Journal of investigative surgery : the official journal of the Academy of Surgical Research最新文献

{"title":"Use of Prostate Systematic and Targeted Biopsy on the Basis of Bi-Parametric Magnetic Resonance Imaging in Biopsy-Naïve Patients.","authors":"Yifei Cheng,&nbsp;Feng Qi,&nbsp;Linghui Liang,&nbsp;Lei Zhang,&nbsp;Dongliang Cao,&nbsp;Lixin Hua,&nbsp;Gong Cheng","doi":"10.1080/08941939.2020.1825884","DOIUrl":"https://doi.org/10.1080/08941939.2020.1825884","url":null,"abstract":"<p><strong>Objectives: </strong>To explore the performance of targeted biopsy (TB) in combination with systematic biopsy (SB) in the detection of prostate cancer (PCa) in biopsy naïve patients.</p><p><strong>Methods: </strong>From May 2018 to January 2020, 230 biopsy-naïve men with suspicious bi-parametric MRI [bpMRI; Prostate Imaging Reporting and Data System (PI-RADS) score ≥3] were enrolled. All patients had prostate-specific antigen (PSA) levels of 20 ng/ml or less. For each patient, transrectal ultrasound-guided prostate biopsy was performed. The primary endpoint was the detection rate of CSPC [clinically-significant PCa, International Society of Urological Pathology grade group (ISUP GG) 2 or higher tumors]. The secondary endpoints were the detection rates of CIPC (clinically insignificant PCa, ISUP GG 1 tumors).</p><p><strong>Results: </strong>CSPC was detected in 90 patients. Twelve (13.33%) of them were detected by TB only and 18 (20.00%) by SB only. Detection of CSPC by SB and TB did not differ significantly (<i>p</i> = .36). In 4.35% of 230 patients, CSPC would have been missed if we performed SB only, and in 6.09% of patients if we performed TB only. Moreover, combination of TB and SB did not increase the detection of CIPC.</p><p><strong>Conclusions: </strong>No significant difference was found in the detection of CSPC between TB and SB; however, both techniques revealed substantial added value and combination of TB and SB could further improve this detection rate without increasing the detection of CIPC.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"92-97"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1825884","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38533927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant Treatment of Ureteropelvic Junction Obstruction Complicated by Renal Calculi with Laparoscopic Pyeloplasty and Pyelolithotomy via 19.5F Rigid Nephroscope: A Report of 12 Cases.","authors":"Lizhe An,&nbsp;Liulin Xiong,&nbsp;Liang Chen,&nbsp;Xiongjun Ye,&nbsp;Xiaobo Huang","doi":"10.1080/08941939.2020.1824248","DOIUrl":"https://doi.org/10.1080/08941939.2020.1824248","url":null,"abstract":"<p><p><b>Objective</b>: To introduce our experience of concomitant laparoscopic pyeloplasty (LP) and pyelolithotomy via 19.5 F rigid nephroscope to treat ureteropelvic junction obstruction (UPJO) complicated with renal calculi.<b>Methods</b>: The data of 42 patients with UPJO who underwent LP from June 2016 to August 2019 were retrospectively reviewed. Twelve patients with ipsilateral renal calculi underwent LP and concomitant pyelolithotomy via 19.5 F rigid nephroscope. Perioperative data of this group were compared with other 12 matched patients without calculi who underwent LP only.<b>Results</b>: Of 12 patients with renal calculi, only 4 patients had simple stone and the other 8 patients suffered complex stones. Anatomical solitary kidney was found in 2 patients. The mean diameter of the largest stone was 1.1 cm (ranged from 0.6 to 1.7). The mean operative time was 171 min, the mean time of nephroscopic management was 17.2 min, 7 patients received pyelolithotomy by forceps, 3 patients received pyelolithotomy and ultrasonic lithotripsy, 1 patient received ultrasonic lithotripsy, the mean number of stones retrieved was 8.9 (ranged from 0-53), in one case the calculus was inaccessible because it was located in a narrow neck caliceal diverticulum. Overall stone-free rate was 91.7% (11/12). No difference in operative time, postoperative hemoglobin drop, postoperative hospital stay and incidence of complications was observed between the 2 groups. At the mean follow-up of 17.9 months, no patients had obstruction or recurrent stones.<b>Conclusion</b>: LP and concomitant pyelolithotomy via 19.5 F rigid nephroscope is a safe and feasible option to treat UPJO with renal calculi, with acceptable success rate and stone-free rate.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"77-82"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1824248","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38358882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleeve Gastrectomy Surgery Improves Glucose Metabolism by Downregulating the Intestinal Expression of Sodium-Glucose Cotransporter-3.","authors":"Yixing Ren,&nbsp;Zhiming Zhao,&nbsp;Guodong Zhao,&nbsp;Qu Liu,&nbsp;Zizheng Wang,&nbsp;Rong Liu","doi":"10.1080/08941939.2020.1810370","DOIUrl":"https://doi.org/10.1080/08941939.2020.1810370","url":null,"abstract":"<p><strong>Aims: </strong>Sleeve gastrectomy (SG) has been proven effective in the treatment of obesity and type 2 diabetes. We hypothesized that SGLT3 may play an important role in the mechanism of glucose control and weight loss after SG.</p><p><strong>Materials and methods: </strong>Daily body weight and food intake were measured in SG and sham-operated mice. Glucose tolerance test, SGLT3 agonist (αMG), and SGLT1 inhibitor (phlorizin) perfusion experiments were used to detect changes in intestinal SGLT3 and SGLT1 activity following SG. Expression of SGLT3a and SGLT1 was assessed at 2 weeks, 1 month after surgery by quantitative PCR and fluorescence immunoassay. Hematoxylin and eosin staining was used to detect morphological changes in the villi. SGLT3 and SGLT1 expression was measured after stimulation of human intestinal epithelial cells (HIEC).</p><p><strong>Results: </strong>Both the body weight and daily food intake of the SG-treated mice decreased within 30 days after surgery. Oral glucose absorption was significantly reduced at 30 days. The intestinal stimulation proved that SG can improve glucose metabolism, which can be reversed by αMG and enhanced by phlorizin. Villus height and surface area of the intestine in SG mice decreased after surgery. mRNA expression of SGLT3a and SGLT1 decreased at 2 weeks and 1 month after SG, immunofluorescence also confirmed these changes. HIEC stimulation confirmed that αMG could increase the expression of SGLT3 and SGLT1, but the expression of SGLT1 was down regulated when phlorizin was added to the medium.</p><p><strong>Conclusion: </strong>The results suggest that reducing SGLT3 expression might contribute to lowering blood glucose and controlling body weight after SG.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"14-22"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1810370","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38301835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria Associated with Granulomatous Lobular Mastitis and the Potential for Personalized Therapy.","authors":"Xin-Qian Li,&nbsp;Hong-Li Wu,&nbsp;Jing-Ping Yuan,&nbsp;Tian-Gang Liu,&nbsp;Sheng-Rong Sun,&nbsp;Chuang Chen","doi":"10.1080/08941939.2020.1833262","DOIUrl":"https://doi.org/10.1080/08941939.2020.1833262","url":null,"abstract":"<p><p>Granulomatous lobular mastitis (GLM), also known as idiopathic granulomatous mastitis (IGM), is a chronic inflammatory lesion of the breast. The incidence of GLM has been increasing in recent years, especially among young women. The etiologies of GLM have not been fully elucidated but are associated with autoimmunity and bacterial infection. Bacteria, especially <i>Corynebacterium</i> species, play important roles in GLM. In this article, we review research progress regarding the bacteriology of GLM attained with the application of several new high-throughput detection techniques. Accurate detection might be important for deepening our understanding of the pathogenesis of GLM and hold promise for personalized GLM therapy.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"164-170"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1833262","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38490559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Acute Pancreatitis and COVID-19: Could Pancreatitis Be the Missing Piece of the Puzzle about Increased Mortality Rates?","authors":"Cevher Akarsu,&nbsp;Mehmet Karabulut,&nbsp;Husnu Aydin,&nbsp;Nuri Alper Sahbaz,&nbsp;Ahmet Cem Dural,&nbsp;Duygu Yegul,&nbsp;Kivanc Derya Peker,&nbsp;Sina Ferahman,&nbsp;Sezer Bulut,&nbsp;Turgut Dönmez,&nbsp;Sinan Asar,&nbsp;Kadriye Kart Yasar,&nbsp;Gokhan Tolga Adas","doi":"10.1080/08941939.2020.1833263","DOIUrl":"https://doi.org/10.1080/08941939.2020.1833263","url":null,"abstract":"<p><strong>Background: </strong>The COVID-19 pandemic caused by SARS-CoV-2 commenced in Wuhan China in 2019 and soon spread worldwide. SARS-CoV-2 enters the cell by binding to the ACE II receptor and begins viral replication. The effects and clinical findings of SARS-CoV-2 on the liver, kidney, heart, gastrointestinal (GI) system and especially lungs have been widely discussed. However, the effects on the pancreas-another organ that also expresses ACE II-have not been studied.</p><p><strong>Methods: </strong>This work prospectively evaluated data from 316 patients who were admitted with a diagnosis of COVID-19 pneumonia. The patients were categorized into three according to the severity of pneumonia (mild, severe, critical). Demographic data, rate of pancreatitis, biochemical parameters, and radiological images from each group were analyzed. The patients were divided into two groups and outcomes were compared: COVID-19 patients with acute pancreatitis (Group P) and without acute pancreatitis (Group C).</p><p><strong>Results: </strong>The median age was 54 (18-87), and the median age for patients with acute pancreatitis was 55 (26-84). As an expected finding, we found a positive correlation between advanced age and mortality (<i>p</i> = 0.0003). 12.6% of the patients had acute pancreatitis. While pancreatitis was not seen in patients on mild status, the rate of pancreatitis was 32.5% in critical patients. Hospitalization and mortality rates were higher in patients with COVID-19 accompanied by acute pancreatitis (<i>p</i> = 0.0038 and <i>p</i> < 0.0001, respectively). C-Reactive Protein (CRP) and ferritin were significantly higher in those who had pancreatitis (<i>p</i> < 0.0001). D-Dimer and procalcitonin levels had only a small difference (<i>p</i> = 0.1127 and <i>p</i> = 0.3403, respectively).</p><p><strong>Conclusion: </strong>Acute pancreatitis alone is a clinical condition that can lead to mortality and may be one of the reasons for the exaggerated immune response developing in the progression of COVID-19. Our results point out that the presence of pancreatic damage triggered by SARS-CoV-2 can deteriorate the clinical condition of patients and the mortality rate may increase in these patients.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"119-125"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1833263","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38558166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Efficacy of Two Laparoscopic Surgical Procedures Combined with Hysteroscopic Incision in the Treatment of Cesarean Scar Diverticulum.","authors":"Chao Peng,&nbsp;Yan Huang,&nbsp;Ye Lu,&nbsp;Yingfang Zhou","doi":"10.1080/08941939.2020.1830319","DOIUrl":"https://doi.org/10.1080/08941939.2020.1830319","url":null,"abstract":"<p><p><b>Objective</b>: To investigate the efficacy of two different surgical procedures in combined hysteroscopic and laparoscopic surgery-the \"folding suture method\" and the \"muscle flap filling suture method\"-in the treatment of cesarean scar diverticulum (CSD).<b>Methods</b>: The clinical data of 24 patients with CSD who underwent surgery in the Peking University First Hospital from August 2016 to December 2018 were retrospectively analyzed.<b>Results</b>: There was no difference in age, vaginal bleeding time, thickness of the lower uterine segment, operative time and intraoperative bleeding between the two groups. At three months after the operation, the patients of the folding suture and muscle flap groups had an average menstrual period of 6.9 ± 1.8 days and 7.5 ± 3.0 days, respectively, which was 5.8 ± 4.2 days and 4.4 ± 3.8 days, respectively, shorter than that before the operation, as well as a lower segment thickness of the uterus of 6.7 ± 1.8 mm and 6.3 ± 1.7 mm, respectively. Among the patients in the folding suture and muscle flap groups, 8 and 6 cases were cured, and 3 and 6 cases were improved, respectively, resulting in an effective rate of 100%. There was no significant difference in any indicator between the two groups.<b>Conclusion</b>: As two new surgical methods that preserve uterine integrity, the laparoscopic \"muscle flap filling suture method\" and \"folding suture method\" combined with hysteroscopic incision are safe and effective treatments for repairing CSD.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"225-230"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1830319","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38587169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sentinel Node Mapping in Endometrial Cancer.","authors":"Giorgio Bogani,&nbsp;Antonino Ditto,&nbsp;Mauro Signorelli,&nbsp;Valentina Chiappa,&nbsp;Fabio Martinelli,&nbsp;Francesco Raspagliesi","doi":"10.1080/08941939.2019.1680778","DOIUrl":"https://doi.org/10.1080/08941939.2019.1680778","url":null,"abstract":"Nodal assessment represents a crucial point in the management of endometrial cancer. Node negative uterine-confined endometrioid endometrial cancer patients experience excellent survival outcomes, being overall survival more than 80–90% at 5 years. In node positive disease, survival decreased to 60–70% at 5 years [1]. Although randomized trials failed to demonstrate a possible therapeutic role of lymphadenectomy in endometrial cancer, nodal assessment represent an integral part of staging surgery in uterine neoplasm [1]. Nodal assessment has important prognostic implication and might help to plan postoperative treatment, thus having an indirect therapeutic role [1]. Recently, sentinel node mapping replaced systematic lymphadenectomy during staging surgery for endometrial cancer [2–4]. Sentinel node mapping is associated with lower morbidity than lymphadenectomy. Moreover, sentinel node mapping is not inferior to systematic lymphadenectomy in term of detection rate. Accumulating data suggested that sentinel node mapping improves detection rate of positive nodes in comparison to lymphadenectomy, thanks to the application of ultrastaging on sentinel nodes harvested. It is estimated that the adoption of sentinel node mapping (and ultrastaging) increase positive node detection rate of more than 30%, being ultrastaging able to detect low volume disease not detectable with conventional histological examination [5]. In the present paper, the Authors investigated the role of preoperative workup in endometrial cancer patients [6]. The authors observed that PET/CT scan shows high sensitivity but moderate specificity for nodal involvement and might help in avoiding unnecessary extensive nodal dissection in patients with sentinel node mapping failure [6].Considering the growing adoption of sentinel node mapping, PET/CT might help in formulating preoperative and operative plans. However, we have to take in account that almost all scientific societies suggested to avoid the use of complex preoperative workup in endometrial cancer patients [7,8]. The American Congress of Obstetricians of Gynecologists (ACOG) recommends that “only a physical examination and a chest radiograph are required for preoperative staging of the usual (type I endometrioid grade 1) histology, clinical stage I patient” [7,8]. Similarly, the Society of Gynecology Oncology (SGO) and the European Society for Medical Oncology (ESMO), the European Society for Radiotherapy and Oncology (ESTRO), and the European Society of Gynaecological Oncology (ESGO) consensus conference do not recommend preoperative imaging assessment, suggesting the option of imaging only for patients with clinically advanced or metastatic endometrial cancer [8]. The role of adopting PET/CT and its cost-effectiveness in the context of sentinel node mapping have to be further validated into prospective trials. We can expected that costs utility of PET/CT is depending on the class of risk of endometrial cancer. As observed for ","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"677-678"},"PeriodicalIF":1.9,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2019.1680778","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37469212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Between Proteolytic Enzymes and Microangiogenesis in Degenerative Intervertebral Disc Nucleus.","authors":"Dong Chen,&nbsp;Xin Jiang","doi":"10.1080/08941939.2019.1679921","DOIUrl":"https://doi.org/10.1080/08941939.2019.1679921","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation between proteolytic enzymes and microangiogenesis in degenerative intervertebral disc nucleus. <b>Methods:</b> Forty patients with degenerative disc nucleus pulposus who were admitted to our hospital were selected incase group and 20 healthy subjects were selected into the normal group. The specimens from the case group and the control group were collected to observe the degeneration of nucleus pulposus tissues with different degrees of classification, including H&E staining and immunohistochemical staining, and observe cathepsin such as aminopeptidase and vascular endothelial positive cells. The distribution of microvessels was also performed by the Weidner method. <b>Results:</b> After H&E staining, chondrocytes in the normal group clustered in the cartilage depression under the microscope. The matrix staining was uniform, while the number of chondrocytes in the case group decreased, and the nucleus was lightly stained or disappeared. Immunohistochemistry assay revealed little or no expression of aminopeptidase N (APN) and leucine aminopeptidase (LAP) in the nucleus pulposus of the normal group, but noticeable APN and LAP expressions in the degenerative intervertebral disc nucleus. Endothelial cells were stained singly or in clusters by CD31-labeled microvascular endothelial growth factor (VEGF). <b>Conclusion:</b> The expression level of various proteolytic enzymes such as aminopeptidase in the intervertebral disc and the linear relationship between microvessel formation and nerve fiber ingrowth in the intervertebral disc are helpful to further explore the molecular level of disc degeneration and pathophysiological mechanisms to aid clinical diagnosis and treatment of such diseases.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"679-684"},"PeriodicalIF":1.9,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2019.1679921","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37469975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginkgolide B improves multiterritory perforator flap survival by inhibiting endoplasmic reticulum stress and oxidative stress.","authors":"Damu Lin,&nbsp;Hongqiang Wu,&nbsp;Zongwei Zhou,&nbsp;Zhenyu Tao,&nbsp;Tanghong Jia,&nbsp;Weiyang Gao","doi":"10.1080/08941939.2019.1676483","DOIUrl":"https://doi.org/10.1080/08941939.2019.1676483","url":null,"abstract":"<p><strong>Background: </strong>The therapeutics used to promote perforator flap survival function induces vascular regeneration and inhibit apoptosis. The present study aimed to explore the potential mechanism of the angiogenesis effects of Ginkgolide B (GB) in perforator flaps. <b>Methods:</b> A total of 72 rats were divided into three groups and treated with saline, GB, or GB + tunicamycin (TM; ER stress activator) for seven consecutive days, respectively. Apoptosis was assayed by determining the Bax/Bcl-2 ratio and caspase-3 level. Endoplasmic reticulum (ER) stress markers (CHOP, GRP78, and caspase-12) were detected by Western blot analysis. Oxidative stress was assessed by measuring the superoxide dismutase activity (SOD) and malondialdehyde (MDA), heme oxygenase-1(HO-1), and nuclear factor erythroid-2-related factor 2 (Nrf2) mRNA levels in the flaps. The percentage flap survival area and blood flow were assessed on postoperative day (POD) 7. Angiogenesis was visualized by hematoxylin and eosin and CD34 staining on POD 7. <b>Results:</b> GB increased the survival of perforator flaps, the flap survival area of GB, GB + TM, and control groups was 90.83 ± 1.93%, 70.93 ± 4.13%, and 62.97 ± 6.50%. GB decreased the Bax/Bcl-2 ratio and caspase-3 level. ER stress-related proteins were downregulated by GB. GB also decreased the MDA level and increased SOD activity, HO-1 and Nrf2 mRNA levels in the flaps. Further, GB induced regeneration of vascular vessels in comparison with saline or GB + TM. <b>Conclusions:</b> GB increased angiogenesis and alleviated oxidative stress by inhibiting ER stress, which increased the survival of perforator flaps. In contrast, GB + TM alleviated angiogenesis and induced oxidative stress by activating ER stress and decreasing the survival of perforator flaps.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"610-616"},"PeriodicalIF":1.9,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2019.1676483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37485266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic Amelioration via Curcumin in Rats following Splenectomy: Lipid Profile, Endothelial and Oxidative Damage.","authors":"Yuksel Altinel,&nbsp;Emin Kose,&nbsp;Aysel Karacaglar,&nbsp;Serdar Demirgan,&nbsp;Volkan Sozer,&nbsp;Guven Ozkaya,&nbsp;Osman Bilgin Gulcicek,&nbsp;Gonul Simsek,&nbsp;Hafize Uzun","doi":"10.1080/08941939.2020.1834651","DOIUrl":"https://doi.org/10.1080/08941939.2020.1834651","url":null,"abstract":"<p><strong>Background: </strong>We investigated the postsurgical effects of splenectomy with additional curcumin therapy, as an antioxidant, anti-inflammatory substance among the lipid profile and histopathological changes.</p><p><strong>Materials and methods: </strong>32 rats were randomly divided into four groups: control group (L): laparotomy, sham group: splenectomy (S), splenectomy group treated with curcumin (SC) and splenectomy group treated with corn oil (SCO) for 28 days. The primary outcomes; total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), oxidized LDL (ox-LDL) very-low-density lipoprotein (VLDL) and lectin-type oxidized LDL receptor 1 (LOX-1), secondary outcomes: nuclear factor kappa B (NF-кB), malondialdehyde (MDA), glutathione peroxidase (GPx), superoxide dismutase (SOD) were measured. Histopathological changes were examined in vascular, intestinal and lung tissues. The analysis was performed by ANOVA.</p><p><strong>Results: </strong>TG, LDL, ox-LDL, and LOX-1 elevated in S group while reduced by curcumin compared with L group (p < 0.05). Serum and tissue levels of NF-кB and MDA were higher in S group and lower in SC group than L group (p < 0.05). Serum and intestinal levels of SOD and GPx increased in L group while reduced by curcumin (p < 0.05). Total histopathological scores of intestinal tissues were higher in S and SCO groups compared to L and SC groups (p < 0.05). No major changes in vascular and lung tissues were observed except the lymphoid follicles which was higher in S and SCO groups compared to L and SC groups (p < 0.05).</p><p><strong>Conclusions: </strong>Curcumin partially improved the lipid profile dysfunction by modulating NF-кB, MDA, SOD, and GPx in splenectomized rats while less likely improving any vascular and alveolar regeneration.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"627-636"},"PeriodicalIF":1.9,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2020.1834651","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38608847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}