2011 Functional Optical Imaging最新文献_第3页

Confocal surface plasmon resonance microscopy with pupil function engineering 共聚焦表面等离子体共振显微镜与瞳孔功能工程

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154815

Bei Zhang, Suejit Pechprasarn, M. Somekh

引用次数: 0

Optical Tweezers Based Bio-Microrheology 基于光学镊子的生物微流变学

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154828

A. Chiou

{"title":"Optical Tweezers Based Bio-Microrheology","authors":"A. Chiou","doi":"10.1109/FOI.2011.6154828","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154828","url":null,"abstract":"Viscoelastic properties of a myriad of biological cells and bio-fluids are strongly correlated to their physiological functions; a change in their viscoelastic properties, even at a very small fraction on the order of a few percents, is often concomitant with related diseases. A classical example is the red blood cells (RBCs) whose deformability is critical to their oxgen-carrying and delivery function through veins and arteries. In the case of biological fluids, the viscosity of blood is closely related to cerebral vascular disease and coronary artery disease, and the liquefaction of vitreous humor can lead to retinal detachment. The relation of viscoelastic alterations of cerebral-spinal fluid and hydrocephalus has also been reported. Accurate measurement of the viscoelastic properties of biological samples at either single cell resolution (for the case of biological cells) or with sample volume on the order of micro-liter (for the case of biological fluids) may hence shed light on clinically-relevant mechano-biology at the molecular lever. From the practical point of view, the requirement of only a very small amount of sample is particularly important since many biological fluids such as synovial fluid and vitreous humor are available only in limited amount. Optical-Tweezers Based Micro-Rheology has emerged in recent years as one of the critical techniques capable of fulfilling the needs elucidated above.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126490737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a robust wide field surface plasmon instrument as part of a platform for Alzheimer's disease diagnosis 开发一个强大的宽场表面等离子体仪器作为阿尔茨海默病诊断平台的一部分

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154822

Richard J. Smith, R. Light, Nicholas S. Johnston, Jing Wang, P. O'shea, M. Somekh

引用次数: 0

Cell image based on reassembly of the host-guest complex of CB[8] 基于CB主客体复合体重组的细胞图像[8]

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154839

Shiguo Sun, Fusheng Li

{"title":"Cell image based on reassembly of the host-guest complex of CB[8]","authors":"Shiguo Sun, Fusheng Li","doi":"10.1109/FOI.2011.6154839","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154839","url":null,"abstract":"Being a relatively new class of host molecule, cucurbit[8]uril (CB[8], Scheme 1) has been studied extensively and used in many areas such as molecular machine, molecular switches and molecular wires etc[1–3]. While, acridine orange (AO)[4] is capable of cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions respectively, so it is widely used for cell staining, DNA intercalator, cell cycle determination and transmembrane pH-gradient probe etc. Reassembly of the host-guest complex between AO and CB[8] with DNA/RNA has been studied and employed to fluorescent detect DNA/RNA. The dye is observed to leave the cavity of CB[8] and reassemble (bind) with DNA/RNA immediately, accompanying a strong fluorescence increasing effect, the fluorescence intensities are linearly proportional to the amount of DNA/RNA added, the sensitivity can be improved because the background signals of AO can be significantly decreased due to the dimeric dye formed in CB[8] cavity.[5] When AO is replaced by another dye pyronine Y (PYY), no efficient response can be observed on RNA, which can be used to distinguish DNA from RNA. Not much difference can be observed on fluorescence ratio image of Hela cells for the tricyclic basic dyes before and after including of CB[8], demonstrating a potential application of this method, which can be employed as a convenient and efficient way, to solve autofluorescence problem of the tricyclic basic dyes instead of normally complicated organic synthesis.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125116419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence ratiometry and fluorescence lifetime (FLIM) imaging: Dual mode imaging cellular viscosity by a single molecular rotor 荧光比率法和荧光寿命(FLIM)成像:单分子转子双模式成像细胞粘度

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154843

Zhigang Yang, Jiangli Fan, Xiaojun Peng

引用次数: 2

Coming opportunities for nonlinear optical microscopy - with focus on molecular dynamics imaging 非线性光学显微镜的未来机遇-聚焦于分子动力学成像

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154816

F. Kao

引用次数: 0

Silicon on Insulator photonic wire based evanescent field multi-channelled biosensor array “Lab on a Chip” devices 基于硅绝缘体光子线的倏逝场多通道生物传感器阵列“片上实验室”装置

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154834

Yaping Zhang

{"title":"Silicon on Insulator photonic wire based evanescent field multi-channelled biosensor array “Lab on a Chip” devices","authors":"Yaping Zhang","doi":"10.1109/FOI.2011.6154834","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154834","url":null,"abstract":"A series of Silicon on Insulator (SOI) photonic wire based evanescent field multi-channelled biosensor array “Lab on a Chip” device configurations and designs are presented, which are based on the author's biosensor patent granted in the UK. This work employs the most advanced chip design, fabrication and characterisation technologies developed from the telecom applications. The chip design capitalises on the most sensitive detection method, i.e. the evanescence field and interferometry technology, and implements the multi-mode interferometer (MMI) technology to integrate the multi-channelled biosensor arrays onto a single chip. This design enables the label-free, time domain real time parallel detection and identification of various analytes and molecular interactions in one or more test samples at extremely low concentrations, with extraordinarily high accuracy and sensitivity of detection simultaneously and instantaneously. For example, the limit of detection of low concentrations of molecules at or even below 1×10−12 M can be achieved. This corresponds to an effective refractive index resolution at a magnitude of 10−8 or better. The devices are robust, all-optical, highly scalable, energy efficient dynamic sensing devices. Individual sensor can be addressed separately if wish, and with built-in internal reference channels integrated on chip. Sensing and reference channels can be grouped in an array to allow maximum flexibility in measuring individual analytes in a sample or a number of samples simultaneously and instantaneously. This research and development work could potentially open the door to the next generation of low-cost mass production evanescent field multi-channelled biosensor array “Lab on a Chip” instrumentation development for various application fields, and provide a novel way forward towards revolutionising modern analytical technology.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"292 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116095006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optical trapping with cylindrical vector beams 圆柱形矢量光束的光捕获

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154814

Baoli Yao, Shaohui Yan, M. Lei, Fei Peng, Baiheng Ma, Tong Ye

{"title":"Optical trapping with cylindrical vector beams","authors":"Baoli Yao, Shaohui Yan, M. Lei, Fei Peng, Baiheng Ma, Tong Ye","doi":"10.1109/FOI.2011.6154814","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154814","url":null,"abstract":"Recent development of cylindrical vector beams prompts its application in optical trapping, which shows more effective and improved trapping efficiency in contrast to the traditional Gaussian beam of spatially homogeneous polarization. Using the T-matrix method and vectorial diffraction theory, we calculated and compared the radiation forces exerted on dielectric particles respectively by the linearly polarized, radially polarized and azimuthally polarized beams. Theoretical calculations show that the radially polarized beam can improve the axial trapping efficiency of high-refractive-index larger particles by reducing the scattering force due to the vanishing axial component of Poynting vector near the focus, while the azimuthally polarized beam can not only steadily trap low-refractive-index small particles at the focus center but also can trap multiple high-refractive-index particles around the focus center in virtue of the hollow-ring configuration. The dependences of the trapping efficiencies on the beam parameters, particle size and the numerical aperture of objective lens are discussed. The performances of optical trapping, manipulating and sorting of biological cells, organelles and various micro-particles are demonstrated.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"747 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116098851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Long-term imaging of fibroblasts in a microfluidic device 微流控装置中成纤维细胞的长期成像

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154845

Noah A. Russell, Alex D. Johnstone, M. Alexander

引用次数: 1

Plasmonic-array-based biosensors for the diagnosis of neurodegenerative diseases 用于神经退行性疾病诊断的等离子体阵列生物传感器

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154818

K. Vere, J. Richens, M. Somekh, N. Bajaj, K. Morgan, P. O'shea

{"title":"Plasmonic-array-based biosensors for the diagnosis of neurodegenerative diseases","authors":"K. Vere, J. Richens, M. Somekh, N. Bajaj, K. Morgan, P. O'shea","doi":"10.1109/FOI.2011.6154818","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154818","url":null,"abstract":"The impact of Alzheimer's disease (AD) is projected to become one of the major challenges to modern society: over the next few years the incidence of AD will reach 1 million people in the UK alone with an estimated annual economic cost of £30 billion. Unfortunately, the existing diagnostic procedures are inadequate for early disease detection and do not always differentiate AD from other dementias. In addition, they are time consuming, expensive and of limited availability outside specialist centres. Definitive AD diagnosis is still only available post-mortem and the development of enhanced diagnostic strategies are, therefore, highly desirable. We have identified a panel of AD biomarkers incorporating proteins whose diagnostic power arises from their interrelated patterns and we are developing an instrument for point-of-care diagnostic tests that can be used in clinical settings (community medical centres and hospitals) as a screening tool for these markers. Availability of a reliable biomarker test for AD would increase the accuracy of disease diagnosis, aid earlier disease detection including automation for community screening and allow for improved treatment targeting. Whilst no cure for AD is currently available, earlier, more accurate and cost-effective detection of AD will be an important component for facilitating development and implementation of newer, disease modifying therapies (amyloid vaccines). Our approach would also allow expensive resources, particularly emerging state-of-the-art imaging technologies, to be more effectively utilised","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122766221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2