Zeitschrift fur Immunitatsforschung. Immunobiology最新文献_第8页

The significance of cytotoxicity of lymphocyte populations in coloncarcinoma patients in comparison to normal controls using a new statistical method of evaluation. 用一种新的统计方法评价大肠癌患者淋巴细胞群细胞毒性与正常对照的意义。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1977-07-01

H Warnatz, E Eder, D Lackner, G Hommel

{"title":"The significance of cytotoxicity of lymphocyte populations in coloncarcinoma patients in comparison to normal controls using a new statistical method of evaluation.","authors":"H Warnatz, E Eder, D Lackner, G Hommel","doi":"","DOIUrl":"","url":null,"abstract":"Lymphocytotoxicity was studied in patients suffering from colon carcinoma. 125Iododeoxyuridin-labeled established colon carcinoma cells were used as target cells in a cytotoxicity assay according to COHEN et al. (1). They were incubated with unfractionated lymphocytes of the peripheral blood, purified T cells or adherent cells as effector cells. The cytotoxic effect was measured in cpm, i. e. the radioactivity released from the target cells into the supernatant. The cpm were adjusted by eliminating the variables of spontaneous release and for maximal incorporated radioactivity of the target cells. The cytotoxic activity of lymphocytes to colon carcinoma target cells was dose-dependent. It was demonstrated for lymphocytes from colon carcinoma patients as well as for lymphocytes from healthy controls. Although adjusted cpm values were used for statistical analysis lymphocytes from the 20 colon carcinoma patients did not show a higher cytotoxic effect than lymphocytes from the 20 healthy controls. However, when the results were compared separately for each experiment consisting of 2 colon carcinoma patients and 3 healthy controls, statistical significant specific lymphocytotoxicity was demonstrated for lymphocytes from colon carcinoma patients. The plot diagram of the results and the statistical evaluation by the multivariate analysis of variance (Manova) offer a clear and comprehensible representation of the results. Particularly for statistical reasons, this representation is superior to the usual calculation of percent specific lysis. It was demonstrated in this study that the cytotoxic activity in the absence of autologous serum is a function of T cells of the peripheral blood. Adherent cells have a high cytotoxic activity regardless whether the cells were obtained from colon carcinoma patients or from healthy controls.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"153 2","pages":"128-42"},"PeriodicalIF":0.0,"publicationDate":"1977-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12089691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An immunoperoxidase method for the identification of B lymphocytes by light microscopy. 免疫过氧化物酶法在光学显微镜下鉴定B淋巴细胞。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1977-07-01

C Kaschka-Dierich, G Bandlow, R Thomssen

引用次数: 0

In vitro and in vivo immune response to specific antigens in canine marrow graft recipients. 犬骨髓移植受者对特异性抗原的体内外免疫反应。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1977-07-01

J Abb, H J Kolb, H V Rodt, H Grosse-Wilde, I Rieder, S Thierfelder

{"title":"In vitro and in vivo immune response to specific antigens in canine marrow graft recipients.","authors":"J Abb, H J Kolb, H V Rodt, H Grosse-Wilde, I Rieder, S Thierfelder","doi":"","DOIUrl":"","url":null,"abstract":"Cellular and humoral immune reactivity to primary and secondary challenge with tetanus toxoid, diphtheria toxoid and keyhole limpet hemocyanin was studied in normal dogs and canine bone marrow chimeras prepared for marrow grafting by lethal doses of cyclophosphamide. Short-term chimeras (less than 100 days postgrafting) showed general impairment of immune function as indicated by marked depression of skin test reactivity, lymphocyte blastogenesis in vitro and antibody formation. Long-term chimeras (more than 100 days post-grafting) demonstrated gradual improvement of immunologic capacity related to the stage of postgrafting. Prolonged immunoincompetence was suggested by decreased lymphocyte proliferation in vitro in response to stimulation with tetanus and diphtheria toxoids and by incomplete conversion from 19S to 7S antibody synthesis. Lymphocyte blastogenesis to specific antigens may prove a useful parameter for the in vitro evaluation of defects in cell-mediated immunity following allogeneic bone marrow transplantation. Our findings of prolonged immunodeficiency in the preclinical canine model point out the necessity for vigilance in early detection and treatment of infection and underline the importance of approaches aimed at accelerating immunologic reconstitution.","PeriodicalId":23935,"journal":{"name":"Zeitschrift fur Immunitatsforschung. Immunobiology","volume":"153 2","pages":"152-61"},"PeriodicalIF":0.0,"publicationDate":"1977-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11292778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple technique for measuring leucocyte chemotaxis in reversible Boyden chambers. 可逆博伊登腔中白细胞趋化性测定的简单技术。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1977-07-01

H Arvilommi, A Laatikainen

引用次数: 0

A simple technique for measuring leucocyte chemotaxis in reversible Boyden chambers. 可逆博伊登腔中白细胞趋化性测定的简单技术。

Zeitschrift fur Immunitatsforschung. Immunobiology Pub Date : 1977-07-01 DOI: 10.1016/S0340-904X(77)80051-1

H. Arvilommi, A. Laatikainen

引用次数: 3

A micro-method for PHA-induced stimulation of human lymphocytes. II. communication: effect of splenectomy on the inhibitory activity of serum in patients with Hodgkin's disease. pha诱导人淋巴细胞刺激的微方法。2通讯:脾切除术对霍奇金病患者血清抑制活性的影响。