cd68阳性大鼠肝脏和脾脏细胞对饮水摄入硅的反应

Evgeniia A. Grigoreva, V. Gordova, V. Sergeeva, A. Smorodchenko
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引用次数: 2

摘要

本文介绍了一种硅化合物与饮用水一起供应(9个月)对实验大鼠肝脏和脾脏cd68阳性巨噬细胞的长期影响的数据——非水合偏硅酸钠(硅含量为10毫克/升)。发现该细胞群的形态学特征发生了变化。平均细胞面积减少(对照组大鼠肝脏平均巨噬细胞面积为179.23±5.94 microns2,饮水硅片组为- 117.04±3.35 microns2;脾脏为136.02±3.93 μ s, 103.44±2.8 μ s。实验组大鼠肝制剂中巨噬细胞突起较少,胞质膜颜色较深。单位面积肝内巨噬细胞数量比较,对照组为18.78±1.24个,加硅水组为19.41±0.75个。实验大鼠接受硅处理后,脾红髓CD68+巨噬细胞的形态也发生了以下变化:它们的位置更密集,突起更少,而每单位面积的巨噬细胞数量,对照组为73.7±2.3个,实验组为91.6±5.0个。他们之间的距离没有改变。肝、脾巨噬细胞胞质膜表面和胞质中CD68表达强度发生变化。这些变化可以解释为肝脏和脾脏巨噬细胞对饮用水中引入硅的适应能力。鉴于肝脏和脾脏巨噬细胞群体的异质性,需要进一步研究巨噬细胞不同亚群的标记物,以阐明它们在组织对饮用水中硅的反应中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER
The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.
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