{"title":"[用薄层色谱法研究猪嗜血杆菌的氨基酸和碳水化合物代谢]。","authors":"E Balke, R Weiss, A Seipp","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of the present study was to investigate porcine reference and field strains of the species Haemophilus (H.) pleuropneumoniae and H. parasuis, as well as H. Taxon \"minor group\" and Taxon C on their amino acid and carbohydrate metabolism by thin layer chromatography. The 17 reference strains studied showed almost identical results within the different species and taxa in both, amino acid and carbohydrate metabolism patterns. Based on a few differing enzymatic reactions a reduced species- and taxon-specific reaction pattern could be established, which included L-alanine, L-citrulline and L-threonine of the amino acids as well as D-ribose, alpha-D-xylose, mannitol, trehalose, beta-melibiose and alpha-lactose of the carbohydrates. This differentiation system allowed a reliable identification of 7 field strains whereas 4 additional ones, hitherto pre-classified as H. parasuis, could not be associated with any of the above species and taxa.</p>","PeriodicalId":23821,"journal":{"name":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","volume":"267 4","pages":"495-505"},"PeriodicalIF":0.0000,"publicationDate":"1988-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Study of amino acid and carbohydrate metabolism of porcine strains of Haemophilus by thin layer chromatography].\",\"authors\":\"E Balke, R Weiss, A Seipp\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of the present study was to investigate porcine reference and field strains of the species Haemophilus (H.) pleuropneumoniae and H. parasuis, as well as H. Taxon \\\"minor group\\\" and Taxon C on their amino acid and carbohydrate metabolism by thin layer chromatography. The 17 reference strains studied showed almost identical results within the different species and taxa in both, amino acid and carbohydrate metabolism patterns. Based on a few differing enzymatic reactions a reduced species- and taxon-specific reaction pattern could be established, which included L-alanine, L-citrulline and L-threonine of the amino acids as well as D-ribose, alpha-D-xylose, mannitol, trehalose, beta-melibiose and alpha-lactose of the carbohydrates. This differentiation system allowed a reliable identification of 7 field strains whereas 4 additional ones, hitherto pre-classified as H. parasuis, could not be associated with any of the above species and taxa.</p>\",\"PeriodicalId\":23821,\"journal\":{\"name\":\"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology\",\"volume\":\"267 4\",\"pages\":\"495-505\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Mikrobiologie, und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用薄层色谱法对猪胸膜肺炎嗜血杆菌、副猪嗜血杆菌、猪“小群”嗜血杆菌分类群和C分类群的氨基酸和碳水化合物代谢进行了研究。所研究的17个参考菌株在不同种类和分类群中的氨基酸和碳水化合物代谢模式几乎相同。基于几种不同的酶促反应,可以建立一种还原的物种和分类特异性反应模式,包括氨基酸的l -丙氨酸、l -瓜氨酸和l -苏氨酸,以及碳水化合物的d -核糖、α - d -木糖、甘露醇、海藻糖、β -美利二糖和α -乳糖。该系统鉴定了7个田间菌株,而另外4个已被预分类为副猪血蜱的菌株与上述任何一个种和分类群都没有关联。
[Study of amino acid and carbohydrate metabolism of porcine strains of Haemophilus by thin layer chromatography].
The aim of the present study was to investigate porcine reference and field strains of the species Haemophilus (H.) pleuropneumoniae and H. parasuis, as well as H. Taxon "minor group" and Taxon C on their amino acid and carbohydrate metabolism by thin layer chromatography. The 17 reference strains studied showed almost identical results within the different species and taxa in both, amino acid and carbohydrate metabolism patterns. Based on a few differing enzymatic reactions a reduced species- and taxon-specific reaction pattern could be established, which included L-alanine, L-citrulline and L-threonine of the amino acids as well as D-ribose, alpha-D-xylose, mannitol, trehalose, beta-melibiose and alpha-lactose of the carbohydrates. This differentiation system allowed a reliable identification of 7 field strains whereas 4 additional ones, hitherto pre-classified as H. parasuis, could not be associated with any of the above species and taxa.