Zebrafish最新文献_第9页

First Record of Clinostomum sp. (Digenea: Clinostomidae) in Danio rerio (Actinopterygii: Cyprinidae) and the Implication of Using Zebrafish from Pet Stores on Research. Danio rerio(放线鱼科:鲤科)首次记录Clinostomum sp. (Digenea: Clinostomidae)及利用宠物店斑马鱼进行研究的意义。

IF 2 4区生物学

Zebrafish Pub Date : 2021-04-01 Epub Date: 2021-03-02 DOI: 10.1089/zeb.2020.1950

Tony Silveira, Mateus T Kütter, Camila M G Martins, Luis Fernando Marins, Robert T Boyle, Vinicius F Campos, Mariana H Remião

{"title":"First Record of Clinostomum sp. (Digenea: Clinostomidae) in Danio rerio (Actinopterygii: Cyprinidae) and the Implication of Using Zebrafish from Pet Stores on Research.","authors":"Tony Silveira, Mateus T Kütter, Camila M G Martins, Luis Fernando Marins, Robert T Boyle, Vinicius F Campos, Mariana H Remião","doi":"10.1089/zeb.2020.1950","DOIUrl":"https://doi.org/10.1089/zeb.2020.1950","url":null,"abstract":"Many scientific studies still use zebrafish from pet stores as animal models, even cutting-edge researches. However, these animals differ genotypically and phenotypically between them. The importance of the use of standardized models is widely recognized. Besides that, another consequence of using zebrafish from unknown origins is the acquisition of parasitized animals. This study aimed to relate the infection by Clinostomum sp. in zebrafish. Animals sold as \"high standard\" were acquired from a commercial company. Swimming alterations and superficial yellow dots were observed in five zebrafish with clinical signs, which were isolated, euthanized, and necropsied. Muscular yellow cysts with metacercaria associated with lesions were observed. The muscular cysts were responsible for the superficial yellow dots as well as the swimming alterations. The prevalence was 2.5%, and the mean infection intensity was 7 digeneans/host. The cysts measured a mean of 1251.43 μm long × 784.28 μm wide. Metacercariae measured a mean of 4847 μm long × 1353 μm wide. This first report about infection by Clinostomum sp. in zebrafish is globally relevant since the host and the parasite genus currently overlap worldwide. Furthermore, this study sheds light on the importance of the specific pathogen-free commercial creations or laboratory-reared zebrafish for research.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 2","pages":"139-148"},"PeriodicalIF":2.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25424262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Long-Term Exposure of Alcohol Induced Behavioral Impairments and Oxidative Stress in the Brain Mitochondria and Synaptosomes of Adult Zebrafish. 酒精对成年斑马鱼脑线粒体和突触体行为损伤和氧化应激的长期影响。

IF 2 4区生物学

Zebrafish Pub Date : 2021-04-01 Epub Date: 2021-03-15 DOI: 10.1089/zeb.2020.1913

Manisha Nahar, Deepali Jat

{"title":"Long-Term Exposure of Alcohol Induced Behavioral Impairments and Oxidative Stress in the Brain Mitochondria and Synaptosomes of Adult Zebrafish.","authors":"Manisha Nahar, Deepali Jat","doi":"10.1089/zeb.2020.1913","DOIUrl":"https://doi.org/10.1089/zeb.2020.1913","url":null,"abstract":"Alcoholism causes deleterious effects such as physiological and neuronal alterations leading to the cognitive and other behavioral impairments. Mitochondrial and synaptosomal deteriorations in the brain of alcoholic persons exhibited metabolic, biochemical changes and other related risk factors, which mainly affect the brain function. This study aimed to assess the effect of chronic alcohol-induced mitochondrial and synaptosomal oxidative damage along with behavioral impairment in adult zebrafish. Zebrafish of control group received the system water and normal diet ad libitum (group I); the other groups were treated with 0.20% alcohol (group II) and 0.40% alcohol (group III) directly in fish tank for 22 days. The result revealed significant increase in lipid peroxidation, protein carbonylation, superoxide dismutase, and glutathione, and significant decline in the activity of catalase and Na+/K+ ATPase compared to control. Furthermore, the alcohol-treated zebrafish also showed significant behavioral alterations. Collectively, this regulatory mechanism demonstrates the effect of long-term alcohol consumption in the zebrafish. Our results indicate that this recreational drug \"alcohol\" is harmful to brain mitochondria and synaptosomes, which are the main organelles, and play an important role in memory, learning, cognitive function, and ATP formation in the brain, which may represent a significant public health concern.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 2","pages":"110-124"},"PeriodicalIF":2.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25486467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hidden Evolutionary Units and Its Implications on Conservation in a Vulnerable Species of a Freshwater Fish. 一个淡水鱼脆弱物种的隐藏进化单位及其保护意义。

IF 2 4区生物学

Zebrafish Pub Date : 2021-04-01 Epub Date: 2021-02-24 DOI: 10.1089/zeb.2020.1916

Snaydia Viegas Resende, Iuri Batista Silva, Rubens Pasa, Alexandre Wagner Silva Hilsdorf, Karine Frehner Kavalco

{"title":"Hidden Evolutionary Units and Its Implications on Conservation in a Vulnerable Species of a Freshwater Fish.","authors":"Snaydia Viegas Resende, Iuri Batista Silva, Rubens Pasa, Alexandre Wagner Silva Hilsdorf, Karine Frehner Kavalco","doi":"10.1089/zeb.2020.1916","DOIUrl":"https://doi.org/10.1089/zeb.2020.1916","url":null,"abstract":"Brycon is a fish genus in the order Characiformes, distributed from southern Mexico to the La Plata River in Argentina. Several of its species, including Brycon nattereri, are threatened with extinction or considered vulnerable because they are highly sensitive to anthropogenic factors. The decline of these species may be related to the growth of agriculture and mining in the Brazilian Cerrado region, thus their recovery requires management plans. In this study, we use morphological, chromosomal, and genetic analysis to suggest that two distinct evolutionary units exist under the same denomination B. nattereri, indistinguishable by the descriptive morphological characters of the species until the present moment and show that the population of the São Francisco River is more diverse than that of the upper Paraná River basin. These results may help with future management and conservation programs of Brycon species in the Paraná and São Francisco river basins, two major Brazilian hydrographic basins.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 2","pages":"149-161"},"PeriodicalIF":2.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25399419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TAEL 2.0: An Improved Optogenetic Expression System for Zebrafish. 改进的斑马鱼光遗传表达系统TAEL 2.0。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-02-08 DOI: 10.1089/zeb.2020.1951

Jesselynn LaBelle, Adela Ramos-Martinez, Kyle Shen, Laura B Motta-Mena, Kevin H Gardner, Stefan C Materna, Stephanie Woo

{"title":"TAEL 2.0: An Improved Optogenetic Expression System for Zebrafish.","authors":"Jesselynn LaBelle, Adela Ramos-Martinez, Kyle Shen, Laura B Motta-Mena, Kevin H Gardner, Stefan C Materna, Stephanie Woo","doi":"10.1089/zeb.2020.1951","DOIUrl":"https://doi.org/10.1089/zeb.2020.1951","url":null,"abstract":"Inducible gene expression systems are valuable tools for studying biological processes. We previously developed an optogenetic gene expression system called TAEL that is optimized for use in zebrafish. When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter. By using light as the inducing agent, the TAEL/C120 system overcomes limitations of traditional inducible expression systems by enabling fine spatial and temporal regulation of gene expression. In this study, we describe ongoing efforts to improve the TAEL/C120 system. We made modifications to both the TAEL transcriptional activator and the C120 regulatory element, collectively referred to as TAEL 2.0. We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system. With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter. We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system. This improved optogenetic expression system will be a broadly useful resource for the zebrafish community.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 1","pages":"20-28"},"PeriodicalIF":2.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020536/pdf/zeb.2020.1951.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25344468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Last Half Century of Fish Explant and Organ Culture. 近半个世纪的鱼类外植体和器官培养。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-01-18 DOI: 10.1089/zeb.2020.1935

Elizabeth E LeClair

引用次数: 2

The ZE-Tunnel: An Affordable, Easy-to-Assemble, and User-Friendly Benchtop Zebrafish Swim Tunnel. 隧道:一个负担得起的，易于组装，和用户友好的台式斑马鱼游泳隧道。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-01-12 DOI: 10.1089/zeb.2020.1948

Jan Willem Bek, Adelbert De Clercq, Paul J Coucke, Andy Willaert

{"title":"The ZE-Tunnel: An Affordable, Easy-to-Assemble, and User-Friendly Benchtop Zebrafish Swim Tunnel.","authors":"Jan Willem Bek, Adelbert De Clercq, Paul J Coucke, Andy Willaert","doi":"10.1089/zeb.2020.1948","DOIUrl":"https://doi.org/10.1089/zeb.2020.1948","url":null,"abstract":"The popularity of zebrafish in both basic biological and biomedical research has led to an increased need for understanding their behavior. Locomotor behavior is an important outcome of different factors, such as specific genotypes or external stimuli that influence the nervous and musculoskeletal system. Locomotion can be studied by forced swimming in a swim tunnel, a device capable of generating a laminar water flow at different speeds in a chamber where zebrafish can be placed. However, commercially available swim tunnels are relatively expensive and in-house built systems are mostly presented without clear building instructions or proper validation procedures. In this study, we developed an alternative, cheap (<250 euro), and user-friendly, but customizable benchtop swim tunnel, called the \"Zebrafish exercise-tunnel\" (ZE-Tunnel). Detailed step-by-step instructions on how to construct the tunnel components, including the frame, mechanical, and electric components are given. The ZE-Tunnel was reliably used to exercise fish for prolonged periods and its performance was successfully validated by replicating previously published experiments on critical speed testing in zebrafish. Finally, implementation of behavioral video analysis using freely available motion-tracking software showed differences in swimming dynamics in the Chihuahua skeletal zebrafish mutant.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 1","pages":"29-41"},"PeriodicalIF":2.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38806036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Perturbations in Amino Acid Metabolism in Reserpine-Treated Zebrafish Brain Detected by ¹H Nuclear Magnetic Resonance-Based Metabolomics. 利用基于1H核磁共振的代谢组学检测利血平处理的斑马鱼大脑中氨基酸代谢的扰动。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-02-03 DOI: 10.1089/zeb.2020.1895

Fauziahanim Zakaria, Muhammad Tayyab Akhtar, Wan Norhamidah Wan Ibrahim, Noraini Abu Bakar, Azira Muhamad, Shamarina Shohaimi, Maulidiani Maulidiani, Hafandi Ahmad, Intan Safinar Ismail, Khozirah Shaari

{"title":"Perturbations in Amino Acid Metabolism in Reserpine-Treated Zebrafish Brain Detected by 1H Nuclear Magnetic Resonance-Based Metabolomics.","authors":"Fauziahanim Zakaria, Muhammad Tayyab Akhtar, Wan Norhamidah Wan Ibrahim, Noraini Abu Bakar, Azira Muhamad, Shamarina Shohaimi, Maulidiani Maulidiani, Hafandi Ahmad, Intan Safinar Ismail, Khozirah Shaari","doi":"10.1089/zeb.2020.1895","DOIUrl":"https://doi.org/10.1089/zeb.2020.1895","url":null,"abstract":"Depression is a complex and disabling psychiatric disorder, which is expected to be a leading cause for disability by 2030. According to World Health Organization, about 350 million people are suffering with mental health disorders around the globe, especially depression. However, the mechanisms involved in stress-induced depression have not been fully elucidated. In this study, a stress-like state was pharmacologically induced in zebrafish using reserpine, a drug widely used to mediate depression in experimental animal models. Zebrafish received single intraperitoneal (i.p.) injections of 20, 40, and 80 mg/kg body weight reserpine doses and were subjected to open-field test at 2, 24, 48, 72, and 96 h after the treatment. Along with observed changes in behavior and measurement of cortisol levels, the fish were further examined for perturbations in their brain metabolites by 1H nuclear magnetic resonance (NMR)-based metabolomics. We found a significant increase in freezing duration, whereas total distance travelled was decreased 24 h after single intraperitoneal injection of reserpine. Cortisol level was also found to be higher after 48 h of reserpine treatment. The 1H NMR data showed that the levels of metabolites such as glutamate, glutamine, histamine, valine, leucine and histidine, lactate, l-fucose, betaine and γ-amino butyric acid (GABA), β-hydroxyisovalerate, and glutathione were significantly decreased in the reserpine-treated group. This study provided some insights into the molecular nature of stress that could contribute toward a better understanding of depression disorder.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 1","pages":"42-54"},"PeriodicalIF":2.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25329791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Peptide Profile of Zebrafish Brain in a 6-OHDA-Induced Parkinson Model. 6-羟多巴胺诱导的帕金森模型斑马鱼脑肽谱

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-02-09 DOI: 10.1089/zeb.2020.1945

Louise Oliveira Fiametti, Claudia Neves Correa, Leandro Mantovani de Castro

引用次数: 6

Chromosomal Diversity in Two Allopatric Populations of Farlowella hahni Meinken 1937 (Teleostei: Siluriformes): Cytogenetics and Cytochrome b Analyses. 两个异域居群1937 (Teleostei: Siluriformes)的染色体多样性:细胞遗传学和细胞色素b分析。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-02-03 DOI: 10.1089/zeb.2020.1966

Carlos Alexandre Fernandes, Leonardo Marcel Paiz, Diovani Piscor, Mariane Gavazzoni, Luciana Andreia Borin de Carvalho, Ana Luiza de Brito Portela-Castro, Vladimir Pavan Margarido

{"title":"Chromosomal Diversity in Two Allopatric Populations of Farlowella hahni Meinken 1937 (Teleostei: Siluriformes): Cytogenetics and Cytochrome b Analyses.","authors":"Carlos Alexandre Fernandes, Leonardo Marcel Paiz, Diovani Piscor, Mariane Gavazzoni, Luciana Andreia Borin de Carvalho, Ana Luiza de Brito Portela-Castro, Vladimir Pavan Margarido","doi":"10.1089/zeb.2020.1966","DOIUrl":"https://doi.org/10.1089/zeb.2020.1966","url":null,"abstract":"Farlowella is the second richest genus in Loricariinae, broadly distributed in freshwater streams and rivers of South America. In this article, we aimed to expand on the cytogenetic and molecular data available for two allopatric populations of Farlowella hahni. Both populations had diploid chromosome number 58, but with karyotype differences, indicative of chromosomal rearrangements. C-banding showed large heterochromatic blocks at telomeric regions in acrocentric chromosomes in both populations. Fluorescence in situ hybridization (FISH) revealed a single 18S rDNA site in both populations and a single 5S rDNA site for individuals from lower Paraná River basin (native region) and multiple 5S rDNA sites for individuals from upper Paraná River basin (non-native region). Mitochondrial sequence analyses did not separate the two F. hahni populations. The cytogenetic and molecular data obtained are relevant in a preliminary study and suggested the existence of cryptic diversity and the hypothesis that at least two Farlowella lineages may coexist in the Paraná basin.","PeriodicalId":23872,"journal":{"name":"Zebrafish","volume":"18 1","pages":"66-72"},"PeriodicalIF":2.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25330194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol. 斑马鱼胚胎基因型方案影响的细胞和分子表征。

IF 2 4区生物学

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-01-21 DOI: 10.1089/zeb.2020.1958

Alon M Douek, Emma I Klein, Jan Kaslin, Peter D Currie, Avnika A Ruparelia

引用次数: 1