Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan最新文献_第5页

[Bio-nanoparticle: Making and Measurement]. [生物纳米颗粒：制造与测量]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00186-3

Eisuke Kanao

引用次数: 0

[Performance Evaluation of High-performance Liquid Chromatography Instrument in Determining the Serum Concentration of Linezolid]. 高效液相色谱法测定血清利奈唑胺浓度的性能评价

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00213

Naoaki Hashimoto, Shino Tsuda, Tomoka Kusama, Yuji Mukai, Ryuto Shioya, Mikiro Kato, Norihiko Terada, Miyuki Matsushita, Satoru Morikawa, Hideki Hashimoto, Masato Homma, Hiromichi Suzuki

{"title":"[Performance Evaluation of High-performance Liquid Chromatography Instrument in Determining the Serum Concentration of Linezolid].","authors":"Naoaki Hashimoto, Shino Tsuda, Tomoka Kusama, Yuji Mukai, Ryuto Shioya, Mikiro Kato, Norihiko Terada, Miyuki Matsushita, Satoru Morikawa, Hideki Hashimoto, Masato Homma, Hiromichi Suzuki","doi":"10.1248/yakushi.24-00213","DOIUrl":"https://doi.org/10.1248/yakushi.24-00213","url":null,"abstract":"LM1010, a high-performance liquid chromatography system for therapeutic drug monitoring (TDM) of various agents in clinical practice, is currently approved in Japan. Unlike conventional systems, LM1010 can rapidly measure clinical samples, and data analysis is based on the absolute calibration curve method and fully automated. However, the analytical performance of the LM1010 for the measurement of serum concentrations of linezolid (LZD) has not yet been evaluated. In this study, we aimed to evaluate the analytical performance of LM1010 and confirm the long-term stability of LZD at -30°C. One hundred forty-eight serum samples were collected from 25 patients (17 men and 8 women) who received LZD. Serum concentrations of LZD were measured using LM1010 and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Three LM1010 instruments were used to confirm the differences in the measured values. A Passing-Bablok regression analysis demonstrated a strong correlation between LM1010 and LC-MS/MS (r=0.991), and comparable results were observed among the three LM1010 instruments (r=0.982-0.998). Bland-Altman analyses revealed that the average difference between LM1010 and LC-MS/MS methods was 1.7%, and similar results were observed among the three LM1010 instruments. The long-term stability of serum LZD in samples stored at -30°C for 48 weeks was also confirmed. These results suggest that LM1010 can be used for TDM of LZD in clinical practice.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 6","pages":"553-560"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144200216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Elucidation of the Mechanisms of RNA Diseases and Manipulation of RNA]. [RNA疾病机制的阐明和RNA的操作]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00209-F

Miki Imanishi, Hitomi Tsuiji

引用次数: 0

[Identification of Food-derived Bioactive Components with Physiological Effects]. [具有生理作用的食源性生物活性成分的鉴定]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00173-2

Jun Inoue

{"title":"[Identification of Food-derived Bioactive Components with Physiological Effects].","authors":"Jun Inoue","doi":"10.1248/yakushi.24-00173-2","DOIUrl":"https://doi.org/10.1248/yakushi.24-00173-2","url":null,"abstract":"Food-derived components with physiological effects have been attracting attention in recent years, and studies have comprehensively analyzed these components. In this study, we sought to identify food components with functional properties for the prevention and improvement of metabolic syndrome. We performed a luciferase reporter assay using fatty acid synthase (FAS) and low-density lipoprotein receptor (LDL) receptor gene promoters. Naturally occurring isothiocyanate sulforaphane impaired FAS promoter activity and reduced sterol regulatory element-binding protein (SREBP) target gene expression in human hepatoma Huh-7 cells. Sulforaphane reduced SREBP proteins by promoting the degradation of the SREBP precursor. Furthermore, we screened LDL receptor promoter effectors and observed that extract from sweet cherry peduncles induces LDL receptor gene promoter activity. Several analytical and chemical methods revealed that chrysin 7O-β-D-glucopyranoside in cherry peduncle extract stimulated LDL receptor gene promoter activity. Thus, this comprehensive search for components that alter the expression of genes associated with lipid metabolism led to the discovery of new functions of food components.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 1","pages":"23-28"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Potential Analysis of Flavoring Agents as Novel Functional Foods]. 调味剂作为新型功能食品的潜力分析

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00173-1

Takuya Yamashita, Kazuya Nagano

引用次数: 0

[Disease Research and Drug Development Pioneered by Female Researchers with a Focus on the Liver]. [疾病研究和药物开发由女性研究人员开创，重点是肝脏]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00177-F

Yukiko Ueyama-Toba, Kahori Shimizu

引用次数: 0

[Current Situation and Challenges of Regulation and Analytical Methods for Residual Pesticides, Veterinary Drugs and Mycotoxins in Foods]. [食品中残留农药、兽药和真菌毒素监管与分析方法的现状与挑战]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00164-F

Takaaki Taguchi, Tomoaki Tsutsumi

引用次数: 0

[Usefulness of SPLINE-Deconvolution Method to Analyze Absorption Kinetics of Fentanyl Released from Systemically Acting Fentanyl Patch]. [样条-反褶积法分析全身芬太尼贴剂释放的芬太尼吸收动力学的有效性]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00193

Noriko Hirota, Hiroki Hirayama

{"title":"[Usefulness of SPLINE-Deconvolution Method to Analyze Absorption Kinetics of Fentanyl Released from Systemically Acting Fentanyl Patch].","authors":"Noriko Hirota, Hiroki Hirayama","doi":"10.1248/yakushi.24-00193","DOIUrl":"https://doi.org/10.1248/yakushi.24-00193","url":null,"abstract":"Durotep®MT Patch (DMTP) (Janssen Pharmaceutical K.K., Tokyo) is a systemically acting narcotic patch containing fentanyl. It is applied transdermally and removed 72 h after applying. The range of effective serum fentanyl concentrations is very narrow (0.3-3 ng/mL). Therefore, analysis of the absorption kinetics (ABK) is especially important. We studied ABK for fentanyl from DMTP 16.8 mg. There are 5 analytical methods to obtain ABK: The estimated mean absorbed rate at steady state (EMARSS) obtained using the residual method (standard value), absolute bioavailability method, Wagner-Nelson method, 3-compartment model Loo-Riegelman method (3LR), and SPLINE-Deconvolution method (SDecon), yielding results of 0.104, 0.119, 0.114, 0.109, and 0.103 mg/h respectively. EMARSS for 3LR and SDecon were nearly equal to the standard value. The absorbed rate for fentanyl with elapsed time by SDecon indicated an amplitude of 3 times with about a 20 h period at 10.7-72 h and the increase in the absorption rate began before the increase in the serum concentration when DMTP was applied transdermally. This phenomenon indicates that the mean absorption rate was constant; thus, an absorption steady state was reached. The actual mean absorption rate during this period was 0.0924 mg/h (range: 0.0594-0.131). The cumulative absorption ratio increased gradually with only a small amplitude, and bioavailability was 44.2%. From our results, SPLINE-Deconvolution is the most suitable method to assess fentanyl absorption kinetics of DMTP.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 5","pages":"461-468"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Elucidating the Mechanisms of Lipid Accumulation in the Liver and Evaluating Potential Drug Targets]. [阐明肝脏脂质积聚机制及评估潜在药物靶点]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00206

Takayuki Koga

引用次数: 0

[Application of Human Liver Organoids for Pharmaceutical Research]. [人肝类器官在药物研究中的应用]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00177-4

Yukiko Ueyama-Toba, Yanran Tong, Hiroyuki Mizuguchi

{"title":"[Application of Human Liver Organoids for Pharmaceutical Research].","authors":"Yukiko Ueyama-Toba, Yanran Tong, Hiroyuki Mizuguchi","doi":"10.1248/yakushi.24-00177-4","DOIUrl":"10.1248/yakushi.24-00177-4","url":null,"abstract":"Human liver organoids are expected to be a hepatocyte source for preclinical in vitro studies of drug metabolism and disposition. Although these organoids show long-term proliferation, their hepatic functions remain low. Therefore, it is necessary to enhance the hepatic functions of primary human hepatocyte (PHH)-derived organoids. Here, we propose a novel method for two dimensional (2D)-cultured hepatic differentiation from PHH-derived organoids. PHH-derived organoids were established from cryopreserved PHHs. When cultured under a 2D condition, the single cells from PHH-derived organoids were seeded on collagen type I-coated plates. Then, optimal conditions for hepatic differentiation were screened using several compounds, cytokines and growth factors. Based on the results of the screening, we determined the 2D-cultured hepatic differentiation method from PHH-derived organoids. Hepatic gene expressions in PHH-derived organoids-derived hepatocytes (Org-HEPs) were greatly increased, compared to those in PHH-derived organoids. An RNA-seq analysis showed that gene expressions related to pharmacokinetics were upregulated in Org-HEPs compared to PHH-derived organoids. The metabolic activities of CYP1A2, CYP2C8, CYP2E1 and CYP3A4 were at levels comparable to those in PHHs. We also treated Org-HEPs and PHHs with hepatotoxic drugs, such as acetaminophen, troglitazone, amiodarone and clozapine. The cell viability of Org-HEPs was almost the same as that of PHHs. These results suggested that PHH-derived organoids could be differentiated into highly functional hepatocytes in 2D culture, and Org-HEPs could be used for hepatotoxicity tests. Thus, Org-HEPs will be useful for pharmaceutical research.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 3","pages":"189-194"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0