{"title":"Comparison of heparin-immobilized vs. antibody-immobilized microspheres for the capture and detection of cytokines during microdialysis sampling","authors":"J. Duo, Randy F. Espinal Cabrera, J. Stenken","doi":"10.1109/LISSA.2009.4906722","DOIUrl":"https://doi.org/10.1109/LISSA.2009.4906722","url":null,"abstract":"Heparin-immobilized beads were compared against antibody-immobilized beads for improving the collection of targeted cytokines through microdialysis sampling probes. Heparin offers an advantage over antibodies with respect to its stability and cost. The cytokines, MCP-1 and RANTES, exhibited increased flux through the probes when either heparin-immobilized or antibody-immobilized beads were included in the perfusion fluid. This suggests that heparin-immobilized beads may be a viable alternative to antibody-immobilized beads to increase the flux of select cytokines during microdialysis sampling.","PeriodicalId":285171,"journal":{"name":"2009 IEEE/NIH Life Science Systems and Applications Workshop","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123903605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engineered nanotopographic structures for applications in tissue engineering and regenerative medicine","authors":"J. Borenstein, C. Bettinger","doi":"10.1109/LISSA.2009.4906699","DOIUrl":"https://doi.org/10.1109/LISSA.2009.4906699","url":null,"abstract":"Nanotopographic structures occur naturally within the extracellular matrix of many tissues, influencing a wide range of properties through mechanotransductive interactions. Synthetic cell-nanotopography interactions have been explored as a way of controlling cell behaviors including orientation, adhesion, migration, proliferation and cytoskeletal organization. Until recently these processes have been explored using traditional cell culture substrates for laboratory investigations, including titanium, glass, ceramics, silicon, polystyrene and PolyDiMethylSiloxane (PDMS), as well as on numerous disordered nanostructured materials such as collagen. Nanopatterned PDMS exhibits unique utility for in vitro studies including fundamental studies on cell-nanotopography interactions as well as structures that can serve as template for tissue organization. Emerging research is exploring nanoscale mechanotransduction on biodegradable substrates suitable for implantation, thereby paving the way for the development of engineered tissues with tunable mechanical and functional properties. Here recent developments in nanoscale modification of substrates for tissue engineering and regenerative medicine are described, with an emphasis on how these studies might ultimately lead to advanced approaches for patient care.","PeriodicalId":285171,"journal":{"name":"2009 IEEE/NIH Life Science Systems and Applications Workshop","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131421632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuai Yuan, C. Roney, Qian Li, Michael Lai, James Y. Jiang, Biying C Xu, Hongzhou Ma, A. Cable, R. Summers, Yu Chen
{"title":"Simultaneous morphology and molecular imaging of colon cancer","authors":"Shuai Yuan, C. Roney, Qian Li, Michael Lai, James Y. Jiang, Biying C Xu, Hongzhou Ma, A. Cable, R. Summers, Yu Chen","doi":"10.1109/LISSA.2009.4906736","DOIUrl":"https://doi.org/10.1109/LISSA.2009.4906736","url":null,"abstract":"The objective of this study is to investigate the feasibility of imaging colon cancers using multi-modal optical imaging system combining optical coherence tomography (OCT) and fluorescence molecular imaging (FMI). This system enables simultaneous imaging of tissue morphology and molecular information at high resolution (10–20 μm) over a 3–5 mm field-of-view. OCT reveals the microstructures of normal colon and colon polyps, and the information is confirmed by the corresponding histology. FMI shows uptake of glycoprotein targeting contrast agents. The locations of high fluorescence intensity correspond with the characteristic morphological features of polyps revealed by the co-registered OCT imaging. Our results suggest that multi-modal optical imaging with OCT and fluorescence imaging holds strong potentials for molecular imaging and in situ characterization of colon cancers.","PeriodicalId":285171,"journal":{"name":"2009 IEEE/NIH Life Science Systems and Applications Workshop","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133093134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Plug-and-play sensor node for body area networks","authors":"A. Saeed, M. Faezipour, M. Nourani, L. Tamil","doi":"10.1109/LISSA.2009.4906720","DOIUrl":"https://doi.org/10.1109/LISSA.2009.4906720","url":null,"abstract":"Advancements in low power wireless communication and system on chip design have opened up the possibility of developing miniaturized sensor nodes. These sensors can either be deployed on the human body (non-invasive) or implanted inside it (invasive) to collect vital physiological information and wirelessly transmit it to the system database. At the system backend this data is stored, processed, analyzed, and taken action if required. In this paper we propose the hardware architecture of a low-power, low-cost, small footprint, plug & play sensor node that is suitable for monitoring the vital signs. An event driven operating system is built for the sensor node to efficiently run the house keeping tasks and communicate with the gateway. As a proof of concept, an Electrocardiogram (ECG) monitoring application for body area networks using this sensor node has been developed.","PeriodicalId":285171,"journal":{"name":"2009 IEEE/NIH Life Science Systems and Applications Workshop","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128691865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}