中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20230919-00061
W K Li, Y Zhang, X Y Qu, Y Q Lin, Y Z Zhao, N Liu
{"title":"[N-acetylcysteine regulates NF-κB signaling pathway alleviates the pulmonary toxicity induced by indium-tin oxide nanoparticles in rats].","authors":"W K Li, Y Zhang, X Y Qu, Y Q Lin, Y Z Zhao, N Liu","doi":"10.3760/cma.j.cn121094-20230919-00061","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20230919-00061","url":null,"abstract":"<p><p><b>Objective:</b> The current study aimed to evaluate the possible protective effects of N-acetylcysteine (NAC) against Indum-tin oxide (ITO) nanoparticle (Nano-ITO) -induced pulmonary alveolar proteinosis (PAP) in rats, especially via modulation of nuclear factor kappa B (NF-κB) signaling. <b>Methods:</b> In October 2019, 50 adult male Sprague-Dawley rats were randomly allocated into five groups (10 rats each) as follows: blank control group, saline control group, NAC control group (200 mg/kg), Nano-ITO group (receiving a repeated intratracheal dose of 6 mg/kg Nano-ITO) and NAC intervention group (pre-treated intraperitoneally with 200 mg/kg NAC 1.5 h before the administration of an intratracheal dose of 6 mg/kg Nano-ITO). The rats were exposed twice a week for 12 weeks. Rats were then euthanized under anesthesia, and their lungs were removed for histopathological and immunohistochemical analysis. The comparison of indicators reflecting oxidative stress and pulmonary inflammation among groups was conducted using one-way analysis of variance (ANOVA) and Bonferroni's test. The effect of NAC on Nano-ITO induced NF-κB signaling pathway in rats was analyzed. <b>Results:</b> Histopathological examination of Nano-ITO exposed rats revealed diffuse alveolar damage, including PAP, cholesterol crystals, alveolar fibrosis, pulmonary fibrosis, and alveolar emphysema. Immunohistochemical results of Nano-ITO exposed rats showed strong positive for nuclear factor κB p65 (NF-κB p65) and nuclear factor Kappa B inhibitory factor kinase (IKK-β) and weak positive for nuclear factor κB inhibitory protein α (IκB-α) in the nuclei of bronchiolar and alveolar epithelial cells. Compared with blank control group, saline control group and NAC control group, the level of total protein (TP) in bronchoalveolar lavage fluid of rats in Nano-ITO group was significantly increased (<i>P</i><0.05), and the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD), malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were significantly increased (<i>P</i><0.05), the levels of proinflammatory cytokines interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) were significantly increased (<i>P</i><0.05), and the levels of NF-κB p65, IKK-β, inducible nitric oxide synthase (iNOS) and reactive oxygen species (ROS) in lung tissue were significantly increased (<i>P</i><0.05). Compared with Nano-ITO group, the levels of TP, T-AOC, MDA and TNF-α in bronchoalveolar lavage fluid of rats in NAC intervention group were significantly decreased (<i>P</i><0.05), and the levels of NF-κB p65 and ROS in lung tissue were significantly decreased (P<0.05). Western blot results showed that compared with the control groups, the protein expressions of NF-κB p65 and IKK-β in the lung tissue of Nano-ITO group were increased, while the protein expression of IκB-α was decreased (<i>P</i><0.05). Compared with Nano-ITO group, the protein expressions of ","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"721-729"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20231116-00119
Y M Zheng, W Liu, L Guan
{"title":"[The occupational exposure prevention and control system for hazardous drugs in the United States].","authors":"Y M Zheng, W Liu, L Guan","doi":"10.3760/cma.j.cn121094-20231116-00119","DOIUrl":"10.3760/cma.j.cn121094-20231116-00119","url":null,"abstract":"<p><p>Hazardous drugs represented by antineoplastic drugs have clear health hazards to occupational exposed populations, and this issue has received widespread attention all over the world in recent years. This article reviewed the laws, regulations, guidelines, and industry guidance documents for the prevention and control of occupational exposure to hazardous drugs issued by relevant departments and associations in the United States. It could provide a reference basis for the development of occupational environmental health standards and protection guidelines, with the aim of reducing the risk of occupational exposure to hazardous drugs and promoting the healthy development of healthcare services in China.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"779-782"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20240118-00027
J M Wang, W Qin, W L Xie
{"title":"[Analysis of personal dose monitoring results of occupational external exposure for radiation workers in a hospital].","authors":"J M Wang, W Qin, W L Xie","doi":"10.3760/cma.j.cn121094-20240118-00027","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240118-00027","url":null,"abstract":"<p><p><b>Objective:</b> To analyze and understand the level of occupational external radiation personal dose of radiation workers in a hospital, and provide a reference for radiation protection. <b>Methods:</b> In May 2023, the personal dose monitoring data of all radiation workers in the hospital from 2018 to 2022 were collected, and their occupational external radiation exposure levels were analyzed and evaluated. All radiation workers were divided into radiation diagnosis, radiotherapy, interventional radiology, and nuclear medicine according to their occupational categories. <b>Results:</b> A total of 1626 person-times were monitored from 2018 to 2022, and the average annual effective dose per person (0.13-0.36 mSV) was lower than the national limit of 20 mSv. The average annual dose of nuclear medicine was the highest (0.36 mSv), and interventional radiology was lower (0.13 mSv). The average annual dose of individuals was the highest in 2020 (0.24 mSv) . <b>Conclusion:</b> The average annual effective dose per person of radiation workers in this hospital is lower than the national standard, and it is still necessary to strengthen the inspection of the wearing of personal dosimeters and the using of protective equipment for radiation workers.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"762-764"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20230609-00200
Y F Bian, H C Zhong, Q Jin, P P Pan
{"title":"[Analysis of cytogenetic indexes and occupational characteristics of radiation workers with lens opacity].","authors":"Y F Bian, H C Zhong, Q Jin, P P Pan","doi":"10.3760/cma.j.cn121094-20230609-00200","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20230609-00200","url":null,"abstract":"<p><p><b>Objective:</b> To analyse the characteristics of micronuclus and chromosomal aberrations and the lens opacity in different working years, ages and work type of radiation workers with lens opacity. <b>Methods:</b> In January 2022, we selected 400 radiation workers with lens opacity who had undergone occupational health examination in Hangzhou Hospital for the Prevention and Treatment of Occupational Disease from March 2014 to December 2021 as research objects. To analyze the differences of micronucleus, chromosome aberration and the lens opacification rates of different length of service, type of work, age radiologic workers. <b>Results:</b> The results showed that radiation the micronucleus abnormality (0.59‰) and chromosome aberration (2.67%) in peripheral blood in workers who served more than 20 years were significantly higher than that of micronucleus abnormality (0.18‰) and chromosome aberration (0.31%) in workers served less than 20 years, the differences were statistically significant (<i>P</i><0.05). The micronucleus abnormality (0.32‰) and chromosome aberration (0.57%) in the interventionist were higher than oral radiation workers (0.12‰ and 0.19%) and diagnostic radiologist (0.05‰ and 0.12%), the differences were statistically signcficant (χ(2)=23.98, 6.72, <i>P</i><0.05) . <b>Conclusion:</b> The rates of micronucleus and chromosome aberration of the interventionaist are higher than oral radiation workers and the traditional radiologist. Workers engaged in radiation should improve personal protection and undertake regular occupational health examinations.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"743-745"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20240109-00119
L Chen, Y Liao, Z B Cen, J W Zhang, M H Xiao, Z Wang
{"title":"[Analysis of disease burden and trends of noise-induced hearing loss in China from 1990 to 2019].","authors":"L Chen, Y Liao, Z B Cen, J W Zhang, M H Xiao, Z Wang","doi":"10.3760/cma.j.cn121094-20240109-00119","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20240109-00119","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the disease burden of Noise-Induced Hearing Loss (NIHL) in China from 1990 to 2019, forecast the disease burden of NIHL from 2020 to 2030, and provide data support for the prevention and control of NIHL. <b>Methods:</b> In July 2022, the disease burden data of NIHL in different age groups and genders in China during 1990-2019 were selected from the GBD database. The Jointpoint regression model was established to analyze the trend of the disease burden of NIHL in China. An age-period-cohort model was constructed to analyze the changing trend of NIHL in terms of age, period, and cohort, and a Bayesian age-period-cohort model was developed to predict the disease burden of NIHL in China from 2020 to 2030. <b>Results:</b> From 1990 to 2019, the disability adjusted life year (DALY) of China's NIHL increased from 1361600 to 2327700 years. The coarse rate of DALY increased from 115.03/100000 to 163.65/100000 (AAPC=1.23, <i>P</i><0.001), and the normalization rate of DALY decreased from 127.67/100000 to 119.83/100000 (AAPC=-0.21, <i>P</i><0.001). It is predicted that from 2020 to 2030, the DALYs of China's NIHL will increase from 2412900 in 2020 to 2655000 in 2030, and the DALY normalization rate will decrease from 241.29/100000 in 2020 to 125.71/100000 in 2030. <b>Conclusion:</b> The burden of noise-induced hearing loss (NIHL) in China is significant and should not be overlooked. To reduce this burden, we need to focus on strengthening source management, process control, personal protection, and comprehensive prevention and treatment through various methods.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"730-734"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20231106-00101
K Yang, Hogahemat Xiao Kelaiti, P Peng
{"title":"[Current status of research on the diagnosis and treatment of acute ethylene glycol poisoning].","authors":"K Yang, Hogahemat Xiao Kelaiti, P Peng","doi":"10.3760/cma.j.cn121094-20231106-00101","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231106-00101","url":null,"abstract":"<p><p>Acute ethylene glycol poisoning often leads to acute kidney injury, and delays in diagnosis and treatment can lead to severe multi-organ dysfunction and even death. At present, there is a lack of specific laboratory tests that can be used for rapid clinical diagnosis, and diagnosis and evaluation only rely on medical history, clinical symptoms, and non-specific laboratory test results. Key treatments include alcohol dehydrogenase inhibitors (fomcpizole or ethanol) and hemodialysis, which is not approved in China. A clear understanding of the mechanism of toxicity, pathophysiology, clinical manifestations, efficacy and limitations of laboratory tests, and treatment options after ethylene glycol poisoning can help accelerate the early diagnosis, treatment, and prognosis of patients after ethylene glycol poisoning. This article reviews this article in order to provide a reference for clinicians.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"788-793"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20230926-00070
Z L Chen, J C Li, L Wu, X Y Dai, S Q Chen, L Yang
{"title":"[Diagnosis and management of work related musculoskeletal disorders as occupational diseases in Germany].","authors":"Z L Chen, J C Li, L Wu, X Y Dai, S Q Chen, L Yang","doi":"10.3760/cma.j.cn121094-20230926-00070","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20230926-00070","url":null,"abstract":"<p><p>This article introduces the occupational disease diagnosis of work-related musculoskeletal disorders such as lumbar and spinal injuries in Germany, and focuses on the research and standard values of the maximum pressure tolerance of intervertebral discs. This article mainly introduces the dose model limits and their applications proposed by the Mainz Dortmund Dosis model (MDD), the judgment of the German Social Court (Bundessozialgericht, BSG), and the German Spinal Research Re evaluation (Deutsche Wirbels ulentudieⅡ, DWSⅡ) ; X-ray manifestations and measurements of intervertebral disc injury; Problems in actual cases and diagnosis. In order to provide reference and inspiration for the occupational diseases diagnosis of work related musculoskeletal disorders in China.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"783-787"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20230713-00247
Z P Dong, Z M Fan, H Y Shao, Y X Zhao
{"title":"[A case report of pulmonary aspergillosis secondary to occupational chronic formaldehyde toxic obstructive pulmonary disease].","authors":"Z P Dong, Z M Fan, H Y Shao, Y X Zhao","doi":"10.3760/cma.j.cn121094-20230713-00247","DOIUrl":"10.3760/cma.j.cn121094-20230713-00247","url":null,"abstract":"<p><p>Invasive pulmonary aspergillosis is the most common type of pulmonary aspergillosis. This paper reported a patient with pulmonary aspergillosis secondary to obstructive pulmonary disease and other underlying diseases. The clinical manifestations included wheezing, cough, fever and wheezing rale in the lungs. Diagnosis was ultimately confirmed through pathogens targeted next generation sequencing and pathological examination of respiratory coughs. Following comprehensive treatment that included antifungal therapy, the patient was cured and discharged with a good prognosis.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"775-778"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-10-20DOI: 10.3760/cma.j.cn121094-20231024-00093
J Pan, M C Wang, S Y Zhao, Q Liu, H Y Zhai
{"title":"[A meta-analysis of the prevalence of work-related musculoskeletal disorders among rehabilitation therapists].","authors":"J Pan, M C Wang, S Y Zhao, Q Liu, H Y Zhai","doi":"10.3760/cma.j.cn121094-20231024-00093","DOIUrl":"10.3760/cma.j.cn121094-20231024-00093","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the prevalence of work-related musculoskeletal disorders (WMSDs) in rehabilitation therapists. <b>Methods:</b> In September 2023, the computer searched Chinese and English databases such as CNKI database, Wanfang Data Knowledge Service Platform, VIP Chinese Science and Technology Journal Database, Chinese Biomedical Literature Database, PubMed, Cochrane Library, Web of Science, Embase database, etc. The retrieval time was from the establishment of the database to September 2023. Musculoskeletal disorders, rehabilitation therapists were selected as search terms, and the author, publication time, prevalence rate, and study type were analyzed. The Agency for Healthcare Research and Quality (AHRQ) tool and The Newcastle-Ottawa Scale (NOS) were used to evaluate the quality of the literature. Stata 15.0 software was used for meta-analysis. <b>Results:</b> A total of 28 literatures were included, including 8039 rehabilitation therapists. Meta-analysis showed that the overall prevalence rate of WMSDs among rehabilitation therapists was 72% (95%<i>CI</i>: 64%-80%). The top 3 annual prevalence rates were lower back (51%, 95%<i>CI</i>: 38%-64%), neck (42%, 95%<i>CI</i>: 30%-54%) and shoulder (32%, 95%<i>CI</i>: 23%-41%). The top 3 weekly prevalence rates were lower back (47%, 95%<i>CI</i>: 28%-65%), neck (35%, 95%<i>CI</i>: 14%-55%) and back (30%, 95% <i>CI</i>: 17%-44%) . <b>Conclusion:</b> The higher prevalence of WMSDs in rehabilitation therapists, especially in the lower back, neck, shoulder and back, may be related to the prolonged stouching posture and the continuous or repeated exertion of the upper limbs.</p>","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 10","pages":"746-752"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142547867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
中华劳动卫生职业病杂志Pub Date : 2024-09-20DOI: 10.3760/cma.j.cn121094-20231010-00080
T Meng, H J Guo, Y Yao, Z H Mi, Y Tian, J Z Yu
{"title":"[Study on the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells and the intervention effect of N-acetylcysteine].","authors":"T Meng, H J Guo, Y Yao, Z H Mi, Y Tian, J Z Yu","doi":"10.3760/cma.j.cn121094-20231010-00080","DOIUrl":"https://doi.org/10.3760/cma.j.cn121094-20231010-00080","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the molecular mechanism of autophagy and apoptosis induced by ultrafine carbon black in human bronchial epithelial cells (BEAS-2B cells), and to study the intervention effect and mechanism of N-acetylcysteine (NAC) on ultrafine carbon black-induced oxidative damage in BEAS-2B cells. <b>Methods:</b> In March 2023, BEAS-2B cells were used as research object, an in vitro airway model exposed to ultrafine carbon black was constructed. A control group and three carbon black exposure groups (50, 100, 200 μg/ml) were set up, and the cells were treated with corresponding concentrations of ultrafine carbon black for 24 hours. In addition, the experiment was divided into control group, NAC+ control group, 100 μg/ml carbon black exposure group and NAC+ exposure group. The corresponding groups were treated with 2 mmol/L NAC for 1 h and 100 μg/ml ultrafine carbon black for 24 h, respectively. Cell viability was measured by CCK-8 assay. Intracellular reactive oxygen species (ROS) level was detected by chemical fluorescence method. The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), as well as the content of malondialdehyde (MDA) were detected by colorimetry. The mRNA and protein expressions of autophagy-related genes[Atg5, Atg7, Beclin1, microtubule-associated protein light chain 3B (LC3B), p62 and lysosome-associated membrane protein 2 (LAMP2) ] and apoptosis-related genes [B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), Caspase3, Caspase9 and poly (ADP-ribose) polymerase 1 (PARP1) ] were determined by fluorescence quantitative PCR and Western blot. Cell apoptosis was determined by flow cytometry. <b>Results:</b> Compared with the control group, the relative survival rates of BEAS-2B cells in 50, 100, 200 μg/ml carbon black exposure groups were significantly decreased, the levels of ROS and MDA were significantly increased, and the activities of SOD, GSH-Px and CAT were significantly decreased (<i>P</i><0.05). The relative survival rate, ROS and MDA levels, SOD, GSH-Px and CAT activities were significantly correlated with the exposure dose of ultrafine carbon black (<i>r</i>(s)=-0.755, 0.826, 0.934, -0.810, -0.880, -0.840, <i>P</i><0.05). Compared with the control group, the relative expression levels of Atg5, Atg7, Beclin1, LC3B, p62, LAMP2, Bax, Caspase3, Caspase9, PARP1 mRNA and Atg5, Atg7, Beclin1, LC3BⅡ, p62, LAMP2, Bax, cleaved Caspase3 (C-Caspase3), cleaved Caspase9 (C-Caspase9), cleaved PARP1 (C-PARP1) protein and the ratio of LC3BⅡ/LC3BⅠ in 50, 100 and 200 μg/ml carbon black exposure groups were significantly increased, while the relative expression levels of Bcl-2 mRNA and protein were significantly decreased (<i>P</i><0.05). The changes of the above indexes were significantly correlated with the exposure dose of carbon black (<i>r</i>(s)=0.892, 0.879, 0.944, 0.892, 0.828, 0.880, 0.814, 0.794, 0.931, 0.918, 0.813, 0.866, 0.774, 0.695, 0.918, 0.761, 0.79","PeriodicalId":23958,"journal":{"name":"中华劳动卫生职业病杂志","volume":"42 9","pages":"656-667"},"PeriodicalIF":0.0,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142476231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}