Biochimie最新文献

{"title":"Dietary advanced glycation end products (AGEs): A modifiable risk factor in the prevention of chronic diseases associated with aging?","authors":"Felipe Ávila ,&nbsp;Nadia Cruz ,&nbsp;Ma Angélica González ,&nbsp;Eduardo Fuentes ,&nbsp;Sergio Wehinger ,&nbsp;Mariane Lutz","doi":"10.1016/j.biochi.2025.06.001","DOIUrl":"10.1016/j.biochi.2025.06.001","url":null,"abstract":"<div><div>Advanced glycation end products (AGEs) are a heterogeneous group of compounds formed during the advanced stages of the Maillard reaction through non-enzymatic reactions, occurring mainly between reducing sugars or their oxidation metabolites and amino groups in proteins, lipids, and nucleic acids. These compounds can be endogenously formed, particularly under hyperglycemic conditions. In addition, AGEs are also produced exogenously during the thermal processing of foods, contributing to the dietary intake of these compounds. The accumulation of AGEs in body tissues has been associated with aging and the pathogenesis of various non-communicable diseases. Dietary intake of AGEs contributes significantly to their systemic burden. Consequently, reducing the intake of dietary AGEs has been proposed as a modifiable risk factor for the prevention of chronic, age-related diseases. This review examines and discusses current evidence on the molecular mechanisms by which dietary and endogenous AGEs contribute to cellular senescence and the progression of prevalent age-associated pathologies, including diabetes, cardiovascular diseases, musculoskeletal disorders, and neurodegeneration.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 80-92"},"PeriodicalIF":3.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated physiologic and proteomic analysis of Antarctic yeast Rhodotorula mucilaginosa AN5 in response to Cd stress at low concentration","authors":"Guangfeng Kan ,&nbsp;Yuexin Zhang ,&nbsp;huixing Sheng ,&nbsp;Ke Song ,&nbsp;Yun Ju ,&nbsp;Wanli Zong ,&nbsp;Yanxiao Jiang ,&nbsp;Yingying Wang ,&nbsp;Cuijuan Shi","doi":"10.1016/j.biochi.2025.05.011","DOIUrl":"10.1016/j.biochi.2025.05.011","url":null,"abstract":"<div><div>In Antarctica, sea ice microorganisms are subjected to principal abiotic environmental stresses, such as low temperature, strong UV radiation and high salinity, but increasingly serious heavy metal pollution might has more grievous implication there. Herein, morphological, physiological and isobaric tag for relative and absolute quantification (iTRAQ)-based proteomic analyses were performed to anatomize the heavy metal adaptation mechanism of <em>Rhodotorula mucilaginosa</em> AN5, a type of basidiomycetous yeast strain isolated from Antarctic sea ice. Morphological observation showed that yeast AN5 could partly alleviate cadmium stress by the increase of cell size. KEGG functional enrichment along with physiological analysis showed that heavy metal tolerance of sea ice yeast might prominently be related to unsaturated fatty acids biosynthesis regulated by peroxisome proliferators-activated receptor (PPAR) signaling pathway, which is the first report in non-animal field. In addition, redox equilibrium, substances metabolism and energy generation also played key roles for yeast acclimation upon Cd stress. In general, Antarctic yeast could cope with heavy metal mainly by morphological alteration, ROS-scavenging capacity improvement and unsaturated fatty acids biosynthesis. This research provided particular insight into the tolerance mechanisms of Antarctic sea ice yeast in response to heavy metal stress.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 68-79"},"PeriodicalIF":3.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144201044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ontogenetic variation in coagulotoxic activity and antivenom efficacy of Central American pit viper venoms (Cerrophidion, Metlapilcoatlus, Ophryacus and Porthidium)","authors":"Lee Jones ,&nbsp;Edgar Neri-Castro ,&nbsp;Uri O. García-Vázquez ,&nbsp;Bryan G. Fry","doi":"10.1016/j.biochi.2025.05.009","DOIUrl":"10.1016/j.biochi.2025.05.009","url":null,"abstract":"<div><div>Ontogenetic variation in venom activity and composition has been documented across several pit viper genera, but these toxicological variations are poorly understood in <em>Cerrophidion</em>, <em>Metlapilcoatlus</em>, <em>Ophryacus</em>, and <em>Porthidum</em>. This study investigated ontogenetic changes in coagulotoxic venom activity across adult and juvenile specimens of <em>Cerrophidion petlalcalensis</em>, <em>Metlapilcoatlus olmec</em>, <em>M. nummifer</em>, <em>Ophryacus undulatus</em>, <em>Porthidium ophryomegas,</em> and <em>P. yucatanicum</em>. Adult <em>C. petlalcalensis</em>, <em>M. olmec</em>, and <em>M. nummifer</em> displayed potent thrombin-like activity, resulting in a pseudo-procoagulant action whereby the venoms directly acted upon fibrinogen to produce weak, unstable fibrin clots that rapidly break down, thereby contributing to anticoagulant pathophysiological effects by depleting fibrinogen levels. In contrast, their juvenile counterparts showed either weaker or no such activity. <em>Ophryacus undulatus</em> displayed an ontogenetic shift in anticoagulant targets, where adults inhibited clotting factors and juveniles did not. The anticoagulant target for juvenile <em>O. undulatus</em> remains unknown, with this study ruling out fibrinogen degradation, factor inhibition and phospholipid degradation. Both adult and juvenile <em>P. ophryomegas</em> and <em>P. yucatanicum</em> exhibited potent anticoagulant effects through fibrinogen degradation and factor inhibition. These species exhibited previously undocumented factor VIIa inhibition, a mechanism not previously reported in viperid venoms. The regionally available PoliVal-ICP polyvalent antivenom effectively cross-neutralized the pseudo-procoagulant activities of <em>Cerrophidion</em> and <em>Metlapilcoatlus</em>, across both life stages tested. These findings reveal significant ontogenetic variation in venom activity and antivenom efficacy, with implications for both clinical management of snakebite and our understanding of venom evolution in these species.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 49-58"},"PeriodicalIF":3.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144183532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitric oxide initiates oxidative independent apoptosis-like death in Candida albicans by lupeol","authors":"Ji Hyun Park ,&nbsp;Dong Gun Lee","doi":"10.1016/j.biochi.2025.05.007","DOIUrl":"10.1016/j.biochi.2025.05.007","url":null,"abstract":"<div><div>Lupeol, a dietary triterpene-type phytochemical flavonoid, was investigated for its mode of action in <em>Candida albicans</em> by assessing reactive species generation. While increased intracellular nitric oxide (NO) levels were detected, negligible levels were observed for other reactive oxygen species (ROS) and peroxynitrite(ONOO⁻). The major NO scavenger <span>l</span>-NAME was applied in further experiments to determine whether NO was responsible for the observed processes. DNA damage, including fragmentation and condensation, occurred when the NO concentration increased. Additionally, G1 to S phase cell cycle arrest was induced, followed by mitochondrial dysfunction, including mitochondria mass variation and membrane depolarization. Consequently, typical apoptotic hallmarks such as caspase activation and phosphatidyl serine exposure were monitored. Thus, this study demonstrates that NO can exclusively exert lethal damage without the contribution of highly cytotoxic ROS. In conclusion, lupeol triggers downstream effects in fungal cells following DNA damage, mitochondrial dysfunction, cell cycle arrest, and caspase activation in response to apoptosis-like cell death under NO influence.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 29-38"},"PeriodicalIF":3.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of a new affiprobe that targets HER 3 positive cells using protein engineering","authors":"Farnaz Karbasi ,&nbsp;Rahman Emamzadeh ,&nbsp;Mahboobeh Nazari","doi":"10.1016/j.biochi.2025.05.008","DOIUrl":"10.1016/j.biochi.2025.05.008","url":null,"abstract":"<div><div>Engineered affiprobes that detect a specific target by generating a target-dependent signal, play an important role in biomedical sciences such as breast cancer monitoring. In this study, a new bioluminescence affiprobe consisting of an HER3-binding affibody and a bioluminescent protein, C-SRLc8, a thermostable variant of <em>Renilla</em> luciferase has been developed. The bioluminescent affibody, called C-SRLuc8‒Z₀₈₆₉₉ was expressed in the <em>Escherichia coli</em> and purified by affinity chromatography. The suitability of the new affiprobe to detected HER3 positive cells was evaluated. It was found that the new affiprobe allowed the detection of HER3 expressing cells with a detection limit of 14578 cells per 200 μl assay volume and a working range of 14000–50000 cells. The new affiprobe can be used for detecting, studying and monitoring the HER3-expressing cells using a luminometer in fast and inexpensive assays.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 59-67"},"PeriodicalIF":3.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and kinetic profiling of Leishmania braziliensis trypanothione reductase: A molecular model for the development of targeted therapies","authors":"Ana F. Gómez Garay ,&nbsp;Jorge J. Alfonso ,&nbsp;Aleff F. Francisco ,&nbsp;Erika C.S. Araújo ,&nbsp;Marcos R.M. Fontes ,&nbsp;Carlos A.H. Fernandes ,&nbsp;Soraya S. Pereira ,&nbsp;Anderson M. Kayano ,&nbsp;Leonardo A. Calderon ,&nbsp;Jamile M. Macedo ,&nbsp;Mateus F. Souza ,&nbsp;Rudson J. Holanda ,&nbsp;Juliana C. Sobrinho ,&nbsp;Andreimar M. Soares","doi":"10.1016/j.biochi.2025.05.006","DOIUrl":"10.1016/j.biochi.2025.05.006","url":null,"abstract":"<div><div>This study explores the recombinant protein expression, purification, and characterization of <em>Leishmania braziliensis</em> Trypanothione Reductase (<em>Lb</em>TR), an essential enzyme implicated in cutaneous leishmaniasis. Using <em>E. coli</em> as the host organism, the synthetic gene encoding <em>Lb</em>TR was successfully expressed and subsequently purified using Immobilized Metal Affinity Chromatography (IMAC) yielding 20 mg/L of highly pure <em>Lb</em>TR, verified by SDS-PAGE and isoelectric focusing. Comprehensive biochemical analyses were conducted to determine the recombinant enzyme's kinetic properties and structural features. Enzymatic assays revealed that <em>Lb</em>TR efficiently reduces its natural substrate, following Michaelis-Menten kinetics. Structural characterization, including dynamic light scattering and fluorescence spectroscopy, confirmed the protein's stability and homogeneity in solution under varying temperatures. Circular dichroism analysis corroborated the presence of significant α-helical and β-sheet content, aligning with the structural model generated with AlphaFold. Molecular dynamics simulations over 1 μs were employed to investigate the conformational dynamics of <em>Lb</em>TR in its native homodimeric form, complexed with essential cofactors and substrates. The results from these simulations offer valuable insights into the enzyme's structural behavior and catalytic mechanism, underscoring its potential as a target for therapeutic development against leishmaniasis.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 14-28"},"PeriodicalIF":3.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144103284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside front cover-EDB","authors":"","doi":"10.1016/S0300-9084(25)00087-2","DOIUrl":"10.1016/S0300-9084(25)00087-2","url":null,"abstract":"","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"233 ","pages":"Page IFC"},"PeriodicalIF":3.3,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Caffeine modulates immunoproteasome activity and content in colorectal adenocarcinoma cells","authors":"Alexander Burov ,&nbsp;Alexander Rezvykh ,&nbsp;Valeria Vedernikova ,&nbsp;Alexey Belogurov Jr. ,&nbsp;Vladimir Prassolov ,&nbsp;Pavel Spirin ,&nbsp;Sergey Funikov ,&nbsp;Alexey Morozov ,&nbsp;Vadim Karpov","doi":"10.1016/j.biochi.2025.05.004","DOIUrl":"10.1016/j.biochi.2025.05.004","url":null,"abstract":"<div><div>Proteasomes hydrolyze most intracellular proteins. Immunoproteasome is a form of proteasome implicated in inflammation, cancer and autoimmune diseases. Modulation of immunoproteasome activity is a promising approach against several pathologies. Using previously obtained reporter colorectal cancer cell lines, we tested how commonly used compounds including ibuprofen, acetylsalicylic acid, vitamin C, caffeine and others, affect immunoproteasome expression. Flow cytometry, qPCR and Western blot were used to evaluate immunoproteasome subunit expression. Proteasome activity was tested using fluorogenic substrates and the activity-based probe. Transcriptome analysis was performed to identify patterns of gene expression changes. Interestingly, caffeine was the only drug that stimulated modest reduction in quantity of immunoproteasomes. The effect of caffeine varied between cell lines and was stronger as a result of prolonged treatment. The reduction of immunoproteasome content in cells coincided with decreased expression of immunoproteasome subunits, genes encoding the Nrf3 transcription factor and a PAC4 proteasome assembly chaperone, as well as the reduced levels of oxidative stress. Caffeine did not affect the degradation of immunoproteasomes by autophagy. Obtained results uncover novel biological effects of caffeine, our data might help to optimize existing and develop new strategies for the treatment of colorectal cancer and several autoimmune diseases.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"235 ","pages":"Pages 1-13"},"PeriodicalIF":3.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144045938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the molecular basis of hygienic behavior in Apis cerana indica through antennal proteomics","authors":"Ramkumar Haran ,&nbsp;Chakkarai Sathyaseelan ,&nbsp;Ettiappan Sumathi ,&nbsp;Sundaravadivel Sathiya Priya ,&nbsp;Muthusamy Gayathri ,&nbsp;Ravichandran Prathiksha ,&nbsp;Ganeshan Shandeep ,&nbsp;Mannu Jayakanthan","doi":"10.1016/j.biochi.2025.05.002","DOIUrl":"10.1016/j.biochi.2025.05.002","url":null,"abstract":"<div><div>Hygienic behavior in honey bees, particularly <em>Apis cerana indica</em>, is essential for the health of the colony as it helps reduce the impact of diseases and parasites. Despite its importance, the underlying molecular mechanisms remain inadequately characterized. Using a label-free quantitative proteomics method, this study investigates the antennal proteome associated with hygienic behavior. We employed Principal Component Analysis, Partial Least Squares Discriminant Analysis, and RT-qPCR to identify significant proteins that are involved in this behavior. Our analysis identified 408 proteins in colonies demonstrating high hygienic behavior and 419 proteins in those with low hygienic behavior, with 219 proteins showing significant differences in abundance. Notably, several odorant-binding proteins were upregulated in high-hygiene colonies. Furthermore, pathway enrichment analysis revealed that RNA transport and various signaling pathways are involved in this behavioral trait. The protein-protein interaction analysis illustrated substantial clustering among the odorant-binding proteins, underscoring their critical role in the mechanisms underlying hygienic behavior. This research enhances our understanding of the molecular basis of hygienic behavior in <em>Apis cerana indica</em>, positioning odorant-binding proteins as potential biomarkers for further studies that aim at improving colony health and resilience against pests and diseases.</div></div>","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"234 ","pages":"Pages 110-119"},"PeriodicalIF":3.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inside front cover-EDB","authors":"","doi":"10.1016/S0300-9084(25)00070-7","DOIUrl":"10.1016/S0300-9084(25)00070-7","url":null,"abstract":"","PeriodicalId":251,"journal":{"name":"Biochimie","volume":"232 ","pages":"Page IFC"},"PeriodicalIF":3.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}