ACS Sensors最新文献

{"title":"","authors":"Hao Zhou, Mingjun Shi, Wenhao Yang, Ning Song and Xiliang Luo*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"l-Lactate Oxidase-Based Biosensor Enables Quasi-Calibration-Free Detection of l-Lactate in Sweat of Acidic to Neutral pH.","authors":"Kosuke Ike, Kousuke Muto, Takahiro Hioki, Noya Loew, Isao Shitanda, Masafumi Takesue, Mitsuyoshi Okuda","doi":"10.1021/acssensors.5c01238","DOIUrl":"10.1021/acssensors.5c01238","url":null,"abstract":"<p><p>l-lactate biosensing has attracted attention in recent years in sports, medicine, and nursing care fields as well as in food manufacturing and biotechnology industries. In particular, l-lactate in human sweat, a biological indicator that can be collected noninvasively, has driven rapid progress in the research and development of sensing technology, positioning sweat as a new target to replace blood and interstitial fluid. The key to l-lactate sensing in human sweat, which contains various biological components, is using l-lactate oxidase (LOX) as a recognition element. l-lactate can be specifically, continuously, and quantitatively measured using this enzyme electrode. However, as conventional LOX is affected by acidic pH, biosensors must be calibrated for each individual for accurate l-lactate quantification owing to individual differences in sweat pH. Furthermore, fluctuations in sweat pH during exercise lead to inaccuracies in the detected l-lactate levels. Therefore, identifying LOX active in acidic pH is crucial. Here, we report a novel LOX with acidic pH tolerance and a technology that enables constant detection of l-lactate levels in acidic to neutral pH sweat. Phylogenetic analysis of α-hydroxy acid oxidase in a public protein database, with the evaluation of heterologously expressed enzymes, revealed the existence of a novel LOX with better acidic pH tolerance compared to that observed with conventional LOX. Furthermore, applying the novel LOX to a paper electrode screen-printed with MgO-templated carbon enhanced the l-lactate response at acidic pH compared to that observed with conventional enzyme electrodes while maintaining a pH-independent response to l-lactate. Overall, biosensors utilizing this novel LOX will be quasi-calibration-free, by eliminating the need for adjusting the calibration according to changes in pH. Thus, our findings contribute to expanding the use of l-lactate biosensors targeting sweat and accelerating their societal application.</p>","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":" ","pages":"4707-4716"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210267/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Huihui Chen,&nbsp;Yuhan He,&nbsp;Qijie Wang,&nbsp;Xinyao Yi* and Jianxiu Wang*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Xiaojun Liu,&nbsp;Xiaoyu Chen,&nbsp;Yao Xiao,&nbsp;Ziming Yang,&nbsp;Yong Xu,&nbsp;Andrew P. Ault and Xiangqun Zeng*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.4c03564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Tong Zhai,&nbsp;Wen-Tao Gu,&nbsp;Miao Yu,&nbsp;Yu-Di Shen,&nbsp;Jing-Min Liu* and Shuo Wang*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c01325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Kunru Li,&nbsp;Shuo Qian,&nbsp;Yangyanhao Guo,&nbsp;Jinyuan Liu,&nbsp;Wei Chen,&nbsp;Ruoyang Zhang,&nbsp;Hu Liu,&nbsp;Xiaojuan Hou,&nbsp;Jian He* and Xiujian Chou,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00824","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Li Liang*,&nbsp;Jin Du,&nbsp;Wang Zhang,&nbsp;Heyun Zhang,&nbsp;Yifan Wang,&nbsp;Minhui Liang,&nbsp;Feng Liao,&nbsp;Jianping Shi,&nbsp;Joel K.W. Yang,&nbsp;Zewen Zuo* and Ye Ai*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00294","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Hongqiang Wang,&nbsp;Jiayu Sun,&nbsp;Qingqing Zou,&nbsp;Bin Du,&nbsp;Hui Liu,&nbsp;Yanan Luan,&nbsp;Xin Wang,&nbsp;Xiaohai Yang,&nbsp;Qing Wang* and Kemin Wang,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Kamrul Hassan*,&nbsp;Anh Tuan Trong Tran,&nbsp;M. A. Jalil,&nbsp;Tran Thanh Tung,&nbsp;Md Julker Nine and Dusan Losic*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.4c03281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Tao Zhang,&nbsp;Xingyuan Xu,&nbsp;Xiaotong Li,&nbsp;Chuanjie Yao,&nbsp;Jinkun Chen,&nbsp;Mingqiang Li,&nbsp;Yu Tao,&nbsp;Wenhao Xia,&nbsp;Yan Li,&nbsp;Yunuo Wang,&nbsp;Hui-Jiuan Chen*,&nbsp;Jing Liu* and Xi Xie*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"10 6","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":8.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acssensors.5c00753","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}