ACS Synthetic Biology最新文献

{"title":"","authors":"Sen Yang, Wen-Yue Tong, Chao-Hao Guo, Nan Shi, Xiao-Yun Liu* and Ming Kang*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Annika Grieß-Osowski, Madalen Robert, Moni Qiande, Stefanie Clauss and Cătălin Voiniciuc*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00181","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Ivana Cengic,  and , Elton P. Hudson*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00320","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Wei Wang, Nan Ma, Tian Xu, Yi-Lin Wu, Guo-Song Zheng, Li-Sha Zou, Xian-Lin Niu, Jin Yang, Guo-Ping Zhao, Yin-Hua Lu* and Hua Yuan*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Membrane Vesicle Production via <i>oprF</i> or <i>oprI</i> Deletion Has Distinct Phenotypic Effects in <i>Pseudomonas putida</i>.","authors":"Rebecca A Wilkes, Tarryn E Miller, Jacob Waldbauer, Nanqing Zhou, Lichun Zhang, Beth N DiBiase, Neha P Kamat, Ludmilla Aristilde, Gregg T Beckham, Allison Z Werner","doi":"10.1021/acssynbio.5c00171","DOIUrl":"10.1021/acssynbio.5c00171","url":null,"abstract":"<p><p>Membrane vesicle (MV) production is a natural phenomenon in Gram-negative bacteria and represents an emerging synthetic biology tool for the secretion of biomolecules or bioproducts. Manipulation of membrane components has proven successful in enhancing MV production. However, the impact of membrane disruptions on strain fitness and protein composition warrants further investigation for the use of MVs in industrial bioprocesses. Here, we identify and characterize two genetic engineering strategies for inducing hypervesiculation─deletion of genes for the outer membrane porin OprF or the lipoprotein OprI─in the commonly used platform <i>Pseudomonas putida</i> KT2440. Deletion of <i>oprI</i> generated up to a 1.5-fold increase in MVs, larger MVs with a greater proportion of outer membrane proteins, and no significant impact on strain fitness compared to wild type. In contrast, deletion of <i>oprF,</i> relative to wild type, generated up to a 4-fold increase in MVs but diminished growth, permeabilized membranes, and increased cytosolic protein packaging. Both hypervesiculation phenotypes increased nontargeted and MV-targeted mNeonGreen extracellular signal by up to 6-fold, demonstrating vesiculation as a mechanism for protein secretion. Despite increased blebbing of MVs from gene deletions, proteins involved in membrane biosynthesis were not elevated relative to wild type. Overexpression of <i>gpsA,</i> which initiates glycerophospholipid biosynthesis, in the Δ<i>oprF</i> background improved the membrane integrity by 37% and maintained MV formation, highlighting the importance of membrane biosynthesis in restoring the membrane in hypervesiculating strains. Together, this study provides genetic engineering strategies with corresponding phenotypic outcomes toward providing a synthetic biology toolset for MV deployment in <i>P. putida</i>.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2739-2752"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Small RNAs as Synthetic Post-transcriptional Regulators in Bacteria.","authors":"Jens Georg, Bork A Berghoff, Daniel Schindler","doi":"10.1021/acssynbio.5c00118","DOIUrl":"10.1021/acssynbio.5c00118","url":null,"abstract":"<p><p>Bacteria can respond to environmental changes by expressing small RNAs (sRNAs), which regulate mRNAs by complementary base-pairing. This regulatory mechanism allows bacteria to rapidly adapt their proteome. In recent years, sRNAs have gained attention as blueprints for synthetic regulators allowing control over user-defined targets. Multiple aspects need to be considered for efficient application of these versatile, on-demand, and easy-to-use tools. Advances in computational prediction and bioengineering concepts are the dawn of systematic synthetic sRNA biology. We provide an overview of sRNAs and alternative post-transcriptional regulators, highlight the requirements for successful regulation, and provide guidelines for design, construction, and sRNA application.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2405-2417"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illuminating Glucomannan Synthases To Explore Cell Wall Synthesis Bottlenecks.","authors":"Annika Grieß-Osowski, Madalen Robert, Moni Qiande, Stefanie Clauss, Cătălin Voiniciuc","doi":"10.1021/acssynbio.5c00181","DOIUrl":"10.1021/acssynbio.5c00181","url":null,"abstract":"<p><p>Hemicelluloses are important dietary fibers and a key component of lignocellulosic biomass. Despite numerous observations for fluorescently tagged cellulose synthases, the subcellular journeys and biochemical activities of intracellular cellulose synthase-like enzymes such as β-mannan synthases (ManS) remain largely unexplored. This study identifies C-terminal fluorescent protein tags that maintain ManS activity in yeast to accelerate the Design, Build, Test, Learn cycles for polysaccharide biosynthesis. Using the <i>Amorphophallus konjac</i> ManS as a case study, we demonstrate that the enzyme colocalizes with a known yeast marker for the Golgi apparatus despite the toxic effects of plant glucomannan accumulation in <i>Pichia pastoris</i>. The ManS first transmembrane domain was found to be critical for the punctate localization of the enzyme, its overall expression level and its function. Additionally, we explored how fluorescently tagged ManS is influenced by genetic or chemical perturbations of native yeast cell wall components, such as reducing protein mannosylation and severely disrupting β-1,3-glucans. Finally, we identified alternative feeding strategies and episomal vectors for <i>Pichia</i>, which were extended to <i>Saccharomyces cerevisiae</i>, to accelerate hemicellulose research. We propose that expanding the Plant MoClo-compatible plasmid repertoire is essential to swiftly prototype carbohydrate-active enzymes in yeast before proceeding with more time-intensive analyses in plants. Requiring only hours or days instead of weeks or months for plant transformation/regeneration, our yeast prototyping strategies can derisk the bioengineering of carbohydrate-active enzymes.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2472-2479"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12281619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Dominik L. Siebert,&nbsp;Frank Sargent,&nbsp;Ammar Al-Shameri* and Volker Sieber*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00150","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Rebecca A. Wilkes,&nbsp;Tarryn E. Miller,&nbsp;Jacob Waldbauer,&nbsp;Nanqing Zhou,&nbsp;Lichun Zhang,&nbsp;Beth N. DiBiase,&nbsp;Neha P. Kamat,&nbsp;Ludmilla Aristilde,&nbsp;Gregg T. Beckham and Allison Z. Werner*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00171","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144646082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directed Evolution of a SelB Variant that Does Not Require a Selenocysteine Insertion Sequence Element for Function.","authors":"Satoshi Ishida, Arno Gundlach, Clayton W Kosonocky, Andrew D Ellington","doi":"10.1021/acssynbio.5c00106","DOIUrl":"10.1021/acssynbio.5c00106","url":null,"abstract":"<p><p>In bacteria, the incorporation of selenocysteine is achieved through the interaction of the selenocysteine specific elongation factor (SelB) with selenocysteine-charged tRNA^Sec and a selenocysteine insertion sequence (SECIS) element adjacent to an opal stop codon in an mRNA. The more generalized, SECIS-independent incorporation of selenocysteine is of interest because of the high nucleophilicity of selenium and the greater durability of diselenide bonds. It is likely that during the course of evolution, selenocysteine insertion originally arose without the presence of a SECIS element, relying only on SelB. Herein, we undertake experiments to evolve an ancestral version of SelB that is SECIS-independent and show that not only can this protein (SelB-v2) generally incorporate selenocysteine across from stop codons but also that the new, orthogonal translation factor can be repurposed to other amino acids, such as serine. Given the delicate energetic balancing act already performed by EF-Tu, this achievement raises the possibility that greatly expanded genetic codes that relied in part on SelB-based loading can now be contrived.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2681-2689"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}