斑马鱼胚胎基因型方案影响的细胞和分子表征。

IF 1.4 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Zebrafish Pub Date : 2021-02-01 Epub Date: 2021-01-21 DOI:10.1089/zeb.2020.1958

Alon M Douek, Emma I Klein, Jan Kaslin, Peter D Currie, Avnika A Ruparelia

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引用次数: 1

摘要

斑马鱼胚胎基因分型(ZEG)装置为活胚胎基因分型提供了一种很有前途的工具。虽然使用ZEG后胚胎的大体形态和存活不受影响，但ZEG方案的细胞和分子效应仍然未知。为了解决这个问题，我们检查了特定组织的完整性，并评估了应激反应基因的表达，以确定ZEG方案的影响。我们的分析表明，虽然ZEG导致低水平的急性应激反应，但没有明显的长期影响，支持其用于各种下游分析。

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Cellular and Molecular Characterization of the Effects of the Zebrafish Embryo Genotyper Protocol.

The Zebrafish Embryo Genotyper (ZEG) device provides a promising tool for genotyping live embryos. Although the gross morphology and survival of embryos after the use of ZEG are unaffected, the cellular and molecular effects of the ZEG protocol remain unknown. To address this, we have examined the integrity of specific tissues, and evaluated the expression of stress-responsive genes to determine the impact of the ZEG protocol. Our analyses reveal that although ZEG results in a low-level acute stress response, no long-lasting effects are evident, supporting its utilization for a variety of downstream assays.

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来源期刊

Zebrafish DEVELOPMENTAL BIOLOGY-ZOOLOGY

CiteScore

3.60

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： Zebrafish is the only peer-reviewed journal dedicated to the central role of zebrafish and other aquarium species as models for the study of vertebrate development, evolution, toxicology, and human disease. Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is a prime model for genetic and developmental studies. While genetically more distant from humans, the vertebrate zebrafish nevertheless has comparable organs and tissues, such as heart, kidney, pancreas, bones, and cartilage. Zebrafish introduced the new section TechnoFish, which highlights these innovations for the general zebrafish community. TechnoFish features two types of articles: TechnoFish Previews: Important, generally useful technical advances or valuable transgenic lines TechnoFish Methods: Brief descriptions of new methods, reagents, or transgenic lines that will be of widespread use in the zebrafish community Zebrafish coverage includes: Comparative genomics and evolution Molecular/cellular mechanisms of cell growth Genetic analysis of embryogenesis and disease Toxicological and infectious disease models Models for neurological disorders and aging New methods, tools, and experimental approaches Zebrafish also includes research with other aquarium species such as medaka, Fugu, and Xiphophorus.