{"title":"PHYSICO CHEMICAL AND FUNCTIONAL PROPERTIES OF CHICKPEA PROTEIN ISOLATE","authors":"Z. Oo, Thwe Linn Ko, S. Than","doi":"10.53555/eijbps.v3i1.20","DOIUrl":"https://doi.org/10.53555/eijbps.v3i1.20","url":null,"abstract":"The main purpose of this research work was to isolate the most refined form of protein from chickpea for food processing. In this research work, chickpea (Cicer arietinum. L) was collected from Monywa Township, Sagaing Region and nutritional characteristics such as moisture content, ash content, fat content, carbohydrate content, protein content and fiber content were determined. The fat of raw bean flour was removed by bulk soaking in ethanol and also by soxhlet extraction using ethanol as solvent before isolating the protein. In addition, the fiber and starch from defatted chickpea flour was removed by alkaline extraction and acid precipitation method to isolate the protein (isoelectric precipitation). Protein solubility, water and oil absorption capacity, emulsifying capacity and stability, foaming capacity and stability of chickpea protein isolate have been determined. The solubility curve corresponding to the chickpea protein isolate indicated the minimum solubility at pH 4 (protein solubility of 24 %) and maximum solubility at pH 12 (protein solubility of 89 %) respectively. The chickpea protein isolate had water absorption capacity of 1.65±0.12 mL H2O/g protein and oil absorption capacity of 1.72±0.34 mL oil/g. protein. It was found that emulsion stability of isolated chickpea protein was 40.12 ±0.33 %with foaming capacity was 63.64±0.22 %. Isolated chickpea protein improved texture appearance and taste than the lentil flour and thus it can better be used as nutrition and functional ingredients in many food products.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131810152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Damian Osahon Emwanta, R. Ngochindo, L. Odokuma, O. Eruteya
{"title":"EVALUATION OF THE MICROBIAL TOXICITIES OF 4, 5-DICHLOROIMIDAZOLE AND ITS MN (II), NI (II), AND ZN (II) COMPLEXES","authors":"Damian Osahon Emwanta, R. Ngochindo, L. Odokuma, O. Eruteya","doi":"10.53555/eijbps.v4i1.22","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.22","url":null,"abstract":"4, 5-Dichloroimidazole (4,5-DCI) was synthesized, recrystallized from water and characterized using spectroscopic methods. It was screened for its antimicrobial activities against five bacterial strains namely: Escherichia coli, Bacillus cereus strain CF7, Bacillus thuringensis strain EB151, Pseudomonas aeruginosa strain 335K55, and Pseudomonas aeruginosa strain PG1. These microorganisms were very sensitive to the compound. Their sensitivities were increased appreciably by the Ni2+ and Mn2+ complexes of 4,5-dichloroimidazole and reduced by its Zn2+ complex. The sensitivities of these microorganisms towards these compounds were higher than their sensitivities towards Levofloxacin – a reference antibacterial.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121332177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"BACTERIOLOGICAL LOAD ASSESSMENT OF JUICE SOLD IN CAFTERIA AND HOTELS IN ARBA MINCH TOWN, GAMO GOFA, SOUTHERN ETHIOPIA.","authors":"Diriba Leta Weleni, Essayas Naygaro","doi":"10.53555/eijbps.v3i1.19","DOIUrl":"https://doi.org/10.53555/eijbps.v3i1.19","url":null,"abstract":"Fruit juices are the specialized drinks which are rich in vitamins and nutrients that can help prevent common birth defects as well as cancer and also contain enzymes that are essential for digestive process. The study was performed to evaluate the microbiological quality of locally processed juices such as mango and avocado juices from some selected hotels and cafeteria found in Arba Minch town. Four mango and Avocado, total 8 juice samples from two hotels and cafeterias were collected and their microbial load was analyzed in Microbiology laboratory on appropriate media by using serial dilution techniques followed by spread plate techniques. The highest colonies were observed from mango juice sample from cafeteria two and the lowest colony from hotel one are 94 colonies and 0 colony respectively. The mean bacterial count of avocado juice sample was 49500 cfu/ml which was relatively higher than the mean colonies of mango’s juice sample was 15420 cfu/ml. The total mean of bacterial colonies were 63750 cfu/ml from nutrient agar media, 16250 cfu/ml from Manito salt agar and 23250 cfu/ml from Macconkey in case of cafeteria as well as 41250 cfu/ml from nutrient agar media, 22250 cfu/ml from Manito salt agar and 28000 cfu/ml from Macconkey in case of hotels. Their for, juice venders that produce unpasteuralized mango avocado juices would be recommended that preventative measures through food safety control strategies is important.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134235473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"STUDIES ON PHYSICO-CHEMICAL PROPERTIES OF SANGAMNERI GOAT MILK IN VARIOUS SEASONS OF MILKING","authors":"Ashwini Mukhekar, R. Desale, Madhav Potey","doi":"10.53555/eijbps.v3i1.18","DOIUrl":"https://doi.org/10.53555/eijbps.v3i1.18","url":null,"abstract":"The objective of the research work to study the physico-chemical properties of Sangamneri goat milk namely fat, protein, lactose, total ash, titratable acidity, pH and total solids. During the entire study the fresh goat milk samples of Sangamnari goats were analyzed for chemical properties such as fat, protein, lactose, total ash, titratable acidity, pH and total solids also the minor content viz. Fe, Cu, Mn, and Zn of Sangamneri goat milk in summer, rainy and winter seasons, respectively. The data were statistically analyzed by using completely Randomized Design (CRD) with three treatment and six replications. There was significant variation (P<0.05) was observed among the season of milking as regard iron, manganese, copper and zinc content of Sangamneri goat milk. \u0000There was no significant (<0.005) among the seasons of milking in respect of protein, total ash, titratable acidity, pH and total solids. Whereas, significant variation P(<0.005) was observed among the season of milking as regard fat, lactose, iron, manganese, copper and zinc content of sangamneri goat milk. The overall mean values of chemical composition of milk were 5.24 per cent fat, 3.62 per cent protein, 4.06 per cent lactose, 0.76 per cent total ash, 0.128 per cent titratable acidity, 6.42 pH and 13.62 per cent total solids of Sangamneri goat milk. The mean values of fat, lactose content of Sangamneri goat milk significantly increased from summer to winter season.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127294760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afnidar, Udan Kusmawan, F. H. Syarifa, B. Kuswandi
{"title":"A LAB-ON-A-PAPER FOR LOW-COST AND DISPOSABLE DETECTION OF URIC ACID AND BLOOD IN URINE SAMPLE SIMULTANEOUSLY","authors":"Afnidar, Udan Kusmawan, F. H. Syarifa, B. Kuswandi","doi":"10.53555/eijbps.v4i1.21","DOIUrl":"https://doi.org/10.53555/eijbps.v4i1.21","url":null,"abstract":"This work presents a low-cost and disposable paper based microfluidic system for detection of uric acid and blood in urine simultaneously. Detection is achieved by using a colorimetric or visual indicator. Immobilized specific reagents designed for the parameter under consideration act as capture molecules on the surface of the detection zone of a microfluidic system made of paper (cellulose). Fe-(III)tris-(1,10)-phenantroline and Tetramethylbenzidinecumene hydroperoxide and have been immobilized using absorption method for blood and uric acid detection respectively. For blood detection, the detection zone will be change from pale blue to dark blue, due to presence of hemoglobin, which in turn, resulted in oxidized reaction of tetramethylbenzidine by cumene hydroperoxide. While for uric acid detection zone, the color change from yellow to orange/red due to complexation of Fe(III)tris-(1,10)-phenantroline with uric acid in urine sample. The capability of lab on paper for detection of blood and uric acid in urine samples has been demonstrate successfully.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115025540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ASSESSMENT ON BACTERIAL LOAD OF READY TO USE FRUIT JUICES SERVED IN CAFES AND JUICE BARS IN HOSSANA TOWN, SOUTHERN ETHIOP","authors":"Diriba Leta Weleni, Shilimat Abera","doi":"10.53555/eijbps.v2i1.17","DOIUrl":"https://doi.org/10.53555/eijbps.v2i1.17","url":null,"abstract":"Contaminated food and drinks are source of various food borne diseases in human. The study aimed on the assessment of bacterial load of locally prepared fruit juice. A cross sectional study was conducted from January 2015 to May 2015in Hossana town. Out of Twenty four fruit juice samples, eight samples from each of avocado, papaya and mango were collected at two different time intervals accordingly. The pH of original samples was determined before dilution. Streak plate method was used for the growth of bacteria on appropriate media. Structured questionnaire was prepared to assess storage mechanism of fruit, source of fruit, processing and hygienic condition of working environment. Concerning pH there was slight increase in juices that were collected at night. The probable reason may be inappropriate storage of fruit and over dilution of fruit juices. Regarding the bacterial load of fruit juice samples both avocado9 (38.38%) and papaya8 (33.67%) were found to be more contaminated with bacteria than mango 6(28.53%).This may be the water activity, antimicrobial constituents and/or nutrient content. The venders cause subsequent contamination during preparation, handling, by the side of waste disposal system, using water that is not boiled for dilution. Therefore, regular supervision and training about safe processing and handling of fruit juices and hygiene of venders can improve the quality of fresh fruit juices.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117091256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DISTRIBUTION OF ENDOPHYTIC FUNGI IN TECTONA GRANDIS LINN.F","authors":"A. Gorky, Dr. S. Jenifer","doi":"10.53555/eijbps.v2i1.16","DOIUrl":"https://doi.org/10.53555/eijbps.v2i1.16","url":null,"abstract":"Isolation and enumeration of endophytic fungi from leaf samples of Tectona grandis L.f was carried out in Thanjavur District of Tamilnadu India.During the study period, a total of 140 fungi were isolated from PDA medium and Different fungal species namely Alternaria sp.,, Colletotrichum sp.,, Phomopsis sp., Penicillium sp.,, Schizophyllu sp.,, Xylariaceae Sp.,, Schizophyllum Sp., Nigrospora sp., Fusarium sp., The Lower portion of leaf sample was most frequently colonized Penicillium sp, Schizophyllum Sp., Fusarium sp., and Xylariaceae sp,, (15%),Followed by Alternaria sp, Colletotrichum sp, and Phomopsis sp(13.5%), whereas Nigrospora sp., was found only in young leaves.However,from the upper region was most frequently colonized by Alternaria sp (10%)followed by Xylariaceae sp.,, , Colletotrichum sp, Phomopsis sp., (8.5%).%) and Fusarium sp.,( 5.0%) and sterile mycelium(4.5%). There was the significant Differenes in the type of colonization of fungus with reference to reason and location.However,from the upper region was most frequently colonized by Alternaria sp (10%)followed by Xylariaceae sp.,, , Colletotrichum sp,and Phomopsis sp., (8.5%).Whereas at upper portion of leaf segment was mostly similar colonized by Alternaria sp(15%), Colletotrichum sp,and Phomopsis sp.,Fusarium sp., (13.5%).It was noticed in general, that the colonization frequency of some of those fungi, Alternaria sp,Schizophyllum sp, Xylariaceae sp, and sterile mycelium was little higher percentage in lower portion of the leaf. The findings in the present study is also agreement with finding of Bills (1996).who reported large number of fungal species isolated as entophytes from the leaves of this plant sp. The study of entophytic fungus in Ticino grandis suggests that there is a need of larger survey in relation to various seasons to study the diversity existing in this species and their seasonal variability on endophytes colonization. They showed colonization frequency was greater during rainy season than summer and winter.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122215592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INTERACTIVE EFFECTS OF QUANTUM FLUX, TRANSPIRATION RATES AND LEAF STOMATA CONDUCTANCE ON NET CO2 ASSIMILATION RATES OF SAVANNAH GRASSES IN KENYA","authors":"E. Maranga","doi":"10.53555/eijbps.v2i1.15","DOIUrl":"https://doi.org/10.53555/eijbps.v2i1.15","url":null,"abstract":"The current study was conducted to investigate the interactive effects of quantum flux, transpiration rates, and leaf stomata conductance on net CO2 assimilation rates of Panicum maximum beneath Acacia tortilis canopies and disturbed micro sites where these trees were removed. A randomized complete block design was used to measure the treatment effects created by cutting Acacia tortilis trees on CO2 assimilation rates of Panicum maximum. The mean quantum fluxes for the canopy micro sites and disturbed micro sites were 694.8µmol m-2 s1 and 1184.5µmol m-2 s-1, respectively. The average leaf stomata conductance values for Panicum maximum in the exposed and canopy micro sites were 249.6 mmolm-2s-1 and 332.8mmol m-2 s-1 respectively. These average leaf stomata conductance values were significantly different at p≤ 0.05 Transpiration means for Panicum maximum in the exposed micro sites (4.0 mgcm-2s-1) and canopy micro sites (4.2mg cm-2s-1) were not significantly different at P≤ 0.05. Panicum maximum in the disturbed micro sites exhibited significantly lower (p≤0.05) net CO2 assimilation rates (mean of 1.4µmolCO2 m-2 s-1) than the rates measured for the same species (mean of 9µmolCO2 m-2 s-1) in the canopy micro sites. These findings suggest that induced disturbances created by clearing of Acacia tortilis trees in the long term will cause shifts in micro environmental fluxes of energy, water and CO2 exchange with significant implications on herbaceous under storey productivity.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133011687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UNPLANNED URBANISATION AND ENVIRONMENTAL DEGRADATION","authors":"R. C. Paramanik, A. Paramanik","doi":"10.53555/eijbps.v2i1.11","DOIUrl":"https://doi.org/10.53555/eijbps.v2i1.11","url":null,"abstract":"Urbanization is an inevitable phenomenon for the country like Bangladesh. The push and pull factors help to migrate people from rural area to urban area. As a result haphazard and unplanned urbanization create environmental degradation. Survey data reveals that inadequate solid waste disposal services, lack of adequate public water supply, traffic congestion, water logging, air pollution, noise pollution, hill cutting are the main problems in the city area. Concentration of dust as well as SOx, NOx exceeded the allowable limit at the selected points. Groundwater level of Sylhet city has a considerable lowering over the last few decades. It has lowered from 3380 mm below ground in 1982 to 7880 mm below ground. Sylhet is located in highly seismic risk zone but most of the buildings and other structures in this area are constructed without considering earthquake risk. Environmental degradation index was calculated based on economic condition of the country.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115931915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ENVIRONMENTAL DEGRADATION FROM USE AND THROW ATTITUDE OF THE HUMANS","authors":"R. C. Paramanik, A. Paramanik","doi":"10.53555/eijbps.v2i1.10","DOIUrl":"https://doi.org/10.53555/eijbps.v2i1.10","url":null,"abstract":"In this present research paper the challenges in the sustenance of life and a dream of offering our future a better place to live-in is discussed. Environmental degradation is the deterioration of the environment through depletion of resources such as air, water and soil; the destruction of ecosystems and the extinction of wildlife. We have only one EARTH. Our environment is deteriorating through depletion and pollution of resources such as air, water and soil. There are problems at different hierarchy-local, regional, national and global. Bangalore, one of the major centers of outsourcing activity, has grown rapidly both demographically and economically and as a result is facing myriad urban infrastructural and environmental problems. Urban consumption is quite visible in the mushrooming of opulent apartment complexes and upscale shopping malls, hotels, and restaurants. The elite and the poor differ in their consumption patterns and therefore in their impacts on the environment. But, environmental degradation is evident at both ends of the income spectrum- the rich pollute due to the wasteful overuse of resources and the poor degrade the environment by surviving at its expense. With increasing population and income, the lifestyle of urban residents is changing. Urban India is thus becoming a ―throw – away society‖. The tonnage of solid wastes is disposed of each day by our consumer society. This mounting problem is due to the negligence attitude and shopping habits. The burden is on family ill- health and onset of variety epidemics. The Tsunami, earthquakes, high temperature and certain unknown diseases are instances of environmental disturbances.","PeriodicalId":257195,"journal":{"name":"EPH - International Journal of Biological & Pharmaceutical Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128697282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}