发布求助
文献互助 智能选刊 最新文献

ACS Synthetic Biology最新文献

筛选
英文 中文
De Novo Synthesis of Tyrosol and Hydroxytyrosol through Temperature-Inducible Systems and Metabolic Engineering. 利用温度诱导系统和代谢工程从头合成酪醇和羟基酪醇。
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20 Epub Date: 2025-05-23 DOI: 10.1021/acssynbio.5c00172
Xiaochuan Chen, Tao Qian, Wenping Wei, Yihui Zhu, Gaopan Cai, Mengfan Li, Xiaohe Chu, Bang-Ce Ye
{"title":"<i>De Novo</i> Synthesis of Tyrosol and Hydroxytyrosol through Temperature-Inducible Systems and Metabolic Engineering.","authors":"Xiaochuan Chen, Tao Qian, Wenping Wei, Yihui Zhu, Gaopan Cai, Mengfan Li, Xiaohe Chu, Bang-Ce Ye","doi":"10.1021/acssynbio.5c00172","DOIUrl":"10.1021/acssynbio.5c00172","url":null,"abstract":"<p><p>Hydroxytyrosol (HT) has various biological and pharmacological activities, including potent antioxidant activity. The efficient synthesis of HT and tyrosol has been achieved by microbial synthesis. However, more strategies are needed to enhance its yield and meet the demands of industrialization. In this study, <i>SceARO10</i> and <i>EcoyahK</i> were used for the <i>de novo</i> synthesis of tyrosol in <i>Escherichia coli</i> using a temperature-inducible system. Different sources of phenolic acid decarboxylase and alcohol reductase were investigated, with <i>YliARO10</i> and <i>YliPAR4</i> from <i>Yarrowia lipolytica</i> showing the best catalytic performance, yielding 4.05 g/L of tyrosol at 60 h in shake flasks, the highest yield reported. Next, <i>EblHpaBC</i> from <i>E. coli</i> BL21 (DE3) was introduced for HT biosynthesis, and the HT-related degradation gene <i>mhpB</i> was functionally characterized in <i>E. coli</i>. Subsequently, by enhancing precursor supply, eliminating competing metabolic pathways, and knocking out <i>mhpB</i>, the HT yield reached 1.28 g/L after 60 h. Finally, in a 5 L bioreactor, titers of 6.18 and 4.97 g/L of tyrosol and HT were achieved for the first time using a temperature-induced strategy. This study presents a method for the modification of microbial chassis for the efficient synthesis of tyrosol and HT.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2294-2304"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimized Phage Display-Based Selection for the Development of Heterodimerizing Optogenetic Tools. 基于噬菌体展示的优化选择开发异二聚化光遗传工具。
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20 Epub Date: 2025-05-08 DOI: 10.1021/acssynbio.5c00167
Giang N T Le, Jaewan Jang, Maruti Uppalapati, G Andrew Woolley
{"title":"Optimized Phage Display-Based Selection for the Development of Heterodimerizing Optogenetic Tools.","authors":"Giang N T Le, Jaewan Jang, Maruti Uppalapati, G Andrew Woolley","doi":"10.1021/acssynbio.5c00167","DOIUrl":"10.1021/acssynbio.5c00167","url":null,"abstract":"<p><p>Multiple display techniques, including phage display, mRNA display, and ribosome display, have been used to expand the optogenetic toolbox beyond what nature provides. These techniques are most often applied to the development of binding partners that selectively recognize different conformational states of photoswitchable proteins. However, for some targets, in particular the spectrally diverse cyanobacteriochrome (CBCR) GAF domain family, the subtle differences between conformational states pose a significant challenge to discovering highly selective binders. We present an optimized phage display-based protocol designed to effectively capture these subtle changes. This optimized protocol applies high selection pressure by changing the elution method and tightening negative selection, leading to the enrichment of selective binders. Through multiple selection campaigns, we demonstrate the utility of this protocol for identifying highly selective binders.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2400-2404"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
Juhyun Kim, Alexander P.S. Darlington*, Said Muñoz-Montero, Rafael Montenegro, Perrine Dalby, Noemí Herrera-Martín, Alice Banks, Satya Prakash, Karen Polizzi, Declan G. Bates and José I. Jiménez*, 
{"title":"","authors":"Juhyun Kim,&nbsp;Alexander P.S. Darlington*,&nbsp;Said Muñoz-Montero,&nbsp;Rafael Montenegro,&nbsp;Perrine Dalby,&nbsp;Noemí Herrera-Martín,&nbsp;Alice Banks,&nbsp;Satya Prakash,&nbsp;Karen Polizzi,&nbsp;Declan G. Bates and José I. Jiménez*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.4c00828","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
Rhudith B. Cabulong, Won-Gyun Oh, Tahseena Naaz and Beom Soo Kim*, 
{"title":"","authors":"Rhudith B. Cabulong,&nbsp;Won-Gyun Oh,&nbsp;Tahseena Naaz and Beom Soo Kim*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.4c00880","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/sbv014i006_1949731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
Sara Baldanta,  and , Guillermo Rodrigo*, 
{"title":"","authors":"Sara Baldanta,&nbsp; and ,&nbsp;Guillermo Rodrigo*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
Khushank Singhal, Harry E. Adamson, Thomas M. Baer, Howard M. Salis and Melik C. Demirel*, 
{"title":"","authors":"Khushank Singhal,&nbsp;Harry E. Adamson,&nbsp;Thomas M. Baer,&nbsp;Howard M. Salis and Melik C. Demirel*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.5c00205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20
Tessema K. Kassaw, Wenlong Xu, Christopher S. Zalewski, Katherine Kiwimagi, Ron Weiss, Mauricio S. Antunes, Ashok Prasad* and June I. Medford*, 
{"title":"","authors":"Tessema K. Kassaw,&nbsp;Wenlong Xu,&nbsp;Christopher S. Zalewski,&nbsp;Katherine Kiwimagi,&nbsp;Ron Weiss,&nbsp;Mauricio S. Antunes,&nbsp;Ashok Prasad* and June I. Medford*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssynbio.4c00777","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144429611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication, Evolution, and Mutual Conversion of d-Fucose-Activatable and -Repressible Acetyltransferase upon Mutations. d-聚焦可激活和可抑制乙酰转移酶在突变中的制造、进化和相互转化。
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20 Epub Date: 2025-04-30 DOI: 10.1021/acssynbio.4c00169
Yuki Yanai, Miyu Tsukada, Yuki Kimura, Daisuke Umeno
{"title":"Fabrication, Evolution, and Mutual Conversion of d-Fucose-Activatable and -Repressible Acetyltransferase upon Mutations.","authors":"Yuki Yanai, Miyu Tsukada, Yuki Kimura, Daisuke Umeno","doi":"10.1021/acssynbio.4c00169","DOIUrl":"10.1021/acssynbio.4c00169","url":null,"abstract":"<p><p>The fusion of different proteins can result in the linkage-dependent emergence of molecular switches. We inserted chloramphenicol acetyltransferase (CAT) from <i>Escherichia coli</i> into a loop of a d-fucose-responsive mutant of transcription factor AraC, using linker libraries with various lengths. We found that on-switches tend to emerge when two proteins are fused with linkers long enough to fill the gap of the distance of residues to be connected, while fusing with short or zero linkers results in the frequent emergence of off-switches. Both types of switches rapidly evolved their switching efficiency upon mutations, establishing the d-fucose-on and -off regulations of CAT activity without disrupting the d-fucose-inducible logic of AraC function. To our surprise, both one-input/two-output split gates thus obtained could be easily interconverted upon mutations. Through mutations, fusion proteins rapidly establish and evolve mutual regulatory relationships with unrelated partner proteins, enabling diverse functional outcomes. Furthermore, random mutagenesis can alter the behavior of these emergent regulatory relationships, such as interconverting the activation or deactivation of the partner protein upon ligand binding, sometimes at a surprisingly high frequency.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"1936-1947"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of a High-Throughput Screen for Monitoring Disease-Associated Protein Misfolding and Aggregation in Bacteria. 细菌中疾病相关蛋白错误折叠和聚集的高通量筛选优化
IF 3.7 2区 生物学
ACS Synthetic Biology Pub Date : 2025-06-20 Epub Date: 2025-05-12 DOI: 10.1021/acssynbio.5c00166
Dafni C Delivoria, Eleni Konia, Ilias Matis, Georgios Skretas
{"title":"Optimization of a High-Throughput Screen for Monitoring Disease-Associated Protein Misfolding and Aggregation in Bacteria.","authors":"Dafni C Delivoria, Eleni Konia, Ilias Matis, Georgios Skretas","doi":"10.1021/acssynbio.5c00166","DOIUrl":"10.1021/acssynbio.5c00166","url":null,"abstract":"<p><p>Protein misfolding and aggregation are central features of a wide range of diseases, including neurodegenerative disorders, systemic amyloidoses, and cancer. The identification of compounds that can modulate protein folding and aggregation is a key step toward developing effective therapies. High-throughput screening methods are essential for efficiently identifying such compounds. In this study, we optimized a previously developed high-throughput genetic screen for monitoring protein misfolding and aggregation in bacteria. This system is based on monitoring the fluorescence of <i>Escherichia coli</i> cells expressing fusions of human misfolding-prone and disease-related proteins (MisPs) with the green fluorescent protein. We systematically tested a variety of experimental conditions, such as overexpression conditions and MisP-GFP fusion formats, to identify key parameters that affect the sensitivity and dynamic range of the assay. Using misfolding-prone, cancer-associated variants of human p53 as a model system, we found that strong overexpression conditions, such as high copy number vectors, strong promoters, high inducer concentrations, and high overexpression temperatures, can yield optimal assay performance. These optimized assay conditions were also validated with additional MisPs, such as the Alzheimer's disease-associated amyloid-β peptide and variants of superoxide dismutase 1 associated with amyotrophic lateral sclerosis. At the same time, we observed that certain conditions, such as inducer concentrations and overexpression temperature, may need to be precisely fine-tuned for each new MisP target to yield optimal assay performance. Our findings provide a framework for standardizing MisP-GFP screening assays, facilitating their broad application in the discovery of therapeutic agents targeting protein misfolding and aggregation.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2283-2293"},"PeriodicalIF":3.7,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：604180095
Book学术官方微信