{"title":"[Study of amino acid and carbohydrate metabolism of porcine strains of Haemophilus by thin layer chromatography].","authors":"E Balke, R Weiss, A Seipp","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of the present study was to investigate porcine reference and field strains of the species Haemophilus (H.) pleuropneumoniae and H. parasuis, as well as H. Taxon \"minor group\" and Taxon C on their amino acid and carbohydrate metabolism by thin layer chromatography. The 17 reference strains studied showed almost identical results within the different species and taxa in both, amino acid and carbohydrate metabolism patterns. Based on a few differing enzymatic reactions a reduced species- and taxon-specific reaction pattern could be established, which included L-alanine, L-citrulline and L-threonine of the amino acids as well as D-ribose, alpha-D-xylose, mannitol, trehalose, beta-melibiose and alpha-lactose of the carbohydrates. This differentiation system allowed a reliable identification of 7 field strains whereas 4 additional ones, hitherto pre-classified as H. parasuis, could not be associated with any of the above species and taxa.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 4","pages":"495-505"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14507721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Haemolytic activity and characteristics of plasmid and chromosomally borne hly genes isolated from E. coli of different origin.","authors":"H M Grünig, G Lebek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>20 hly plasmids of different origin and source and 8 recombinant plasmids containing either chromosomally or plasmid encoded hly determinants were characterized with emphasis on their haemolytic activity. Remarkable differences among the hly genes tested were found in their haemolytic activity and in the regulation of the haemolytic activity by the extracellular iron concentration. Under iron-limiting growth conditions the haemolysin secretion encoded by 6 hly plasmids, but not by the remaining 14 hly plasmids and the chromosomal hly genes, was significantly induced. The 6 plasmids all derived from human isolates, belonged to the incompatibility groups FIV and FVI and showed similar restriction patterns. Two hly determinants, encoding an inducible haemolysin secretion, cloned from plasmids isolated in Berne and Paris, respectively, showed identical restriction maps, indicating a common origin. Compared to the hly determinant of a plasmid encoding a noninducible haemolysin secretion, distinct differences, mainly in the flanking regions, were observed.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 4","pages":"485-94"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13975657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[A natural focus of early summer meningoencephalitis virus in Tyrol].","authors":"A Radda","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Some hospitalized cases of Tick-borne encephalitis (TBE) were observed in the area of Zell am Ziller in Tyrol. Therefore, we collected ticks from Zellberg near Zell am Ziller in order to demonstrate the occurrence of a focus of TBE virus by isolation experiments with these ticks. Two virus strains could be isolated. A geoecological and phytosociological characterisation of this focus is presented in this paper.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 4","pages":"570-5"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13975661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Prevention of lymphatic and hematologic spreading as a pathogenic condition for neural persistence of herpes simplex virus].","authors":"A Gerritzen, K E Schneweis","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the experimental model of genital herpes simplex virus (HSV) infection of the mouse--in spite of abundant virus replication on the mucous membranes--no infectious virus can be isolated from the inflamed and swollen draining lymph nodes (DLN), contrary to the positive results in the lumbosacral nerves and their associated ganglia. Attempts to reactivate an abortive infection possibly established in lymph node or spleen cells by stimulation with phytohemagglutinin or lipopolysaccharide rendered no positive results, not even when adult (i.e. at least 6-week old), but immunodeficient mice were used as test animals. On the other hand, isolation of infectious virus from lymph node and--at a lesser rate--from spleen cells was successful in immature 4 to 6-week old mice, particularly when these had undergone pretreatment with cyclophosphamide, silica, antimacrophage serum and/or cortisone; 5 days post infectionem being the date of optimum virus yield. HSV-1 infected mice were more frequently positive than those with HSV-2, and genetically sensitive animals more so than resistant mice. The data indicate that the lymphohaematogenous spread of the virus is inhibited by means of an active defence mechanism, and that unspecific defence factors on the cellular level, probably macrophages and NK-cells, are essentially responsible. This reveals that the lethal generalized infection is prevented and the neural spread can gain its essential role in the pathogenesis of the HSV-infection.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 4","pages":"559-69"},"PeriodicalIF":0.0,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13975660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Morphological changes in human embryonic lung fibroblasts caused by cytotoxins of various Clostridium species].","authors":"G Schallehn, M H Wolff","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 243 strains of 35 Clostridium species were tested for cytotoxin production in cooked meat medium or liver broth within 48-72 h at 37 degrees C, using human embryonal lung fibroblasts in tissue-culture as indicator cells. Cytotoxin could be detected in the culture-filtrates of all toxigenic strains of C. chauvoei, C. difficile, C. histolyticum, C. novyi types A and B, C. septicum and C. tetani, but not in the atoxigenic ones. The cytotoxin of C. novyi correlated with alpha-toxin in the culture filtrate. All strains of C. perfringens and C. novyi D tested were not cytotoxic for lung fibroblasts despite their pathogenicity for guinea-pigs. Further cytotoxigenic strains were found among C. hastiforme, C. limosum, C. oceanicum, C. putrificum, C. ramosum, C. sordellii, C. sporogenes, and C. subterminale. The morphological changes in lung fibroblasts caused by the culture filtrates were characteristic and species-specific and corresponded with pathogenicity for guinea-pigs and/or mice. No cytotoxin was produced by C. absonum, C. barati, C. bifermentans, C. botulinum (atoxic), C. butyricum, C. cadaveris, C. carnis, C. clostridioforme, C. cochlearium, C. glycolicum, C. innocuum, C. malenominatum, C. mangenotii, C. paraputrificum, C. putrefaciens, C. rectum, C. tertium, and C. tyrobutyricum.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 3","pages":"367-78"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14503329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"International Symposium on In Vitro Cultivation of Parasites and Alternatives to Animal Experimentation in Parasitology. Zürich, Switzerland, April 9-11, 1987. Abstracts.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 2","pages":"277-305"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14572268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Salmonella daarle (6,8:y:e,n,x)--report on the isolation of a new serovar].","authors":"H P Rohr, S Aleksic","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The serological and biochemical characteristics of a so far unknown serovar of Salmonella subspecies I with the serological formula 6,8:y:e,n,x (detailed antigenic formula = 6(1),8:y:e,n,x,z16, No. IP 5526/86 = H 428-36/86) is described. It has been named after a borough of Saarbrücken: Salmonella daarle.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 2","pages":"186-7"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14572263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epidemiology (1980-1985) and nonspecific prophylaxis of Q fever in the USSR (survey).","authors":"I V Tarasevich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A review of the epidemiology and nonspecific prophylaxis of Q fever in the USSR during 1980-1985 is presented. Attention is paid of the source to infection, the seroprevalence of antibodies to Coxiella burnetii in man and livestock and the possibilities of prophylaxis against Q fever under conditions of mandatory registration of this disease.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14453521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epidemiology and significance of Q fever in Czechoslovakia.","authors":"J Rehácek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Over the last 35 years most parts of Czechoslovakia have experienced Q fever. Of particular note were outbreaks in factories processing imported cotton, wool and hides that had been contaminated in their countries of origin. Some areas of the southern part of Central Slovakia became a natural focus of Q fever with the Dermacentor marginatus tick being the main vector of coxiellae. Currently, Coxiella (C.) burnetii infection appears to be dormant in the country since only sporadic cases are encountered. Cattle vaccination practiced since 1980 may have contributed to the decreasing incidence of human Q fever.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 1","pages":"16-9"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14451576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epidemiology and significance of Q fever in Hungary.","authors":"M Rády, R Glávits, G Nagy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In Hungary, Coxiella (C.) burnetii infections were diagnosed for the first time in 1956 in a few dairy and sheep farms. Q fever associated with abortion was first demonstrated in 1983 during laboratory diagnostic examination of fetuses and placentae. From case history data and on-the-spot investigations it is clear that in cattle stocks abortion caused by C. burnetii occurs sporadically, whereas in sheep flocks numerous abortions may take place within a short time. Losses caused by Q fever on the affected large-scale farms amount to 5-15%, which arise partly from reduced calf or lamb crop and partly from the death of offspring born with poor viability and being highly susceptible to neonatal disease. Altogether 87 cases of human disease caused by C. burnetii have been reported in the Hungarian literature. Instead of typical pneumonia, in recent years sporadic cases of granulomatous hepatitis were a constant finding accompanied by a characteristic blood picture and high titres of specific antibodies.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 1","pages":"10-5"},"PeriodicalIF":0.0,"publicationDate":"1987-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14559130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}