ACS Synthetic Biology最新文献_第9页

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Euphrasie Munier-Lépinay, Coline Amaro-Lauer, Denis Faure, Anthony Quéro, David Mathiron, Mounia Khelifa, Sylvain Laclef* and Serge Pilard*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Caiping Jiang, Kairui Zhai, R. Clay Wright and Juhong Chen*,

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Engineered Lactiplantibacillus plantarum WCFS1 as a Biosensor Probe for Lung Cancer. 工程植物乳杆菌WCFS1作为肺癌生物传感器探针

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-07-03 DOI: 10.1021/acssynbio.5c00186

Michael Brasino, Eli Wagnell, Elise C Manalo, Samuel Drennan, Jared M Fischer, Justin Merritt

{"title":"Engineered Lactiplantibacillus plantarum WCFS1 as a Biosensor Probe for Lung Cancer.","authors":"Michael Brasino, Eli Wagnell, Elise C Manalo, Samuel Drennan, Jared M Fischer, Justin Merritt","doi":"10.1021/acssynbio.5c00186","DOIUrl":"10.1021/acssynbio.5c00186","url":null,"abstract":"Lung cancer is exceedingly difficult and costly to detect early, leading to delayed diagnosis, limited treatment options, and high patient mortality. Tumor-secreted molecules are useful in identifying early disease but are difficult to detect when diluted in accessible bodily fluids. Here, we demonstrate a low-cost, minimally invasive method to probe the lungs for disease using genetically engineered Lactiplantibacillus plantarum bacteria as living biosensors. When delivered to the lungs of mice, the engineered bacteria remained transcriptionally active for several hours and were cleared without colonization. Nanoluciferase secreted by bacteria from within the lungs was subsequently detected in mouse urine. Bacteria were engineered to secrete nanoluciferase in response to a model peptide excreted by a mouse lung cancer cell line, allowing the bacteria to detect tumors formed from these cells in the lungs of mice. Finally, biosensor bacteria were also able to detect a secreted protease overexpressed in adenocarcinoma using a probe protein that is cleaved to release a bacterial pheromone peptide. These results indicate that genetically engineered commensal bacteria yield tremendous promise as living biosensors for early detection screens of lung cancer.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2774-2787"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Tao Wang, Shuwen Ding, Jiao Xu, Guohao Cai, Yiqing Zhang, Yingxuan Qi, Yujia Jiang, Ping Zhang, Tianjing Wang, Fengxue Xin, Tao Shen* and Guannan Liu*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Yuling Song, Yifei Liu, Qingyan Li, Lei Chen*, Zhe Sun* and Xueli Zhang*,

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IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18

Michael Brasino*, Eli Wagnell, Elise C. Manalo, Samuel Drennan, Jared M. Fischer and Justin Merritt,

引用次数: 0

Chemical Control of Protease Activity and Gene Expression Using a Biotin-Released Inhibitory Domain. 利用生物素释放抑制结构域化学控制蛋白酶活性和基因表达。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 DOI: 10.1021/acssynbio.5c00224

Anna M Van Keuren, Casey T Simoes, Chandra L Tucker

引用次数: 0

A Tn5 Transposase-Based System for High-Efficiency Genome-Wide Gene Activation in Escherichia coli. 基于Tn5转座酶的大肠杆菌高效全基因组基因激活系统

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-06-17 DOI: 10.1021/acssynbio.5c00170

Yuling Song, Yifei Liu, Qingyan Li, Lei Chen, Zhe Sun, Xueli Zhang

{"title":"A Tn5 Transposase-Based System for High-Efficiency Genome-Wide Gene Activation in Escherichia coli.","authors":"Yuling Song, Yifei Liu, Qingyan Li, Lei Chen, Zhe Sun, Xueli Zhang","doi":"10.1021/acssynbio.5c00170","DOIUrl":"10.1021/acssynbio.5c00170","url":null,"abstract":"Deciphering gene function to understand the genetic basis of microbial phenotypes in a high-throughput manner is crucial for bacterial engineering. However, efficient tools for generating genome-wide gene activation mutant libraries to enable gain-of-function analyses remain limited. Here, we developed a Tn5 transposase-based system for efficient genome-wide gene activation in Escherichia coli. The cargo DNA incorporated a tetracycline-inducible promoter Ptet and a kanamycin resistance gene, enabling selective growth and conditional gene activation. The system achieved near-random integration with an insertion frequency of approximately 2.83 × 107 cfu/μg DNA. Both in vitro and in vivo transposition results demonstrated the effective activation of specific and nonspecific genes. Using this system, we identified three putative transporters that, despite being unrelated to glycine export, significantly enhanced glycine resistance in E. coli. These results highlight the utility of this method for genotype-phenotype mapping and strain optimization, offering a powerful tool for synthetic biology and functional genomics.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2753-2763"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UV-Resistant Cell-Free Reactions with Synthetic Melanin Additives. 合成黑色素添加剂的抗紫外线无细胞反应。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-06-24 DOI: 10.1021/acssynbio.5c00212

Lauren M Irie, Dylan M Brown, Julius B Lucks, Nathan C Gianneschi

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Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a Host for Lactose Valorization. 奶牛分离物的测序揭示了马氏克卢维菌作为乳糖增殖宿主的作用。

IF 3.7 2区生物学

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-07-08 DOI: 10.1021/acssynbio.5c00096

Mackenzie Thornbury, Adrien Knoops, Iain Summerby-Murray, James Dhaliwal, Sydney Johnson, Joseph Christian Utomo, Jaya Joshi, Lauren Narcross, Gabriel Remondetto, Michel Pouliot, Malcolm Whiteway, Vincent J J Martin

{"title":"Sequencing of a Dairy Isolate Unlocks Kluyveromyces marxianus as a Host for Lactose Valorization.","authors":"Mackenzie Thornbury, Adrien Knoops, Iain Summerby-Murray, James Dhaliwal, Sydney Johnson, Joseph Christian Utomo, Jaya Joshi, Lauren Narcross, Gabriel Remondetto, Michel Pouliot, Malcolm Whiteway, Vincent J J Martin","doi":"10.1021/acssynbio.5c00096","DOIUrl":"10.1021/acssynbio.5c00096","url":null,"abstract":"The use of genetically modified nonconventional yeast provides significant potential for the bioeconomy by diversifying the tools available for the development of sustainable and novel products. In this study, we sequenced and annotated the genome of Kluyveromyces marxianus Y-1190 to establish it as a platform for lactose valorization. The strain was chosen for rapid growth on lactose-rich dairy permeate, high transformation efficiency, and ease of culturing in bioreactors. Genomic sequencing revealed that K. marxianus Y-1190 possesses single nucleotide polymorphisms associated with efficient lactose metabolism. The strain is diploid with notable genomic heterogeneity, which appears to be critical for its robust growth and acid tolerance. To further exploit this platform strain, we developed protocols for gene and chromosome manipulation using CRISPR editing, constructed and validated a series of promoters compatible with MoClo vectors, and designed synthetically inducible promoters for K. marxianus. These tools enable precise control over gene expression, allowing for the tailored optimization of metabolic pathways and production processes. The synthetic promoters provide flexibility for dynamic expression tuning, while the CRISPR-based editing protocols facilitate targeted genetic modifications with high efficiency. Together, these advancements significantly enhance the genetic toolbox for K. marxianus, positioning it as a versatile platform for industrial biotechnology. These tools open new opportunities for the sustainable production of biobased chemicals, fuels, and high-value products, leveraging lactose-rich feedstocks to contribute to a circular economy.","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":"2667-2680"},"PeriodicalIF":3.7,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0