Correlation Between Proteolytic Enzymes and Microangiogenesis in Degenerative Intervertebral Disc Nucleus.

Dong Chen, Xin Jiang
{"title":"Correlation Between Proteolytic Enzymes and Microangiogenesis in Degenerative Intervertebral Disc Nucleus.","authors":"Dong Chen,&nbsp;Xin Jiang","doi":"10.1080/08941939.2019.1679921","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation between proteolytic enzymes and microangiogenesis in degenerative intervertebral disc nucleus. <b>Methods:</b> Forty patients with degenerative disc nucleus pulposus who were admitted to our hospital were selected incase group and 20 healthy subjects were selected into the normal group. The specimens from the case group and the control group were collected to observe the degeneration of nucleus pulposus tissues with different degrees of classification, including H&E staining and immunohistochemical staining, and observe cathepsin such as aminopeptidase and vascular endothelial positive cells. The distribution of microvessels was also performed by the Weidner method. <b>Results:</b> After H&E staining, chondrocytes in the normal group clustered in the cartilage depression under the microscope. The matrix staining was uniform, while the number of chondrocytes in the case group decreased, and the nucleus was lightly stained or disappeared. Immunohistochemistry assay revealed little or no expression of aminopeptidase N (APN) and leucine aminopeptidase (LAP) in the nucleus pulposus of the normal group, but noticeable APN and LAP expressions in the degenerative intervertebral disc nucleus. Endothelial cells were stained singly or in clusters by CD31-labeled microvascular endothelial growth factor (VEGF). <b>Conclusion:</b> The expression level of various proteolytic enzymes such as aminopeptidase in the intervertebral disc and the linear relationship between microvessel formation and nerve fiber ingrowth in the intervertebral disc are helpful to further explore the molecular level of disc degeneration and pathophysiological mechanisms to aid clinical diagnosis and treatment of such diseases.</p>","PeriodicalId":284931,"journal":{"name":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","volume":" ","pages":"679-684"},"PeriodicalIF":0.0000,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/08941939.2019.1679921","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of investigative surgery : the official journal of the Academy of Surgical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/08941939.2019.1679921","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2019/12/18 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Objective: To investigate the correlation between proteolytic enzymes and microangiogenesis in degenerative intervertebral disc nucleus. Methods: Forty patients with degenerative disc nucleus pulposus who were admitted to our hospital were selected incase group and 20 healthy subjects were selected into the normal group. The specimens from the case group and the control group were collected to observe the degeneration of nucleus pulposus tissues with different degrees of classification, including H&E staining and immunohistochemical staining, and observe cathepsin such as aminopeptidase and vascular endothelial positive cells. The distribution of microvessels was also performed by the Weidner method. Results: After H&E staining, chondrocytes in the normal group clustered in the cartilage depression under the microscope. The matrix staining was uniform, while the number of chondrocytes in the case group decreased, and the nucleus was lightly stained or disappeared. Immunohistochemistry assay revealed little or no expression of aminopeptidase N (APN) and leucine aminopeptidase (LAP) in the nucleus pulposus of the normal group, but noticeable APN and LAP expressions in the degenerative intervertebral disc nucleus. Endothelial cells were stained singly or in clusters by CD31-labeled microvascular endothelial growth factor (VEGF). Conclusion: The expression level of various proteolytic enzymes such as aminopeptidase in the intervertebral disc and the linear relationship between microvessel formation and nerve fiber ingrowth in the intervertebral disc are helpful to further explore the molecular level of disc degeneration and pathophysiological mechanisms to aid clinical diagnosis and treatment of such diseases.

退行性椎间盘核中蛋白水解酶与微血管生成的关系。
目的:探讨退行性椎间盘核中蛋白水解酶与微血管生成的关系。方法:选取我院收治的退变性椎间盘髓核患者40例为病例组,健康者20例为正常组。取病例组和对照组标本,观察不同分级髓核组织的退变情况,包括H&E染色和免疫组化染色,观察组织蛋白酶如氨基肽酶和血管内皮阳性细胞。微血管的分布也采用Weidner法。结果:H&E染色后,显微镜下正常组软骨细胞聚集在软骨凹陷处。基质染色均匀,病例组软骨细胞数量减少,细胞核轻度染色或消失。免疫组化检测显示,正常组髓核中氨肽酶N (APN)和亮氨酸氨基肽酶(LAP)表达很少或不表达,但在退行性椎间盘核中有明显的APN和LAP表达。用cd31标记的微血管内皮生长因子(VEGF)对内皮细胞进行单个或成团染色。结论:椎间盘内各种蛋白水解酶如氨基肽酶的表达水平以及椎间盘微血管形成与神经纤维长入的线性关系,有助于进一步探讨椎间盘退变的分子水平和病理生理机制,帮助临床诊断和治疗此类疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信