Ion‐exchange chromatography of the streptomycin complex

Various studies have revealed the presence of several maltolproducing compounds in streptomycin preparations. These previous studies have utilized the techniques of counter-current distribution, column chromatography, paper chromatography, and electrophoresis. A system of cation-exchange chromatography has been reported which separates, at least partially, six components of the streptomycin complex. This technique involves the use of a gradient elution procedure. In addition to five maltol-producing compounds, another member of the complex is reported. The possible significance of such a compound is discussed.