ACS Synthetic Biology最新文献_第10页

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Davide Salzano, Barbara Shannon, Claire Grierson, Lucia Marucci, Nigel J. Savery and Mario di Bernardo*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Yizhou Luo, Lihao Fu, Jueru Chen, Hongrong Xu, Zeqi Song, Khurshid Jalal, Yongcan Chen, Wenhao Xie, Shujun Tian, Xiaoting Fang, Tong Si* and Jianzhi Zhang*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Yuchen Han, Huayi Gao*, Shuo Wang, Haiyan Ju, Ran Ge, Chaoyou Xue, Weidong Liu, Xiaolong Jiang* and Wuyuan Zhang*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Mackenzie Thornbury, Adrien Knoops, Iain Summerby-Murray, James Dhaliwal, Sydney Johnson, Joseph Christian Utomo, Jaya Joshi, Lauren Narcross, Gabriel Remondetto, Michel Pouliot, Malcolm Whiteway and Vincent J. J. Martin*,

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Engineering Temperature-Powered Synthetic Multilayer Molecular Bioswitch for High-Level Pyruvate Derivative Production in Escherichia coli. 工程温度驱动的合成多层分子生物开关在大肠杆菌中生产高水平丙酮酸衍生物。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-17 DOI: 10.1021/acssynbio.5c00269

Yang Li, Mingxiong Liu, Changyang Yang, Yujia Zheng, Guiping Xu, Hongxin Fu, Jufang Wang

{"title":"Engineering Temperature-Powered Synthetic Multilayer Molecular Bioswitch for High-Level Pyruvate Derivative Production in Escherichia coli.","authors":"Yang Li, Mingxiong Liu, Changyang Yang, Yujia Zheng, Guiping Xu, Hongxin Fu, Jufang Wang","doi":"10.1021/acssynbio.5c00269","DOIUrl":"https://doi.org/10.1021/acssynbio.5c00269","url":null,"abstract":"Microbial engineering and genetically encoded bioswitches provide an excellent platform for the production of high-value biochemicals. However, the synthesis of biobased compounds associated with central pathways or multiple bypasses is often restricted by robust central metabolism, incompatible metabolic homeostasis, and limited genetic tools. Here, a programmable and bifunctional temperature- and pyruvate-responsive biosensor was designed to establish an efficient biosynthesis platform for pyruvate derivatives. Especially, the novel variant L185P of regulator CI857 exhibited a 37.08% improvement in thermostability by forming a ring oscillation and a more rigid structure. Subsequently, the tool TPLC, based on thermosensor- and pyruvate biosensor-based layered circuit, was created. Using iso-butylamine synthesis as a proof-of-concept, a 3.31-fold increase in titer was achieved by precisely fine-tuning cell metabolism and bioproduct synthesis, and its titer further reached 46.24 g/L in a fermenter. This work highlights powerful toolkits for regulating multigene pathways, enabling the efficient production of high-value bioproducts of industrial interest of industry.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Yarrowia lipolytica with Massive Gene Assembly and Genomic Integration. 利用大量基因组装和基因组整合的多脂耶氏菌代谢工程。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-17 DOI: 10.1021/acssynbio.5c00333

Yuhui Cui, Duo Liu, Huimin Xue, Mingshan Li, Wenhong Guo, Cuiqin Huang, Xintian Zheng, Jichao Yang, Hong Liu, Huifang Yin, Hanjie Wang

{"title":"Metabolic Engineering of Yarrowia lipolytica with Massive Gene Assembly and Genomic Integration.","authors":"Yuhui Cui, Duo Liu, Huimin Xue, Mingshan Li, Wenhong Guo, Cuiqin Huang, Xintian Zheng, Jichao Yang, Hong Liu, Huifang Yin, Hanjie Wang","doi":"10.1021/acssynbio.5c00333","DOIUrl":"https://doi.org/10.1021/acssynbio.5c00333","url":null,"abstract":"Synthetic biology has developed varied strategies of one-pot multigene assembly and genomic integration to promote the genetic engineering of the chassis. However, such strategies for engineering oleaginous yeast Yarrowia lipolytica is lacking, given the current stage that at most 5 exogenous genes (around 13 kb) can be assembled and integrated into the genome at once. Here, we developed a strategy of massive gene assembly and integration in the Y. lipolytica genome. As a proof of concept, dozen-gene assembly (more than 30 kb) and integration were achieved stably and reproducibly, and a Y. lipolytica chassis containing a total of 35 exogenous genes (a sum of 93.5 kb) was constructed. The introduction of massive genes modulated the synthesis of lycopene, a heterologous natural product, to quite different extents. Ultimately, an optimized constructed strain containing 15 exogenous genes achieved the highest yield of 144.58 mg/g DCW and produced a lycopene titer of 2144.83 mg/L in a 5 L bioreactor. Our strategy significantly expands the capability of Y. lipolytica genetic manipulation and metabolic engineering.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144657842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Progress in the Biosynthesis of Cosmetic Ingredients through Engineering of Saccharomyces cerevisiae. 酿酒酵母工程生物合成化妆品原料的研究进展。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-16 DOI: 10.1021/acssynbio.5c00199

Zhongjuan Di, Yanjun Huo, Guan Wang, Yingping Zhuang

{"title":"Progress in the Biosynthesis of Cosmetic Ingredients through Engineering of Saccharomyces cerevisiae.","authors":"Zhongjuan Di, Yanjun Huo, Guan Wang, Yingping Zhuang","doi":"10.1021/acssynbio.5c00199","DOIUrl":"https://doi.org/10.1021/acssynbio.5c00199","url":null,"abstract":"Driven by consumer preferences for safety and environmental protection, the global cosmetics industry has an increasing demand for natural and sustainable ingredients. Saccharomyces cerevisiae has emerged as a powerful platform for the biosynthesis of cosmetic ingredients due to its strong metabolic capacity, genetic operability, and cost-effective production capabilities. This review focuses on the latest advances in S. cerevisiae for the production of high-value cosmetic compounds, including antioxidants, repair agents, moisturizers, and structure-maintaining ingredients. Key strategies, such as genetic and metabolic engineering, pathway modularity, and fermentation optimization, are discussed, demonstrating significant improvements in yield and efficiency. In addition, the integration of artificial intelligence and machine learning in strain design and process control is explored, providing promising solutions to overcome metabolic bottlenecks and scale up production. Despite challenges such as metabolic burden, S. cerevisiae shows great potential for sustainable and scalable biosynthesis of cosmetic ingredients, paving the way for the next generation of biobased cosmetics. This comprehensive review provides valuable insights and technical references for the development of the field of synthetic biology in the cosmetics industry.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144647956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Control for High-Efficiency Ectoine Synthesis in Engineered Escherichia coli. 工程大肠杆菌高效合成艾克托因的代谢控制。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-15 DOI: 10.1021/acssynbio.5c00330

Zheng Lei, Xiangsong Chen, Lixia Yuan, Jinyong Wu, Jianming Yao

{"title":"Metabolic Control for High-Efficiency Ectoine Synthesis in Engineered Escherichia coli.","authors":"Zheng Lei, Xiangsong Chen, Lixia Yuan, Jinyong Wu, Jianming Yao","doi":"10.1021/acssynbio.5c00330","DOIUrl":"https://doi.org/10.1021/acssynbio.5c00330","url":null,"abstract":"Ectoine is a pivotal natural osmoprotectant that functions as a compatible solute through osmoregulation, enabling microorganisms to thrive in extreme environments such as high salinity. To meet market demands, this study focuses on optimizing its production process. We initially engineered the ectABC gene cluster from Halomonas venusta via 5'-UTR modification, establishing a functional ectoine biosynthesis pathway in E. coli. Subsequent introduction of a rate-limiting enzyme EctB mutant (E407D) and aspartokinase mutant increased titer by 140%. To address lysine byproduct accumulation, an innovative molecular switch was employed to regulate lysA gene expression, achieving dynamic balance between cell growth and product synthesis. Further optimization through cofactor engineering yielded the final strain ECT31, which produced 164.6 g/L ectoine in a 100 L bioreactor within 117 h, the highest reported titer for E. coli-based ectoine production to date. The metabolic engineering strategy presented herein establishes a new pdigm for efficient biosynthesis of amino acid derivatives.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Directed Evolution for the Discovery of Engineered Proteins and Small Peptides Using Molecular Mutagenesis. 利用分子诱变发现工程蛋白和小肽的定向进化。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-14 DOI: 10.1021/acssynbio.4c00887

Yi Torng Chai, Chee Mun Fang, Yin Sze Lim, Hwei-San Loh, Cheng Foh Le

{"title":"Directed Evolution for the Discovery of Engineered Proteins and Small Peptides Using Molecular Mutagenesis.","authors":"Yi Torng Chai, Chee Mun Fang, Yin Sze Lim, Hwei-San Loh, Cheng Foh Le","doi":"10.1021/acssynbio.4c00887","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00887","url":null,"abstract":"Random mutagenesis is an essential technique in the directed evolution of proteins and peptides, driving advancements in protein engineering and biotechnology. This review provides a critical analysis of various error-prone polymerase chain reaction (epPCR) techniques employed for random mutagenesis, highlighting their mechanisms, advantages, and limitations. We compare conventional methods with emerging approaches, including combinative techniques and specialized protocols for small amplicons. We also discuss a few alternative approaches for cloning a mutant gene library, which could be simpler and more efficient than the traditional restriction digestion-ligation method, significantly improving the directed evolution workflows. Ultimately, the selection of a suitable method should align with the specific goals of the research, accepting inherent trade-offs. By combining different mutagenesis techniques with complementary mutational spectra, researchers can optimize their strategies for the discovery of novel proteins and peptides with specific biological activities and physicochemical properties of interest.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Engineering of Methanotrophic Bacteria for De Novo Production of Taxadiene from Methane. 甲烷营养菌从甲烷中重新生成杉二烯的代谢工程研究。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-14 DOI: 10.1021/acssynbio.5c00109

Xinzhe Zhang, Aipeng Li, Xiaohan Huang, Shuqi Guo, Chenyue Zhang, Ramon Gonzalez, Qiang Fei

{"title":"Metabolic Engineering of Methanotrophic Bacteria for De Novo Production of Taxadiene from Methane.","authors":"Xinzhe Zhang, Aipeng Li, Xiaohan Huang, Shuqi Guo, Chenyue Zhang, Ramon Gonzalez, Qiang Fei","doi":"10.1021/acssynbio.5c00109","DOIUrl":"https://doi.org/10.1021/acssynbio.5c00109","url":null,"abstract":"The growing demand for natural products in pharmaceutical applications has prompted a focus on more sustainable methods to produce high-value terpenoids using microbial cell factories. Due to the characteristics of high abundance, renewable, and low cost, methane has emerged as a promising feedstock for biomanufacturing. In this study, the methanotrophic bacterium Methylotuvimicrobium buryatenese 5GB1C, known for its industrial potential, was metabolically engineered to synthesize taxadiene, a crucial precursor in paclitaxel production. The biosynthesis of taxadiene from methane was first established by employing an endogenous strong promoter to enhance the expression of heterologous taxadiene synthase in M. buryatenese 5GB1C. To further optimize the metabolic flux, rate-limiting enzymes (Dxs and IspA) were upregulated in the methylerythritol phosphate pathway while complementing the native pathway with the essential idi gene that was originally deficient. Coupled with a 2.1-fold improvement in the NADPH/NADP+ ratio, these modifications collectively boosted taxadiene production from 2.58 to 22.97 mg/L in serum vials. Ultimately, a temperature-controlled two-stage cultivation was implemented in 3 L bioreactors, which achieved a remarkable titer of 104.88 mg/L, representing the highest reported titer for diterpenoid biosynthesis from methane. This work demonstrates the potential of utilizing methane for the sustainable production of advanced terpenoids with reduced environmental impact.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144624968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0