Chemosphere最新文献

{"title":"Importance of the envelope in Escherichia coli resistance to lithium","authors":"Nicolas Fierling ,&nbsp;Patrick Billard ,&nbsp;Antoine Dluzniewski ,&nbsp;Bénédicte Sohm ,&nbsp;Pascale Bauda ,&nbsp;Damien Blaudez","doi":"10.1016/j.chemosphere.2025.144234","DOIUrl":"10.1016/j.chemosphere.2025.144234","url":null,"abstract":"<div><div>The increasing use of lithium (Li) in emerging technologies has prompted concerns about its effects on living microorganisms. To enhance our understanding of the bacterial cytotoxicity of Li, we conducted a deletomic analysis using the bacterial model <em>Escherichia coli</em>. A screen of 3,985 knockout mutants under Li stress highlighted 27 Li-sensitive and 15 Li-resistant mutants. The synthesis of peptidoglycan and the capsule, along with the secretion of colanic acid, contributed to resistance to Li. Ribosomes and the stringent response also seem to play a role in mitigating Li cytotoxicity. A cross-metal comparison revealed that the Li-sensitive phenotype of the mutants was shared with Ca, whereas the resistant phenotype was shared with Mg, Na and K. Moreover, this allowed the identification of Δ<em>acrA</em> as a Li sensitivity-specific mutant. AcrA is a subunit of the AcrAB–TolC efflux pump, which is responsible for the efflux of various xenobiotics. We demonstrate that Δ<em>acrB-</em>Δ<em>tolC</em> accumulates approximately 1.5 times more Li than the WT, indicating that this pump could also facilitate the efflux of Li. This study offers a more comprehensive insight into the mechanisms involved in the Li response in <em>E. coli</em>.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144234"},"PeriodicalIF":8.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological and genotoxicological assessment of water extracts of sewage sludge and other biogenic wastes: A piece of the SLURP jigsaw puzzle","authors":"Carlotta Alias ,&nbsp;Donatella Feretti ,&nbsp;Ilaria Zerbini ,&nbsp;Roberta Pedrazzani ,&nbsp;Marta Domini ,&nbsp;Giorgio Bertanza","doi":"10.1016/j.chemosphere.2025.144175","DOIUrl":"10.1016/j.chemosphere.2025.144175","url":null,"abstract":"<div><div>Given the considerable quantities of biogenic matrices employed in agricultural applications, there is growing concern about the potential negative effects resulting from the presence of harmful contaminants. The project “SLURP - SLUdge Recovery in Agriculture: Environment and Health Protection” planned a multi-stage approach in which the application of a wide battery of bioassays was proposed as an effective tool to measure the direct interaction of matrices with the different components of the ecosystem, from the molecular to the whole organism level. The aim of the present study, which is a part of the “SLURP” project, was to characterise the toxicological and genotoxicological properties of water extracts from biogenic wastes using several assays based on plant, bacterial and human cells. The aqueous extracts of four sewage sludges, a liming material, two manure slurries of swine and bovine origin, a digestate from bovine manure and a compost were chemically characterised for inorganic ions and heavy metals. Then the extracts were analysed using tests on <em>A.cepa, C.sativus</em>, <em>L.sativum</em>, <em>S.typhimurium</em> and human hepatoma cell line (HepG2) to assess toxicity (seed germination, root elongation, proliferation), mutagenicity and genotoxicity (primary DNA damage, chromosomal aberrations). The extracts exhibited chemical heterogeneity. Ammonia nitrogen, Ca²⁺, Fe and Zn were the most abundant elements. Toxic effects were caused on <em>A.cepa</em> and <em>L.sativum</em> by all extracts, while there were non-toxic effects on human cells. Genotoxic effects on <em>A.cepa</em> and <em>L.sativum</em> were instead caused by almost all the extracts, at least at the highest dose tested, while only four samples from one sewage sludge, liming material, digestate, and compost, caused DNA damage on human cells. None of the extracts induced mutagenic effects in <em>S.typhimurium</em>. A comprehensive interpretation of these results can only be achieved through the integrated evaluation of all eco-toxicological and chemical data obtained throughout the entire project.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144175"},"PeriodicalIF":8.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physics-informed neural networks for enhanced reference evapotranspiration estimation in Morocco: Balancing semi-physical models and deep learning","authors":"Chouaib El Hachimi ,&nbsp;Salwa Belaqziz ,&nbsp;Saïd Khabba ,&nbsp;Andre Daccache ,&nbsp;Bouchra Ait Hssaine ,&nbsp;Hasan Karjoun ,&nbsp;Youness Ouassanouan ,&nbsp;Badreddine Sebbar ,&nbsp;Mohamed Hakim Kharrou ,&nbsp;Salah Er-Raki ,&nbsp;Abdelghani Chehbouni","doi":"10.1016/j.chemosphere.2025.144238","DOIUrl":"10.1016/j.chemosphere.2025.144238","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Reference evapotranspiration (&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;E&lt;/mi&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;) is essential for agricultural water management, crop productivity, and irrigation systems. The Penman-Monteith (PM) equation is the standard method for estimating &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;E&lt;/mi&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, but its data-intensive nature makes it impractical, especially in situations where the cost of full standardized weather station is prohibitive, maintenance is inadequate, or data quality and continuity are compromised. To overcome those limitations, various semi-physical (SP) and empirical models with limited weather parameters were developed. In this context, artificial intelligence methods for &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;E&lt;/mi&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; estimation are gaining more attention, balancing simplicity, minimal data requirements, and high accuracy. However, their data-driven nature raises concerns regarding explainability, trustworthiness, adherence to bio-physical laws, and reliability in operational settings. To address this issue, this paper, inspired by the emerging field of Physics-Informed Neural Networks (PINNs), evaluates the integration of SP models into the loss function during the learning process. The new residual loss combines two losses –the data-driven loss and the loss from SP– through a &lt;em&gt;θ&lt;/em&gt; parameter, allowing for a convex combination. In-situ agrometeorological data were collected at four automatic weather stations in Tensift Watershed in Morocco, including air temperature (&lt;em&gt;Ta&lt;/em&gt;), solar radiation (&lt;em&gt;Rs&lt;/em&gt;), relative humidity (&lt;em&gt;RH&lt;/em&gt;), and wind speed (&lt;em&gt;Ws&lt;/em&gt;). The study integrates Priestley-Taylor (PT), Makkink (MK), Hargreaves-Samani (HS), and Abtew (AB), under four scenarios of data availability levels: (1) &lt;em&gt;Ta&lt;/em&gt;, &lt;em&gt;Rs&lt;/em&gt; and &lt;em&gt;RH&lt;/em&gt;; (2) &lt;em&gt;Ta&lt;/em&gt; and &lt;em&gt;Rs&lt;/em&gt;; (3) only &lt;em&gt;Ta&lt;/em&gt;; and (4) only &lt;em&gt;Rs&lt;/em&gt;. The investigation begins with quality-controlling the data and studying the driving factors of &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;E&lt;/mi&gt;&lt;mi&gt;T&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;. Next, the SP models were calibrated using the CMA-ES optimization algorithm. The proposed PINN was trained and evaluated, first, for the equal contribution scenario (&lt;em&gt;θ&lt;/em&gt; = 0.5) and then for &lt;em&gt;θ&lt;/em&gt; in the interval [0, 1] with a step of 0.2, thus analyzing the impact of &lt;em&gt;θ&lt;/em&gt; on the PINN performance. For the equal contribution, the results showed that the integration had improved the PINN performance in all scenarios in terms of the &lt;em&gt;RMSE&lt;/em&gt; and &lt;em&gt;R&lt;/em&gt;&lt;sup&gt;&lt;em&gt;2&lt;/em&gt;&lt;/sup&gt;, surpassing the fully data-driven model (&lt;em&gt;θ&lt;/em&gt; = 0) and the baseline model (&lt;em&gt;θ&lt;/em&gt; = 1). Additionally, for all &lt;em&gt;θ&lt;/em&gt; within the interval [0.2, 0.8], the PINN required less training to reach optimal values. Finally, the optimal &lt;em&gt;θ&lt;/em&gt; values were determined for each scenario using CMA-ES and were 0.258, 0.771, 0.7226 and 0.169 for PT, MK, HS and ","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144238"},"PeriodicalIF":8.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring the stability of aerobic granular sludge under increasing fractions of slowly biodegradable substrate using quantitative image analysis","authors":"Sérgio A. Silva ,&nbsp;M. Salomé Duarte ,&nbsp;António L. Amaral ,&nbsp;Eugénio C. Ferreira ,&nbsp;M. Madalena Alves ,&nbsp;Daniela P. Mesquita","doi":"10.1016/j.chemosphere.2025.144233","DOIUrl":"10.1016/j.chemosphere.2025.144233","url":null,"abstract":"<div><div>This work investigates the effects of increasing fraction of slowly biodegradable chemical oxygen demand (sbCOD) on the morphology, stability, and performance of aerobic granular sludge (AGS) used for wastewater treatment. A sequencing batch reactor (SBR) was supplied with synthetic wastewater containing acetate as readily biodegradable COD (rbCOD) and increasing concentrations of oleate as slowly biodegradable carbon source. The sbCOD fraction was gradually increased, reaching up to 50% of the total influent biodegradable COD (bCOD). Quantitative image analysis (QIA) revealed a significant shift in granule morphology and size distribution due to increasing sbCOD fractions. Larger granules (Deq &gt;1.0 mm) become predominant due to the washout of smaller granules (Deq &lt;1.0 mm), which evidenced deterioration in several structural parameters. In contrary, larger granules maintained stable compactness, robustness, and extent. These morphological and size distribution changes were concomitant with variations in reactor performance: total inorganic nitrogen (TIN) removal efficiency improved up to 94%, due to enhanced denitrification capacity, supported by the predominance of larger granules and increase in granules size at higher sbCOD fractions. In contrast, P-PO₄^3- removal efficiency declined, associated with the leakage of rbCOD to the aerobic phase, filamentous growth, and deteriorated sludge settling properties. These findings highlight the complex interactions between oleate characteristics, AGS morphology, and reactor performance, emphasizing the need for optimized strategies to mitigate process instability in AGS systems treating lipid-rich wastewater, ensuring sustainable and efficient wastewater treatment in real-world applications.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144233"},"PeriodicalIF":8.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Source identification and quantification of real-world PAH contributions from traffic-related exhaust and non-exhaust emission sources using the EFECT method","authors":"Duran Karakaş ,&nbsp;Ercan Berberler ,&nbsp;Tuğçe Demir ,&nbsp;Melike B. Bayramoğlu Karşı ,&nbsp;Serpil Yenisoy","doi":"10.1016/j.chemosphere.2025.144237","DOIUrl":"10.1016/j.chemosphere.2025.144237","url":null,"abstract":"<div><div>Proper identification of sources and their contribution to atmospheric gaseous and particle-bound PAHs from traffic-related sources are vital for controlling and reducing emissions in the urban atmosphere to improve environmental health. For this purpose, we used the EFECT method to quantify the PAH contributions from the pre-defined vehicular sources, namely, road dust resuspension, exhaust (tailpipe), and non-exhaust sources in a rural highway tunnel. We successfully quantified the relative source contributions of particle-bound and total PAHs (particulate plus gaseous PAHs) to the atmospheric concentrations. Study results showed that exhaust emissions were observed to be the most important emission source for both particle-bound and total PAHs. This study showed that exhaust emission sources contribute 92.4% of IcdP, 90.3% of Phe, 89.9% of AN, 89.1% of BkF, 88.2% of Np, 87.1% of Ane, 86.3% of DahA, 85.7% of BghiP, and 82.8% of Flr to atmospheric concentrations in the tunnel environment. Road dust resuspension contributed mainly to TSP (total suspended particulate matter) concentrations of BaA (29.1%), Py (28.9%), BbF (21.0%), and BaP (21.0%).</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144237"},"PeriodicalIF":8.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of perfluoroalkyl substance (PFAS) on vitamin D biomarkers in a highly exposed population of the Veneto Region in Italy","authors":"Andrea Di Nisio ,&nbsp;Luca De Toni ,&nbsp;Cristina Canova ,&nbsp;Mirko Berti ,&nbsp;Achille Di Falco ,&nbsp;Rinaldo Zolin ,&nbsp;Anna Maria Bettega ,&nbsp;Iva Sabovic ,&nbsp;Alberto Ferlin ,&nbsp;Carlo Foresta","doi":"10.1016/j.chemosphere.2025.144230","DOIUrl":"10.1016/j.chemosphere.2025.144230","url":null,"abstract":"<div><div>Perfluoroalkyl substances (PFASs) raise concerns about their environmental accumulation. Experimental data have suggested that PFASs interfere with bone metabolism from the early stages of life. However, mechanisms underlying this association are unclear. The aim of this study was to evaluate the possible association between environmental exposure to PFAS and vitamin D (VitD), serum calcium and parathyroid hormone (PTH) levels in subjects residing in high-exposure area of the Veneto Region of Italy. In this cross-sectional observational study, 1174 subjects who previously adhered to the 2016–2018 Regional Surveillance Plan for plasma levels of PFASs were recalled in 2023 and evaluated for demographic, anthropometrics and blood analyses. Data on nutritional habits and VitD supplementation were obtained by a dedicated questionnaire. Serum concentrations of PFASs, calcium, 25-hydroxy-vitamin D (25OH-VitD) and PTH were determined from blood sampling. Perfluorooctanoic acid (PFOA), perfluorooctanesulfonate (PFOS) and perfluorohexanesulfonic acid (PFHxS) were the only three PFASs, of 12, quantifiable in at least 90% of the samples and considered for further analyses. Generalized additive models, using linear regression and smoothing thin plate splines, detected a positive association between serum calcium and all considered PFAS (PFOA: β = 0.03; CI 95% 0.01–0.06; PFOS: β = 0.06; CI 95% 0.02–0.09, PFHxS: β = 0.04; CI 95% 0.01–0.06). Estimated degrees of freedom (EDF) analysis showed the approximately linear association between serum calcium with PFOA (EDF = 1.89) and PFHxS (EDF = 1.21), but not for PFOS (EDF = 3.69). Differently, PFAS levels showed no association with either 25-hydroxy-vitamin D or PTH, except for ln-transformed 25OH-D and PFOS (β = 0.04; CI 95% 0.00–0.08). Stratified analyses confirmed the positive association between all considered PFAS and calcium in subjects not taking a VitD supplementation. Results show that high exposure levels to PFAS may interfere with calcium metabolism, independently of lifestyle and dietary factors. Further elucidation on the mechanisms underlying calcium homeostasis disruption, including multiple binding-equilibrium with serum albumin, remains to be addressed.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144230"},"PeriodicalIF":8.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143436500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro- and nano-plastics pollution in the marine environment: Progresses, drawbacks and future guidelines","authors":"Sesan Abiodun Aransiola ,&nbsp;Munachimso Odenakachi Victor-Ekwebelem ,&nbsp;Bryan Xavier Daza ,&nbsp;Peter Olusakin Oladoye ,&nbsp;Yakubu Adekunle Alli ,&nbsp;Abayomi Bamisaye ,&nbsp;Adejoke Blessing Aransiola ,&nbsp;Samuel Oluwadadepo Oni ,&nbsp;Naga Raju Maddela","doi":"10.1016/j.chemosphere.2025.144211","DOIUrl":"10.1016/j.chemosphere.2025.144211","url":null,"abstract":"<div><div>Marine pollution by micro/nanoplastics (M/NPs) has emerged as a critical global issue, with widespread ecological and economic consequences. Numerous studies have investigated M/NPs pollution in marine environments, but there remains a need to assess progress, identify challenges, and propose future strategies. This review provides updated insights into marine M/NPs, including their sources, detection methods, global data from diverse marine ecosystems, and the challenges in mitigating pollution. The review reveals that the ocean harbors approximately 5.25 trillion plastic debris pieces, with a total of 50–75 trillion plastic and microplastic particles, with deep-sea regions containing up to 4 billion plastic microfibers per square kilometer. Human activities, including industrial practices and aquaculture, are major contributors to M/NPs pollution, which threatens 17% of marine species and incurs an economic loss of 6–9 billion USD. M/NPs are found across various marine habitats, including shorelines, sea floors, water columns, biota, and floating debris. Analyzing nanoplastics is particularly challenging due to their heterogeneous aggregation with other contaminants and their much lower concentrations than natural particles. Key drawbacks in addressing M/NPs pollution include inadequate funding, insufficient regulations, and a lack of policy frameworks on the prevalence, distribution, and sources of M/NPs. There is an increasing focus on utilizing innovative technologies such as artificial intelligence (AI) to monitor, assess risks, and predict the spread of M/NPs. Therefore, urgent global cooperation, involving all stakeholders and the general public, is essential. Additionally, integrating scientific and engineering methods, along with AI technologies, is crucial for monitoring and controlling M/NPs pollution and developing sustainable solutions.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144211"},"PeriodicalIF":8.1,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143446022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicological efficiency of Cr(VI) removal treatment with reductive biogenic iron-based material determined by amphibian larval bioassays","authors":"Gabriela S. Paronetto,&nbsp;Gabriela Svartz,&nbsp;Melisa S. Olivelli,&nbsp;Jose L. Marco-Brown,&nbsp;Roberto Candal","doi":"10.1016/j.chemosphere.2025.144215","DOIUrl":"10.1016/j.chemosphere.2025.144215","url":null,"abstract":"<div><div>Due to the toxic effects of chromium, its high environmental persistence, and potential for bioaccumulation, it is imperative to develop sustainable strategies for managing its contamination. In the present study, the synthesis time of supported biogenic iron-based microparticles (BioFe-MMT) and their performance in the Cr(VI) removal process were analyzed. Its lethal and sublethal ecotoxicological effects were subsequently evaluated through standardized bioassays using amphibian <em>Rhinella arenarum</em> larvae exposed to BioFe-MMT and Cr(VI) before and after the removal treatment. Results demonstrated that BioFe-MMT biosynthesized for 3 weeks had smaller particle size and better Cr(VI) and total Cr removal efficiency (&gt;99% and &gt;91%, respectively) compared to longer synthesis times. Ecotoxicological bioassays showed that Cr(VI) caused a significant increment of lethal toxicity to larvae with 50% Lethal Concentration (LC 50) at 96 and 504 h of 25.1 and 0.04 mg L⁻¹, respectively. Several sublethal effects were observed such as reduced body size, wavy and underdeveloped tail, and behavioral disorders. This toxicity could be explained by the Bioconcentration Factor (BCF) at 96 h, which was 12.26 L kg⁻¹. On the other hand, BioFe-MMT and the supernatant obtained after the treatment had negligible toxic effects. Larvae exposed to the sludge obtained after Cr(VI) removal treatment (Cr-BioFe-MMT) presented significant mortality from 216 h, which was explained by Cr(VI) desorption from the Cr-BioFe-MMT sludge. These results highlight the BioFe-MMT potential use in Cr(VI) removal due to its efficiency and the reduced toxicity of the treated solution.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144215"},"PeriodicalIF":8.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"M8OI toxicity is associated with an inhibition of ubiquinone reduction by complex I in the mitochondrial electron transport chain","authors":"Tarek M. Abdelghany ,&nbsp;Jessica Bosak ,&nbsp;Alistair C. Leitch ,&nbsp;Alex Charlton ,&nbsp;Lanyu Fan ,&nbsp;Fahad A. Aljehani ,&nbsp;Omar H. Alkhathami ,&nbsp;Shireen A. Hedya ,&nbsp;Satomi Miwa ,&nbsp;Agnieszka K. Bronowska ,&nbsp;Judy Hirst ,&nbsp;Matthew C. Wright","doi":"10.1016/j.chemosphere.2025.144213","DOIUrl":"10.1016/j.chemosphere.2025.144213","url":null,"abstract":"<div><div>Methylimidazolium ionic liquids (MILs) are solvents used in an increasing variety of industrial applications. Recent studies identified the 8C MIL (M8OI) contaminating the environment, detected exposure in humans and proposed M8OI to be a potential trigger for the autoimmune liver disease primary biliary cholangitis (PBC). To gain a better understanding of any PBC trigger mechanism(s), the interaction of M8OI with mitochondria has been examined. M8OI inhibited oxygen consumption in intact cells and induced cell death (IC_50%–10 μM). Results from permeabilized cells indicated M8OI inhibits the mitochondrial electron transport chain at complex I, not complexes II, III or IV. Accordingly, succinate supported mitochondrial oxygen consumption and reduced cell death in the presence of M8OI. M8OI inhibited NADH oxidation by both mitochondrial membranes and purified complex I with IC_50% values of 470 μM and 340 μM respectively. Based on direct determinations of M8OI in non-mitochondrial and mitochondrial compartments, toxic M8OI concentrations were estimated to result in mitochondrial concentrations commensurate with complex I inhibition. Mitochondrial accumulation followed by complex I inhibition is therefore a possible molecular initiating event for M8OI-dependent cell death. NADH oxidation by purified complex I in combination with a flavin-site electron acceptor was not inhibited by M8OI, indicating no interaction of M8OI at the NADH-binding active site. Modelling supported M8OI binding to the ubiquinone-binding site. By inhibiting turnover, M8OI also gave rise to increases in complex-I-linked reactive oxygen species. However, inhibitors of oxidative stress did not affect M8OI-mediated cell death. The metabolic consequences of M8OI-mediated complex I inhibition, not increased reactive oxygen species production, are therefore the likely cause of apoptotic cell death. Understanding the effects on complex I and the pathways activated and leading to cell death may be informative regarding mitochondrial stress, cell death and diseases such as PBC.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144213"},"PeriodicalIF":8.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomonitoring toxic and essential element accumulation in trout in the as pontes mine pit lake (NW Spain)","authors":"Marta López-Alonso ,&nbsp;Marta Miranda ,&nbsp;Inmaculada Orjales ,&nbsp;Víctor Pereira ,&nbsp;Belén Larrán ,&nbsp;Ruth Rodríguez-Bermúdez ,&nbsp;Cathaysa Veiras ,&nbsp;Álvaro Robles ,&nbsp;David Bravo ,&nbsp;Ricardo Juncosa ,&nbsp;Carlos Herrero-Latorre","doi":"10.1016/j.chemosphere.2025.144212","DOIUrl":"10.1016/j.chemosphere.2025.144212","url":null,"abstract":"<div><div>This study investigated the accumulation of toxic and essential elements in trout from a pit lake situated in the surroundings of the abandoned As Pontes de García Rodríguez lignite mine (NW Spain). The element concentrations were compared with those measured in fish from upstream of the River Eume and from a local fish farm. Liver and muscle samples from fish captured in the lake (n = 16), river (n = 14) and fish farm (n = 10) captured in March–April 2022 were acid digested and analyzed by ICP-MS. The mining activity in As Pontes was found to have significantly impacted the aquatic ecosystem. Concentrations of Cd, Ni, Co, and Cu in the livers of the lake fish were significantly higher than in the livers of the other groups of fish, probably due to run-off from the spoil heap reaching the lake. Similarly, higher concentrations of Al, Pb, and Hg were observed in the river trout, associated with coal combustion at a near thermal power plant. Despite these variations, toxic and essential elements in both the pit lake and river remained low, within water quality standards, and do not pose an immediate threat to aquatic life. Concentrations of the metals in the muscle of wild fish, although higher than in farmed fish, were within EU limits. The study findings highlight the value of fish as indicators of environmental exposure and emphasize the ongoing need to monitor the pit lake to assess the long-term impacts of mining activities on aquatic health. Examination of liver tissue proved to be particularly useful in the assessments, providing a precise reflection of metal exposure and serving as a valuable means of evaluating environmental contamination and ecosystem health.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"374 ","pages":"Article 144212"},"PeriodicalIF":8.1,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}