EPH - International Journal of Biological & Pharmaceutical Science最新文献

{"title":"STUDY OF VIRUSES MATERIAL RNA OR DNA","authors":"H. Kheyrodin","doi":"10.53555/eijbps.v6i1.32","DOIUrl":"https://doi.org/10.53555/eijbps.v6i1.32","url":null,"abstract":"A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Most viruses have either RNA or DNA as their genetic material. The nucleic acid may be single- or double-stranded. The entire infectious virus particle, called a virion, consists of the nucleic acid and an outer shell of protein. The simplest viruses contain only enough RNA or DNA to encode four proteins. A virus is a small parasite that cannot reproduce by itself. Once it infects a susceptible cell, however, a virus can direct the cell machinery to produce more viruses. Most viruses have either RNA or DNA as their genetic material. The nucleic acid may be single- or double-stranded. A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Too we conducted that the genome replication of most DNA viruses takes place in the cell's nucleus. If the cell has the appropriate receptor on its surface, these viruses enter the cell either by direct fusion with the cell membrane ","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125177353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIODIVERSITY OF WILD MEDICINAL PLANTS IN SOME AREAS OF THE SOUTHERN SLOPE OF THE HISSAR RIDGE","authors":"D. Sattarov, S. Vyshegurov, R. Galeev","doi":"10.53555/eijbps.v6i1.31","DOIUrl":"https://doi.org/10.53555/eijbps.v6i1.31","url":null,"abstract":"Are presented the results of studies to identify the biodiversity of wild medicinal plants on the territory of some parts of the southern slope of the Hissar ridge. As a result of researches it was established that in the territory of 11 gorges surveyed (Gusgarf, Ojuk, Gulobod, Gazhne, Semiganch, Safedchashma, Obi-Zugora, Magov, yos, Yavroz and Shamal) 174 species of medicinal plants, which belong to various Botanical taxa, are mainly distributed. This number of plants is formed by representatives of 106 genera, which belong to 46 families, among which representatives of the family Asteraceae (36 species), Rosaceae (19 species), Lamiaceae (16 species). The number of naked species is 11.6 % of the number of described medicinal plants on the territory of the Republic of Tajikistan. The four found species (Rubus caesius L., R. turkestanicus Pavl., Rheum hissaricum Losinsk., R. maximowiczii Losinsk) were plants that are widely eaten by the people.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133893373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STATUS REPORT OF MYCOBACTERIUM TUBERCULOSIS IN REPUBLIC OF MALAWI, CENTRAL AFRICA (14.32’39.954’S AND 35.11’26.269’E)","authors":"V. Thiruvengadam, Carolin Sheril, Loyola Aumose, Mike Mwale, P. E. Mwale, Charles Chazemba, Marvecous Msuzi, Dumilani Njolomole, A. Beza, MemoryNkhata, Neema Thindwa, Sybil Kaduya, Faith, J. Nyirenda, Josophine Chinyama, E. Gama, ChrisyMsisya, Dhummie Kaunda, H. Moyo, Dennis Mdala, Connis Chisale","doi":"10.53555/eijbps.v5i1.30","DOIUrl":"https://doi.org/10.53555/eijbps.v5i1.30","url":null,"abstract":"Worldwide, Tuberculosis has one of the top ten causes of death and the leading cause from a distinct infectious agent (HIV/AIDS). In 2017, MTB has been affected and it was estimated 1.2 1.4 million (WHO, 2018). Globally MTB was estimated 10.0 million people have developed of ailments in 2017, for men 5.8 m and women 1.0 m children. In global they are different age group and overall 90% were adults (aged ≥ 15 years), 9% was affected and living with HIV (72% in Africa including Malawi) and two thirds were in eight countries: India (27%), China (9%), Indonesia (8%), the Philippines (6%), Pakistan (5%), Nigeria (4%), Bangladesh (4%) and South Africa (3%) (WHO, 2018).","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115261945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HYDROGEN PEROXIDE IMPEDES NK CELL AND T CELL MEDIATED CYTOTOXICITY AGAINST COLON CANCER CELL","authors":"S. S. Gounder, Baskar Subramani, Chithra Ramanathan Pullai, Ezzati Radzuan, Arulvasu Chinnasamy","doi":"10.53555/eijbps.v5i1.29","DOIUrl":"https://doi.org/10.53555/eijbps.v5i1.29","url":null,"abstract":"Although hydrogen peroxide (H2O2) is an admirable treatment for many cancer types, but the clinical impact of H2O2 on immune cells is not known. In the present study, we have demonstrated that H2O2 is a crucial factor to determine the cell mediated cytotoxicity of immune cells to the cancer cells. Natural killer (NK) cells and T cells were isolated from healthy donors and were activated and enriched the population at ex-vivo condition. At day14, activated NK and T cells were exposed to colon cancer cell at the ratio of 10:1 (Effector: Target) with or without H2O2 (50 µM and 100µM) treatment for 6 h and 12 h. Cytotoxicity assay showed that cytotolytic ability of NK and T cells was strongly suppressed in the presence of H2O2. H2O2 induced NK and T cell dysfunction was analyzed and results demonstrated that NK and T cells were lose their viability on dose and time dependent manner. Also, flow cytometry analysis revealed that H2O2 significantly suppresses the activation and proliferation CD8 T cell population. Whereas, NK cell subset (CD56bright and CD56dim) was dramatically altered by H2O2. When exposed to H2O2, CD56dim population was considerably increased at different time points.  Hence, we conclude that H2O2 directly or functionally defect the NK and T cell mediated cytotoxicity and however, further studies are warranted to confirm the concept with mild dose of H2O2 with different cancer cell lines.     ","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134127028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPACT OF THE OPERATION CIGLITAZONE ON PPAR ACTIVATION AND APOPTOSIS PROCESS IN MODELS OF GASTROINTESTINAL CANCER IN VITRO","authors":"Aldona Olechowska-Jarząb, A. Targosz, A. Ptak-Belowska","doi":"10.53555/eijbps.v4i1.28","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.28","url":null,"abstract":"Background: Peroxisome proliferator-activated receptors (PPAR-γ) are nuclear transcription factors which affect the stimulation of glucose and lipid metabolism, modulation of inflammation, tissue sensitivity to insulin, immune response, cell proliferation and differentiation. Current research on PPAR-γ receptors is contradictory.  \u0000A significant part of the research suggests that these receptors may be targeted for anti-cancer therapy and have anti-inflammatory properties. Other analyzes speculate on the role of PPAR-γ in promoting cancer. It is therefore important that further studies help to better understand the role of PPAR-γ receptors, which may be relevant in the context of public health and cancer therapy \u0000Methods: The purpose of the study was to determine the effect of ciglitazone (10 μM) on expression of PPAR-γ receptors. In addition, it was investigated whether action on PPAR-γ nuclear receptors with a specific ligand concentration (10 μM of ciglitazone) could lead to increased expression of apoptotic protein (Bcl-2, PKB/Akt) in gastric cancer cells (PANC-1, HT-29) in models In vitro. The effects of ciglitazone were tested in HT-29 and PANC-1 cell lines by MTT growth test (tetrazolium growth assay) for 48 hours post-treatment. Investigation of the relationship between ciglitazone and PPARγ in the context of apoptosis was investigated at the protein level by Western Blot analysis.  \u0000Results: The results obtained reflect the trend in the publication. In the conducted studies, it was observed that at 10 and 20 μM concentration of ciglitazone affects the growth of the investigated cell lines (PANC-1, HT-29). In addition, studies have shown that this drug increases the activity of PPAR-γ receptors and may affect the kinase gene Akt and Bcl-2 through the receptors themselves. \u0000Conclusion: The studies show that treatment of cancer cells 10 μM ciglitazone for a certain time affects the upregulation of anti-apoptotic proteins. What may suggest that certain types of ligand does not result in inhibition of the process of carcinogenesis? Therefore, studies on the effect of PPAR-γ receptors and their ligands on intestinal tumors should be conducted.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128924302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AWARENESS ABOUT VERTEBRAL CANCER IN POSTGRADUATE BIOLOGY STUDENTS","authors":"M. Qadir","doi":"10.53555/eijbps.v4i1.26","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.26","url":null,"abstract":"Vertebral cancer or tumor is a type of cancer which is caused either by formation of tumor in spinal cord and spread in the vertebrae, any type of cancer can proliferate vertebrae, bone cancer can also spread to spine but mostly the vertebral cancer is caused by metastasis of tumor from different parts mainly from breast, prostate, and lungs. A questioner was prepared and awareness among 38 postgraduate biology student was studied it was found that according to the results 56.6% students consider it a viral disease whereas 2% consider it as bacterial and fungal disease, 60.2% consider it as a genetic disease and 40.9% consider it a metabolic disease None of the student suffer from the disease whereas 2.63% student’s relative and family suffer from the disease, according to 23.6%student the disease can be transferred by blood and 35.57% says that it is transferred from parent to offspring’s. According to 65.5% student disease can be cured by medicine, 82.31% agreed that surgery is used for treatment and according to 8.894% student the disease is not curable, hence it was concluded that some of the postgraduate biology students were aware of disease and some have little knowledge.  Key words: vertebral cancer, proliferation, breast cancer, lung cancer.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128390286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PREVALENCE OF MALARIA USING TWO RAPID DIAGNOSTIC TEST KIT IN THE DIAGNOSIS OF MALARIA IN GOMBE STATE, NIGERIA.","authors":"Godwin A.A., Sunday I.D","doi":"10.53555/eijbps.v4i1.27","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.27","url":null,"abstract":"This study aims at evaluating the comparative diagnostic efficiencies of two commercially available kits in relation to microscopy in detecting Plasmodium falciparum HRP-2, a polyhistidine antigen in urine and blood of febrile patients for malaria diagnosis along with blood based RDTs. This was an observational study in which matched blood and urine from  symptomatic patients were tested for malaria using two rapid tests, with microscopy as gold standard in the outpatient clinic of Federal Teaching Hospital Gombe, Gombe state North Eastern Nigeria  were enrolled who presented with fever (≥37.5°C). Thick films were prepared, stained and examined for malaria parasite under the microscope using the oil immersion objective while urine samples as well as blood were taken and immediately tested using RDTs. \u0000With the blood smear microscopy as standard, the disease prevalence was 68.4% and sensitivity for the UMT and SD Bio line (Blood RDT) was 70.1% respectively. The RDT had a specificity of 96.3% as well as the positive and negative predictive values were 97.6% and 59.7% respectively. The rapid diagnostic kits showed moderate level of sensitivity compared with blood smear microscopy as they show considerable acceptable levels in the diagnosis of malaria.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122160025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SYNTHESES AND ANTIMICROBIAL EVALUATION OF BROMINATED IMIDAZOLE AND ITS CO (II), CU(II), MN(II), NI(II), AND ZN(II) COMPLEXES","authors":"E. D. O., Ngochindo R. I., Odokuma, L. O., E. O. C","doi":"10.53555/eijbps.v4i1.23","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.23","url":null,"abstract":"2, 4, 5-tribromoimidazole (tbi) was synthesized and its structure was elucidated using spectroscopic methods. its co(ii), cu(ii), mn(ii), ni(ii), and zn(ii) complexes were synthesized and their antimicrobial activities were evaluated against escherichia coli, bacillus cereus strain cf7, bacillus thuringensis strain eb151, pseudomonas aeruginosa strain 335k55, pseudomonas aeruginosa strain pg1, and candida albicans. these compounds were all inactive to both strains of pseudomonas spp. antimicrobial activities of these compounds is found to be in the order tbi > cu[tbi] > cr[tbi] > ni[tbi] > mn[tbi] > zn[tbi] > co[tbi]. the activity of tbi was reduced upon complexation by all the metal ions used.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122205904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SYNTHESES AND EVALUATION OF THE ANTIMICROBIAL ACTIVITIES OF HALOGENATED IMIDAZOLES AND THEIR MN(II), NI(II), AND ZN(II) COMPLEXES","authors":"Damian Osahon Emwanta, R. Ngochindo, L. Odokuma, O. Eruteya","doi":"10.53555/eijbps.v4i1.24","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.24","url":null,"abstract":"4, 5-Dichloroimidazole (4, 5-DCI) and 2, 4,5-Tribromoimidazole were synthesized, recrystallized from the appropriate solvents and characterized using spectroscopic methods. They were screened for their antimicrobial activities against five bacterial strains namely: Escherichia coli, Bacillus cereus strain CF7, Bacillus thuringensis strain EB151, Pseudomonas aeruginosa strain 335K55, and Pseudomonas aeruginosa strain PG1. These microorganisms were very sensitive to the compounds. Their sensitivities were increased appreciably by the Ni2+ and Mn2+ complexes of 4, 5-dichloroimidazole and reduced by its Zn2+ complex. Their sensitivities towards 2, 4, 5-tribromoimdazole were either greatly reduced or totally lost upon complexation. The differences in these sensitivities are attributed majorly to the halogens as the metal ions are common to both ligands.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116084988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRELIMINARY ASSESSMENT OF BIODIVERSITY / ECOSYSTEMS VULNERABILITY BY CLIMATE CHANGE AND SUGGESTION A SYSTEM OF MITIGATION MEASURES FOR THEM, CASE STUDY: HANOI CITY, VIETNAM","authors":"D. H. Mai, Mai Dinh Yen","doi":"10.53555/eijbps.v4i1.25","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.25","url":null,"abstract":"In the paper, the authors applied the following items in order to assess the vulnerability of ecosystems / biodiversity of Hanoi city caused by climate change and to suggest a system of mitigation measures for them: \u0000 \u0000The concepts on vulnerability, resilience, exposure, sensitivity, adaptation, mitigation according to guidelines of IPCC (2007). \u0000The impacts of climate change on ecosystems/biodiversity according to the results of researches of CBD experts (2009). \u0000Analysis the impacts of the factors of climate change on ecosystems/biodiversity according to the principles of ecological sciences. \u0000The characteristics of all 13 main ecosystems/biodiversity of Hanoi city are research results of the authors and our colleagues. \u0000 \u0000Preliminary assessment of ecosystems/biodiversity vulnerability by climate change of the 13 above main ecosystems/biodiversity of Hanoi city are: \u0000 \u0000Group of ecosystems/biodiversity with high vulnerability: urban ecosystems, rural ecosystems, agricultural ecosystems, grassland ecosystems, land ecosystems yards outside the dyke. \u0000Group of ecosystems/biodiversity with middle vulnerability: nature lake ecosystems, dam ecosystems, wetland ecosystems, stream ecosystems, big river ecosystems. \u0000Group of ecosystems/biodiversity with low vulnerability: cave ecosystems, forest ecosystems. \u0000 \u0000A system of 7 mitigation measures must be applied, such as: to increase the sustainability of biodiversity/ecosystems, to reduce current pressure on biodiversity/ecosystems, to enhance community awareness, to actively implement REDD program.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115107329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}