ACS Synthetic Biology最新文献_第10页

Semiautomated Production of Cell-Free Biosensors

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-12 DOI: 10.1021/acssynbio.4c0070310.1021/acssynbio.4c00703

Dylan M. Brown, Daniel A. Phillips, David C. Garcia, Anibal Arce, Tyler Lucci, John P. Davies Jr., Jacob T. Mangini, Katherine A. Rhea, Casey B. Bernhards, John R. Biondo, Steven M. Blum, Stephanie D. Cole, Jennifer A. Lee, Marilyn S. Lee, Nathan D. McDonald, Brenda Wang, Dale L. Perdue, Xavier S. Bower, Walter Thavarajah, Ashty S. Karim, Matthew W. Lux, Michael C. Jewett, Aleksandr E. Miklos* and Julius B. Lucks*,

{"title":"Semiautomated Production of Cell-Free Biosensors","authors":"Dylan M. Brown, Daniel A. Phillips, David C. Garcia, Anibal Arce, Tyler Lucci, John P. Davies Jr., Jacob T. Mangini, Katherine A. Rhea, Casey B. Bernhards, John R. Biondo, Steven M. Blum, Stephanie D. Cole, Jennifer A. Lee, Marilyn S. Lee, Nathan D. McDonald, Brenda Wang, Dale L. Perdue, Xavier S. Bower, Walter Thavarajah, Ashty S. Karim, Matthew W. Lux, Michael C. Jewett, Aleksandr E. Miklos* and Julius B. Lucks*, ","doi":"10.1021/acssynbio.4c0070310.1021/acssynbio.4c00703","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00703https://doi.org/10.1021/acssynbio.4c00703","url":null,"abstract":"Cell-free synthetic biology biosensors have potential as effective in vitro diagnostic technologies for the detection of chemical compounds, such as toxins and human health biomarkers. They have several advantages over conventional laboratory-based diagnostic approaches, including the ability to be assembled, freeze-dried, distributed, and then used at the point of need. This makes them an attractive platform for cheap and rapid chemical detection across the globe. Though promising, a major challenge is scaling up biosensor manufacturing to meet the needs of their multiple uses. Currently, cell-free biosensor assembly during lab-scale development is mostly performed manually by the operator, leading to quality control and performance variability issues. Here we explore the use of liquid-handling robotics to manufacture cell-free biosensor reactions. We compare both manual and semiautomated reaction assembly approaches using the Opentrons OT-2 liquid handling platform on two different cell-free gene expression assay systems that constitutively produce colorimetric (LacZ) or fluorescent (GFP) signals. We test the designed protocol by constructing an entire 384-well plate of fluoride-sensing cell-free biosensors and demonstrate that they perform close to expected detection outcomes.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"979–986 979–986"},"PeriodicalIF":3.7,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic Biology Strategies for the Production of Natural Colorants and Their Non-Natural Derivatives

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-11 DOI: 10.1021/acssynbio.4c0079910.1021/acssynbio.4c00799

Kyoungwon Kim, Kyubin Shim, Ying Wei Wang and Dongsoo Yang*,

{"title":"Synthetic Biology Strategies for the Production of Natural Colorants and Their Non-Natural Derivatives","authors":"Kyoungwon Kim, Kyubin Shim, Ying Wei Wang and Dongsoo Yang*, ","doi":"10.1021/acssynbio.4c0079910.1021/acssynbio.4c00799","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00799https://doi.org/10.1021/acssynbio.4c00799","url":null,"abstract":"Colorants are widely used in our daily lives to give colors to diverse chemicals and materials, including clothes, food, drugs, cosmetics, and paints. Although synthetic colorants derived from fossil fuels have been predominantly used due to their low cost, there is a growing need to replace them with natural alternatives. This shift is driven by increasing concerns over the climate crisis caused by excessive fossil fuel use, as well as health issues associated with the consumption of foods, beverages, and cosmetics containing petroleum-derived chemicals. In addition, many natural colorants show health-promoting properties such as antioxidant and antimicrobial activities. Despite such advantages, natural colorants could not be readily commercialized and distributed in the market due to their low stability, limited color spectrum, and low yields from natural resources. To this end, synthetic biology approaches have been developed to efficiently produce natural colorants from renewable resources with high yields. Strategies to diversify natural colorants to produce non-natural derivatives with enhanced properties and an expanded color spectrum have been also developed. In this Review, we discuss the recent synthetic biology strategies developed for enhancing the production of natural colorants and their non-natural derivatives, together with accompanying examples. Challenges ahead and future perspectives are also discussed.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"662–676 662–676"},"PeriodicalIF":3.7,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering Chromatin Regulation of Xylose Utilization in Budding Yeast Saccharomyces cerevisiae for Efficient Bioconversion

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-10 DOI: 10.1021/acssynbio.4c0073010.1021/acssynbio.4c00730

Wei-Bin Wang, Rui-Qi Tang, Bing Yuan, Yue Wang, Guo-Dong Liu, Dong-Min Li, Hong-Jia Zhang, Xin-Qing Zhao* and Feng-Wu Bai,

{"title":"Engineering Chromatin Regulation of Xylose Utilization in Budding Yeast Saccharomyces cerevisiae for Efficient Bioconversion","authors":"Wei-Bin Wang, Rui-Qi Tang, Bing Yuan, Yue Wang, Guo-Dong Liu, Dong-Min Li, Hong-Jia Zhang, Xin-Qing Zhao* and Feng-Wu Bai, ","doi":"10.1021/acssynbio.4c0073010.1021/acssynbio.4c00730","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00730https://doi.org/10.1021/acssynbio.4c00730","url":null,"abstract":"Utilization of xylose as a renewable carbon source has received constant interest. Considering that the structure and state of eukaryotic chromatin are inextricably intertwined, it is significant to explore chromatin regulation for engineering xylose metabolism in yeast. Here, we show that two chromatin remodelers, namely, Swr1 and Isw1, affect xylose utilization in recombinant budding yeastSaccharomyces cerevisiae. Overexpressing SWR1 showed the highest increase in xylose utilization, up to 29.3%, compared to that of the parent strain. Furthermore, comparative transcriptome and chromatin immunoprecipitation sequencing (ChIP-seq) analyses revealed significantly different changes of gene expression by elevated expression of Swr1 and Isw1. Reduced histone H2A.Z occupancy in two key carbon-metabolism regulators of Mig2 and Sip2 was further observed in the engineered yeast. Further tests showed improved xylose utilization of the engineered yeast in the presence of corncob hydrolysate. Our results suggest that chromatin regulators are critical genetic elements in recombinant S. cerevisiae for engineering xylose metabolism.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"794–803 794–803"},"PeriodicalIF":3.7,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes 可溶性叶蝉蛋白的无细胞表达 Brochosomes

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-07 DOI: 10.1021/acssynbio.4c0077310.1021/acssynbio.4c00773

Caleb G. Lay, Gabriel R. Burks, Zheng Li, Jeffrey E. Barrick, Charles M. Schroeder, Ashty S. Karim and Michael C. Jewett*,

{"title":"Cell-Free Expression of Soluble Leafhopper Proteins from Brochosomes","authors":"Caleb G. Lay, Gabriel R. Burks, Zheng Li, Jeffrey E. Barrick, Charles M. Schroeder, Ashty S. Karim and Michael C. Jewett*, ","doi":"10.1021/acssynbio.4c0077310.1021/acssynbio.4c00773","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00773https://doi.org/10.1021/acssynbio.4c00773","url":null,"abstract":"Brochosomes are proteinaceous nanostructures produced by leafhopper insects with superhydrophobic and antireflective properties. Unfortunately, the production and study of brochosome-based materials has been limited by poor understanding of their major constituent subunit proteins, known as brochosomins, as well as their sensitivity to redox conditions due to essential disulfide bonds. Here, we used cell-free gene expression (CFE) to achieve recombinant production and analysis of brochosomin proteins. Through the optimization of redox environment, reaction temperature, and disulfide bond isomerase concentration, we achieved soluble brochosomin yields of up to 341 ± 30 μg/mL. Analysis using dynamic light scattering and transmission electron microscopy revealed distinct aggregation patterns among cell-free mixtures with different expressed brochosomins. We anticipate that the CFE methods developed here will accelerate the ability to change the geometries and properties of natural and modified brochosomes, as well as facilitate the expression and structural analysis of other poorly understood protein complexes.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"987–994 987–994"},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection 基于纳米抗体的侧流分析法用于快速检测寨卡病毒

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-07 DOI: 10.1021/acssynbio.4c0081910.1021/acssynbio.4c00819

Yuli Peng, Atheer Alqatari, Fabian Kiessling, Dominik Renn*, Raik Grünberg*, Stefan T. Arold* and Magnus Rueping*,

{"title":"Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection","authors":"Yuli Peng, Atheer Alqatari, Fabian Kiessling, Dominik Renn*, Raik Grünberg*, Stefan T. Arold* and Magnus Rueping*, ","doi":"10.1021/acssynbio.4c0081910.1021/acssynbio.4c00819","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00819https://doi.org/10.1021/acssynbio.4c00819","url":null,"abstract":"Zika virus infections remain severely underdiagnosed due to their initial mild clinical symptoms. However, recent outbreaks have revealed neurological complications in adults and severe deformities in newborns, emphasizing the critical need for accurate diagnosis. Lateral flow assays (LFAs) provide a rapid, cost-effective, and user-friendly method for antigen testing at point-of-care, bedside, or in home settings. LFAs utilizing nanobodies have multiple benefits over traditional antibody-based techniques, as nanobodies are much smaller, more stable, and simpler to manufacture. We introduce a nanobody-based LFA for the rapid identification of Zika virus antigens. Starting from two previously reported nanobodies recognizing the Zika nonstructural protein 1 (NS1), we evaluate periplasmic and cytosolic nanobody expression and test different purification tags and immobilization strategies. We quantify nanobody binding kinetics and validate their mutually noncompetitive binding. Avidity effects boost the capture of the tetrameric target protein by 3 orders of magnitude and point to a general strategy for higher sensitivity LFA sensing. The nanobody LFA detects Zika NS1 with a limit of detection ranging from 25 ng/mL in buffer to 1 ng/mL in urine. This nanobody-LFA has the potential to facilitate on-site and self-diagnosis, improve our understanding of Zika infection prevalence, and support public health initiatives in regions affected by Zika virus outbreaks.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"890–900 890–900"},"PeriodicalIF":3.7,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acssynbio.4c00819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Escherichia coli Surface Display: Advances and Applications in Biocatalysis

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-06 DOI: 10.1021/acssynbio.4c0079310.1021/acssynbio.4c00793

Wei Liu, Wenjun Sun*, CaiCe Liang, Tianpeng Chen, Wei Zhuang, Dong Liu, Yong Chen* and Hanjie Ying,

{"title":"Escherichia coli Surface Display: Advances and Applications in Biocatalysis","authors":"Wei Liu, Wenjun Sun*, CaiCe Liang, Tianpeng Chen, Wei Zhuang, Dong Liu, Yong Chen* and Hanjie Ying, ","doi":"10.1021/acssynbio.4c0079310.1021/acssynbio.4c00793","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00793https://doi.org/10.1021/acssynbio.4c00793","url":null,"abstract":"Escherichia coli surface display technology, which facilitates the stable display of target peptides and proteins on the bacterial surface through fusion with anchor proteins, has become a potent and versatile tool in biotechnology and biomedicine. The E. coli surface display strategy presents a unique alternative to classic intracellular and extracellular expression systems, facilitating the anchorage of target peptides and proteins on the cell surface for functional execution. This distinctive attribute also introduces a novel paradigm in the realm of biocatalysis, harnessing cells with surface-displayed enzymes to catalyze the conversion of substrates. This strategy effectively eliminates the requirement for enzyme purification, overcomes the limitations related to substrate transmembrane transport, improves enzyme activity and stability, and greatly reduces the cost of downstream product purification, thus making it widely used in biocatalysis. Here, we review recent advances in various surface display systems and surface display technology for biocatalytic applications. Additionally, we discuss the current limitations of this technology and several promising alternative display methods.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"648–661 648–661"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143666871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-06 DOI: 10.1021/acssynbio.4c0066510.1021/acssynbio.4c00665

Wenping Wei, Chengshuai Yang, Zhen Su, Yan Wang, Pingping Wang, Xing Yan* and Zhihua Zhou*,

{"title":"Engineering a Yeast Cell Factory to Sustainably Biosynthesize Parthenolide","authors":"Wenping Wei, Chengshuai Yang, Zhen Su, Yan Wang, Pingping Wang, Xing Yan* and Zhihua Zhou*, ","doi":"10.1021/acssynbio.4c0066510.1021/acssynbio.4c00665","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00665https://doi.org/10.1021/acssynbio.4c00665","url":null,"abstract":"The sesquiterpene lactone parthenolide is a promising anticancer drug. Its biosynthesis via a microbial cell factory has been considered as a sustainable alternative to plant extraction. Herein, systematic metabolic engineering approaches, as well as the introduction of a novel noncanonical tricarboxylic acid (TCA) cycle, were employed to enhance the production of the key precursor germacrene A. By identifying two new dehydrogenases and controlling the expression of parthenolide synthase, we further achieved the elimination of byproducts and enhanced parthenolide production. A two-stage fermentation approach and in situ product extraction using macroreticular resin were further applied to relieve the nocuous effect of costunolide and parthenolide on the growth of yeast cell factories, ultimately achieving a titer of 549.7 mg/L for parthenolide and 972.7 mg/L for costunolide in a 10 L fermenter, which represents the highest reported titer obtained by microbial fermentation. The strategies should also contribute to the microbial cell factory-construction for other natural products exhibiting toxicity.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"729–739 729–739"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing Biosensors for Specific Assessment of Trans-Translation in Pseudomonas aeruginosa

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-06 DOI: 10.1021/acssynbio.4c0080110.1021/acssynbio.4c00801

Bastien L’Hermitte, Thomas Chauvet, Sylvie Georgeault-Daguenet, Nicolas Le Yondre, Philippe Jehan, Reynald Gillet* and Christine Baysse*,

引用次数: 0

Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in Cupriavidus necator H16

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-06 DOI: 10.1021/acssynbio.4c0086910.1021/acssynbio.4c00869

Akira K. Nakamura, Emily M. Fulk, Christopher W. Johnson and Farren J. Isaacs*,

{"title":"Synthetic Genetic Elements Enable Rapid Characterization of Inorganic Carbon Uptake Systems in Cupriavidus necator H16","authors":"Akira K. Nakamura, Emily M. Fulk, Christopher W. Johnson and Farren J. Isaacs*, ","doi":"10.1021/acssynbio.4c0086910.1021/acssynbio.4c00869","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00869https://doi.org/10.1021/acssynbio.4c00869","url":null,"abstract":"Cupriavidus necator H16 is a facultative chemolithotroph capable of using CO2 as a carbon source, making it a promising organism for carbon-negative biomanufacturing of petroleum-based product alternatives. In contrast to model microbes, genetic engineering technologies are limited in C. necator, constraining its utility in basic and applied research. Here, we developed a genome engineering technology to efficiently mobilize, integrate, and express synthetic genetic elements (SGEs) in C. necator. We tested the chromosomal expression of four inducible promoters to optimize an engineered genetic landing pad for tunable gene expression. To demonstrate utility, we employed the SGE system to design, mobilize, and express eight heterologous inorganic carbon uptake pathways in C. necator. We demonstrated all inorganic carbon uptake systems’ upregulated intracellular bicarbonate concentrations under heterotrophic conditions. This work establishes the utility of the SGE strategy for expedited integration and tunable expression of heterologous pathways, and enhances intracellular bicarbonate concentrations in C. necator.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"943–953 943–953"},"PeriodicalIF":3.7,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving Bacillus subtilis as Biological Chassis Performance by the CRISPR Genetic Toolkit 利用 CRISPR 基因工具包提高枯草芽孢杆菌作为生物底盘的性能

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-03-04 DOI: 10.1021/acssynbio.4c0084410.1021/acssynbio.4c00844

Xianhai Cao, Xiaojuan Wang, Ruirui Chen, Lu Chen*, Yang Liu* and Meng Wang*,

{"title":"Improving Bacillus subtilis as Biological Chassis Performance by the CRISPR Genetic Toolkit","authors":"Xianhai Cao, Xiaojuan Wang, Ruirui Chen, Lu Chen*, Yang Liu* and Meng Wang*, ","doi":"10.1021/acssynbio.4c0084410.1021/acssynbio.4c00844","DOIUrl":"https://doi.org/10.1021/acssynbio.4c00844https://doi.org/10.1021/acssynbio.4c00844","url":null,"abstract":"Bacillus subtilis is the model Gram-positive and industrial chassis bacterium; it has blossomed as a robust and promising host for enzyme, biochemical, or bioflocculant production. However, synthetic biology and metabolic engineering technologies of B. subtilis have lagged behind the most widely used industrial chassis Saccharomyces cerevisiae and Escherichia coli. CRISPR (an acronym for clustered regularly interspaced short palindromic repeats) enables efficient, site-specific, and programmable DNA cleavage, which has revolutionized the manner of genome editing. In 2016, CRISPR technology was first introduced into B. subtilis and has been intensely upgraded since then. In this Review, we discuss recently developed key additions to CRISPR toolkit design in B. subtilis with gene editing, transcriptional regulation, and enzyme modulation. Second, advances in the B. subtilis chassis of efficient biochemicals and proteins with CRISPR engineering are discussed. Finally, we conclude with perspectives on the challenges and opportunities of CRISPR-based biotechnology in B. subtilis, wishing that B. subtilis can be comparable to traditional industrial microorganisms such as E. coli and S. cerevisiae someday soon.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":"14 3","pages":"677–688 677–688"},"PeriodicalIF":3.7,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143667049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0