Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology最新文献_第6页

[Laboratory evaluation of automated enzyme linked fluorescent assay for detecting serum specific IgG antibodies against rubella virus]. [自动酶联荧光法检测风疹病毒血清特异性IgG抗体的实验室评价]。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1996-02-01

T C Hou, W K Huang, Y C Liu

{"title":"[Laboratory evaluation of automated enzyme linked fluorescent assay for detecting serum specific IgG antibodies against rubella virus].","authors":"T C Hou, W K Huang, Y C Liu","doi":"","DOIUrl":"","url":null,"abstract":"The VIDAS rubella IgG(RBG) is a new, automated, enzyme-linkad fluorescent assay(ELFA) for detecting IgG antibodies to rubella virus in serum. The purpose of this study was to evaluate the usefulness of the qualitative and quantitative VIDAS RBG as laboratory tests in the rapid detecting anti-rubella IgG antibodies. Simultaneous parallel testing was performed by using the qualitative and quantitative RBG along with RUBAZYME(ABBOTT LABORATORIES) and the standard hemagglutination-inhibition(HI) test(R-HI kit, SEIKEN) on 200 blood samples submitted for anti-rubella IgG antibodies testing from patients at the VGH-Kaohsiung(Mar.-Sep., 1993). The qualitative and quantitative VIDAS RBG and RUBAZYME assay were compared to the standard R-HI test. Significant differences among the sensitivities of these three methods were found (100% sensitivity for the qualitative VIDAS RBG, 88.89% for the quantitative VIDAS RBG and 97.67% for the RUBAZYME). However, the specificities of these three methods were all the same, 100%. With these results we can conclude that the qualitative VIDAS RBG will provide a very good, precise and reliable method to determine serum specific IgG antibodies against rubella virus. Furthermore, the VIDAS RBG is fully automated and time-saving procedures (for every batch of test, it takes 25 minutes with VIDAS RBG, 2.5 hours with RUBAZYME and 4.5 hours with R-HI) in clinical laboratories.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21450987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribotyping of clinical Vibrio vulnificus isolates. 临床创伤弧菌分离株的核分型研究。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

S F Yang, J C Cheng, L I Hor

引用次数: 0

Molecular epidemiological studies of Vibrio cholerae in Taiwan: genotyping by polymerase chain reaction and DNA sequencing. 台湾霍乱弧菌分子流行病学研究:聚合酶链反应基因分型及DNA测序。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

D P Liu, P J Chen, C S Lin, T N Wu

{"title":"Molecular epidemiological studies of Vibrio cholerae in Taiwan: genotyping by polymerase chain reaction and DNA sequencing.","authors":"D P Liu, P J Chen, C S Lin, T N Wu","doi":"","DOIUrl":"","url":null,"abstract":"To type the Vibrio cholerae strains isolated from sporadic and epidemic cases in Taiwan, 28 toxigenic isolates were studied by sequencing polymerase chain reaction-amplified cholera toxin gene (ctx) fragments. Based on specific base substitutions on positions 115 and 203 of ctxB and comparison with previously published typing system from Centers for Disease Control and Prevention (Olsvik theta et al., J Clin Microbiol 1993; 31:22-5, Ref.1), two genotypes were identified. Cholera strains from imported seafood and sporadic cases in Taiwan had ctxB polymorphism of genotype 1; strains from patients in the 1962 Taiwan epidemic and Taiwan raised soft-shell turtles had ctxB polymorphism of genotype 3. Moreover, one toxigenic non-O1 strain was found to have other 11 different nucleotides in ctxB compared with those of the O1 and O139 strains. Therefore, DNA sequencing is a useful method for obtaining more complete genetic information. The approach could be improved by applying it to other more polymorphic regions of bacterial genome to obtain better epidemiological information among infected cases.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the manganese-resistant mutants derived from Vibrio parahaemolyticus. 副溶血性弧菌锰抗性突变体的鉴定。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

J H Weng, H C Wong, C C Liu, S Y Lee, C M Yu, Y S Lee, H C Tseng, K Y Lu

引用次数: 0

Sequencing analysis of hepatitis C virus mixed genotype in a hemodialysis patients. 1例血液透析患者丙型肝炎病毒混合基因型序列分析。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

H F Lee, H Y Lu, H L Hsiao, C B Horng

{"title":"Sequencing analysis of hepatitis C virus mixed genotype in a hemodialysis patients.","authors":"H F Lee, H Y Lu, H L Hsiao, C B Horng","doi":"","DOIUrl":"","url":null,"abstract":"It has become clear that the genotypes of HCV vary with respect to pathogenicity, infectivity, response to antiviral therapy and geographic clustering. The prevalence of genotype distribution of HCV infection in Taiwan was investigated by typing with type-specific DNA primers in HCV core region. Using a design by Okamoto et al., it was found that in 280 serum samples examined, 3.3% (4/122) of the virus detected were mixed type. The implication of mixed type infection remains to be clarified: whether it is a single infection with a new variant, or infection with two HCV virions at different times or confusion with type-specific DNA primers themselves. The nucleotide sequences of the recombinant plasmid DNAs and the PCR products recovered from gel electrophoresis were analyzed by autosequencer. Gene sequences of HCV cDNAs of the two blood donors were used as control. To double check the results, we have also analyzed the DNA sequences of the cloned plasmids in the NS5 region with the primer system designed by Chayama et al. Results indicated that the hemodialysis patient was doubly infected with HCV, rather than by a HCV variant.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of antitrypanosome antibodies and studies of their specificity and autoimmunity. 抗锥虫抗体的概况及其特异性和自身免疫的研究。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

D Chao, D G Dusanic, K W Yu

{"title":"Survey of antitrypanosome antibodies and studies of their specificity and autoimmunity.","authors":"D Chao, D G Dusanic, K W Yu","doi":"","DOIUrl":"","url":null,"abstract":"As a part of investigations to characterize trypanosome infections in Taiwan, sera collected from patients admitted to Veterans General Hospital, Taipei were tested for antitrypanosome antibodies. A Trypanosoma cruzi extract-based enzyme-linked immunosorbent assay (ELISA) was used to screen and titrate 1,297 patient sera. High antitrypanosome titers were detected in 166 (12.8%) of these sera. Retitration of random samples of the high titer (HT) sera indicated a 5.4% false positive. Thirteen donors with high antitrypanosome ELISA titers were followed up. Twelve of then remained high serum titers also showed high ELISA titers against an extract of Trypanosoma conorhini. Hemocultures conducted on freshly drawn blood specimens of the 13 subjects did not provide any evidence of trypanosome infections. Electrophoretic analyses of sera from HT and low titer (LT) patients suggested differences between serum proteins of the subjects in each of the groups. Atypical reactions were observed in immunodiffusion tests performed with HT and LT sera and trypanosome extracts, while western blot analyses revealed a complex pattern of binding by both sera. The qualitative and quantitative differences in these tests suggested interactions of T. cruzi antigens with donor antibodies against unrelated antigens and/or with autoantibodies. Subsequent analyses did not indicate any association between rheumatoid factor and the reactivities of the HT sera with the parasites. However, antinuclear antibodies were detected with an indirect fluorescent antibody test (IFAT) in 50% of the HT sera and 22% of the LT sera. No differences were found between the levels of antilaminin activity of the two groups. The IFAT employing T. cruzi epimastigotes was positive for 100% of the HT sera and 22% of the LT sera. The data indicate that the high seropositivity recognized in this study is due in part to the activities of cross-reacting antibodies and/or autoantibodies in the sample population.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of Streptococcus mutans glucosyltransferase (GtfC) expressed in Escherichia coli. 在大肠杆菌中表达的变形链球菌葡萄糖基转移酶(GtfC)的纯化和鉴定。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-11-01

J S Chia, C C Hsieh, C S Yang, J Y Chen

{"title":"Purification and characterization of Streptococcus mutans glucosyltransferase (GtfC) expressed in Escherichia coli.","authors":"J S Chia, C C Hsieh, C S Yang, J Y Chen","doi":"","DOIUrl":"","url":null,"abstract":"Streptococcus mutans constitutively expresses three glucosyltransferases, i.e., GtfB, GtfC, and GtfD; which synthesize glucan polymers from sucrose. To obtain individual GTF without complexing with one another, a purification strategy was developed to recover recombinant GTF expressed from Escherichia coli. The recombinant GtfC was aggregated and associated with the insoluble fraction in E. coli homogenates. GtfC was solublized with the 8M urea, renatured to its biologically active form by serial dialysis against sodium phosphate buffer, and subsequently purified to homogeneity by DEAE-Sephacel and hydroxylapatite column chromatography. The GtfC enzyme preparation was purified 16.3-fold and the molecular weight was estimated to be 140 kDa. GtfC synthesized water insoluble glucan in a primer independent manner and its enzymatic activities could be enhanced by dextran. Purified GtfC had a pH optimum of 6.5, a K(m) of 9.26 mM for sucrose and a pI of 5.5. Distinct from the previous reports, results from this study offers an alternative for the purification of the recombinant GTFs free from any detergent contamination to make it more suitable for utilization in vivo.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HLA-A, B antigens and their linkage with HLA-DR among blood donors in Taiwan. 台湾献血者HLA-A、B抗原及其与HLA-DR的关系。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-08-01

S J Tsai, C M Yang, H L Kuan, B S Yang, K S Lin

引用次数: 0

In Vitro antimicrobial susceptibilities of Streptococcus pneumoniae isolated from two teaching hospitals in Taiwan, 1989-1995. 1989 ~ 1995年台湾两所教学医院分离的肺炎链球菌体外抗菌药物敏感性分析。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-08-01

J Y Su, S C Chang, K T Luh, W C Hsieh

引用次数: 0

Biological characteristics of human hepatocellular carcinoma cell lines with or without prior nude mouse passages. 人肝癌细胞系经或未经裸鼠传代的生物学特性。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-08-01

K S Chang, M J Chou, S Tsai, C S Yang

{"title":"Biological characteristics of human hepatocellular carcinoma cell lines with or without prior nude mouse passages.","authors":"K S Chang, M J Chou, S Tsai, C S Yang","doi":"","DOIUrl":"","url":null,"abstract":"A cell line, J5, derived directly from the human hepatocellular carcinoma (HCC) by in vitro culture, and three other lines, J2-23, J3-27 and J3-28, which were established in culture only after passages in nude mice were examined. Since nude mice as well as various strains of normal mice often produce infectious murine retroviruses, the HCC cells passaged as a heterotransplant in nude mice may be infected with these viruses. The supernatant of the J2-23, J3-27 and J3-28 cell co-cultured with a wild mouse cell line, SC-1, which supports the murine ecotropic retroviruses, showed the presence of a murine N-tropic virus. No murine xenotropic retrovirus was detected in these cells using S+L- mink cell assay method. A clone (J3-28 Clone 1) was derived from the above mentioned J3-28 cells, since the latter contained a mixture of human and murine cells. The J3-28 Clone 1 was found to be entirely of human karyotype. This clone as well as the J5 and the PLC/PRF/5 cel lines which have never been passaged in nude mice, showed none of these murine retroviruses. By Southern-blot hybridization, no change was detectable for c-abl, c-ras, c-mos, and c-myc protooncogenes in the chromosomal DNA of J2-23, J3-27, J3-28 Clone 1, and J5 cell lines. The karyotypes of all the HCC cell lines were aneuploid, and those of the J2-23 and J3-27 cell lines were of mouse, while the J3-28 Clone 1 and J5 cell lines were of human. The chromosomes of the J2-23 and J3-27 cells showed, except for the presence of a few marker chromosomes in the former, no apparent changes in the length were observed. In contrast, those of the J3-28 Clone 1 and J5 cells exhibited various changes, including 6-8 marker chromosomes, and an increase or decrease in the length of p and/or q arms of various chromosomes.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0