COLORIMETRY OF HISTOLOGICAL MATERIAL

Abstract

The relevance of the research lies in the use of conventional software products distributed under the GNU license to assess staining of histological material based on the analysis of photomicrographs. The purpose of the study is to demonstrate the opportunities of conventional software distributed under the GNU license, designed for color analysis of photos in histology to identify differences in tinctorial properties of tissues. The authors evaluated the opportunities of the Color Analysis program launched in the Android Nox Player 6.6.1.0 emulator (GNU license) under Windows 10 for color analysis of photomicrographs of various tissues histological sections. Photomicrographs of paraffin sections of 5 microns thick were obtained using a micro-microscope Mikmed-5 using a Levenhuk C800 NG 8M video eyepiece, USB 2.0. A white light lamp Feron G4 4000 K was used as an illuminator. In color analysis of photomicrographs stained with hematoxylin and eosin, or hematoxylin and Congo red, or by Unna, or by the designer three-color amyloid staining in the Color Analysis program, color separation patterns were obtained that reflect the relative area (%) of the field of view staining with shades characteristic for this method of staining. The authors formulate the standard conditions for the preparation of histological specimen and conditions for photographing micro-preparations to obtain comparable results in an experiment with the control-experience design. It is concluded that the program color analysis provides a significant amount of additional information about the histological material, which can be formalized and studied as statistical values.