Biological characteristics of human hepatocellular carcinoma cell lines with or without prior nude mouse passages.

K S Chang, M J Chou, S Tsai, C S Yang
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Abstract

A cell line, J5, derived directly from the human hepatocellular carcinoma (HCC) by in vitro culture, and three other lines, J2-23, J3-27 and J3-28, which were established in culture only after passages in nude mice were examined. Since nude mice as well as various strains of normal mice often produce infectious murine retroviruses, the HCC cells passaged as a heterotransplant in nude mice may be infected with these viruses. The supernatant of the J2-23, J3-27 and J3-28 cell co-cultured with a wild mouse cell line, SC-1, which supports the murine ecotropic retroviruses, showed the presence of a murine N-tropic virus. No murine xenotropic retrovirus was detected in these cells using S+L- mink cell assay method. A clone (J3-28 Clone 1) was derived from the above mentioned J3-28 cells, since the latter contained a mixture of human and murine cells. The J3-28 Clone 1 was found to be entirely of human karyotype. This clone as well as the J5 and the PLC/PRF/5 cel lines which have never been passaged in nude mice, showed none of these murine retroviruses. By Southern-blot hybridization, no change was detectable for c-abl, c-ras, c-mos, and c-myc protooncogenes in the chromosomal DNA of J2-23, J3-27, J3-28 Clone 1, and J5 cell lines. The karyotypes of all the HCC cell lines were aneuploid, and those of the J2-23 and J3-27 cell lines were of mouse, while the J3-28 Clone 1 and J5 cell lines were of human. The chromosomes of the J2-23 and J3-27 cells showed, except for the presence of a few marker chromosomes in the former, no apparent changes in the length were observed. In contrast, those of the J3-28 Clone 1 and J5 cells exhibited various changes, including 6-8 marker chromosomes, and an increase or decrease in the length of p and/or q arms of various chromosomes.

人肝癌细胞系经或未经裸鼠传代的生物学特性。
通过体外培养直接从人肝细胞癌(HCC)中获得的细胞系J5和裸鼠传代后在培养中建立的细胞系J2-23、J3-27和J3-28。由于裸鼠和不同品系的正常小鼠经常产生感染性小鼠逆转录病毒,在裸鼠中异种移植传代的HCC细胞可能感染这些病毒。J2-23、J3-27和J3-28细胞与支持小鼠嗜生态逆转录病毒的野生小鼠细胞系SC-1共培养的上清液显示存在小鼠嗜n病毒。S+L-水貂细胞法未检测到小鼠异向性逆转录病毒。克隆(J3-28克隆1)来源于上述J3-28细胞,因为后者包含人类和小鼠细胞的混合物。J3-28克隆1完全符合人类核型。该克隆以及从未在裸鼠中传代过的J5和PLC/PRF/5细胞系均未显示出上述小鼠逆转录病毒。通过Southern-blot杂交,J2-23、J3-27、J3-28克隆1和J5细胞系染色体DNA中c-abl、c-ras、c-mos和c-myc原癌基因未见变化。所有HCC细胞系的核型均为非整倍体,其中J2-23和J3-27细胞系为小鼠核型,J3-28克隆1和J5细胞系为人核型。J2-23和J3-27细胞的染色体长度除前者存在少量标记染色体外,未见明显变化。而J3-28克隆1和J5细胞则表现出不同程度的变化,包括6-8条标记染色体,以及各种染色体的p臂和/或q臂长度的增加或减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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