Proceedings of the 7th World Congress on Recent Advances in Nanotechnology最新文献

{"title":"Study of Chitosan-Arginine with Different Degrees of Substitution at Chitosomes Nanoparticles Formulation for Gene Delivery","authors":"B. Garcia, O. Mertins, S. Han","doi":"10.11159/nddte22.146","DOIUrl":"https://doi.org/10.11159/nddte22.146","url":null,"abstract":"Development of efficient and safe gene delivery systems is still a big challenge for human and animal gene therapy. Nanoparticles are safer than viral vectors for gene delivery in terms of virus-related concerns, but transfection efficiency and toxicity of nanocarriers still need to be improved for clinical applications. Many categories of nanoparticles have been synthesized based on the physicochemical properties of building-blocks. Chitosan is derived from the chitin, and it is already available for medical and biomedical purposes, and liposomes are largely used for drug and gene delivery.[1] Therefore, in general, these materials are considered biocompatible, biodegradable, less toxic and immunogenic. As these nanoparticles have distinct physicochemical properties, a new category of nanoparticle was created complexing chitosan and liposome: chitosome. Recently, we synthesized chitosomes with DOPE/DOTAP surfactants and arginine-modified chitosan (CH-Arg) which showed a very high transfection rate in HEK293 cells (86% ± 3).[2] In this study we investigated the effect of CH-Arg with different degrees of substitution (DS), in chitosome formulation and their interaction with plasmids. The CH-Arg with low and high DS were synthesized by a method previously described,[3] and chitosomes were synthesized by the association of DOPE/DOTAP (L) and CH-Arg High or Low by reverse phase evaporation technique. Elemental analysis, fourier transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (H1-NMR) spectroscopies were performed for CH-Arg characterization. Dynamic light scattering (DLS) and zeta potential (ZP) were performed for chitosome characterization and measures were made periodically for 1 year to assess the aggregation and sedimentation behaviours after storing it at 25ºC and 4ºC. Chitosomes were complexed with the plasmid pEGFP-N3 and in vitro transfection efficacy was assessed by the fluorescent green protein (GFP) expression in HeLa cells by fluorescence microscopy.","PeriodicalId":276715,"journal":{"name":"Proceedings of the 7th World Congress on Recent Advances in Nanotechnology","volume":"56 35","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132389766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statistical Distribution of Charge Carriers in β-HgS Quantized Layer in Lateral Electrostatic Field","authors":"V. Harutyunyan","doi":"10.11159/icnnfc22.151","DOIUrl":"https://doi.org/10.11159/icnnfc22.151","url":null,"abstract":"- The specificity of the statistical distribution of electrons, heavy and light holes in a plane-parallel metacinnabar film under conditions of a quantum size effect and in the presence of a transversal electrostatic field is considered in the effective mass approximation. Consideration was carried out for the case when only the first film subband of size quantization is filled.Explicit analytical expressions are obtained for the chemical potential and concentration, internal energy, and heat capacity of the electron-hole subsystem in the quantized filmin the absence and presence of an external field. The analysis of the dependence of the indicated statistical characteristics of the material on the quantizing thickness of the layer, the strength of the external fieldand temperature of the system is carried out. The corresponding numerical estimates are also given.","PeriodicalId":276715,"journal":{"name":"Proceedings of the 7th World Congress on Recent Advances in Nanotechnology","volume":"68 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130328873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}