2011 Functional Optical Imaging最新文献

{"title":"Life sciences require the third dimension with high spatial and temporal resolution","authors":"A. Diaspro","doi":"10.1109/FOI.2011.6154812","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154812","url":null,"abstract":"A recognized advantage of optical microscopy lies in the fact that allows non-invasive three-dimensional (3D) imaging of live cells at the submicron scale with high specificity [1]. The advent of the visible fluorescent proteins [2] and of a myriad of fluorescent tags pushed fluorescence microscopy to become the most popular imaging tool in cell biology. The confocal and multiphoton versions of fluorescence microscopy reinforce this condition. In general, is a well-known paradigm the given inability of a lens-based optical microscope to discern details that are closer together than half of the wavelength of light. Recently, the viewpoint for improving resolution moved from optical solutions to the side of the fluorescent molecule to be detected. Today, for the most popular imaging mode in optical microscopy, i.e. fluorescence, the diffraction barrier is crumbling and the term “optical nanoscopy”, coined earlier, comes to be a real far field optical microscope available for the scientific community as the ones allowing individual molecule localization at high precision [3, 4]. Here we discuss about architectures, calibrations and applications of targeted and stochastic readout methods using both single and multiphoton excitation with emphasis towards three-dimensional imaging with high spatial and temporal resolution [5–7].","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116691559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysing surface plasmon microscopy with rigorous diffraction theory","authors":"Suejit Pechprasarn, M. Somekh","doi":"10.1109/FOI.2011.6154841","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154841","url":null,"abstract":"Surface plasmon (SP) microscopy offers great potential for bioimaging applications particularly for quantitative measurement of refractive index over localized regions. Our aim is to measure variations in refractive index so that important events such as antibody-antigen binding can be measured on smaller spatial scales than is possible with conventional prism based SP imaging. To this end we have used rigorous coupled wave analysis (RCWA) to develop a theoretical framework to understand the capability of the SP microscope to measure local changes in refractive index. Our results show that the non-interferometric microscope configuration gives results which are strongly dependent on the propagation length of the SPs, whereas in the interference mode the measured path of the SPs is determined by the sample defocus and SPs following different paths are not detected. This gives better localization of the measurement of local refractive index as well as a more predictable value as it depends on the optical set rather than sample properties.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"226 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121961620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Superresolution optical endoscopy imaging","authors":"M. Gu","doi":"10.1109/FOI.2011.6154826","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154826","url":null,"abstract":"The integration of femtosecond laser beams and optical microscopy has led to the invention of nonlinear optical microscopy which provides a revolutionary tool for three-dimensional (3D) imaging through thick tissue media. The recent development of fibre optics including photonic crystal fibre and its application into this new microscopy method has facilitated the emerging of nonlinear optical endoscopy for in vivo diagnosis and therapeutic studies. In this paper we present our recent cutting-edge progress on functional handheld fibre-optical nonlinear endoscopy and its application as a minimal invasive method for simultaneous diagnoses and treatment of cancer cells using transferrin-conjugated gold nanorods that are specifically labelled to cancer cells. In particular, we will report on a novel nonlinear optical endoscope using laser beams at two wavelengths for 3D superresolution imaging.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127888834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization and dynamics of the serotonin1A receptor in live cells using fluorescence microscopy","authors":"A. Chattopadhyay","doi":"10.1109/FOI.2011.6154813","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154813","url":null,"abstract":"It is important to understand the dynamic organization of membrane-bound molecules in order to arrive at a comprehensive view of cellular signaling mediated by membrane-bound receptors.1 We addressed the organization and dynamics of the human serotonin1A receptor fused to enhanced yellow fluorescent protein expressed in CHO cells. Serotonin1A receptors are prototypical members of the G-protein coupled receptor superfamily and represent a prime target for therapeutic actions of several anxiolytic and antidepressant drugs.2 Interestingly, we observed significant retention in fluorescence of serotonin1A receptors upon Triton X-100 treatment of intact cells at low temperature demonstrating their detergent insolubility.3 We analyzed the role of cholesterol in the plasma membrane organization of the serotonin1A receptor by fluorescence recovery after photobleaching (FRAP) measurements with varying bleach spot sizes. Our results show that lateral diffusion parameters of serotonin1A receptors are altered in cholesterol-depleted cells in a manner that is consistent with dynamic confinement of serotonin1A receptors in the plasma membrane.4 Our recent work using z-scan fluorescence correlation spectroscopy (zFCS) provides novel insight on the effects of cholesterol depletion and actin cytoskeleton destabilization on receptor confinement.5 Interestingly, results from FRAP measurements performed under conditions of mild cytoskeletal destabilization suggest that receptor signaling is correlated with receptor mobility, in agreement with the ‘mobile receptor hypothesis’.6 In addition, we developed a novel microscopy-based image reconstruction approach to quantitatively monitor dynamic changes in actin cytoskeletal network upon signaling.7 We recently proposed utilizing Homo-FRET in live cells, that the serotonin1A receptor is present as constitutive oligomers and implicated the presence of higher-order oligomers.8 Taken together, these results on the cellular organization and dynamics of the serotonin1A receptor would be valuable in understanding the function of the receptor in healthy and diseased states.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134439212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-contrast imaging of moving targets","authors":"Bo Fu, Noah A. Russell","doi":"10.1109/FOI.2011.6154847","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154847","url":null,"abstract":"The imaging of cells flowing through blood vessels can be a useful diagnostic tool'. However, live samples are usually transparent and contrast is poor with bright-field microscopy. A variety of methods are available to improve contrast including; phase contrast, differential interference contrast, fluorescent labelling and surface plasmon resonance imaging. These methods are not all amenable to automated cell detection and analysis, however, because the cell is not easily separable from the background.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114320370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growing neural networks on gold surface plasmon resonance imaging sensors","authors":"D. Albutt, M. Alexander, Noah A. Russell","doi":"10.1109/FOI.2011.6154846","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154846","url":null,"abstract":"Surface plasmon resonance (SPR) is sensitive to changes of refractive index at a metal-dielectric interface. This technique has been applied to image networks of neurons non-invasively. The long term survival of active neural networks on SPR sensors requires optimisation of both the cell culture and the surface chemistry to ensure neurons adhere and grow uniformly.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122624139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New approaches for high speed, high resolution in vitro cardiac imaging","authors":"G. Bub","doi":"10.1109/FOI.2011.6154817","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154817","url":null,"abstract":"Cameras used in functional cardial imaging studies are limited to low spatial resolutions in order to reach the frame rates required for mapping fast activation patterns in the intact heart. As a result, single pixels integrate signal from a large tissue volume, which leads to order of maginitude underestimates of action potential upstroke velocities. Temporal pixel multiplexing (TPM) is a new imaging modality that enables high resolution sensors to be used for high speed imaging tasks. A TPM imaging system was used to map voltage transients in a di-4-ANEPPS loaded Langendorff perfused guinea pig heart preparation at kilohertz rates. The TPM system measured action potential upstroke velocities that are far faster than those mapped with conventional sensors; captured values closely match theoretical limits determined from photon scattering simulations of voltage sensitive dye loaded heart preparations. TPM holds promise for overcoming limitations associated with conventional imaging modalities.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"345 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122839550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro range imaging based on structured illumination","authors":"Xiang Peng, A-meng Li, Yongkai Yin, Xiaoli Liu, Klaus Korner, G. Pedrini, W. Osten","doi":"10.1109/FOI.2011.6154835","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154835","url":null,"abstract":"Quantitative characterization and imaging of three-dimensional (3D) microstructures using optical methods are of great concerns in both scientific research and practical applications, such as life science and micro systems technology (MST). A number of optical principles have been applied for different types of 3D microscopy. Laser scanning confocal microscope (LASCM), interferometric microscope (IM), Zernike phase contrast (ZPC), differential interference contrast (DIC), optical coherence tomography (OCT) and digital holographic microscope (DHM) are typical representatives for 3D micro imaging. Most of mentioned technique for 3D microscopy have been already commercialized and are currently available on the market. However, not all of those optical imaging methods are suitable for quantitative assessment of microstructures but only for qualitative observations with a few exceptions like DHM and IM. Quantitative analysis and characterization of 3D microstructures have become increasingly important as the development of microsystems, including micro-optics, micro fluidics and lab-on-chips. Structured illumination based range imaging on (SIRI) such as fringe projection profilometry has been widely used in industry and scientific research. Typical examples of range imaging in a variety of application fields include art, architecture, archeology, medical imaging, industrial inspection, reverse engineering, virtual reality, to name just a few. The SIRI works well in a moderate scale in terms of target size. In contrast with the SIRI in macro scale, the research of micro range imaging based on structured illumination (μSIRI) receives much less attention. However, the μSIRI could be a promising candidate for quantitative three-dimensional (3D) imaging in microscopy due to its unique features such as fast acquisition, high data density, and cost-effective configuration. This talk will address some important issues regarding the μSIRI as an enabling tool for quantitative 3D imaging in microscopy. The main issues to be discussed involve in reformulating the image formation of the μSIRI by introducing a concept of active micro stereoscopy, characterizing the lateral and longitudinal resolutions of the μSIRI microscopy in three dimensions and suggesting a strategy of calibration for the μSIRI microscope. Some preliminary experiment results are also presented to verify the presented approach.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124408798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}