Menoufia Journal of Agricultural Biotechnology最新文献

{"title":"BIOCHEMICAL STUDIES ON THE EFFECT OF ONION ON SOME BLOOD PARAMETERS OF RATS","authors":"M. A. Habib, K. M. Taha, A. Sheta","doi":"10.21608/mjab.2021.207625","DOIUrl":"https://doi.org/10.21608/mjab.2021.207625","url":null,"abstract":"The objective of the present research was to study chemical composition, phenolic compounds and flavonoids of onion. Onion contained total carbohydrates 70.7%, crude protein 7.35 %, total lipids 1.43%, total ash 2.91% fibers 7.91 % and moisture 9.7% based the dry sample. Total phenols in onion bulbs was 35.23 mg /100g., while the total flavonoids was 23.18 mg/100g. HPLC results showed that onion contained 17 phenolic compounds. There were a high percentage of (benzoic acid 905 mg/kg), caticol (649 mg/kg), risvertol (425 mg/kg), perogalol (157 mg/kg) and naringinin (96 mg/kg). that help to improve some blood parameters such as liver function enzymes, kidneys function, lipids and glucose levels. Onion powder as well as ethanolic and water extracts of onion bulbs were prepared which used in the present treatments. Blood parameters of rats were tested using the methods of A.O.A.C.; (2005). Onion powder used as a treatment with concentration of 2 g% and 4 g% of diet. The ethanolic extract of onion used as a treatment with concentration of 1 g/L and 2 g/L. While water extract used as a treatment with concentration of 1 g/L and 2 g/L for hyperlipidemic rats. Blood parameters of rats were tested after the treatments. Data were subjected to statistical analysis using the SPSS (Software version no. 20). Differences between extracts were tested. The best results were obtained by comparisons of the results shown. Treatments with onion powder and its extracts were beneficial to control the hyperlipidemia, and improved the levels of some blood parameters of rats such as, total cholesterol, HDL-c, LDL-c, TG, ALT, AST, creatinine, urea, uric acid, blood glucose.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"168 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132822133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF SPRAYING BLUE GREEN ALGAE (CYANOBACTERIA) EXTRACTS ON HYBRID RICE SEED PRODUCTION","authors":"H. El-zawawy, E. A. Abd El-aziz, H. Hamad, E. Arafat","doi":"10.21608/mjab.2021.207623","DOIUrl":"https://doi.org/10.21608/mjab.2021.207623","url":null,"abstract":"Two isolates of cyanobacteria (Anabaena oryzae, Nosto centophytum) were isolated from agricultural wastewater from the experimental farm of Sakha Research Station, Sakha, Kafr EL Sheikh, Egypt for testing their ability to fix atmospheric nitrogen and produce hormones and its effect on hybrid rice seed production by spraying the isolates extract and their mixture on the rice plants. Isolates were varied in their capacity in the extracellular-nitrogen, Intracellular secreted, produce hormones and dry weight of the cyanobacteria isolates (mg/100 ml-culture) where the lowest content was for isolate Anabaena oryzae and the highest was for Nostoc centophytum and mixture. The aim of the present investigation is to identify the effect of cyanobacteria extract isolated from Egyptian waste water on hybrid rice seed production. The used cytoplasmic male sterile lines were IR69625A and G46A and the restorer line was G.178. Application of mixture between Anabaena oryzae and Nostoc centophytum produced the highest values in most studied characters such as seed yield in both seasons.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122473487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HEPATOPROTECTIVE EFFECTS OF TURMERIC AND MILK THISTLE SEED FLOUR AGAINST ETHANOL LIVER DAMAGE IN WISTER RATS","authors":"F. El-Shouny, S. K. El-Kadousy, S. M. El-Gawad, Kh. A. El-Khalwy","doi":"10.21608/mjab.2021.207624","DOIUrl":"https://doi.org/10.21608/mjab.2021.207624","url":null,"abstract":"Chemical composition of milk thistle seed flour )MTSF) and turmeric (T) were investigated, for milk thistle seed flour, moisture (8.0%), protein (13.0 %), carbohydrates (27.55%), crude lipid (15.0%), ash (10.45%), and crude fiber (26.0%), while turmeric has moisture (8.1%) , protein (9.3 %), carbohydrates (57%), crude lipid (17.0%), ash (6.0%) and crude fiber (2.6%). For total phenol, the highest value in turmeric (11.57mg GAE/g) while milk thistle seed flour (3.39 mg GAE/g)) also total flavonoid for turmeric (4.9mg) while and in milk thistle seed flour (2.34mg). DPPH for milk thistle recorded the highest percent of (FRSA) 98.5 % and turmeric recorded lowest present 82.2% in concentration 100 μg/ml. Reducing power for milk thistle recorded 0.651 but turmeric (T) recorded 0.502 in concentration 100 μg/ml . The identification of phenolic compounds for plant extracts were investigated by HPLC ,the highest content in milk thistle seed flour (MTSF) was Benzoic acid (103.33 ppm) while the lowest content was Caffeine(1.07 ppm) but in turmeric(T) the highest content was Benzoic acid (208.41 ppm), while the lowest content was quercitin (3.08 ppm).The level of plasma aspartate aminotransferase (AST) , alanine aminotransferase (ALT) , alkaline phosphatase (ALP) , total bilirubin , total protein and albumin and plasma antioxidant state SOD , CAT, GPx and MDA were determined to assay hepatotoxicity.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126822732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE STUDY BETWEEN GINGER, CINNAMON AND CHAMOMILE ESSENTIAL OILS (PHYSICAL PROPERTIES, CHEMICAL PROPERTIES AND CHEMICAL COMPOSITION)","authors":"H. Mohamed, Y. Ashoush, K. M. Taha, Medhat Mostafa Abozid","doi":"10.21608/mjab.2021.193160","DOIUrl":"https://doi.org/10.21608/mjab.2021.193160","url":null,"abstract":"Current study designed to compare three important essential oils (Ginger, cinnamon and chamomile) commonly used in Egypt. For this purpose, the essential oils were extracted from ginger rhizomes, cinnamon sticks and chamomile flowers by hydrodistillation; then the physical and chemical properties of these oils were estimated, as well as the identification of the active compounds by using GC-MS. Cinnamon oil recorded the highest values in both specific gravity and refractive index, while ginger oil was the highest in solubility in 80% ethanol. Chamomile oil recorded the highest value in acid number, while the ginger oil was the highest in both saponification number and ester number. GC-MS results identify 12 compounds in ginger oil, 6 compounds in cinnamon oil and 10 compounds in chamomile oil. On the other hand, zingiberene (33.2%) cinnamaldehyde (36.1%) and bisabolol oxide (46.71%) were the main major compounds in ginger oil, cinnamon oil and chamomile oil, respectively. In conclusion the obtained results found that, there are a big difference in physical and chemical properties between these essential oils, they also showed a very different chemical composition which leads to an expected difference in their biological effects.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131432414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A COMPARATIVE STUDY BETWEEN GREEN TEA (CAMELLIA SINENSIS) AND COMMON SAGE (SALVIA OFFICINALIS) (CHEMICAL COMPOSITION AND ACTIVE COMPOUNDS)","authors":"S. Draz, A. Ali, K. Hussein, A. Afifi","doi":"10.21608/MJAB.2021.169121","DOIUrl":"https://doi.org/10.21608/MJAB.2021.169121","url":null,"abstract":"The purpose of this research is to study the chemical composition and effective compounds of leaves of green tea and sage as one of the traditional medicinal plants. It was found that green tea leaves collected from the local market contain 5.68 % moisture, 2.35% ash, 14.57 % protein, 8.55 % lipids, and 2.3% crude fiber. While sage leaves contained 8.19, 2.17, 12.21, 3.33, and 2.6 % moisture, ash, protein, lipids, and crude fiber, respectively. It was also found that green tea leaves contain 0.376 % Polyphenols and 0.188 % Flavonoids, while sage leaves contain 0.262 and 0.132 % Polyphenols and Flavonoids, respectively. HPLC showed that the aqueous extract of green tea leaves contains eighteen compounds of Polyphenols, fifteen compounds of them were identified. While the aqueous extract of sage leaves contains twenty-one compounds, nineteen of them were identified.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126187127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENETIC ASSESSMENT OF LEAF RUST RESISTANCE IN SOME WHEAT VARIETIES","authors":"R. A. Eissa, A. Fahmi, A. M. El-Zanaty, W. M. E. Orabey","doi":"10.21608/MJAB.2020.172399","DOIUrl":"https://doi.org/10.21608/MJAB.2020.172399","url":null,"abstract":"The experiments of this study were carried out through four growing seasons i.e., 2015/16 to 2018/19 at the farm of the faculty of Agriculture, Shibin El-Kom, Menoufia University.Using10 Egyptian wheat varieties,16 wheat promising lines from (CIMMYT) and 20 adult plant leaf rust resistance genes (Lr). lines Lr were used as (male) for crosses with the wheat cultivars (female) to obtain the F1 grains to produce F2 grains and perform genetic analysis. Recording final rust severity (FRS), rate of disease increase (r-value) and the area under disease progress curve (AUDPC) from varieties and lines to responses leaf rust resistance. While STS marker specific for Lr19 and Lr28 genes were performed on the ten wheat varieties and the sixteen promising lines. The responses of rust severity and infection type of the 20 Lr strains were divided into three groups; I resistant genes i.e., Lr17, Lr18, Lr28, Lr 38 and Lr42, II susceptible genes i.e., Lr2c, Lr9, Lr14b, Lr24, Lr34, Lr35, Lr36, Lr37, Lr44 and Lr45, and III genes with variable reactions i.e. Lr2a, Lr19, Lr29, Lr30 and Lr39. Results suggested that genes of group I are important for breeding for leaf rust resistance under Egyptian field conditions. The ten wheat cultivars exhibited low values of FRS and AUDPC except Sids1, and no variety has high rate of (r-value). These cultivars except Sids1 have a good level of adult plant resistance to leaf rust and can be used as resistance sources, 16 wheat promising lines gave lowest values of (FRS), (r-value) and (AUDPC), and consequently they can be used in breeding programs. Genetic analysis showed that Sids1 did not have any of genes under study. However, Gemmeiza 7, Gemmeiza 9, Gemmeiza 10, Gemmeiza 11, Gemmeiza 12, Misr 1, Misr 2, Giza168 and Giza 171 have several Lr genes. Using STS marker specific for Lr19 and Lr28 genes, Lr19 was identified in Gemmeiza 7 and Gemmeiza 11 and Lr28 detected in Gemmeiza 9 and Gemmeiza 10, while Lr19 detected in 13 promising lines, Lr28 found only in five of them. These results showed that molecular markers for Lr genes might be a useful tool in marker-assisted breeding (MAS) for developing Egyptian wheat cultivars.","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126840118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOCHEMICAL STUDIES ON MAHOGANY SEEDS","authors":"M. Hammam, G. Khalil, S. S. A. ElGawad","doi":"10.21608/mjab.2020.127526","DOIUrl":"https://doi.org/10.21608/mjab.2020.127526","url":null,"abstract":"The present study was designed to investigate the chemical composition of Swietenia mahagoni seeds , identification of phenolic compounds in acetone extract in  mahagoni seeds, studying the fatty acids composition, and evaluation of mahagoni extracts and oil  on the glucose level of diabetes  rats. The chemical composition of seeds were moisture (3.6 %), crude fiber (14 %), ash (3%), crude protein (13 %), crude fat (7.4 %) and total carbohydrate (62.6 %). The fatty acids composition were lignoceric acid (C24) 38.23% followed by stearic acid (C18) 35.69%, meanwhile the unsaturated fatty acid was linoleic acid (C18:2), which accounted for ( 26.08 %). The phenolic compound in acetone extract showed that menthol and nerolidol are the major phenolic ( 12.32 and 10.06 % respectively ). Ttreatment with Swietenia mahagoni seed extracts and oil decreased significantly glucose level , also decreased significantly GOT , GPT , ALP , urea , creatinine and malondialdehyde level   as compared with  hyperglycemic group .","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"422 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122464533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SWIETENIA MAHAGONI SEEDS ATTENUATE HYPERGLYCEMIA AND PROTECT LIVER IN ALLOXAN-INDUCED DIABETIC RATS","authors":"M. Hammam, G. Kalil, S. Sayed, I. Mohamed","doi":"10.21608/mjab.2020.112874","DOIUrl":"https://doi.org/10.21608/mjab.2020.112874","url":null,"abstract":"The present study was designed to investigate the chemical composition of Swietenia mahagoni seeds , identification of phenolic compounds in acetone extract in  mahagoni seeds, studying the fatty acids composition, and evaluation of mahagoni extracts and oil  on the glucose level of diabetes  rats. The chemical composition of seeds were moisture (3.6 %), crude fiber (14 %), ash (3%), crude protein (13 %), crude fat (7.4 %) and total carbohydrate (62.6 %). The fatty acids composition were lignoceric acid (C24) 38.23% followed by stearic acid (C18) 35.69%, meanwhile the unsaturated fatty acid was linoleic acid (C18:2), which accounted for ( 26.08 %). The phenolic compound in acetone extract showed that menthol and nerolidol are the major phenolic ( 12.32 and 10.06 % respectively ). Ttreatment with Swietenia mahagoni seed extracts and oil decreased significantly glucose level , also decreased significantly GOT , GPT , ALP , urea , creatinine and malondialdehyde level   as compared with  hyperglycemic group .","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131921578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDIES ON PROMOTER EXPRESSION OF TOXINS GENES IN SOME FOOD POISONING BACTERIA.","authors":"W. Mahmoud, A. El-beltagy, S. F. A. Allah","doi":"10.21608/mjab.2020.110247","DOIUrl":"https://doi.org/10.21608/mjab.2020.110247","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131358766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"USE OF MICROBIAL LIPOPEPTIDES AGAINST SOME PLANT PATHOGENIC MICROORGANISMS","authors":"W. Mahmoud, A. El-beltagy, S. F. A. Allah","doi":"10.21608/mjab.2020.110244","DOIUrl":"https://doi.org/10.21608/mjab.2020.110244","url":null,"abstract":"","PeriodicalId":280782,"journal":{"name":"Menoufia Journal of Agricultural Biotechnology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114333079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}