中华预防医学杂志最新文献

{"title":"[Development and application of polysaccharide conjugate vaccine carrier protein].","authors":"J X Li, X Ma, A Lin, H X Pan, B Hao, J Shao, Y Z Li, Y T Xu, Z J Shao, A Q Xu","doi":"10.3760/cma.j.cn112150-20250326-00245","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250326-00245","url":null,"abstract":"<p><p>The development of polysaccharide conjugate vaccines, which convert polysaccharide antigens into T-cell-dependent immunogens through covalent conjugation with protein carriers, represents a critical strategy for enhancing immune protection in infants and young children. Globally licensed conjugate vaccines currently employ carrier proteins including Tetanus Toxoid (TT), Diphtheria Toxoid (DT), and Cross-Reacting Material 197 (CRM197). Recent advances have focused on three key areas: novel carrier protein discovery, optimized conjugation strategies, and evaluation of immune interference during co-administration of multivalent formulations. These efforts aim to achieve broader serotype coverage, prolonged protective efficacy, and simplified immunization schedules. This review synthesizes recent progress in carrier protein development, encompassing vaccine design principles, manufacturing processes, safety profiles, and epidemiological effectiveness. Furthermore, it critically examines current selection criteria for carrier proteins, their clinical applications, and persistent challenges, providing strategic insights to inform future conjugate vaccine development and immunization policy optimization in China.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of the trend and spatial clustering of lung cancer mortality in Shandong Province from 1970 to 2021].","authors":"Z T Fu, F Jiang, Z L Lu, J Chu, X H Xu, B Y Zhang, F Z Xue, X L Guo, A Q Xu, J X Ma","doi":"10.3760/cma.j.cn112150-202405310-00442","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-202405310-00442","url":null,"abstract":"<p><p><b>Objective:</b> To understand spatial aggregation of lung cancer mortality and its changing trends over the past fifty years in different counties and districts of Shandong Province from 1970 to 2021. <b>Methods:</b> The mortality data of lung cancer were obtained from the death registration system of Shandong province and three retrospective surveys of death cause. The mortality rate and age-standardized mortality rate were used to describe the changing trend of lung cancer in different years, and the contribution value of population factors and non-population factors in lung cancer mortality change was calculated by the mortality differential decomposition method. GeoDa 1.20 and ArcGIS 10.8 software were used for spatial autocorrelation analysis and visualization map display. <b>Results:</b> The crude mortality rate of lung cancer in Shandong Province showed a significant upward trend from 1970 to 2021, rising from 7.22 per 100 000 in 1970-1974 to 62.73 per 100 000 in 2020-2021, with an increase of 7.69 times. Meanwhile, the standardized mortality rate of lung cancer exhibited a trend of increasing first and then decreasing. The differential analysis of lung cancer mortality in different years revealed that changes in crude mortality rates were the result of the combined effects of demographic and non-demographic factors. The proportion of population factors (aging population) leading to an increase in lung cancer mortality rate rose from 2.12% in 1990-1992 to 40.20% in 2020-2021. From a spatial distribution perspective, there were significant regional differences in lung cancer mortality rates among counties (cities, districts) in Shandong Province across different eras. Compared to the period of 1970-1974, the lung cancer mortality rates in all counties and districts in 2020-2021 showed a considerable increase, and there were noticeable changes in the areas of high-high and low-low clustering of lung cancer mortality rates across different eras. <b>Conclusion:</b> There have been significant temporal and spatial changes in the mortality rate of lung cancer in Shandong Province from 1970 to 2021. The crude mortality rate has shown an upward trend, while the standardized mortality rate increases first and then decreases. The concentration of lung cancer mortality rates in counties and districts has also undergone significant changes.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"555-560"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Epidemiology clinical characteristics and risk factors analysis of severe rhinovirus associated pneumonia in children].","authors":"G Q Zhang, Y Zhang, Y J Wang, B Peng, C M Zhu","doi":"10.3760/cma.j.cn112150-20241212-01000","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20241212-01000","url":null,"abstract":"<p><p><b>Objective:</b> To explore the epidemiological characteristics, clinical manifestations, laboratory findings, and imaging features of children with HRV-associated pneumonia, and to analyze the clinical features and risk factors associated with severe HRV pneumonia, providing references for clinical management. <b>Methods:</b> A single-center, retrospective, observational study was conducted, including 1 001 cases of HRV-positive children with pneumonia admitted to the Respiratory Department of the Affiliated Children's Hospital of Capital Institute of Pediatrics from January 2019 to December 2023. Among them, 584 cases (58.3%) were male and 417 cases (41.7%) were female, with an age range of 0.1 to 14.9 years, a median age of 3.42 years, and a mean age of (3.92±2.75) years. According to clinical guidelines, the cases were divided into a mild pneumonia group (855 cases, 510 males, 345 females) and a severe pneumonia group (146 cases, 73 males, 73 females). Basic information, clinical, laboratory, and imaging data were collected from the electronic medical record system. Comparisons between different age groups, diagnoses, and pneumonia severity groups were performed using the <i>χ²</i> test. Multivariate logistic regression analysis was used to identify risk factors for the severity of HRV pneumonia. <b>Results:</b> Among the 1 001 cases of HRV-associated bronchopneumonia, 146 cases (14.6%) were severe pneumonia. The age of severe HRV pneumonia patients was significantly higher than that of the mild pneumonia group (5.2 years <i>vs.</i> 3.7 years, <i>t</i>=-6.050, <i>P</i><0.01). Severe HRV pneumonia had a higher incidence in autumn and winter (60.9%). Severe HRV pneumonia was associated with higher levels of lactate dehydrogenase (LDH), C-reactive protein (CRP), neutrophils, and creatinine (correlation coefficients 0.198, 0.334, 0.104, 0.142, <i>P</i><0.01), and lower levels of albumin (correlation coefficient 0.308, <i>P</i><0.01). Multivariate logistic regression analysis showed that co-infection with Streptococcus pneumoniae or Mycoplasma was an independent risk factor for severe HRV pneumonia [<i>OR</i>=1.611, 95% confidence interval (<i>CI</i>):1.066-2.435, <i>P</i><0.05; <i>OR=</i>3.355, 95%<i>CI</i>:2.062-5.458, <i>P</i><0.01]. <b>Conclusion:</b> The infection rate of HRV is higher in preschool and school-age children. Severe HRV pneumonia is associated with increased levels of LDH, CRP, neutrophils, and creatinine, as well as decreased levels of albumin. Co-infection with Streptococcus pneumoniae or Mycoplasma may be an independent risk factor for severe HRV pneumonia. High-risk children require enhanced monitoring and early intervention to improve prognosis.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"650-657"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of the trend and spatial clustering of esophageal cancer mortality in Shandong Province from 1970 to 2021].","authors":"Z L Lu, J Ren, J L Tang, J Chu, Z T Fu, F Jiang, X H Xu, X L Guo, A Q Xu","doi":"10.3760/cma.j.cn112150-20240918-00747","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240918-00747","url":null,"abstract":"<p><p><b>Objective:</b> To describe the distribution characteristics and trends of mortality and spatial aggregation of esophageal cancer in Shandong Province from 1970 to 2021. <b>Methods:</b> The mortality data of esophageal cancer were obtained from the death registration system of Shandong Province and three national all-cause mortality retrospective surveys. The crude mortality rate (CMR) and age-standardized mortality rate (ASMR, the Segi's world standard population) were used to describe the mortality of esophageal cancer. Mortality differential decomposition was applied to quantify the contributions of demographic and non-demographic factors. The death levels of esophageal cancer in different counties (cities and districts) in Shandong Province from 1970 to 1974 and 2020 to 2021 were visualized by the ArcGIS 10.8 software, and global and local autocorrelation analyses were conducted by using the GeoDa 1.12 software. <b>Results:</b> The CMR of esophageal cancer in Shandong Province increased first and then decreased from 1970 to 2021. The CMR of esophageal cancer decreased from 17.59/100 000 in the period of 1970-1974 to 14.32/100 000 in the period of 2020-2021. The ASMR of esophageal cancer decreased from 20.04/100 000 in the period of 1970-1974 to 6.53/100 000 in the period of 2020-2021. Compared with the period of 1970-1974, both demographic and non-demographic factors contributed to the increase in esophageal cancer mortality rate from 1990 to 1992. However, demographic factors continued to contribute to the increase in esophageal cancer mortality rate from 2004 to 2005, 2011 to 2013, and 2020 to 2021, while non-demographic factors contributed to the continuous decrease in esophageal cancer mortality rate. The global autocorrelation analysis results showed that the Moran's <i>I</i> index of ASMR of esophageal cancer in each county (city, district) of Shandong Province from 1970 to 1974 and from 2020 to 2021 were 0.67 and 0.57, respectively. Local autocorrelation analysis showed that there were 19 and 13 areas of high-high clustering of esophageal cancer in the periods of 1970-1974 and 2020-2021, respectively, with 12 overlapping counties (cities, districts). <b>Conclusion:</b> From 1970 to 2021, the CMR of esophageal cancer increases first and then decreases, while the ASMR of esophageal cancer gradually decreases in Shandong Province. The distribution of esophageal cancer mortality has significant spatial aggregation and changes over time.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"549-554"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A near-complete genomic analysis of aggregated outbreaks of norovirus subtype GⅡ.17[P17] in Beijing Chaoyang District from 2014 to 2024].","authors":"X Y Hu, J H Zhao, S Wang, X Qi, T L Han, Y H Yang, Y Gao, S Cong, L J Cao, L L Sun, M Jin, Y Jiao","doi":"10.3760/cma.j.cn112150-20241024-00847","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20241024-00847","url":null,"abstract":"<p><p><b>Objective:</b> To examine the near-complete genomic analysis of norovirus (NoV) subtype GⅡ.17 [P17] outbreaks in Beijing Chaoyang District from 2014 to 2024. <b>Methods:</b> Data and specimens related to outbreaks of the NoV aggregation in Beijing's Chaoyang District from 2014 to 2024 were collected. The NoV was identified using real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). Specimens with positive nucleic acid were amplified by standard PCR, whole genome sequencing and evolutionary analysis. Amino acid site variations were compared. <b>Results:</b> In Chaoyang District, from 2014 to 2024, a total of 637 aggregated outbreaks caused by the NoV infection were reported, of which 584 were successfully typed. The epidemic caused by the GⅡ.17 [P17] subtype accounted for 8.79% (56/637), which was the dominant epidemic gene subtype in 2014-2015, sporadic in 2016-2019, reappeared in 2022, and significantly increased in 2024 (27.27%, 24/88). Outbreaks caused by the GⅡ.17 [P17] subtype occurred mainly from October to December, with the main sites of occurrence in primary schools and kindergartens. This study yielded 53 near-complete genome sequences of the GⅡ.17 [P17] subtype from 46 incidents in Chaoyang District. The GⅡ.17 [P17] subtype sequences of Chaoyang District from 2014 to 2024 were segmented into three subgroups on the evolutionary tree, with sequences from 2014 to 2019, 2022 to April 2024, and May to December 2024 clustered into the d, e, and b subgroups, respectively. In the VP1 region's P2 area, particularly at the HBGA binding site, subgroups b and e exhibited mutations in 22 and two sites, while subgroups b and e showed mutations in four and one sites, predominantly in the RdRp region. <b>Conclusion:</b> The outbreak caused by the NoV GⅡ.17 [P17] subtype in Chaoyang District from 2014 to 2024 continues, with a significant increase in 2024, and it becomes the dominant gene subtype from October to December. The sequence formation of the NoV GⅡ.17 [P17] subtype in Chaoyang District from January to April 2022 and from May to December 2024 shows two different evolutions, with specific mutation sites, requiring continuous monitoring of the NoV GⅡ.17 [P17] subtype.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"640-649"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144081057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Minutes of the Vaccine Implementation Seminar Series held in Chongqing City].","authors":"Y Liu, X H Pang, Q Wang","doi":"10.3760/cma.j.cn112150-20250313-00201","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250313-00201","url":null,"abstract":"","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"747"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The roles of m6A-modified regulatory factors in the development of malignant tumors].","authors":"K He, X Zhou, J Li, J Q Wang, P Li, P Tan, X B Xie","doi":"10.3760/cma.j.cn112150-20241208-00985","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20241208-00985","url":null,"abstract":"<p><p>N6-methyladenosine (m6A) modification, as a widespread and high-profile type of epigenetic modification, can dynamically and reversibly regulate the whole process of RNA metabolism. This modification governs RNA stability, splicing, and translation via m6A regulators, which are categorized as Writers, Erasers, and Readers. m6A modification also plays a critical role in the development of tumors. Disruptions in the homeostasis of m6A modification levels lead to dysregulation of m6A regulators. Consequently, these dysregulated regulators modulate the proliferation, migration, and invasion of tumor cells and interfere with the normal functions of suppressor genes and oncogenes. This article aims to comprehensively elucidate the specific regulatory impacts of m6A modification on tumor-related gene expression. It focuses on the regulatory mechanisms of m6A modification on mRNA stability. Additionally, it explores the influence of m6A on the molecular translation of key signaling pathways. Moreover, it investigates the indirect regulatory effects mediated by non-coding RNAs (ncRNAs), etc. The intention is to offer a novel analysis of the pathogenesis of cancer at a new level, and also provide a theoretical basis for finding new effective treatment methods.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"716-727"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The mediating effect of electrocardiographic indicators in the association between exposure to fine particulate matter and its element constituents and blood pressure].","authors":"Y Wang, W W Zhang, Q Liu, H T Ling, C Z Xiang, Y Q Qiu, C Chen, J N Wang, J L Fang, X M Shi","doi":"10.3760/cma.j.cn112150-20241028-00852","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20241028-00852","url":null,"abstract":"<p><p><b>Objective:</b> To evaluate the mediating effect of electrocardiographic (ECG) indicators in the association between short-term exposure to fine particulate matter (PM_2.5) and blood pressure and to explore the key PM_2.5 element constituents that produce the mediating effect. <b>Methods:</b> Based on a cross-sectional survey across 10 cities in the Beijing-Tianjin-Hebei region and surrounding areas, PM_2.5 and its element constituents were collected from the nearest air monitoring superstation. Blood pressure and ECG indicators of participants were obtained through physical examinations. A multivariate linear regression was used to evaluate the effect of short-term exposures to PM_2.5 on blood pressure. A mediation analysis was used to identify the mediating effect of ECG indicators in the association between exposure to PM_2.5 and its element constituents and blood pressure. <b>Results:</b> The age of the 1 793 participants was (65.1±13.3) years, and 885 (49.4%) were males. During the study period, the daily mean concentration of PM_2.5 was (70±45) μg/m³, and the systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP) and pulse pressure (PP) were (139±20), (82±11), (101±13), and (57±17) mmHg (1 mmHg=0.133 kPa), respectively. The results of the multivariate linear regression showed that for every 10 μg/m³ increase in PM_2.5 on the same day (lag 0), DBP increased by 0.15 (95%<i>CI</i>: 0.02-0.28) mmHg, and PP decreased 0.18 (95%<i>CI</i>: 0.36-0.01) mmHg. The exposure to 14 elemental constituents, such as Ga, Co and Se, was associated with an increase in DBP, while the exposure to 17 elemental constituents, such as Cs, Se and Ag, was associated with a decrease in PP. At lag 0, the PM_2.5-induced increase in DBP was mediated by the QRS interval (mediation percentage of 18.98%), and the PM_2.5-induced decrease in PP was mediated by the QT interval (mediation percentage of -6.31%). The exposure to K, Br, Pb, Zn, Ca, Co, Pd, Cu, and As constituents was associated with increases in DBP mediated by prolonged QRS interval. The exposure to Pb, Zn, K, and As constituents was associated with decreases in PP mediated by prolonged QRS interval. <b>Conclusion:</b> ECG indicators such as QRS interval may mediate the association between short-term exposure to PM_2.5 and its element constituents and blood pressure.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"621-627"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Ursolic acid down-regulates ANXA6 and inhibits breast cancer cell growth and metastasis].","authors":"J W Qian, Z R Zhong, F F Xiang, M Z Zhang, G T Sun, R Wu","doi":"10.3760/cma.j.cn112150-20250314-00206","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20250314-00206","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the mechanism of ursolic acid (UA) in inhibiting the growth and metastasis of breast cancer MDA-MB-231 (\"231\") cells by downregulating ANXA6. &lt;b&gt;Methods:&lt;/b&gt; This study conducted relevant in vitro cytology and molecular biology experiments in the Department of Clinical Laboratory and Central Laboratory of Putuo Hospital, Shanghai University of Traditional Chinese Medicine from February 2023 to August 2024. Human breast cancer 231 cells were cultured in vitro, and the effects of different concentrations of UA on the proliferation and invasion and metastasis of 231 cells were detected by CCK-8 and Transwell assays. Western Blot was used to detect the effect of UA on the expression of ANXA6 and invasion and metastasis-related proteins MMP9, β-catenin and N-cadherin in 231 cells. The 231 cells that interfered with and overexpressed ANXA6 were constructed by lentivirus transfection to generate stable ANXA6 interfering and overexpressing 231 cells, which were divided into 231/KD-ANXA6 group, 231/KD-NC group, 231/OE-ANXA6 group, and 231/OE-NC group. CCK-8 assay and Transwell assay were used to detect the proliferation activity, invasion and metastasis ability of 231 cells after interference and overexpression of ANXA6 and the effect of UA on the proliferation ability of 231 cells after interference and overexpression of ANXA6. Western Blot and RT-PCR assays were used to detect the expression of invasion and migration biomarkers such as MMP9, β-catenin, and N-cadherin in 231 cells after interference and overexpression of ANXA6. Immunohistochemistry was used to detect the expression level of ANXA6 in breast cancer tissues, and the relationship between ANXA6 expression and clinicopathological features and prognosis of breast cancer was analyzed. &lt;b&gt;Results:&lt;/b&gt; The CCK-8 assay results showed that compared with the control group (0 μmol/L UA, 100.00%±7.37%), the proliferative activity of 231 cells at UA concentrations of 2.5, 5, 10, 20 and 40 μmol/L (90.23%±1.76%, &lt;i&gt;t&lt;/i&gt;=2.24, &lt;i&gt;P&lt;/i&gt;&lt;0.05; 85.19%±4.23%, &lt;i&gt;t&lt;/i&gt;=3.02, &lt;i&gt;P&lt;/i&gt;&lt;0.05; 65.45%±0.35%, &lt;i&gt;t&lt;/i&gt;=8.11, &lt;i&gt;P&lt;/i&gt;&lt;0.01; 37.79%±0.98%, &lt;i&gt;t&lt;/i&gt;=14.50, &lt;i&gt;P&lt;/i&gt;&lt;0.001; 18.18%±0.15%, &lt;i&gt;t&lt;/i&gt;=19.23, &lt;i&gt;P&lt;/i&gt;&lt;0.001) were significantly decreased. Furthermore, UA (10, 15, 20 μmol/L) inhibited the invasion and metastasis ability of 231 cells; Western Blot assay showed that compared with the control group (0 μmol/L UA), the protein expressions of MMP9 (1.07±0.03 &lt;i&gt;vs&lt;/i&gt; 0.99±0.11, &lt;i&gt;t&lt;/i&gt;=1.27, &lt;i&gt;P&lt;/i&gt;&gt;0.05), β-catenin (1.21±0.01 &lt;i&gt;vs&lt;/i&gt; 0.99±0.07, &lt;i&gt;t&lt;/i&gt;=5.47, &lt;i&gt;P&lt;/i&gt;&lt;0.05), N-cadherin (1.05±0.09 &lt;i&gt;vs&lt;/i&gt; 0.90±0.03, &lt;i&gt;t&lt;/i&gt;=2.65, &lt;i&gt;P&lt;/i&gt;&gt;0.05) at UA of 10 μmol/L; MMP9 (1.07±0.03 &lt;i&gt;vs&lt;/i&gt; 0.79±0.09, &lt;i&gt;t&lt;/i&gt;=5.26, &lt;i&gt;P&lt;/i&gt;&lt;0.001), β-catenin (1.21±0.01 &lt;i&gt;vs&lt;/i&gt; 0.89±0.05, &lt;i&gt;t&lt;/i&gt;=10.55, &lt;i&gt;P&lt;/i&gt;&lt;0.001), and N-cadherin (1.04±0.09 &lt;i&gt;vs&lt;/i&gt; 0.68±0.10, &lt;i&gt;t&lt;/i&gt;=4.59, &lt;i&gt;P&lt;/i&gt;&lt;0.05) at UA of 15 μmol/L; MMP9 (1.07±0.03 &lt;i&gt;vs&lt;/i&gt; 0.52±0.07, &lt;i&gt;t&lt;/i&gt;=12.50, &lt;i&gt;P","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"686-696"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Peri-coronary fat inflammation predicts proximal atherosclerotic plaque formation associated with LAD myocardial bridge].","authors":"S Y Li, F Zhou, Z H Xu, Y C Chen, Q Chen, Y Y Su, Y Feng, H T Zhu, L J Zhang","doi":"10.3760/cma.j.cn112150-20240709-00549","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240709-00549","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the correlation between peri-coronary fat attenuation index (FAI) and plaque formation in patients with myocardial bridge (MB) of the left anterior descending artery (LAD) using coronary computed tomography angiography (CCTA) and to develop an optimal predictive model to explore the potential application of FAI in the primary prevention of MB related atherosclerosis. <b>Methods:</b> In this retrospective study, prediction models associated with perivascular fat inflammation were developed and validated using both logistic regression and machine learning (ML) algorithm. A training dataset was collected from 253 patients who underwent ≥2 coronary computed tomography angiography (CCTA) with ≥3 months intervals from one tertiary hospital from January 2007 to April 2021 and had baseline CCTA showing no plaques in LAD MB. The median follow-up time was 3.2 years. According to the same criteria, a total of 75 LAD MB patients from four other hospitals were included to form an independent external validation dataset, with a median follow-up time of 1.8 years. Receiver operating characteristic (ROC) curve analysis with integrated discrimination improvement (IDI) and category net reclassification index (NRI) were used to compare the performance of the predictive models. <b>Results:</b> 62 patients (24.5%) in the training dataset had proximal plaque formation in LAD MB, while 22 patients (29.3%) in the external validation dataset had plaque formation during the follow-up period. Baseline FAI within the longitudinal distance equal to 30 mm proximal to the MB entrance was an independent predictor (<i>OR=</i>1.068, <i>P</i>=0.046). According to the model results, ROC curves were plotted. The AUC of Model 1 was 0.822, and the AUCs of Model 2 and 1 were 0.821 and 0.591 in the training dataset. After the DeLong test, the AUC of Model 1 was superior to that of Model 2 (<i>Z</i>=2.839, <i>P</i>=0.005) and Model 1 (<i>Z</i>=6.124, <i>P<</i>0.001). These findings were further validated in the external validation dataset, where ML-model 3 yielded the best predictive performance, outperforming the logistic regression-based Model 2 (categorical <i>NRI</i>=0.359, <i>P</i>=0.048; <i>IDI</i>=0.108, <i>P</i>=0.046). <b>Conclusion:</b> FAI measured within the 30 mm proximal to the entrance of MBs due to its prone to plaque development is an independent predictor for atherosclerotic plaque formation. The ML-prediction model based on a decision tree algorithm combines FAI, MB anatomical features, and patient risk factors, which is beneficial for patients undergoing routine CCTA examination to identify inflamed coronary arteries in advance and guide the clinical adoption of more targeted preventive treatment, including anti-inflammatory treatment.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"59 5","pages":"604-612"},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}