2011 Functional Optical Imaging最新文献

Plasmonic manipulation through light control and its applications in microscopic imaging and sensing 光控等离子体操纵及其在显微成像和传感中的应用

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154829

X. Yuan

引用次数: 0

Sensor based on angular interrogation of Surface Plasmon Resonance by using an elliptical reflector structure 基于椭圆反射结构的表面等离子体共振角探测传感器

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154824

Laixu Gao, Yan Huang, Songquan Li, Hong-an Ye

{"title":"Sensor based on angular interrogation of Surface Plasmon Resonance by using an elliptical reflector structure","authors":"Laixu Gao, Yan Huang, Songquan Li, Hong-an Ye","doi":"10.1109/FOI.2011.6154824","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154824","url":null,"abstract":"We report a method to make simplification and improve stability of Surface Plasmon Resonance (SPR) sensor by using an elliptical reflector in angular interrogation. The sensitive plate is K7 glass coated with about 50nm gold film. The centers of sensitive plate and the detector are set on each of the two focus of the elliptical reflector respectively. According to the geometrical optics, the detector on one focus can receive the light reflected by the elliptical reflector from another focus when incident angle of input light changes continuously. In addition, we use an opto-coupler switch to adjust the starting point of the rotary stage. The angular interrogation method has been used to study the performance of the sensor in terms of intrinsic sensitivity that includes the width and shifts of the SPR curve for a given refractive index of sensing layer. SPR curves can be observed through the rotation of angular movement of stepper motor, then the resonance angle of gold film is calculated. The preliminary experimental results confirmed that structure can be used as a steady sensor that could detect refractive index changes by precise measurement of the resonance angle and develop this sensor as a practicable high-stability biosensing device.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126158749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Micro-Optical Sectioning Tomography for visualizing the mouse brainwide neuroanatomical connectivity 用于观察小鼠全脑神经解剖连通性的显微光学断层扫描

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154830

Qingming Luo

引用次数: 0

Real-time auto-focus implementation 实时自动对焦实现

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154844

S. Idinyang, Noah A. Russell

引用次数: 2

Self-administering neuronal networks 自我管理神经网络

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154842

Paul E. Goodwin, Noah A. Russell

引用次数: 0

Non-invasive, label free, quantitative characterisation of live cells in monolayer culture 无创，无标记，单层培养活细胞的定量表征

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154837

Jing Zhang, D. Morris, V. Sottile, J. Crowe, M. Somekh, M. Mather

引用次数: 0

Label-free biosensing arrays based on phase-sensitive surface plasmon resonance imaging 基于相敏表面等离子体共振成像的无标记生物传感阵列

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154827

Y. Huang, H. Kwok, S.Y. Wu, Y. Shao, H. Ho, S. Kong

引用次数: 0

Optical recording of fast neuron activities 快速神经元活动的光学记录

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154820

S. J. Kim, Jonghwan Lee

{"title":"Optical recording of fast neuron activities","authors":"S. J. Kim, Jonghwan Lee","doi":"10.1109/FOI.2011.6154820","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154820","url":null,"abstract":"Spatiotemporal imaging of neuronal activity in the brain plays a critical role in studies of brain function and disorders. However, thorough monitoring of neuronal activity in the brain has been far from practical due to the limitations of conventional technologies. Since brain activity is fundamentally the ensemble of excitation of neuronal cells, it exhibits extremely high spatiotemporal heterogeneity on the micrometer and millisecond scale. Further, a number of functional studies are accompanied by behavioral control and/or observation. An ideal functional imaging technology, therefore, would involve noninvasive, label-free and three-dimensional dynamic imaging of cortical neuronal activity with µm and ms resolution. Optical methods look like one of the most feasible approaches because fast optical signals of neuronal activity have several advantages over conventional methods, including the advantages that they require no probe and thus can be recorded noninvasively in 3D. It is logical to start from validating the measurement of fast optical signals in the fundamental unit of the neural system (neurons) and then pursue toward the measurement in the most complex neural system (human brain). As several scientists had reported the measurement of fast optical signals in large neurons isolated from low-level animals, we performed the next step in this study - optical measurement of neuronal activity in ex vivo brain tissue.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130274356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Framing next-generation imaging questions: Outstanding problems that must be addressed if we are to understand cell biology 构建下一代成像问题:如果我们要理解细胞生物学，必须解决的突出问题

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154823

P. O'shea, J. Richens, K. Vere

{"title":"Framing next-generation imaging questions: Outstanding problems that must be addressed if we are to understand cell biology","authors":"P. O'shea, J. Richens, K. Vere","doi":"10.1109/FOI.2011.6154823","DOIUrl":"https://doi.org/10.1109/FOI.2011.6154823","url":null,"abstract":"It is clear that the enormous variety of imaging technologies particularly those directed towards single cells have transformed our understanding of how living cells function. It is also evident that this field of study has matured to the extent that bioimaging is itself almost a self-standing discipline. There remains, however, quite profound problems both in terms of the imaging technologies themselves and also those that are presented to us by cell biology. We must become more aware that imaging protocols may modify the processes we are observing, there is growing body of evidence for example, that large photon dosages associated with eg stimulated emission depletion imaging or PALM/STORM imaging to a region of a single living cells can lead to cellular damage or modification of molecular behavior in the vicinity of the laser irradiation. This potential problem is related to both high intensity and long exposure times. Similarly, some techniques that operate towards the edge of present detection sensitivities (eg single molecule techniques) requiring extended illumination times necessary to acquire an image are also subject to some concerns that the measurement is modifying the process under scrutiny. Another problem that is becoming the subject of greater awareness is that the attachment of a photoprotein to a protein of interest (PoI) may lead to modified behaviour of the PoI or even other protein that are affected by the augmented exposure of the photoprotein-PoI construct. It should be emphasised, however, that these foregoing comments in no way represent any criticism of the profound contribution the originators of these techniques have made, they are made simply to allow us to formulate a ‘wish-list’ for our next generation imaging needs.","PeriodicalId":240419,"journal":{"name":"2011 Functional Optical Imaging","volume":"120 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129377551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical system for selectively firing neurons 选择性激发神经元的光学系统

2011 Functional Optical Imaging Pub Date : 2011-12-01 DOI: 10.1109/FOI.2011.6154849

Alex D. Johnstone, Bo Fu, Noah A. Russell

引用次数: 1