LIFE: International Journal of Health and Life-Sciences最新文献

{"title":"ANTIOXIDANT ACTIVITY OF CATECHINS ISOLATE OF UNCARIA GAMBIER ROXB IN MALE RATS","authors":"M. Y. Musdja, H. Rahman, D. Hasan","doi":"10.20319/LIJHLS.2018.42.3446","DOIUrl":"https://doi.org/10.20319/LIJHLS.2018.42.3446","url":null,"abstract":"The aims of this study were to determine the antioxidant activity of catechins isolates of gambir by measuring the levels of Malondialdehid (MDA) in male white rats. Methods: Catechins of gambir were isolated by using partition method with ethyl acetate solvent. Gambir quality was determined based on National Standard of Indonesia: SNI 01-3391-2000. The yield of catechins obtained from the gambir isolate was determined as (+)-catechins  by comparing with standard  (+)-catechins and measured by a spectrophotometer UV-VIS at wavelength 279 nm. A total of 25 male rats were divided into 5 groups. Administration of catechins was suspended in  0.5% Na CMC with dose of 5 mg/kg b.w, 10 mg/kg b.w and 20 mg/kg b.w, as  positive control was used the suspension of vitamin E, 20 mg/kg b.w in Na-CMC 0.5% and as  negative control was used 0.5% Na-CMC suspension. Administration of test preparations was performed per oral, 1 times per day for 7 days. On the eighth day, the rats were made become oxidative stress with swimming in water about 1 hour, until almost drowned.  MDA levels of rats in each group were measured by comparing MDA levels, before were given test preparation on first day and after were given test preparations on the eighth day. The MDA level of serum rats  were measured based on Wills method. Result: The statistical test of catechins isolate of gambir showed that all dosage of catechins isolate test of gambir had antioxidant effect and had significant difference to negative control (p <0.05). Doses 5 and 10 mg/kg b.w did not show significant differences with  vitamin A as positive control, while Dose 20 mg/kg b.w  gave the strongest antioxidant effect and had significant difference with positive control (p <0.05). Conclusion: Catechins of Gambir has stronger potential as an antioxidant than vitamin A. Article DOI:  https://dx.doi.org/10.20319/lijhls.2018.42.3446 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"159 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114107606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASSESSMENT OF LOADING OF ASCORBYL-2-PHOSPHATE AND 6-O-PALMITOYLASCORBIC ACID IN CETYL DIMETHICONE COPOLYOL BASED W/O/W EMULSIONS","authors":"Hira Khan, Atif Ali","doi":"10.20319/LIJHLS.2018.42.0114","DOIUrl":"https://doi.org/10.20319/LIJHLS.2018.42.0114","url":null,"abstract":"W/O/W emulsions with multifaceted nature ensure the ability to incorporate lipophilic as well as hydrophilic compounds based on their solubility. Loading of these compounds in different phases of W/O/W emulsion may affect their constancy. This study was aimed to evaluate the constancy of Ascorbyl-2-phosphate and 6-O-Palmitoylascorbic acid in W/O/W emulsions (F 1 and F 2 ). F 1 and F 2 samples containing Ascorbyl-2-phosphate and 6-O-Palmitoylascorbic acid  in different phases of cetyl dimethicone copolyol based W/OW emulsions were analyzed for globule size, rheological behavior and HPLC, immediately after preparation and then at different storage conditions (8°C, 25°C, 40°C and 40°C+75%RH) for 90 days. Rheological analysis showed that at accelerated storage conditions F 1 and F 2 had shear thinning behaviour of varying shear stress with no influence of location of functional ingredients in the carrier system. Microscopic analysis showed increase in globule size with time, especially at higher temperatures while decreased at low temperatures. HPLC analysis at the end of 90 days showed that 6-O-Palmitoylascorbic acid and Ascorbyl-2-phosphate were almost stable in F 1 and F 2 W/OW emulsions with no influence of their location in a carrier system. F 1 and F 2 W/OW emulsions were found stable carriers for Ascorbyl-2-phosphate and 6-O-Palmitoylascorbic acid. This study may contribute to the improvement of formulations with Ascorbyl-2-phosphate and 6-O-Palmitoylascorbic acid to enhance their cosmetic benefits and also provides the direction to evaluate the stability with different concentrations of these compounds. Article DOI:  https://dx.doi.org/10.20319/lijhls.2018.42.0114 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124296144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE INFLUENCE OF PLASMIDS AND OTHER MGE’S OVER ANTIBIOTIC RESISTANCE IN MULTIDRUG RESISTANT STAPHYLOCOCCUS AUREUS ISOLATED FROM HIV+ PATIENTS","authors":"N. NihalAhmedK, B. Daniel, Jancy Varghese","doi":"10.20319/LIJHLS.2017.33.6987","DOIUrl":"https://doi.org/10.20319/LIJHLS.2017.33.6987","url":null,"abstract":"Bacterial opportunistic infections are common among immunocompromised patients. Antibiotic resistance hinders efforts to treat such infections. Our objectives are to detect the prevalence of Staphylococcus aureus in immunocompromised HIV patients, to analyze their antibiogram, to correlate antibiotic resistance with the presence of plasmids and to identify crucial antibiotic resistance genes and understand their connectivity with MGEs. Bacterial strains were collected from HIV+ve patients. Pure cultures of Staphylococcus aureus were isolated and its clones were selected for further studies. Their Antibiogram and plasmid profiles were analysed. Vancomycin resistant strains were selected and PCR was conducted to trace the presence of Van A in both the gDNA and plasmids. There wasn’t much correlation between antibiotic resistance and plasmid profile. The most resistant strains didn’t have high copy number plasmids than the less resistant ones. Thus Plasmids weren’t the sole determinants of antibiotic resistance. This led us to explore other MGEs that could aid in conferring antibiotic resistance -an MGE that could be stationed on both gDNA & Plasmids. Literature shows that b-lactam resistance is due to transposons. A  Tn3  family that’s non-constitutive, replicative DNA transposon. This transposon stays on the gDNA further lowering the possibilities of losing it via vertical gene transfer. VanA is a part of similar transposon Tn 1546 of the Tn3 family.  PCR for Van A on Vancomycin resistant strains showed positive results both on gDNA and plasmids. The outcomes open up other possible pathways to tackle MDR strains than antibiotics. Article DOI: https://dx.doi.org/10.20319/lijhls.2017.33.6987 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133908211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASSESSMENT OF LOCAL HEALTH SYSTEM’S RESPONSIVENESS TO TUBERCULOSIS IN SELECT MUNICIPALITIES IN PHILIPPINES","authors":"R. Nieva","doi":"10.20319/lijhls.2017.32.178193","DOIUrl":"https://doi.org/10.20319/lijhls.2017.32.178193","url":null,"abstract":"Tuberculosis (TB) remains one of the leading causes of morbidity and mortality in Philippines. The structure of the TB control delivery system in the Philippines is devolved that has led to some significant gaps in responding to the health needs of the population across the country.The study was carried out to assess how municipalities implement TB programs using the local health systems model; and to document existing policies and initiatives in the local government units. It also applied a desk review of existing practices in 20 municipalities, plus key informant interviews with 15 mayors, 20 municipal health officers and 15 TB coordinators. Findings indicated that less than a quarter of municipalities had investment plan for health on TB and 41% had local policy supporting TB program. Additionally, 25% had specifically allocated budget for TB, 82% had their facilities accredited by PhilHealth, and 59% had inadequate number of health workers for TB program. About 18% had x-ray facilities, and 41% had enough supply and buffer of medicines. Eighteen per cent (18%) had actively engaged private and government hospitals for TB program, and 100% did not screen TB patients for HIV. Data showed the gaps in the municipal health systems to address TB issues. Thus, local leaders must be capacitated and supported by stakeholders to strengthen service delivery for TB. This underscores the big role of local leadership in TB prevention and control program being the frontline policy maker and service provider.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133297867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MEASURING HEAD NURSES’ COACHING PRACTICE: MODIFYING AND TESTING THE INSTRUMENT FOR ASSESSING MANAGERIAL COACHING SKILLS","authors":"Romiko, Apinya Jumpamool","doi":"10.20319/LIJHLS.2016.S21.1835","DOIUrl":"https://doi.org/10.20319/LIJHLS.2016.S21.1835","url":null,"abstract":"Managerial coaching has been reported as a successful leadership development approach that has potential benefit for nurses. It also was recognized as a powerful tool to promote personal and professional development in the nursing profession. Therefore, there is an increasing needs for reliable and valid assessment tools to measure the coaching skills of nurses in managerial positions. This study reviewed and revised an existing instrument created by (Park, McLean, and Yang, 2007) which measured coaching skills of managers in business and industrial organization. In order to adapt the original instrument to the nursing profession, this study firstly identified the five primary dimensions of the managerial coaching skills of head nurses through both qualitative and quantitative approaches. Review experts and a pilot study were then performed to ensure the reliability and validity, and validated the revised instrument of managerial coaching skills. The results of this study indicate the revised managerial caching skill instrument demonstrates good reliability and validity for measuring the managerial coaching skill of head nurses in a hospital setting. Hospitals can administer and use the revised instrument as an assessment tool to evaluate their nurse managers’ coaching skills, and identify the areas for improvement. This allow the head nurses to more effectively manage and support the staffs’ development. Article DOI: http://dx.doi.org/10.20319/lijhls.2016.s21.1836 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130367424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE PREVALENCE OF HYPERTENSION, OBESITY, DIABETES AND JOB SATISFACTION AMONG PUBLIC HEALTH WORKERS IN CENTRAL PROVINCE, SRI LANKA","authors":"C. Senevirathne, P. Katulanda, N. Dhanapala","doi":"10.20319/LIJHLS.2015.12.2547","DOIUrl":"https://doi.org/10.20319/LIJHLS.2015.12.2547","url":null,"abstract":"Aim of this study was to assess the prevalence of hypertension, obesity, overweight, diabetes and job satisfaction among public health workers in the Central province, Sri Lanka. This cross sectional study was conducted in three districts in the Central Province, Sri Lanka. Data were collected from public health workers (PHW) (n= 336). BMI, blood pressure and random blood sugar were measured. General information and self-reported job satisfaction and coping strategies were collected by a self-administered questionnaire. Mean age of PHW was 42.5 (SD 10.3, CI=95%). There were 302 females (89%) in the group. Prevalence of overweight and obesity among PHW were 29. 5% and 16.4% respectively. Among the group 26.4 % individuals (n=89) were hypertensive and 21 (6%) individuals were living with diabetes. 57.1% of individuals (n=192) reported with moderate pressure on their work. Extremely satisfied, very satisfied and moderately satisfied unsatisfied and not at all satisfied were 31.5%, 61%, 5.4 %, 1.2% and 0.6 respectively. Field work, work load, working environment, income were found to be as the common sources for stressful circumstances at work. Study revealed that overweight and obesity prevalence among public health workers in the Central Province is considerable. Significant amount of public health workers were hypertensive and living with diabetes. More than half from the group had a moderate satisfaction with their job. Article DOI:  https://dx.doi.org/10.20319/lijhls.2015.12.2547 This work is licensed under the Creative Commons Attribution-Non-commercial 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.","PeriodicalId":260489,"journal":{"name":"LIFE: International Journal of Health and Life-Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129270466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}