{"title":"Genetic Variations in rplBGene Associated with Multidrug Resistance AcinetobacterbaumanniiIsolated from Different Clinical Sources","authors":"A. Hamzah","doi":"10.22401/JNUS.21.3.14","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.14","url":null,"abstract":"In this study, two hundred specimens were collected from patients suffering from urinary tract infection, wound infection, otitis infection and respiratory tract infection (fifty specimens from each infection). After laboratory diagnosis by biochemical tests and confirmation by advance tests (VITEK- 2 Compact system), only twenty isolates were diagnosed as Acinetobacter baumannii (10%). Then sensitivity test was carried out of all these isolates by using twelve antibiotics, all isolates exhibited various levels of resistance to different antibiotics. Then DNA extraction of all twenty isolates followed by amplification of rplB gene aggainst using PCR with specific primers. DNA sequencing of all isolates was done, then alignmented sequences in NCBI and drew phylogenetic tree by using Geneious 9 software among locally isolates alone and then between them and high identity globally identified registred isolates in Gene Bank. Distribution of locally isolates in phylogenetic tree showed three different groups. The phylogenetic tree showed there were eight locally isolates differed from the standard isolates. From these locally isolates, one isolate (AE_12) was documented in NCBI under accession number (LOCUS KY818058) of nucleotides sequence and protein ID \"ARV90996.1\".","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"305 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121156459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. K. Hassan, Hasan Fadhil AL-Rubai, Hadi Hassan AL-Shamary
{"title":"The Kinetic Model for Decolorization of Commercial Reactive Red 120 Azo Dye Aqueous Solution by the Fenton Process and Study the Effect of Inorganic Salts","authors":"A. K. Hassan, Hasan Fadhil AL-Rubai, Hadi Hassan AL-Shamary","doi":"10.22401/JNUS.21.3.09","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.09","url":null,"abstract":"The degradation of a commercial azo dye Reactive Red 120 (RR120) in synthetic aqueous solution using Fenton's oxidation has been studied. The influence of different reaction parameters such as pH, hydrogen peroxide, ferrous sulfate, and the RR 120 concentration on the oxidative degradation of RR 120 have been appraised. The optimal reaction conditions were determined and it was found to be pH = 3.50, [H2O2] = 1.1×10-3 M, [Fe2+] = 1.0×10-4 M for [RR 120] = 7.5×10-5 M. Under optimal conditions, 96.0% decolorization efficiency of dye in aqueous solution was achieved after 15 min of reaction. The effect of azo bond loading (Lazo bond), from 0.25 to 1.0, and pH values from 2.5 to 5.0 were estimated on RR 120 color removal kinetic rates. A correlation between the kinetic of the color removal rates (ln k2) versus Lazo bond was carried out at the different pH levels. The color removal rate increased linearly with decreasing Lazo bond, in the order of pH: 3.5 > 5.0 > 2.5. All the experimental data were analyzed using the first and second-order kinetic models. The second-order provides the best correlation of the data. 67% Chemical Oxygen Demand (COD) removal efficiency of the RR 120 were achieved after 15 min of reaction by fixing the initial H2O2/Fe2+ molar ratio, COD loading factor (LCOD), and pH at 11, 0.25 and 3.5, respectively. Also, the effects of various inorganic anions (such as Cl−, SO42-, CO32-, etc.) on the oxidation efficiency of Fenton were studied. This study can benefit planners who deal with contaminated textile wastewater using chemical treatment by advanced oxidation technologies.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121144947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equilibrium and Thermodynamic Studies of Removal of Two Dyes from Aqueous Solutions using Low Cost Adsorbent","authors":"Z. Hussain","doi":"10.22401/JNUS.21.3.03","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.03","url":null,"abstract":"The capability of the low cost materials viz. Dried Mint Leaves (DML) to eliminate two cationic dyes, Methylene Blue (MB) and Safranin-O (SF-O) from an aquatic solutions was studied by the technique of batch mode adsorption process. Influence of contact time, initial dye concentration (mg/L), adsorbent amount (g/L) and temperature was investigated. The equilibrium and thermodynamic peculiarities of the dyes abscession were too inspected. The empirical data were found to comply the Langmuir and Freundlich models. Also Dubinin-Radushkevich model show that the process was physisorption. The negative free energy pointed to that the adsorption processes were spontaneously feasible. The process of adsorption has been existed to be exothermic in nature. The abscession of MB was found to be more spontaneous and feasible than the removal of SF-O on the adsorbent.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127964579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Consequences of PCBs and BPA Resulting from Terrorist Operations in Three Regions of Baghdad on Thyroid Function","authors":"Alaa H. Jawad, S. Kadhim, Z. H. Al-Qaisi","doi":"10.22401/JNUS.21.3.11","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.11","url":null,"abstract":"Persistent organic pollutants polychlorinated biphenyls (PCBs) and bisphenol-A (BPA) are widely known of being toxicants. Exposure to a variety of dangerous toxic effects has been attached in all humans .in this paper the effect of terrorist operation on thyroid gland functions in a sample of Iraqi individual (AL-karradah and Abo-Gharib) comparison with Al-jadria rejoin as (control). The correlation between sera thyroid hormones thyroxine T4, triiodothyronine T3, Thyrotropin TSH and the levels of polychlorinated biphenyls (PCBs) and bisphenol-A (BPA) have been measured. 75 volunteers from three Iraqi areas were included in this study. Their ages ranged between (15-65) years. Our examination proposes an inverse relationship between BPA presentation and thyroid hormones. In this manner, increased persistent organic pollutants exposure might be a factor in the causes of hypothyroidism and thyroid gland function. The result of this study showed that the effect of persistent organic pollutants polychlorinated biphenyls (PCBs) and bisphenol-A (BPA) on thyroid hormones, and the effect of military and terrorist operations on the thyroid gland. In the end we concluded that persistent organic pollutants resulting from the explosions and terrorist operations in the area of (Al-karadah, Abo-Gharib) high compared with the control (Al-jadria) as the lowest percentage of pollution, this leads to many diseases on the health of the human body and also the effect of persistent organic pollutants on the thyroid gland and its negative effect on thyroid hormones and cause thyroid disease.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"134 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131306874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental and Theoretical Study of 4-Methylaminoantipyrine with Divalent Metal Ions","authors":"F. Ibrahim","doi":"10.22401/JNUS.21.3.08","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.08","url":null,"abstract":"Co2+, Ni2+, Cu2+ complexes with 4-methylaminoantipyrine (MAP) were synthesis and characterized by IR, UV-Vis., thermal analysis, CHNO-S analysis, magnetic susceptibility, conductivity measurements and this work includes a theoretical study of MAP complexes where it was done by the program of hyperchem8.0.7 using semi-empirical calculations. The PM3 method at 298 K used to calculate geometric properties, binding energy (ΔEb), heat of formation (ΔH○f), total energy (ΔEtot.), ultraviolet and vibrational data of the MAP complexes. The comparing of experimental data with theoretical data gave good results, so the square planar geometry suggested for complexes.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124769999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Reflections Removal Using K-Means Clustering","authors":"Y. Ali, Maisa S. Mohsen","doi":"10.22401/JNUS.21.3.19","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.19","url":null,"abstract":"The reflection generated from the semi reflectors (glass windows) affects the algorithm efficiency of the computer vision. It is a naturally hard problem to separate a single overlapped image into a reflection image and a transmission image, which is a kind of blind separation problems. This paper presents an automatic algorithm to separate the reflected and the transmitted components of a single superimposed image by clustering mixture pixels. Various examples are tested to validate the effectiveness of the proposal algorithm. Experimental results proved that the proposed algorithm can produce acceptable results.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"17 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131454029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yasir B. Fadhil, Khuldood W. Al-Sammarraie, Nedhal Abdul Mohaimen
{"title":"Cytotoxic and Cell Cycle Arrest of Total Alkaloids Extracted from Chelidonium MajusAgainst Human Colon Cancer (HCT-116).","authors":"Yasir B. Fadhil, Khuldood W. Al-Sammarraie, Nedhal Abdul Mohaimen","doi":"10.22401/JNUS.21.3.16","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.16","url":null,"abstract":"Chelidonium majus, commonly known as Greater Celandine is a plant widely distributed in nature the plant been used in various traditional system of medicine to treat various disorders. Antitumor activity of total extracted alkaloid of this plant have been studied, there are few studies that examine the properties of the total extracted alkaloids. Thus the objective of this project is to investigate cytotoxic effects of total alkaloid extracted from C. majus against Human colon cancer cell line. Powdered plant material was extracted with methanol by using Soxhlet installation. The extract subjected for acid-base extraction and purification with chloroform to obtain total alkaloids. Cytotoxicity effect of total alkaloids was estimated using MTT-assay in addition to the determination of apoptosis/necrosis FITC Annexin V /PI assay and Cell Cycle Analysis by Pi Staining. It was found that Chelidonium majus contain high amount of total alkaloids where the yield of total extracted alkaloids was about (2.146 %) of total dried weight. The total extracted alkaloids from C. majus showed high significant cytotoxicity effect on (HCT-116) cell lines which was in a dose dependent way, where the IC50 was (67.43 µg/ml). Annexin V-FITC assay showed that total alkaloids from C. majus is strong inducer of apoptosis in HCT-116 cell line. While, the cell cycle arrest evaluation showed that the extract arrests cell cycle progression by significantly restricting cells in different phases (G1, S, G2/M) in a dose dependent way. It is concluded that the plant contains a large quantity of total alkaloids. The total extracted alkaloids showed cytotoxic activity against colorectal cancer (HCT-116) cell line which was in dose dependent manner indicating that the extract could be used in cancer therapy.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133666074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Incorporation of Ions into Giant Molybdenum-oxide Cluster as a General Phenomenon","authors":"Al-Ameen Bariz OmarAli","doi":"10.22401/JNUS.21.3.01","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.01","url":null,"abstract":"In spite of the integration and concomitant release of all 16 {Mo1} (necessary for ions Recently, the integration of different kinds of matter into giant wheel-shaped molybdenum-oxide clusters has been investigated with so-called chemical adaptability phenomenon. This refers to the integration of a large number of appropriate cations and anions even of the large “salt like” {M(SO4)}16 rings (M = K+ or NH4+) based on the cooperative/synergetic activation of silent receptor sites. An even more remarkable consequence of the flexibility behavior of the molecular giant wheel-shaped molybdenum-oxides of the type {Mo176} and {Mo154}: the possibility of the integration of Cu2+ as tetrahedrally coordinated divalent cations at the same wheel area. In this study and in order to make the phenomenon as a general, the incorporation of ions into giant molybdenum-oxide cluster was expanded to involve the integration of some other cations (Fe2+, Co2+, Ni2+ or Zn2+) or salt like ring{Tl(SO4)}16. Uptake and almost half of the 16 {Mo2} units the parent ring structure is maintained in the following resulting hybrids {(Mo146) (K(SO4))16} 1compound, {(Mo146) (NH4(SO4))16} 2 compound and {(Mo146) (Tl(SO4))16} 3. Moreover, the integrating of a tetrahedrally coordinated divalent cation like Fe2+, Co2+, Ni2+ or Zn2+ in addition to Cu2+, at the same wheel area (after releasing six {Mo2}-type building blocks of the parent wheel-shaped cluster) produced hybrid compounds with the general formula: [H4MIIn MoV28MoVI114O432(H2O)58]-26+2(n-5) (where MII= Fe2+, Co2+, Ni2+ or Cu2+, n=5, but with Zn2+, n=6) compounds (4–8). The result corresponds to the extreme flexibility of molybdenum-oxide building block linking resulting in the formation of a huge variety of structures from “molybdate” containing solutions (a unique molybdenum-oxide diversity phenomenon). The prepared compounds were characterized by CHN, Cit.Tit for the determination of the number of the MoV centers, spectroscopic methods (IR and UV-Vis) and X-ray crystallography (determination of the characteristic unit cell dimensions).","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127868385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Comparison of Corner Feature Detectors for Video Abrupt Shot Detection","authors":"M. Abdulmunem, Eman Hato","doi":"10.22401/JNUS.21.3.20","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.20","url":null,"abstract":"Comparison of feature detectors and evaluation of their performance is very important in computer vision. A new algorithm is proposed in this paper to compare the performance of four corner feature detectors based on abrupt shot boundary detection. The proposed algorithm consists of two stages: feature vectors generation where corner detector for all video frames is computed to obtain the descriptor feature vectors, and features matching where the number of matching features between two successive frames is calculated. The corner feature detectors used in this paper are BRISK, Harries, MinEigen, and FAST. Experimental results indicate that the proposed algorithm using MinEigen features detector provides better performance than other features detectors where the average value of recall, precision, and F measure is 0.99083, 0.98808, and 0.98875 for selected testing videos respectively. The results also show that the FAST is superior to others feature detectors when considering execution time.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115849797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"1, 2(2, 2-dihydroxy Benzelidenamine) Phenyl Complexes as Corrosion Inhibitor for Carbon Steel in Hydrochloric Acid","authors":"Dina A. Najeeb, T. Salman","doi":"10.22401/JNUS.21.3.02","DOIUrl":"https://doi.org/10.22401/JNUS.21.3.02","url":null,"abstract":"The corrosion inhibition on carbon steel in 1MHCl by 1, 2(2, 2-dihydroxy benzelidenamine) phenyl and its complexes was studied at different concentrations by \"weight loss method\". Data mark, for the compound shows good performance. These measurements show that the inhibition efficiency obtained by these complexes increased by increasing their concentration. The inhibition efficiency follow the order Co>Ni>Cu. The activation and adsorption thermodynamic parameters were determined and discussed. The surface coverage (Ɵ) of the inhibitors conform \"Langmuir adsorption isotherm\". (Morphology) of the carbon steel alloy has been examine using \"scanning electron microscopy\" in existence and inexistence of inhibitor.","PeriodicalId":255225,"journal":{"name":"Journal of Al-Nahrain University of Science","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123438083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}