Studies on the fermentative production of L‐isoleucine

Abstract

Several strains of Pseudomonas were selected by the screening test for organisms which are capable of producing L-isoleucine. With these selected bacteria, various cultural conditions suitable for the production and accumulation of L-isoleucine were investigated. As a result, 12–14 mg/ml of L-isoleucine was formed by shake culture in a medium containing α-aminobutyric acid, glucose, cornsteep liquor, urea and inorganic salts.