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A method for the preparation of undigested bacterial cell walls 一种制备未消化细菌细胞壁的方法
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1961-07-01 DOI: 10.1002/JBMTE.390030205
A. Yoshida, C. Hedén, B. Cedergren, L. Edebo
{"title":"A method for the preparation of undigested bacterial cell walls","authors":"A. Yoshida, C. Hedén, B. Cedergren, L. Edebo","doi":"10.1002/JBMTE.390030205","DOIUrl":"https://doi.org/10.1002/JBMTE.390030205","url":null,"abstract":"The combination of low-temperature disintegration with gradient and pile centrifugation makes possible the preparation of bacterial cell walls under conditions preventing digestion.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"180 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1961-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125958855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Cation exchangers on a sugar-beet pulp base. Application for decontaminating radioactive waste water 甜菜浆碱上的阳离子交换剂。放射性废水净化应用
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1961-04-01 DOI: 10.1002/JBMTE.390030103
W. T. J. P. Langenhorst, M. Tels, J. C. Vlugter, H. I. Waterman
{"title":"Cation exchangers on a sugar-beet pulp base. Application for decontaminating radioactive waste water","authors":"W. T. J. P. Langenhorst, M. Tels, J. C. Vlugter, H. I. Waterman","doi":"10.1002/JBMTE.390030103","DOIUrl":"https://doi.org/10.1002/JBMTE.390030103","url":null,"abstract":"Experiments were carried out on the use of sugar beet pulp, which is a weakly acidic cation-exchange material with a capacity of about 0.62 mg equivalent per g of dry matter. It was found that sugar beet pulp adsorbs the salts of alkaline earth metals selectively tn the presence of both sodium and lathanum, and it successfully decontaminated a solution containing barium-140 and lanthanum-140 with an activity of about 10/sup -2/ mu C per ml. Treatment of the pulp with formaldehyde and hydrochloric acid greatly reduced the amount of water bound to the pulp and increased the exchange capacity six-fold. Treatment with formaldehyde, hydrochloric acid, and dilute sulfuric acid also produced an exchange material with good mechanical and filtration properties and with a capacity of about 1.3 mg equtvalent per g of dry matter. (Public Health Eng. abstr., 42: No. 3, March 1962)","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1961-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127640843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Ion‐exchange chromatography of the streptomycin complex 链霉素络合物的离子交换色谱
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1961-04-01 DOI: 10.1002/JBMTE.390030109
J. N. Pereira
{"title":"Ion‐exchange chromatography of the streptomycin complex","authors":"J. N. Pereira","doi":"10.1002/JBMTE.390030109","DOIUrl":"https://doi.org/10.1002/JBMTE.390030109","url":null,"abstract":"Various studies have revealed the presence of several maltolproducing compounds in streptomycin preparations. These previous studies have utilized the techniques of counter-current distribution, column chromatography, paper chromatography, and electrophoresis. A system of cation-exchange chromatography has been reported which separates, at least partially, six components of the streptomycin complex. This technique involves the use of a gradient elution procedure. \u0000 \u0000In addition to five maltol-producing compounds, another member of the complex is reported. The possible significance of such a compound is discussed.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1961-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120770859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Clinical‐size dextran by direct fermentation (with NRRL B–1351 Streptococcus sp.) 临床尺寸葡聚糖直接发酵(用NRRL B-1351链球菌sp.)
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-12-01 DOI: 10.1002/JBMTE.390020402
S. Rogovin, F. R. Senti, R. G. Benedict, H. Tsuchiya, P. Watson, R. Tobin, V. Sohns, M. Slodki
{"title":"Clinical‐size dextran by direct fermentation (with NRRL B–1351 Streptococcus sp.)","authors":"S. Rogovin, F. R. Senti, R. G. Benedict, H. Tsuchiya, P. Watson, R. Tobin, V. Sohns, M. Slodki","doi":"10.1002/JBMTE.390020402","DOIUrl":"https://doi.org/10.1002/JBMTE.390020402","url":null,"abstract":"","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"244 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120158963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
A new press for the disruption of micro‐organisms and other cells 对微生物和其他细胞的破坏的新压力
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-12-01 DOI: 10.1002/JBMTE.390020409
Lars Edebo
{"title":"A new press for the disruption of micro‐organisms and other cells","authors":"Lars Edebo","doi":"10.1002/JBMTE.390020409","DOIUrl":"https://doi.org/10.1002/JBMTE.390020409","url":null,"abstract":"A new press (the X-press) has been developed for the disintegration of cells in the frozen state (mostly at −25°C). By forcing the frozen cells contained in a cylinder through a hole much smaller than the diameter of the cylinder but much larger than the size of the cells, a satisfactory disintegration can be obtained. \u0000 \u0000By forcing the cells through the hole repeatedly rather than once, a higher degree of disintegration can be achieved. By making the receiver identical to the charge chamber, repeated operations are possible without uncoupling the press. \u0000 \u0000The disintegrated material is very easily recovered—it is simply taken out of the press in the form of a cylinder containing the frozen, disintegrated material which is then easily homogenized. \u0000 \u0000This principle has been found satisfactory for the disintegration of a large number of cells, viz. bacteria, yeasts, moulds, plant and animal cells and, with regard to biochemical questions, it has been found to be superior to existing methods which do not treat the bacteria in the frozen state. The mechanism of the disintegrating action is discussed.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124715199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 158
Studies on the fermentative production of L‐isoleucine L -异亮氨酸发酵生产的研究
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-09-01 DOI: 10.1002/JBMTE.390020310
I. Chibata, M. Kisumi, Y. Ashikaga
{"title":"Studies on the fermentative production of L‐isoleucine","authors":"I. Chibata, M. Kisumi, Y. Ashikaga","doi":"10.1002/JBMTE.390020310","DOIUrl":"https://doi.org/10.1002/JBMTE.390020310","url":null,"abstract":"Several strains of Pseudomonas were selected by the screening test for organisms which are capable of producing L-isoleucine. \u0000 \u0000With these selected bacteria, various cultural conditions suitable for the production and accumulation of L-isoleucine were investigated. As a result, 12–14 mg/ml of L-isoleucine was formed by shake culture in a medium containing α-aminobutyric acid, glucose, cornsteep liquor, urea and inorganic salts.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128505026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Process report: Large‐scale production of foot‐and‐mouth disease virus 过程报告:大规模生产口蹄疫病毒
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-09-01 DOI: 10.1002/JBMTE.390020308
B. Ubertini, L. Nardelli, G. Santero, G. Panina
{"title":"Process report: Large‐scale production of foot‐and‐mouth disease virus","authors":"B. Ubertini, L. Nardelli, G. Santero, G. Panina","doi":"10.1002/JBMTE.390020308","DOIUrl":"https://doi.org/10.1002/JBMTE.390020308","url":null,"abstract":"A pilot plant is described for the mass cultivation of foot-and-mouth disease virus, in vitro, on calf kidney cell monolayers. The pilot plant produces up to 400 litres of virus per week; it can be enlarged with ease to a sufficient capacity to meet any requirements.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124990622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
Submerged growth of cells of higher plants 高等植物细胞的浸没生长
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-09-01 DOI: 10.1002/JBMTE.390020305
L. G. Nickell, W. Tulecke
{"title":"Submerged growth of cells of higher plants","authors":"L. G. Nickell, W. Tulecke","doi":"10.1002/JBMTE.390020305","DOIUrl":"https://doi.org/10.1002/JBMTE.390020305","url":null,"abstract":"The submerged propagation of plant tissue and cell cultures has now reached the stage where mass amounts of material can be produced. In discussing these advances, various aspects of the technology involved are discussed: the methods of isolation and establishment of cultures, the media used for culture and their effects on growth, the systems used for growth and a comparison of their effectiveness. \u0000 \u0000Biochemical differences are shown between cultures grown under in vitro conditions and the organ from which they were derived. These differences are found among amino acids, organic acids, nucleic acids, and sugars. The potential use of the tissue culture approach for the study of the biosynthesis of ‘secondary compounds’ such as alkaloids and steroids is discussed.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"312 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124429674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Continuous culture experiments with saccharomyces rouxii 罗氏酵母菌连续培养实验
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-06-01 DOI: 10.1002/JBMTE.390020211
P. Dawson
{"title":"Continuous culture experiments with saccharomyces rouxii","authors":"P. Dawson","doi":"10.1002/JBMTE.390020211","DOIUrl":"https://doi.org/10.1002/JBMTE.390020211","url":null,"abstract":"Growth of Saccharomyces rouxii, an osmophilic yeast that produces glycerol and arabitol, was investigated using chemically defined media in continuous culture. Total polyol production increased with increasing aeration and growth rate. At very low aeration levels ethanol was produced but as aeration levels were increased ethanol could not be detected, formation of d-arabitol remained constant and formation of glycerol increased. \u0000 \u0000In fully aerobic cultures with a replacement time of twelve hours, nitrogen-limited growth resulted in accumulation of glycerol in the medium. In glucose-limited growth glycerol tended to decrease. It was probably metabolized as a secondary carbon source. The optimum temperature for polyol production was 30°C while that for cell growth was 27°C.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124017616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Electronic computer solution for the MPN equation used in the determination of bacterial populations 用于测定细菌种群的MPN方程的电子计算机解决方案
Journal of Biochemical and Microbiological Technology and Engineering Pub Date : 1960-06-01 DOI: 10.1002/JBMTE.390020205
R. L. Norman, L. Kempe
{"title":"Electronic computer solution for the MPN equation used in the determination of bacterial populations","authors":"R. L. Norman, L. Kempe","doi":"10.1002/JBMTE.390020205","DOIUrl":"https://doi.org/10.1002/JBMTE.390020205","url":null,"abstract":"The utility and the practical precision of the Most Probable Number method for determining bacterial populations is currently restricted by a lack of adequate tables of solved examples or alternatively by an easy and quick solution to individual problems. Where an electronic computer is available, these restrictions need not apply. A computer programme and example solution for tabulated data and individual problems are presented in this paper.","PeriodicalId":259241,"journal":{"name":"Journal of Biochemical and Microbiological Technology and Engineering","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1960-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125763549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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