Acta medica Eurasica最新文献

{"title":"DEVELOPMENT OF THE METHODOLOGY FOR STUDYING THE SOCIAL ATTENTION OF CHILDREN WITH AUTISM SPECTRUM DISORDERS BY THE EYE TRACKING METHOD (PRELIMINARY RESULTS)","authors":"Alexey N. Kuznetsov, A. Belova, M. Volovik, O. Vorob’eva, Maria E. Khalak","doi":"10.47026/2413-4864-2021-2-17-27","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-17-27","url":null,"abstract":"Development of social skills is an essential condition for the habilitation of children suffering from autism spectrum disorders (ASD). The method of eye tracking (ET) opens up great opportunities for studying social attention in this contingent of people with disabilities. The aim of the study is to form a set of stimulus materials for dynamic ET-testing of eye movement patterns that are sensitive to changes in the level of social attention in preschool-age children with ASD. The selection of stimulus content included the definition of requirements for the quality of the images presented, storage and encoding of stimuli, the development of the procedure for presenting stimuli and the methodology for processing the ET-data. The stimulus material was tested on a group of healthy adults. The developed approach to the formation of standardized stimulus content for testing the social attention of children with ASD by the eye tracking method takes into account the level of children's attention to images, the degree of differences in the results of ET obtained in children of typical development and in children with ASD, as well as the intensity of the dynamics in ET indicators in improvement of clinical indicators in children with ASD.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115789756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TONSILOGENIC AMYLOIDOSIS","authors":"V. A. Kozlov, S. Sapozhnikov, P. B. Karyshev, Alena I. Fufaeva, V. Y. Aleksandrova, Yu. V. Vasilyeva","doi":"10.47026/2413-4864-2021-2-7-16","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-7-16","url":null,"abstract":"In connection with the report that amyloid is a functional antimicrobial substance, possibly a part of innate immunity, we suggested that amyloid may form in the palatine tonsils in chronic tonsillitis. Thirty-two patients aged 19 to 52 years underwent surgical treatment for clinically proven chronic tonsillitis. The aim of the study was to detect the fact of amyloid tonsillar lesions formation in chronic tonsillitis. We performed histological examination of tonsillo-ectomized material fixed in 10% formalin solution and filled in paraffin on 4-micron histological sections: 1) according to the conventional technique, stained with hematoxylin-eosin, 2) Congo red staining and 3) using our own original trichromatic method (two-hour incubation of paraffin sections in acidin-pepsin, sequential staining with picro-fuchsin according to Van Gieson, Сaracсi hematoxylin, Congo red according to H. H. Benhold, picro-indigocarmine). All 32 patients showed a clinical presentation of chronic tonsillitis without exacerbation with stroma sclerosis and atrophy of the lymphatic glandular system. Local amyloidosis of the tonsils was detected in 10 out of 32 cases. As a result of trichromatic staining, unchanged connective tissue fibers stained green, changed tissues (hyalinosis or amyloidosis) stained in various shades from yellow, yellow-orange to red, in addition, lymphocytes, neutrophils and mast cells were clearly detected in the tissues of the amygdala. For comparison, a section of the palatine tonsil was taken from a 49-year-old man who committed suicide by hanging, who had no chronic tonsillitis. Forensic diagnosis: mechanical asphyxia by hanging. Macroscopically: the tonsils are not enlarged, folded, the size of one of them together with the soft tissues was 2.5×1.5×1 cm. Nevertheless, a Congo-positive substance was found in the sections of the suicider's tonsils. It is concluded that a significant number of amyloidosis cases that did not lead to severe damage to the vital organs remain unaccounted for due to the lack of specialists' awareness and, as a result, recommendations for screening for the presence of amyloidosis. At least 30% of patients with chronic tonsillitis are observed to have at least local tonsillar amyloidosis. Amyloid damage to the tonsils can be seen even in clinically unchanged tonsils.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"738 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116084621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER","authors":"Evgeniia A. Grigoreva, V. Gordova, V. Sergeeva, A. Smorodchenko","doi":"10.47026/2413-4864-2021-2-34-43","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-34-43","url":null,"abstract":"The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114568679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EDUCATIONAL OPPORTUNITIES OF \"ONLINE TEST PAD\" IN THE STUDY OF THE DISCIPLINE \"PROPAEDEUTICS OF DENTAL DISEASES»","authors":"L. I. Nikitina, A. Gromova","doi":"10.47026/2413-4864-2021-2-80-85","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-80-85","url":null,"abstract":"Electronic learning tools contribute to the qualitative improvement in future dentists' training. The discipline \"Propaedeutics of dental diseases\" is the primary link in training a professional dental practitioner. The article presents the experience of teaching the discipline \"Propaedeutics of dental diseases\" with the use of electronic and information training technologies. It gives a qualitative assessment to modern teaching methods in the context of digitalization in higher education. The authors' experience in assessing second-year dental students' knowledge and skills in the lessons is described. The conclusion is made about advisability of using electronic tools in teaching, assessing students' knowledge, analyzing academic performance on the example of the \"Online Test Pad\". The main advantages of the test designer \"Online Test Pad\" in the work of a teacher are instant processing of the testing results and objective assessment of the student's knowledge.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121941307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ABOUT THE DELIRIUM OF CORONAVIRUS SELF-CONTEMPT","authors":"A. Golenkov","doi":"10.47026/2413-4864-2021-2-1-6","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-1-6","url":null,"abstract":"Based on observations from forensic psychiatric practice and the review of the literature, we describe for the first time the delirium of the \"coronavirus (self)contempt\". An elderly patient after coming through COVID-19 infection began to consider himself and his wife a distributor of the coronavirus infection, constituting himself a danger to the public. Against the background of feeling of impending doom, he felt the sense of disgrace, shame and self-contempt for himself and expected a negative attitude from others, including discrimination, so he was ready to commit suicide. Under the influence of these delusional ideas, he tried to kill his wife and himself, but by a lucky chance all the defendants in the case remained alive. The forensic psychiatric examination recognized him insane. Delirium of the coronavirus (self)contempt is a sensual delusion that develops against the background of a severe stress (or depression), by the conditions of formation (character, experience, environment) it resembles sensitive reference delusion. The paper discusses the involvement of self-stigmatization (stigma and discrimination) phenomena, the influence of COVID-19 on the development and pathoplasty of delusional (super-valuable) ideas in this psychopathological phenomenon.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115676650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHANGES IN THE INDICATORS OF THE GENERAL BLOOD TEST AND COAGULOGRAM IN A MILD COURSE OF CORONAVIRUS INFECTION","authors":"N. Timofeeva, O. Kostrova, I. S. Stomenskaya, N. V. Bubnova","doi":"10.47026/2413-4864-2021-2-44-49","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-44-49","url":null,"abstract":"In 2020 the pandemic of a new coronavirus infection spread in almost all countries of the world. The danger of this infection lies in the damage to the lungs, which can lead to fatal outcomes. The success of treatment is largely determined by the early diagnosis of the disease and its timely treatment. In order to find the ways of early laboratory diagnosis of coronavirus infection, we studied changes in peripheral blood indicators, coagulograms and C-reactive protein in 56 outpatient patients with mild course of coronavirus infection, whose average age was 49±2 years (among them, 23.2% were men and 66.8% were women). In all patients, coronavirus infection was confirmed by the detection of SARS-CoV-2 RNA by polymerase chain reaction (PCR) in nasopharyngeal or oropharyngeal smears. Signs of lung damage were detected in 66% of patients according to computed tomography findings. The number of red blood cells and the level of hemoglobin in all patients were normal. In 7% of patients, thrombocytopenia was detected, in 5.3% of patients – thrombocytosis. The most pronounced changes are foundin the leucoformula and indicators of the blood coagulation system. In 12.5% of patients without signs of viral pneumonia, leukocytosis was detected. All cases of leukopenia were noted only against the background of lung damage, at this, in 16% lymphopenia was found, 43% had relative lymphocytosis. The coagulogram of more than half of the patients with pneumonia showed shortening of blood clotting in the activated partial thromboplastin time (APTT) test, an increase in prothrombin index(PI) and the content of fibrinogen. The level of C-reactive protein was increased in only a quarter of patients with mild lung damage. Thus, changes in the leucoformula against the background of a tendency to hypercoagulation in blood clotting indicators should cause doctors' apprehensive attitude when treating a patient with clinical symptoms similar to COVID-19.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115567804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY OF THE ANTITUMOR ACTIVITY OF ISOLIQUIRITIGENIN ON THE HELA, PC3, AND MCF-7 TUMOR CELL LINES","authors":"Olga A. Bortnikova, S. Pavlova","doi":"10.47026/2413-4864-2021-2-28-33","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-28-33","url":null,"abstract":"In 2020, the most common type of malignancy was breast cancer. According to WHO, in 2020, this type of cancer accounted for one in eight new cases of cancer in the world and one in four – among women: this disease was diagnosed in 2.3 million people. One of the factors influencing a high mortality rate among patients with this diagnosis can be attributed to an increase in the level of tumor cells resistance to the chemotherapeutic preparations used, so today it is extremely important to search for new preparations that have high specificity for malignant neoplasms, low toxicity and availability for patients. In this regard, a lot of researchers' attention is attracted by compounds of natural origin, in particular the group of flavonoids, which includes isoliquiritigenin. According to the results of modern studies, this compound can have antitumor activity in vitro and in vivo, affecting the molecular mechanisms of regulating the growth and survival of tumor cells. The aim of this article is to study the antitumor antiproliferative activity of isoliquiritigenin on the HeLa, PC-3, and MCF-7 tumor cells lines in vitro. The antiproliferative activity was evaluated using the MTT test with the exposure of cells with the test compound for 72 hours. As a result of the study, isoliquiritigenin showed a dose-dependent antiproliferative effect on human tumor lines PC-3, MCF-7 in the concentration range 10–4–2,5×10–5 mol/l.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122590421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NEUTRALIZATION OF ANGIOPOIETIN-2 AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) FOR THERAPEUTIC PURPOSES","authors":"E. Shamitova, Konstantin G. Matkov, Darya D. Shikhranova, Ramis R. Abdullin","doi":"10.47026/2413-4864-2021-2-64-79","DOIUrl":"https://doi.org/10.47026/2413-4864-2021-2-64-79","url":null,"abstract":"New blood vessels in organs and tissues are formed by angiogenesis, which can take place both in the normal condition and in tumour growth. Angiogenesis supports the strength and integrity of the connections in blood vessel endothelial cells with each other and with the basement membrane. This ensures nutrition of tissues and organs, saturation with oxygen, macronutrients and micronutrients. Along with this, angiogenesis contributes to timely elimination of metabolic products. Metastatic spreading and tumor growth are supported by uncontrolled angiogenesis, that is why it is important to study the works dedicated to neutralization of angiogenesis factors, which becomes a method of combating various oncological diseases and other pathologies. The aim of this work was to study the information about modern drugs, including those at the stage of clinical trials, capable of neutralizing angiopoietin-2 – an inhibitor of angiogenesis and vascular growth factor (VEGF) – an angiogenesis activator, to evaluate the effectiveness and safety of various doses of drugs in various pathologies, to analyze the current state of studying tumor angiogenesis, achievements and prospects for the use of antiangiogenic drugs in oncological practice. The main focus was on the role of angiogenesis inhibitors and activators. To construct and structure the meta-analysis, we conducted a systematic review of the literature, searching works in open Internet resources such as PubMed, CyberLeninka, PsycINFO, Elibrary, published in the period from January 1, 2016 to March 31, 2021, devoted to studies on the effectiveness of drugs aimed at neutralizing angiopoietin-2 and VEGF. Conference materials and dissertations were analyzed to obtain additional data on research in this area.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129194212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COLORIMETRY OF HISTOLOGICAL MATERIAL","authors":"V. A. Kozlov, S. Sapozhnikov","doi":"10.47026/2413-4864-2020-4-7-18","DOIUrl":"https://doi.org/10.47026/2413-4864-2020-4-7-18","url":null,"abstract":"The relevance of the research lies in the use of conventional software products distributed under the GNU license to assess staining of histological material based on the analysis of photomicrographs. The purpose of the study is to demonstrate the opportunities of conventional software distributed under the GNU license, designed for color analysis of photos in histology to identify differences in tinctorial properties of tissues. The authors evaluated the opportunities of the Color Analysis program launched in the Android Nox Player 6.6.1.0 emulator (GNU license) under Windows 10 for color analysis of photomicrographs of various tissues histological sections. Photomicrographs of paraffin sections of 5 microns thick were obtained using a micro-microscope Mikmed-5 using a Levenhuk C800 NG 8M video eyepiece, USB 2.0. A white light lamp Feron G4 4000 K was used as an illuminator. In color analysis of photomicrographs stained with hematoxylin and eosin, or hematoxylin and Congo red, or by Unna, or by the designer three-color amyloid staining in the Color Analysis program, color separation patterns were obtained that reflect the relative area (%) of the field of view staining with shades characteristic for this method of staining. The authors formulate the standard conditions for the preparation of histological specimen and conditions for photographing micro-preparations to obtain comparable results in an experiment with the control-experience design. It is concluded that the program color analysis provides a significant amount of additional information about the histological material, which can be formalized and studied as statistical values.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121910982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELECTROCONVULSIVE THERAPY FOR MENTAL DISORDERS DURING PREGNANCY AND THE POSTPARTUM PERIOD","authors":"F. Orlov","doi":"10.47026/2413-4864-2020-4-44-52","DOIUrl":"https://doi.org/10.47026/2413-4864-2020-4-44-52","url":null,"abstract":"Electroconvulsive therapy is a method of treating mental disorders that is still used today. It is thanks to electroconvulsive therapy that high rates of remission are achieved in patients. The main clinical indications for electroconvulsive therapy are severe depression with a high risk of suicide, life-threatening somatic conditions due to depression. Electroconvulsive therapy is usually used in the first and second trimesters of pregnancy as a last-line method of choice if there are convincing indications for its administration, to quickly eliminate the symptoms. When performing electroconvulsive therapy, the risks of using anesthesia, side effects, severity of the somatic condition, as well as the risks of not using this therapy are evaluated. Although electroconvulsive therapy is considered to be a safe and effective method for treating mental patients if necessary measures are taken to reduce potential risks, it is extremely rarely used to treat women with mental disorders during pregnancy and the postpartum period.","PeriodicalId":249759,"journal":{"name":"Acta medica Eurasica","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127898012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}