H Fuhrmann, S Schultheis, W Drommer, F J Kaup, H P Sallmann
{"title":"[Tissue lipid peroxidation in nutritional encephalomalacia of broiler chickens].","authors":"H Fuhrmann, S Schultheis, W Drommer, F J Kaup, H P Sallmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The consequences of different dietary fats in combination with two vitamin E levels on peroxidative tissue damage of chicken brain and liver and its meaning for development of nutritional encephalomalacia (NE) were investigated. A feeding experiment was performed with 1-day-old chickens from hens on a vitamin-E-poor diet. The animals received a vitamin-E-deficient basic diet containing 10% fat, rich in either C18:3n3-, C18:2n6- or C18:1n9-fatty acids. The fat was given either fresh or oxidized (peroxidation number: 250) and 0 or 50 ppm alpha-tocopherylacetate was added. Typical symptoms of NE occurred mainly in those groups fed with n6-fatty acids beginning on day 7. In order to evaluate oxidative tissue damage, conjugated dienes, fluorescent pigments and TBA-reactive substances were determined in liver, cerebrum and cerebellum. Brain was examined histologically. In liver and cerebrum, the feeding of oxidized fats led to a 20% increase in conjugated dienes. Fluorescent pigments could be determined only in the brain tissues. However, feeding conditions had no effect, although autofluorescence was observed histologically in the affected animals. TBA-reactive substances were heightened in cerebrum (30%) and liver (130%) as a result of feeding linolenic acid. Vitamin E deficiency doubled TBA-reactive substances only in the liver. The parameters measured did not show intensified lipid peroxidation in the cerebellum of the animals fed the NE producing diet. Rather, the liver seems to be affected by the oxidative stress.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"43 1","pages":"9-21"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19667908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Measurements of the activity of the coagulation factors VIII:C, IX,XI, and XII in cats].","authors":"R Mischke, A Deniz, I Nolte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using an optimized test, reference ranges were established for the activity of the coagulation factors VIII:C (72-124%), IX (81-130%), XI (69-134%) and XII (51-142%) in the cat (n = 58 cats, 2.5-97.5% quantile). Compared to a reference curve prepared by using a cat-pool plasma (n = 50), the factor VIII:C activity in humans (commercial human reference plasma) was 7.6%, i.e. the factor VIII:C activity in cats was 13 fold higher than in humans. The values for the factors IX, XI and XII depended distinctly on the dilution step of the human plasma. The activity of the cat in relation to humans was approximately 90% (factor IX), 170% (factor XI) and 135% (factor XII). Two clinically healthy cats who were conspicuous pre-operative because of a distinctly prolonged, activated partial thromboplastin time showed a decreased factor XII activity.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 8","pages":"513-20"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19572377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Changes in calcium concentration in bovine udder secretions during the preparatory stages of labor in relation to prediction of the time of calving].","authors":"E Grunert, E H Birgel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nine heifers and 10 cows of the breed 'German Black Pied' were used in order to evaluate the practicability of measurement of changes in concentration of calcium in udder secretions during the preparatory stage of labour for the prediction of the time of calving. A gradual increase in the concentration of calcium in udder secretions ante partum (a.p.) was determined. Eleven days a.p., the average concentration of calcium was 14.2 mmol/l. Thereafter, the calcium content gradually increased and reached a mean value of 52.1 mmol/l sub partu (secretions of all udder quarters), and 52.4 mmol/l and 51.8 mmol/l in secretions of fore and hind quarters, respectively. Despite the increase in calcium, the time of calving in individual animals could not be predicted reliably, or with a high probability, because the increase in calcium content did not occur in all cows, the variation of the values was high, and the differences between values during the last 48 h a.p. were only small.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 7","pages":"479-84"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19574767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Description and determination of the diameter of arteries and veins in the hindlimb of cattle using B-mode ultrasonography].","authors":"J Kofler","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The large arteries and veins of both metatarsal and digital regions of 14 healthy, non-pregnant cows with a mean age of 6.1 +/- 1.7 years were examined sonographically (real-time B-mode system with a 7.5 MHz linear-transducer). The ultrasonographic imaging of the larger vessels was investigated, diameters were measured, especially of the digital veins, in which intravenous regional anaesthesia and antibiosis is usually applied. Three measurements per cow on each distal hindlimb were noted over a period of 3 weeks using defined anatomical locations. The mean diameter of the vessels were as follows: Arteria (A.) dorsalis pedis 4.1 +/- 0.4 mm, Vena (V.) dorsalis pedis 5.2 +/- 0.5 mm, A. metatarsea dorsalis III 3.3 +/- 0.4 mm, A. digitalis dorsalis communis III 3.9 +/- 0.4 mm, V. digitalis dorsalis communis III 2.7 +/- 0.4 mm, V. digitalis plantaris communis IV 2.1 +/- 0.4 mm, V. digitalis plantaris communis II 2.1 +/- 0.3 mm, V. metatarsea plantaris lateralis 4.5 +/- 0.8 mm, V. metatarsea plantaris medialis 4.4 +/- 0.6 mm. The Ramus cranialis of the V. saphena lateralis, the A. and V. digitalis plantaris communis III and the A. digitalis plantaris communis II and IV could only be imaged in a few cases. Except for V. digitalis dorsalis communis III, statistical analysis of the mean diameters of the examined vessels of the right and the left distal hindlimb in 14 cows revealed no significant differences (P < 0.05). B-mode ultrasonography proved a suitable method for imaging and examination of the vessels larger than 1 mm in the distal bovine hindlimb and can be recommended as the non-invasive method of choice for the examination of blood vessels in cattle.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 4","pages":"253-66"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18503504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Diagnostic and pathophysiological aspects of the determination of kidney function in animals].","authors":"S Reder, H Hartmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Early diagnosis of loss in renal function (< 60-70%) is not possible either by resorting to the parameters of plasma urea and creatinine concentrations (responsive to functional loss by > 75% or by reference to urine concentration capacity (urine density: sensitive to concentrations > 60%). However, clearance techniques for determination of the glomerular filtration rate (GFR) have proved suitable for quantitative assessment of renal function. Endogenous creatinine clearance is one of the most common clinical approaches in GFR determination. Criticism of results obtainable from endogenous creatinine clearance appears to be justified by pharmacokinetic aspects of creatinine as an indicator, as well as by some of its analytical peculiarities. The tediousness of the procedure is another counterproductive aspect pertaining to large-scale use of endogenous creatinine clearance in veterinary medicine. Total blood-plasma clearance of exogenous creatinine (T-Clexo.Creatinine) would provide vets with an accurate (diagnostic validity) and practical method for carrying out clinical kidney-function diagnostics. However, more research on a number of related issues will be required before the general introduction of the procedure.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 3","pages":"253-67"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19558562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Evaluation of hypoxic conditions in calves using the erythrocyte density test (EDT)].","authors":"T Flegel, H Hartmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The erythrocyte density separation test (EDT) divides the red blood cells into two groups: younger (less dense) and older (more dense) erythrocytes. Using this test enables veterinarians to assess the erythropoiesis in calves on the basis of the percentage of less dense (younger) red blood cells. Anemic calves, as well as those with pneumonic infections show higher proportions of less dense red blood cells in the EDT than healthy ones. This testing procedure makes it possible to estimate the effects of an oxygen deficiency condition, such as hypoxemia, anemia, shock etc. on the peripheral tissues. So the EDT represents a valuable complement to existing hematological laboratory methods. Carrying out the EDT is very simple and suitable in routine clinical testing.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 3","pages":"215-28"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19558616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The influence of semen preparation and culture medium on the success of IVF in cattle].","authors":"C Rosenkranz, A Holzmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of two semen-preparation methods and the presence and absence of capacitation-inducing supplements in the fertilization medium on IVF-results were examined in a 2 x 2 factorial design. In vitro matured oocytes were fertilized either with transmigrated (TM) or with swim-up (SU) prepared native semen. In the first group, the fertilization medium contained 0.8 microgram/ml heparin, 1.2 micrograms/ml hypotaurine and 0.2 microgram/ml epinephrine. However, in the second group, no capacitation-inducing or motility-enhancing substances were used. After SU-treatment, the supplements were necessary to obtain sufficient fertilization results. In the second group (non-supplemented medium), the percentages of penetrated and fertilized oocytes were decreased significantly (P < 0.001). By contrast, transmigrated semen required no additional supplementation of the fertilization medium. The penetration and fertilization rates of this semen-preparation method were equal in both groups.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 2","pages":"139-43"},"PeriodicalIF":0.0,"publicationDate":"1995-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19572433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The effect of a different energy supply for growing Great Danes on the body mass and skeletal development. 3. Clinical picture and chemical studies of the skeleton].","authors":"J Zentek, H Meyer, K Dämmrich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In growth studies with young Great Danes (0-6 months old), the effects of ad libitum (nine animals) or restricted feed supply (70-80% of ad libitum supply; 17 dogs, 9 of them bearing additional weight loads, 15% of body weight attached to the shoulder) were investigated with regard to development of the skeleton and bone composition. Growth disturbances of the skeleton were seen in every group, but the incidence increased with ad libitum feeding. Fast growth led to the valgus syndrome in fore- and hindlimbs, while hyperextensions of the carpal joint were seen, independently of the growth intensity. Weight loading in connection with energy restriction could not reproduce the same effects as ad libitum feeding. The mineral and protein concentrations in the bones (related to fat-free dry matter) were not affected either by the different feeding levels or by the weight loading.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"42 1","pages":"69-80"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19573091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Generalized ceroid lipofuscinosis in cocker spaniels].","authors":"A Kirchhoff, C Kobe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Results of macroscopic, microscopic and electronmicroscopic investigations of two cocker spaniels with generalized ceroid-lipofuscinosis are recorded. In the nerve system, the lipofuscin granules were mainly localized, primarily in nervous cells and secondarily in glial cells of the medulla oblongata and spinal cord. Extraneuronal lipofuscin granules were found in the smooth muscle fibres (bowel, bladder, trabeculae of the spleen, prostata etc.) and also in the cells of the exocrine pancreas. Electronmicroscopically the lipofuscin granules in the neurons and in the smooth muscle fibres showed the 'typical' structure of lipofuscin, whereas in macrophages and glial cells the lipofuscin appeared as fingerprint-like, pleomorphic, curvilinear and multi-membranous bodies.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"41 10","pages":"731-40"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18658053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Measuring fibrinogen concentrations in healthy dogs: standardization, comparison of methods and reference values].","authors":"R Mischke, D Menzel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Plasma fibrinogen concentration was measured in 67 healthy, adult dogs using five different methods (gravimetry, methods described by JACOBSSON (1955) RATNOFF and MENZIE (1951), and CLAUSS (1957), and functional photometric assay). Apart from using linear regression and the Pearson correlation coefficient (r) in order to characterize the relation between different methods, reference ranges (2.5-97.5%-fractile) were calculated for all methods. For calibration of the CLAUSS method (1957) and the photometric assay, dog plasma with a defined fibrinogen concentration was used. Measurements of commercial human fibrinogen standards yielded a good conformity with the concentrations specified by the manufacturer (values approximately 3% too low). These standards appear, therefore, to be also suited to the calibration of measurements of dog fibrinogen. The reference range for the gravimetry was 1.08-2.88 g fibrinogen per litre of plasma. A considerable conformity and close correlation was seen between the fibrinogen concentration measured by gravimetry and by using methods described by JACOBSSON (1955; y = 1.088 x -0.142, r = 0.967) or CLAUSS (1957; y = 0.999 x -0.004, r = 0.973), respectively. Between the reference-method gravimetry and the photometric method and RATNOFF-MENZIE (1951) method, respectively, a less close correlation, as well as a minor conformity, was found.</p>","PeriodicalId":23904,"journal":{"name":"Zentralblatt fur Veterinarmedizin. Reihe A","volume":"41 8","pages":"587-98"},"PeriodicalIF":0.0,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18734657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}