Journal of Medical Microbiology and Diagnosis最新文献

Occurrence of Multidrug Resistance Escherichia coli and Other Bacteria Species Associated with Urinary Tract Infection in Two Geopolitical Zones of Ondo State, Nigeria 尼日利亚翁多州两个地缘政治地区与尿路感染相关的多重耐药大肠杆菌和其他细菌种类的发生

Journal of Medical Microbiology and Diagnosis Pub Date : 2019-05-15 DOI: 10.4172/2161-0703.1000302

Ogunyemi Tm, T. O. Adejumo, F. Olajubu, Titilayo Modupe Waire

{"title":"Occurrence of Multidrug Resistance Escherichia coli and Other Bacteria Species Associated with Urinary Tract Infection in Two Geopolitical Zones of Ondo State, Nigeria","authors":"Ogunyemi Tm, T. O. Adejumo, F. Olajubu, Titilayo Modupe Waire","doi":"10.4172/2161-0703.1000302","DOIUrl":"https://doi.org/10.4172/2161-0703.1000302","url":null,"abstract":"Urinary tract infection (UTI) is a common bacterial infection known to affect different parts of the urinary tract of both male and female. Escherichia coli have been found to be responsible for causing 80% to 90% of the infection. An investigation was carried out to determine the prevalence of bacteria, especially E. coli implicated in UTI, and to ascertain their antibiotics susceptibility pattern. Early morning mid-stream urine samples were collected from 250 patients aged 18 to 60 years, between March and July of 2016 from 5 major Hospitals in the study location. The isolates were identified using standard microbiological methods and susceptibility tests were carried out using ten antibiotics. Results Showed that 65 (30.7%) of the isolates were E. coli. Pseudomonas aeruginosa 45 (21.2%), Klebsiella pneumoniae 42 (19.8%), Staphylococcus aureus 32 (15.1%) and Proteus mirabilis 28 (13.2%). The percentages of resistance of E. coli isolates to antimicrobial agents were chloramphenicol (64.9%), sparfloxacin (59.5%), ciprofloxacin (73.0%), septrin (73.0%), amoxacillin (91.9%), augmentin (83.8%), gentamycin (48.7%), perfloxacin (40.5%), ofloxacin (40.5%) and streptomycin (54.1%). The need for constant antimicrobial susceptibility surveillance by health managements system that will help clinicians to provide safe and effective therapy is advocated.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"39 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132281026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identical Subsequences of Contiguous Amino Acids in Influenza Virus Hemagglutinin and in Human Proteins 流感病毒血凝素和人类蛋白质中连续氨基酸的相同子序列

Journal of Medical Microbiology and Diagnosis Pub Date : 2019-05-10 DOI: 10.4172/2161-0703.1000301

J. Weltman

{"title":"Identical Subsequences of Contiguous Amino Acids in Influenza Virus Hemagglutinin and in Human Proteins","authors":"J. Weltman","doi":"10.4172/2161-0703.1000301","DOIUrl":"https://doi.org/10.4172/2161-0703.1000301","url":null,"abstract":"Background: Influenza virus is a significant public health problem throughout the world. Increased insight into the basic biology of the virus may enable the development of more effective anti-influenza preventives and therapeutics. \u0000Methodology: The occurrence of specific amino acid subsequences in H1N1 and H3N2 influenza virus hemagglutinins was used for joint detection of those subsequences in human proteins. Only subsequences consisting of at least 5 contiguous amino acids were considered for further study. \u0000Results: Ten H1N1 hemagglutinin amino acid subsequences and nine H3N2 hemagglutinin amino subsequences were identified as also occurring in proteins of human origin. The length of the subsequences selected for further study, ranged from 5 contiguous amino acids to 8 contiguous amino acids. \u0000Conclusion: The joint occurrence of amino acid subsequences in influenza hemagglutinins and in human proteins may help explain the relatively low efficacy of current anti-influenza vaccines. It is proposed that the identification of the joint subsequences may be useful for the improved design of anti-influenza therapeutics and especially anti-influenza vaccines.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114263113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparison of Two Enrichment Broth Medium for the Isolation and Identification of Streptococcus agalactiae from Vaginal Swabs 两种浓汤培养基分离鉴定阴道拭子无乳链球菌的比较

Journal of Medical Microbiology and Diagnosis Pub Date : 2019-04-30 DOI: 10.4172/2161-0703.1000300

Dat Tran Huu, D. Le, N. T. Ha, Hoa Nguyen Minh

引用次数: 0

Kodamaea ohmeri, An Emerging Yeast in Tunisia: First Identification in Three Case Reports and Literature Review Kodamaea ohmeri，一种新兴酵母在突尼斯:首次鉴定的三个病例报告和文献综述

Journal of Medical Microbiology and Diagnosis Pub Date : 2019-04-05 DOI: 10.4172/2161-0703.1000299

L. Mtibaa, H. Souid, B. Jemli, Z. Hajjej, Chiraz Halweni, A. Rebai, R. B. Mhamed, K. Akkari, M. Ferjani

引用次数: 2

Effectivity of Caspofungin on the Resistant Isolates of Candida albicans 卡泊真菌素对白色念珠菌耐药株的疗效

Journal of Medical Microbiology and Diagnosis Pub Date : 2019-02-15 DOI: 10.4172/2161-0703.1000293

Nusrat Perween, H. Khan, Nazish Fatima

引用次数: 1

Current and Novel Methods in Clinical Microbiology: Advantages and Pitfalls when Facing the Menace of Antimicrobial Resistance 临床微生物学的新方法与新方法:面对抗菌素耐药性威胁的优势与缺陷

Journal of Medical Microbiology and Diagnosis Pub Date : 2018-03-09 DOI: 10.4172/2161-0703.1000275

C. Pina-Vaz, Azevedo Mm, A. Rodrigues

{"title":"Current and Novel Methods in Clinical Microbiology: Advantages and Pitfalls when Facing the Menace of Antimicrobial Resistance","authors":"C. Pina-Vaz, Azevedo Mm, A. Rodrigues","doi":"10.4172/2161-0703.1000275","DOIUrl":"https://doi.org/10.4172/2161-0703.1000275","url":null,"abstract":"Microbiological clinical methods were the object of this review. According to WHO antimicrobial resistance is a real and global threat. Researchers aim to develop rapid methods for identification and antimicrobial susceptibility (AST). Actually, most automated solutions available in clinical laboratories are based upon the study of the microbial ability to grow so, take a long time to give results despite its advantages. Molecular tests only detect target genes but are especially useful regarding identification of difficult or slow growing microorganisms. MALDI-TOF started a real revolution in microbial identification since it is growth independent and highly sensitive and specific. Regarding susceptibility evaluation, due to its inherent complexity, molecular or proteomic tests provides answers to known and molecular characterized mechanisms, requiring its prior knowledge. Flow cytometry is an excellent tool that, coupled with specific fluorescent antibodies can be used to identify microorganisms. Moreover, it can help to unveil susceptibility profile. AST phenotype is provided following incubation of the cells for short period (60 minutes) with antimicrobial drugs and fluorescent probes, with excellent correlation with classic AST methods. Furthermore, it can elucidate about the most relevant mechanism of resistance in a functional assay. Novel methods are under study namely sophisticated methods for growth detection like, weighing bacteria by vibrating cantilevers, isothermal microcalorimetry method, simple spectroscopic biomarkers and plasmonic imaging and tracking are discussed. We are close to a change of the paradigm in the clinical laboratory work flow microbiology considering especially MALDI-TOF for identification and flow cytometry for AST/assessment of mechanisms of resistance.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127571456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of ELISA, Antigenemia Assay and Nested PCR Monitoring Techniques for Detection of Cytomegalovirus Infection in Renal Transplantation Patients ELISA、抗原血症法和巢式PCR检测肾移植患者巨细胞病毒感染的比较

Journal of Medical Microbiology and Diagnosis Pub Date : 2018-02-23 DOI: 10.4172/2161-0703.1000274

E. Moghanloo, V. Babaei, S. Rezaei, Leila Khanmirzaei, Tina Delsouz Bahri, H. Z. Gohardani, S. Teimourian

{"title":"Comparison of ELISA, Antigenemia Assay and Nested PCR Monitoring Techniques for Detection of Cytomegalovirus Infection in Renal Transplantation Patients","authors":"E. Moghanloo, V. Babaei, S. Rezaei, Leila Khanmirzaei, Tina Delsouz Bahri, H. Z. Gohardani, S. Teimourian","doi":"10.4172/2161-0703.1000274","DOIUrl":"https://doi.org/10.4172/2161-0703.1000274","url":null,"abstract":"Human cytomegalovirus (HCMV) can be transmitted through blood transfusion and organ transplantation and could be cause of some complication in solid-organ transplant recipients. Current study is aimed to compare the sensitivity and Specificity of ELISA, Antigenemia assay and nested PCR methods to detection of Cytomegalovirus infection in renal transplantation patients. \u0000In this study blood samples were collected from 200 renal transplant recipients’ patients. \u0000DNA was extracted by commercial kit and Nested PCR was done by 2 pairs of internal and external primers. Anti CMV antibodies (IgM and IgG) were detected by ELISA and CMV-pp65 antigenemia assay (Ag) was used to detect CMV antigens. The sensitivity and Specificity of each test and all the methods together were evaluated, and SPSS software was used to analysis of data. \u0000From 200 patients, 193 (96.5%) were positive for CMV antibodies with the Specificity of 100 and sensitivity of 97.76%. 120 (60%) and 25 (12.5) samples were positive by nested PCR and Ag assay with the Specificity of 94.49 and 78.12 and sensitivity of 94.49 and 78.12, respectively. \u0000In the case of early diagnosis of the disease, nested PCR diagnose the infection 14 years earlier than Ag assay and was consistently positive, whereas false negative results were frequently observed with the pp65 Ag assay. The sensitivity and specificity of the two methods combined detection for CMV infection were 96.76% and 99.89%. ELISA can be used as a screening reliable detection test for CMV infection in recipient especially when PCR is unavailable. \u0000Combination of ELISA and CMV-PCR methods, provide a more effective method to monitor CMV infection.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131309432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of Biomed InTray ® Colorex MRSA with BD ESwab Collection Kit/ BBL™ CHROMagar ® MRSA II biomeed InTray®Colorex MRSA与BD ESwab Collection Kit/ BBL™CHROMagar®MRSA II的比较评价

Journal of Medical Microbiology and Diagnosis Pub Date : 2018-02-19 DOI: 10.4172/2161-0703.1000273

Kiran Bijlani, M. Gómez, Rowena D. Matias, A. Najafi, Ron Najafi, S. Arumugam

引用次数: 1

Tissue Changes in Experimental Cryptococcosis in Immunocompetent and Immunodeficient Murine Model 免疫功能正常和免疫缺陷小鼠模型隐球菌病的组织变化

Journal of Medical Microbiology and Diagnosis Pub Date : 2018-01-29 DOI: 10.4172/2161-0703.1000271

E. G. Silva, J. N. Cavalcanti, F. C. Viani, S. M. D. S. Silva, A. L. T. Dias

{"title":"Tissue Changes in Experimental Cryptococcosis in Immunocompetent and Immunodeficient Murine Model","authors":"E. G. Silva, J. N. Cavalcanti, F. C. Viani, S. M. D. S. Silva, A. L. T. Dias","doi":"10.4172/2161-0703.1000271","DOIUrl":"https://doi.org/10.4172/2161-0703.1000271","url":null,"abstract":"Cryptococcosis is a subacute or chronic disease caused through the inhalation of infectious particles from the opportunistic yeast Cryptococcus neoformans spp. The objective of the present study was to evaluate cryptococcosis in a murine model Immunocompetent (BALB/c), as well as in a model with combined immunodeficiency (SCID) through histopathological analyzes of pulmonary and cerebral tissues. After intravenous inoculation with 3.0 × 105 viable yeast cells the animals were euthanized daily for evaluation. The study period was 15 days. There were no significant changes in lung tissue in immunocompetent murine model (BALB/c). While in brain tissue, it was observed: congested vessel, evolving when C. neoformans was visualized in the meningeal area, and a large area of ischemia, which evolved throughout the studied period culminating on the 15th day of inoculation with visualization of the yeast in the meningeal and parenchyma. In SCID model, twenty-four hours after inoculation were observed in the lung tissue, hemorrhagic areas and a discrete neutrophilic inflammatory infiltrate, presence of discrete congestion in the lung, diffuse hemorrhage, edema and intense quantity of yeast were observed on the wall of the capillary at 11 days after inoculation. In brain tissue discrete area necrosis liquefaction was observed, focal well as the presence of C. neoformans, interspersed with fragments of necrotic cells was observed. On day 11 after inoculation were large areas of liquefaction necrosis associated with the formation of cavities in the parenchyma and an intense quantify of the yeast. Histopathological examination is one of the techniques usually used in the definitive diagnosis of cryptococcosis.","PeriodicalId":269971,"journal":{"name":"Journal of Medical Microbiology and Diagnosis","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115552328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leukocyte Esterase as Predictor of Urine Culture Result 白细胞酯酶作为尿培养结果的预测因子

Journal of Medical Microbiology and Diagnosis Pub Date : 2017-11-17 DOI: 10.4172/2161-0703.1000264

W. Rieker, E. Reilly, Balamurugan Pandiyan, S. Lom, A. Merry

引用次数: 0