中华预防医学杂志最新文献

{"title":"[Molecular epidemiological characteristics and risk factors analysis of Carbapenem-resistant Enterobacterales intestines colonization of neonates in Shenzhen region].","authors":"H M Yang, K Cao, Z L Xiong, X C Liu, K Y Yang, Y X He, S X Lin, J H Zou, S Y Liu, T Y Ding, L F Yin, Z X Li, C H Duan, Z W Zhou","doi":"10.3760/cma.j.cn112150-20250207-00091","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250207-00091","url":null,"abstract":"<p><p><b>Objective:</b> To study the risk factors and the molecular epidemiology characteristics for Carbapenem-resistant Enterobacteriaceae(CRE) colonization in neonatal inpatients in Shenzhen region, China, which provide reference for the prevention and control of clinical CRE infection. <b>Methods:</b> This study is a prospective case-control study.Anal samples from inpatients between January 2023 and December 2023 at Longgang Maternity and Child Institute of Shantou University Medical College and Shenzhen Children's Hospital were collected for screening CRE strain. Drug susceptibility test, modified Carbapenem Inactivation Method (mCIM) test, drug resistance-related gene sequencing and multilocus sequence typing (MLST) were performed for isolated CRE strains.Meanwhile, the clinical data were collected for analyzing the risk factors of CRE intestinal colonization by multivariate regression analysis. <b>Results:</b> A total of 1 517 patients were screened, 26 CRE(1.7%, 26/1 517) were identified which including 14 Escherichia coli(53.8%, 14/26), 11 Klebsiella pneumoniae(42.3%, 11/26), 1 Enterobacter cloacae(3.9%, 1/26). The predominant carbapenemase gene was New Delhi Metallo(NDM) (92.4%, 24/26), followed by Imipenem (IMP) (3.8%, 1/26) and Guiana extended spectrum gene (GES) (3.8%, 1/26).Among the carried NDM resistance genes, New Delhi Metallo 5 (NDM5) was the main one, accounting for 84.6% (22/26).The MLST typing of Escherichia coli was mainly Sequence Type 48 (ST48) (6/14), while that of Klebsiella pneumoniae was mainly Sequence Type 35 (ST35) (10/11). All CRE isolates were resistant to penicillin, penicillinase inhibitors, cephalosporins, ertapenem and imipenem.The resistance rates of Escherichia coli to amikacin, levofloxacin was 1/14, 4/14, respectively. All isolates of Klebsiella pneumoniae were sensitive to amikacin, and the resistance rate to levofloxacin is 1/11. Risk factors for CRE colonization include the older age, length of hospital stay, tracheal intubation, invasive respiration, lumbar puncture, Apgar <7 score, and exposure to antibiotics. <b>Conclusions:</b> NDM5 is the predominant resistant gene in CRE isolated from neonatal patients feces in Shenzhen region.It is necessary to strengthen the screening of CRE colonization in neonate for prevention and control of CRE infection.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1022-1030"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathogenetic characteristics of human isolates of <i>Streptococcus suis</i> in Henan Province from 2020 to 2023].","authors":"X Luo, B F Zhang, Y J Mu, Y F Li, H X Ma, H F Wang, Y Ye, X Y Huang, W S Guo","doi":"10.3760/cma.j.cn112150-20240929-00783","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240929-00783","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the pathogenetic characteristics of clinical isolates of <i>Streptococcus suis</i> in Henan Province from 2020 to 2023. <b>Methods:</b> Eight clinical isolates of <i>S. suis</i> in Henan Province from 2020 to 2023 were identified using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and real-time fluorescence polymerase chain reaction (PCR). Serotype and virulence genes were detected by the serum agglutination test and PCR, and antibiotic susceptibility was evaluated using the microbroth dilution method. Multilocus sequence typing (MLST), minimum core genome (MCG), identification of antibiotic resistance genes, and core genome single nucleotide polymorphism (cgSNP) analysis were conducted using whole genome sequencing. <b>Results:</b> The results showed that eight <i>S. suis</i> strains isolated from humans were mainly serotype 2 (75.0%), while the rest were serotype 14 (25.0%). ST353 (62.5%) was the predominant genotype, followed by ST1 (25.0%) and ST7 (12.5%). All isolates belonged to the MCG1 group. The virulence genotypes of these isolates were primarily <i>mrp</i>(NA2)/<i>sly</i>+/<i>ef</i>+/<i>gapdh</i>+(75.0%), while the remaining were <i>mrp</i>(EU)/<i>sly</i>+/<i>ef</i>+/<i>gapdh</i>+(25.0%). These isolates carried tetracycline, macrolide, lincosamide and aminoglycoside resistance genes, and their resistance rates to tetracycline, erythromycin and clindamycin were 100.0%, 87.5% and 87.5%, respectively, and 62.5% strains were intermediate-resistant to penicillin. The cgSNP analysis indicated that these isolates were closer to the isolates from Guangdong, Zhejiang and Guangxi Provinces, with five ST353 strains and one ST7 strain belonging to Clade Ⅰ, and two ST1 strains belonging to Clade Ⅱ. <b>Conclusion:</b> The human isolates of <i>S. suis</i> in Henan Province are mainly ST353, harboring multiple virulence and antibiotic resistance genes.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"989-996"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Bioinformatics analysis and purification of <i>Treponema pallidum</i> OmpH protein and preparation of polyclonal antibody].","authors":"X Wu, J Jiang, X F Wang, M Wang, H Yang, S G He, Y D Cao","doi":"10.3760/cma.j.cn112150-20250319-00216","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250319-00216","url":null,"abstract":"<p><p><b>Objective:</b> To analyze and predict the biological properties and function of <i>Treponema pallidum</i> OmpH protein by bioinformatics methods, purify the target protein, and prepare polyclonal antibodies. <b>Methods:</b> From January 2024 to February 2025, the research team from the Department of Clinical Laboratory at The First Affiliated Hospital of Hunan Traditional Chinese Medical College (Hunan Province Directly Affiliated Traditional Chinese Medical Hospital) conducted a study employing integrative approaches combining bioinformatics analysis with animal experimentation. During this investigation, the coding sequence of the <i>T. pallidum</i> outer membrane protein H (TpOmpH) was systematically retrieved from the National Center for Biotechnology Information (NCBI) database. And the bioinformatics tools, such as Protparam, Protscale,SignalP 6.0,NetNGlyc-1.0,TMHMM2.0,NetPhos-3.1,SOPMA,AlphaFold3,IEDB,STRING,C-immsim were used to analyze and predict the biological and immunological characteristics of TpOmpH protein. The full length of TpOmpH gene was synthesized and was cloned into the pET28a to construct the recombinant plasmid pET28a-TpOmpH. The the expression of target protein was induced by IPTG and was purified using affinity chromatography. The TpOmpH protein was used to immunize mice and the anti-serum was harvested, then the titer of antibody was detected. <b>Results:</b> TpOmpH is a hydrophobic outer membrane protein with a molecular weight of 19.7 kDa and strong stability. The TpOmpH protein is located outside the cell membrane and contains 11 serine, 4 threonine, and 1 tyrosine phosphorylation site, but no glycosylation sites. The 77.91% of the amino acids in TpOmpH protein are alpha helix, 8.72% are extended strand, 10.47% are random coils, and 2.91% are beta turns. The tertiary structure predicted by AlphaFold3 is in its optimal state. The TpOmpH protein has 4 CTL epitopes, 4 linear epitopes, and 5 spatial epitopes. The TpOmpH protein can interact with Tp92,MutS,SurA,TPANIC_0600 and other proteins which may be involved in Tp invasion. TpOmpH protein can induce an increase in B cell count, antibody content, Th cell count, NK cell count, as well as the expression of various cytokines. High purity TpOmpH protein was obtained through Ni²⁺ affinity chromatography, which is consistent with the theoretical molecular weight. TpOmpH protein can induce mice to secrete polyclonal antibodies with antibody titers higher than 1∶10 000. <b>Conclusion:</b> TpOmpH protein is a hydrophobic protein located on the outer membrane of Tp, can induce mice to secrete high titer antibodies, which providing experimental basis for the pathogenesis of Tp and vaccine development.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1013-1021"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the association between cardiometabolic risk and emotional disorders in children and adolescents].","authors":"Z Y Ruan, F B Tao, X Y Wu","doi":"10.3760/cma.j.cn112150-20241101-00869","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20241101-00869","url":null,"abstract":"<p><p>The prevalence of cardiovascular diseases among children and adolescents continues to escalate, with cardiometabolic risk factors emerging as primary contributors to this concerning trend. Key contributing factors encompass obesity, diabetes mellitus, and hypertension. This developmental phase represents a crucial window for both physical maturation and psychological growth. Previous studies have demonstrated significant associations between cardiovascular pathologies and mental health outcomes. Given their immature physiological and psychological regulatory systems, children and adolescents exhibit heightened susceptibility to emotional dysregulation, predominantly manifesting as anxiety disorders and depressive conditions.Current research remains limited in elucidating the complex relationship between cardiometabolic risk profiles and affective disorders within this vulnerable population. Emerging evidence suggests that cardiometabolic disturbances may potentiate the pathogenesis and progression of mood disorders through multifaceted mechanisms including oxidative stress pathways, neuroendocrine system dysregulation, and maladaptive lifestyle patterns. However, substantial gaps persist in our understanding of the precise pathophysiological interactions underlying this association.This comprehensive review systematically examines the interconnection between cardiometabolic risk parameters and emotional disorders in pediatric populations. This article critically evaluate current assessment methodologies, epidemiological patterns, and clinical correlations while proposing mechanistic frameworks that integrate biochemical, neurological, and behavioral pathways. The analysis emphasizes the need for interdisciplinary approaches to address this pressing public health challenge.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1118-1124"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of serum cotinine cut-off value for distinguishing smoking status among Chinese adults].","authors":"C M Ding, J Yin, F Zhao, Y W Li, Y Zhu, Y B Lyu, X M Shi","doi":"10.3760/cma.j.cn112150-20240902-00696","DOIUrl":"10.3760/cma.j.cn112150-20240902-00696","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To determine the optimal cut-off value of serum cotinine for distinguishing smoking status among Chinese adults based on a large-scale national sample. &lt;b&gt;Methods:&lt;/b&gt; A cross-sectional study was conducted among 8 987 Chinese adults aged 20-79 years from 152 administrative counties across 31 provinces during 2017-2018. Sociodemographic characteristics, lifestyle, smoking status, and health status were collected via questionnaires and physical examinations. Blood samples were analyzed for serum cotinine levels using liquid chromatography-mass spectrometry and for blood creatinine levels using the picric acid method. Receiver operating characteristic (ROC) curve analysis was performed with serum cotinine concentration as the test variable and self-reported smoking status as the state variable. The optimal cut-off value was determined based on the maximum Youden's index, and the bootstrap method was used for repeated sampling (2 000 times) to evaluate the confidence interval of the cut-off value. The net reclassification index (NRI) was used to evaluate the discrimination ability of the cut-off value of this study, the cut-off value of the American population 1 (total population: 3.3 μg/L, men: 4.1 μg/L, women: 3.0 μg/L) and the cut-off value of the American population 2 (the recommended value of the United States Centers for Disease Control and Prevention for the total population: 10.0 μg/L) against the smoking status of the Chinese population. Statistical analyses were conducted using IBM SPSS 27 and Python 3.11, with a significance level of α=0.05. &lt;b&gt;Results:&lt;/b&gt; The age of the research subjects was (49.2±15.2) years. Among them, males accounted for 49.8% (4 477); smokers accounted for 28.8% (2 586); the detection rate of serum cotinine was 94.6% (8 501), and the &lt;i&gt;M&lt;/i&gt; (&lt;i&gt;Q&lt;/i&gt;&lt;sub&gt;1&lt;/sub&gt;&lt;i&gt;, Q&lt;/i&gt;&lt;sub&gt;3&lt;/sub&gt;) concentration of serum cotinine was 0.9 (0.3, 85.4) μg/L. The ROC curve analysis results showed that the cut-off value (95%&lt;i&gt;CI&lt;/i&gt;) of serum cotinine in the total population was 8.8 (6.7-11.7) μg/L, with the specificity (95%&lt;i&gt;CI&lt;/i&gt;) about 93.6%(92.7%-94.3%), the sensitivity (95%&lt;i&gt;CI&lt;/i&gt;) about 91.0%(89.7%-92.3%) and the area under the curve (AUC) (95%&lt;i&gt;CI&lt;/i&gt;) about 0.93 (0.92-0.94). The cut-off value (95%&lt;i&gt;CI&lt;/i&gt;) of cotinine for males was 17.1 (8.8-21.8) μg/L, with the specificity (95%&lt;i&gt;CI&lt;/i&gt;) about 90.7%(87.9%-92.0%), the sensitivity (95%&lt;i&gt;CI&lt;/i&gt;) about 89.4%(88.4%-92.2%) and the AUC (95%&lt;i&gt;CI&lt;/i&gt;) about 0.92 (0.91-0.93). The cut-off value (95%&lt;i&gt;CI&lt;/i&gt;) of cotinine for females was 7.4 (3.3-15.0) μg/L, with the specificity (95%&lt;i&gt;CI&lt;/i&gt;) about 95.6%(92.7%-96.8%), the sensitivity (95%&lt;i&gt;CI&lt;/i&gt;) about 87.6%(81.6%-92.8%) and the AUC (95%&lt;i&gt;CI&lt;/i&gt;) about 0.92 (0.87-0.95). The NRI analysis results showed that compared with the cut-off value of the American population 2, the NRI of this study's cut-off values in the total population, males and females were 0.020 (&lt;i&gt;P&lt;/i&gt;=0.015), 0.033 (&lt;i&gt;P&lt;/i&gt;=0.015) ","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1063-1068"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on melatonin in preventing and treating postmenopausal osteoporosis].","authors":"J C Wang, Z Y Xu, Y J Xu, Z F Zhang","doi":"10.3760/cma.j.cn112150-20250225-00137","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250225-00137","url":null,"abstract":"<p><p>Postmenopausal osteoporosis (PMOP), a bone metabolic imbalance disorder induced by estrogen deficiency, necessitates novel prevention and treatment strategies that transcend the constraints of conventional therapeutic approaches. Melatonin, a signaling molecule secreted by the pineal gland, plays a key role in maintaining circadian rhythms and regulating bone metabolism. In recent years, numerous studies both domestically and internationally have demonstrated that melatonin can promote the generation of osteoblasts and osteoclasts, thereby increasing bone density and improving symptoms of osteoporosis. This article reviews the role of melatonin in the prevention of PMOP, aiming to provide new ideas for the diagnosis and treatment of PMOP.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1164-1169"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The practice and exploration on the continuous medical service in children's hospital].","authors":"J J Yuan, X L Tang, T T Jing, Z H Zhu, Y F Wu, X W Hu, L Hong, H Zhang","doi":"10.3760/cma.j.cn112150-20250322-00230","DOIUrl":"10.3760/cma.j.cn112150-20250322-00230","url":null,"abstract":"<p><p>To summarize the clinical practice of continuous medical service for patients at Shanghai Children's Medical Center, affiliated with Shanghai Jiao Tong University School of Medicine, from September 2023 to December 2024, following the approval of its extended care qualification. This study utilized a mixed-methods research design that integrates quantitative and qualitative approaches. The quantitative study included a total of 117 subjects, with an age range of 18 to 35 years, an average age of 21.56 years, and a median age of 19 years; there were 59 males and 58 females. The disease types covered four major categories: childhood leukemia and solid tumors (68 cases), congenital structural malformations (25 cases), congenital hereditary metabolic diseases (4 cases), and rare diseases (20 cases). Among the subjects, 57.26% (67 cases) were first-time visitors to SCMC. The patients came from 20 provinces, autonomous regions, and municipalities across the country, with 88.03% (103 cases) from outside Shanghai. The treatment outcomes showed improvement or cure in 80.34% (94 cases) of the subjects, and there were no medical complaints. In addition, a qualitative study was conducted to deeply explore the experiences, confusions, and challenges of receiving or implementing continuous medical services from the perspectives of patients and their families, as well as medical staff. According to the inclusion and exclusion criteria, a total of 44 subjects were included in the study, among them, there were 12 patients, 12 family members who were taking care of the patients in SCMC, and 20 corresponding medical staff members. The results of the qualitative study showed that trust in the attending physicians of the children's specialty hospital, a good doctor-patient relationship, satisfactory treatment outcomes, and support from medical insurance policies are the main driving forces for patients over 18 years old to receive continuous treatment at children's specialty hospitals. The medical staff of the hospital also believed that this model can promote patient benefits. In conclusion, under the policy support of the Shanghai Municipal Health Commission, the \"Six Fixed\" Model for continuous treatment established by SCMC has achieved certain positive results in practice. This provides practical references for the development of continuous treatment in China and offers new strategies for the application of preventive medicine in the field of children's health.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1170-1176"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and validation of the rapid health aging assessment scale for the Chinese population].","authors":"B Q Ye, J L Yang, J H Li, W N Chen, J H Ye, X T Zhou, Y Wang, S Q Li, Q Zhang, W Y Zhao, J Y Song, C Wang, Y Liu, M Xia","doi":"10.3760/cma.j.cn112150-20241121-00932","DOIUrl":"10.3760/cma.j.cn112150-20241121-00932","url":null,"abstract":"<p><p><b>Objective:</b> To develop a rapid assessment scale for healthy aging suitable for the Chinese population. <b>Methods:</b> Based on existing healthy aging assessment scales, national standards, and expert consensus, an initial Healthy Aging Rapid Assessment Scale was drafted through two rounds of expert consultation. A pre-survey was conducted with 3 220 subjects recruited from Guangzhou between July 2023 and July 2024. Items were screened through item analysis and exploratory factor analysis to form the final scale. Reliability and validity of the final scale were validated across five cities: Guangzhou, Dongguan, Shenzhen, Baoding, and Chuxiong. <b>Results:</b> The initial version comprised 36 items, while the finalized scale contained 18 items across three dimensions: metabolic health, mental health, and cognitive health. Test-retest reliability ranged from 0.71 to 0.81 across all study sites. The Spearman-Brown coefficient varied between 0.91-0.96, Cronbach's α between 0.77-0.83, comparative fit index (CFI) between 0.90-0.98, goodness-of-fit index (GFI) between 0.90-0.99, and root-mean-square error of approximation (RMSEA) between 0.03-0.09. For the three dimensions, reliability and validity metrics demonstrated consistency: Spearman-Brown coefficients 0.87-0.99, Cronbach's α 0.77-0.83, CFI 0.90-0.98, GFI 0.90-0.99, and RMSEA 0.03-0.09 across four regions. <b>Conclusion:</b> The developed Healthy Aging Rapid Assessment Scale for the Chinese population exhibits robust reliability and validity.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1078-1083"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of occupational burnout status and related factors among immunization program staff in Dezhou City, 2025].","authors":"L F Wang, X Yang, T T Lyu, Z Liu, A Q Xu","doi":"10.3760/cma.j.cn112150-20250306-00171","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250306-00171","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the current status and related factors of occupational burnout among immunization program staff in Dezhou City in 2025. <b>Methods:</b> From February to March 2025, 1 209 healthcare workers engaged in immunization programs in all disease prevention and control centers (CDCs), vaccination clinics and obstetrics vaccination rooms within the jurisdiction of Dezhou City were enrolled in this study. Basic information was collected via questionnaires. The Maslach Burnout Inventory-General Survey (MBI-GS) was used to assess occupational burnout status across three dimensions: emotional exhaustion, depersonalization and low personal achievement, with severity graded by scores. Multivariate logistic regression models were adopted to analyze the related factors of occupational burnout. <b>Results:</b> Among 1 209 participants, 986 (81.56%) were female and 513 (42.43%) were aged ≥40 years old. About 91 (7.53%) worked in CDCs, 970 (80.23%) in vaccination clinics and 148 (12.24%) in obstetric units. The detection rate of occupational burnout was 83.87% (1 014/1 209), with mild-to-moderate and severe occupational burnout rates of 16.38% (198/1 209) and 67.49% (816/1 209), respectively. Multivariate logistic regression model showed that compared with those who had no intention of resigning, those who frequently had turnover intention (<i>OR</i>=10.225, 95%<i>CI</i>: 4.093-25.543) had a higher risk of experiencing occupational burnout. Compared with those who slept for more than seven hours a day, those who slept for less than six hours a day (<i>OR</i>=4.266, 95%<i>CI</i>: 1.773-10.266) and those who slept for 6-7 hours (<i>OR</i>=1.543, 95%<i>CI</i>: 1.100-2.164) had a higher risk of developing occupational burnout. <b>Conclusion:</b> The detection rate of occupational burnout among immunization program staff in Dezhou City is relatively high, significantly related to frequent turnover intention and insufficient sleep.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1040-1046"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genomic characterization of a case of enterovirus D68 infection in a child from Tongzhou District, Beijing City].","authors":"B J Zhen, P Zhang, X C Guo, J Zhang, Y Zhang, X Gao, F Wang, J Li, L Zou","doi":"10.3760/cma.j.cn112150-20250326-00247","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250326-00247","url":null,"abstract":"<p><p>A throat swab sample from a pediatric case in Tongzhou District, Beijing was identified as enterovirus; the patient was a 1-year-and-8-month-old male sporadic case. Whole-genome sequencing revealed a viral genome length of 7 436 bp. BLAST alignment confirmed the serotype as EV-D68. Phylogenetic analysis of the whole genome indicated that this strain belongs to the B3 clade, showing closer genetic proximity to the 2018 Shanghai strain MW697453 with 99.53% whole-genome nucleotide homology. Genetic and amino acid variation analysis demonstrated that the B3 subclade to which this strain belongs exhibits a nucleotide deletion at positions 718-726, differing from deletion sites observed in other B3 clade strains. A key neuropathogenic amino acid site, T650A, was found to have undergone mutation. Recombination analysis confirmed no cross-clade recombination events in this strain. This study conducted genetic characterization of the strain's evolutionary relationship with EV-D68 strains from different regions and years in China, providing data support for formulating prevention and control measures against EV-D68 infection.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 7","pages":"1108-1112"},"PeriodicalIF":0.0,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}