary data regarding the timing and optical performance of the microscope.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125240549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Pereira, R. Sanches, P. Reis, J. Pêgo, Ricardo Simões
{"title":"Correlation study between blood pressure and pulse transit time","authors":"T. Pereira, R. Sanches, P. Reis, J. Pêgo, Ricardo Simões","doi":"10.1109/ENBENG.2015.7088864","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088864","url":null,"abstract":"Blood pressure (BP) determination is a fundamental parameter in cardiovascular assessment. The gold standard method to measure BP is based on the inflatable arm cuff, however has several disadvantages for continuous monitoring. New techniques were developed to overcome these limitations using correlations between the pulse transit time (PTT) and BP. This work draws attention to the PTT rationale using several methods. In order to determine the PTT, an electrocardiogram (ECG) was used combined with multiple photoplethysmography (PPG) sensors applied to different arm locations, these signals were acquired with a bioPLUX device. The Ultrassound system (SonoSite Edge) was used to measure the artery diameter. As reference, BP was measured using a cuff-based sphygmomanometric device. Measurements were performed in a study population of 36 volunteers. The correlation coefficient for DBP determined and DBP measured was r = 0,689. The results suggest PTT deduced from different locations can be used to measure BP.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"23 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123730096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Computer-aided bone fracture identification based on ultrasound images","authors":"Luís Nascimento, M. Graca Ruano","doi":"10.1109/ENBENG.2015.7088892","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088892","url":null,"abstract":"Clinical diagnosis is increasingly being supported by complementary imaging methods, many of them based on ionizing radiation such as X-ray. Bone fractures are traditionally diagnosed through X-ray. On the other hand ultrasound imaging represents a cheap and fast alternative, particularly when stress fractures are considered. Framed by a computer-aided system a 3-stage procedure for automatic identification of bone fractures on ultrasound images is proposed. Combined methods of speckle reduction, bone line detection and fracture identification, tuned by well performed variables, enabled correct classification of bone fractures in 89% of the 44 analyzed images. Stress fractures were all correctly identified in comparison with the clinical records therefore proving the ultrasound imaging primacy against X-ray for this type of pathology and encouraging its broader application on other type of bones.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131774867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tiago Marçal, Bruno Antunes, R. Ferreira, C. Correia, Diana Pires, A. Matos, Joao Nuno Simoes
{"title":"Wireless multi-physiological signal monitor for clinical discharge and readmissions criteria setting and ambulatory usage","authors":"Tiago Marçal, Bruno Antunes, R. Ferreira, C. Correia, Diana Pires, A. Matos, Joao Nuno Simoes","doi":"10.1109/ENBENG.2015.7088846","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088846","url":null,"abstract":"A monitoring system measuring 4 physiological variables (respiratory and cardiac frequencies, skin temperature and acceleration) has been developed and tested. Details on the electronics and software are discussed with emphasis on energetic autonomy. The general rules for selecting adequate sampling rates for each variable are discussed, considering its nature and the amount of storage data that it implies. The three levels of software that hat to be written and articulated: one that resides in the microcontroller, another one at the smartphone and a last one for data analysis, also are discussed. Some aspects of data analysis software that is meant to provide the medical staff with readily intelligible information, are also discussed. Results, collected in a small population of volunteers are shown and commented.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115083910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. J. Oliveira, S. B. Goncalves, A. C. Peixoto, A. F. Silva, J. Correia
{"title":"Long neuroprobes based on silicon dicing and iridium oxide for electrical stimulation/recording","authors":"M. J. Oliveira, S. B. Goncalves, A. C. Peixoto, A. F. Silva, J. Correia","doi":"10.1109/ENBENG.2015.7088855","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088855","url":null,"abstract":"This paper synthesizes the development of a new 3D neuroprobe array, constituted by microelectrodes capable of electrical stimulation and recording. The array's design relies on a bulk silicon substrate dicing technology and it comprises 6 × 6 neuroprobes with three different penetrating lengths (from 3 to 4 mm) and a 180 μm cross-section. A reproducible fabrication method was demonstrated, capable of producing longer shafts than in previously reported arrays. In addition, sputtered titanium/iridium oxide microelectrodes have shown the required performance, with a consistent reversible electrochemical behavior and an impedance value of 145 Ω in the same frequency range as the stimulation protocols.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132235445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Areias, M. Parente, F. Gentil, R. N. Natal Jorge, A. Fernandes
{"title":"Biomechanical simulation of the human ear","authors":"B. Areias, M. Parente, F. Gentil, R. N. Natal Jorge, A. Fernandes","doi":"10.1109/ENBENG.2015.7088813","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088813","url":null,"abstract":"Summary form only given. In this study, the biomechanical behaviour of the human hearing was studied. An accurate finite element model can be used to evaluate the influence of certain hearing diseases and aid in their treatment, as well as help to comprehend the phenomenon of sound transmission. This research was based on the project \"The Visible Ear\" developed by a group of Danish students in order to obtain a set of high quality images of the temporal bone, using a \"cryosectioning\" procedure [1]. This project allowed the development of the computational models used. CAD software was used to treat each of the parts that compose the human ear. The first tested model (simple model) was composed of the tympanic membrane, ossicles, cochlea, two muscles, six ligaments, two joints, and the temporal bone. The full model was based on the simple model adding air to the external auditory canal and middle ear, the skin, the jaw and the ear cartilage. Comparing the results obtained by both models, it was possible to understand the importance of the ear canal in the transmission of the sound to the eardrum. The models were simulated numerically with the Abaqus software. The natural frequencies, displacements of the umbo and stapes footplate were obtained for both models, for different sound pressure levels. The frequency band used in this study was between 100 Hz and 10 kHz.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122367871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mafalda Carvalho, Rui Garces, C. Tavares, I. Rocha, T. Russomano
{"title":"Wavelet analysis of heart rate variability during microgravity simulation","authors":"Mafalda Carvalho, Rui Garces, C. Tavares, I. Rocha, T. Russomano","doi":"10.1109/ENBENG.2015.7088860","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088860","url":null,"abstract":"Lately, heart rate variability (HRV) and blood pressure adaptations have been studied in relation to Space Exploration. Most data obtained so far, indicate a decrease in heart rate (HR), mainly explained by a decrease in the low frequency component (LF) of HRV and an increase in the high frequency band (HF), indicating a predominant vagal modulation during space flights. In order to validate our laboratory and in-house analysis software, as potential tools for space life sciences, 14 normal subjects participated in a 2 hours protocol of simulated weightlessness (HDT-6o) in addition to a Mental Stress Maneuver. All cardiac components were collected, and after wavelets analysis, preliminary results show a significant decrease in HR (p=0,0128). The LF/HF ratio shows a clear tendency to decrease, with a comprehensible increase in HF. During Mental Stress, HR was increased, correlating with higher LF, alongside a general decrease in HF. Overall, our preliminary results validate our laboratory, for studies in the field of human space physiology.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"252 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128808248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nataniel Gonçalves-Rosa, C. Tavares, V. Geraldes, C. Nunes-Da-Silva, I. Rocha
{"title":"Application of a modified Hilbert-Huang Transform to autonomic evaluation in metabolic syndrome","authors":"Nataniel Gonçalves-Rosa, C. Tavares, V. Geraldes, C. Nunes-Da-Silva, I. Rocha","doi":"10.1109/ENBENG.2015.7088858","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088858","url":null,"abstract":"The physiological signals are a challenge in terms of identification and processing due to its lack of stationarity and linearity. The autonomic output to the cardiovascular system is classically quantified by analysis of heart rate variability. The Fast Fourier Transform (FFT) and Discrete Wavelet Transform applied to the cardiac signals showed limitations in terms of time and frequency resolutions. With this work we propose the application of a Modified Hilbert-Huang Transform for tachogram and systogram evaluation with the introduction of a new method to smooth fluctuations endpoint. This work presents a comparative study using FFT and Hilbert-Huang methodologies. It is known that patients and animal models with diseases involving sympathoexcitation, eg.: Metabolic Syndrome (MetS), show increases in low frequencies band. Therefore, these mathematical tools are evaluated by analyzing experimental data from two animal species: MetS-rabbits (n=6) and MetS-rats (n=6) and their respective lean age and sex-matched controls (n=6 for each group). Modified Hilbert-Huang Transform analysis showed a sympathoexcitatory condition in MetS comparing to controls both in a rabbit and in a rat model of MetS. These significant differences on sympathetic tone were also confirmed by FFT but, the parasympathetic activity, was similar between normal and MetS animals. Results and its physiological interpretation clearly show the suitability of the modified Hilbert-Huang Transform as an alternative methodology for the dynamic assessment of the autonomic nervous system in MetS.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"238 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123281368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Urinary incontinence in musicians: A preliminary study","authors":"M. Masteling, T. Mascarenhas, R. Natal","doi":"10.1109/ENBENG.2015.7088838","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088838","url":null,"abstract":"There has been a graduate interest in the long-term health risks associated with music practice at a professional and semi-professional level. Playing a wind instrument resembles the effects of physical exercise and is compared to the ones of a Valsalva Maneuver (VM). Strenuous exercise due to the increase of the Intrabdominal Pressure (IAP) may be considered a risk factor for urinary incontinence (UI) (3). Being established that increases in IAP are related with a higher risk of UI in young female and playing a wind instrument leads to an increase IAP, the aim of this work is to analyze the impact that playing a wind instrument can have in women, based in the different internal pressures they are subjected to due to their practice. To test this hypothesis, questionnaires will be performed by female musicians with different music backgrounds.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"219 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116865217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of investment casting of Ti6Al4V HIP prostheses by numerical and experimental methods","authors":"N. Song, Shenghua Wu, R. Neto, M. Machado","doi":"10.1109/ENBENG.2015.7088830","DOIUrl":"https://doi.org/10.1109/ENBENG.2015.7088830","url":null,"abstract":"Hip prosthesis presents a cylindrical shape with high length to diameter ratio. This medical device has to withstand many years of cyclical loading equal to at least 3 to 5 times the body weight. This intensive use requires that the product must have very good performance [1]. Ti6Al4V is a biocompatible alloy that is widely applied for manufacturing hard-tissue replacement systems such as total hip prostheses. Investment casting as a near net shape forming method is a costless and good way to produce this type of orthopaedic implants. Nonetheless, this casting process is not a straight forward task and it will easily cause centre line shrinkage. Also, Ti6Al4V is a highly reactive alloy in molten state, which will react with the ceramic shells used in investment casting. Casting defects like shrinkage and residual stress may lead to the premature fatigue failure of the prosthesis as this device is subjected to approximately one million of cyclic loads per year. Thus, during the investment casting of Ti6Al4V it is crucial to develop methodologies for eliminate or minimize the surface oxidization and central line shrinkage [2]. The face coat material has a significant effect on the surface property of this kind alloy. Moreover, the wrap technique influences the solidification sequence of the alloy. In this study, an optimization method for the investment casting of hip prostheses was explored. Different face coat materials of shell were also investigated throughout this work. Furthermore, the solidification sequence of this long cylinder shaped part was studied. Herein, experimental and numerical methods were applied. The experimental results matched well with the outcomes of the simulation runs, which validates and corroborates the proposed optimization methodology.","PeriodicalId":285567,"journal":{"name":"2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG)","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121502357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}