Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan最新文献_第4页

[Molecular Mechanisms by Which Polyunsaturated Fatty Acids Suppress the Pathogenesis and Progression of NAFLD]. [多不饱和脂肪酸抑制非酒精性脂肪肝发病和恶化的分子机制]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00177-2

Misa Ando, Hyeon-Cheol Lee-Okada, Takehiko Yokomizo

{"title":"[Molecular Mechanisms by Which Polyunsaturated Fatty Acids Suppress the Pathogenesis and Progression of NAFLD].","authors":"Misa Ando, Hyeon-Cheol Lee-Okada, Takehiko Yokomizo","doi":"10.1248/yakushi.24-00177-2","DOIUrl":"https://doi.org/10.1248/yakushi.24-00177-2","url":null,"abstract":"In recent years, the number of people suffering from lifestyle diseases such as hyperlipidemia and fatty liver disease has increased rapidly due to westernization of dietary patterns. Among fatty liver diseases, those that are not caused by alcohol are referred to as nonalcoholic fatty liver disease (NAFLD). Some NAFLD can progress to nonalcoholic steatohepatitis (NASH), and further progression of NAFLD can lead to cirrhosis and liver cancer. Although numerous studies have demonstrated the efficacy of dietary polyunsaturated fatty acids (PUFAs), particularly omega-3 PUFAs eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), against NAFLD, the detailed mechanisms by which these PUFAs exert their protective effects on the pathogenesis and progression of NAFLD are not well understood. Recent studies using knockout mouse models and genome-wide association studies have suggested a potential role for the enzymes responsible for the biosynthesis of PUFAs (FADS1, FADS2, ELOVL2, and ELOVL5) and their incorporation into phospholipids (LPCAT3/MBOAT5/LPLAT12 and LPIAT1/MBOAT7/LPLAT11) in the development of NAFLD. In this review, we summarize recent findings on the association of NAFLD and PUFAs with a focus on PUFA biosynthetic and metabolic enzymes to discuss the potential role of PUFAs in the prevention of NAFLD.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 3","pages":"177-182"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143538016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The Roles and Operations of Registered Conformity Assessment Body].

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00164-3

Takayuki Asakura

{"title":"[The Roles and Operations of Registered Conformity Assessment Body].","authors":"Takayuki Asakura","doi":"10.1248/yakushi.24-00164-3","DOIUrl":"https://doi.org/10.1248/yakushi.24-00164-3","url":null,"abstract":"As of August 2023, 99 organizations were registered as conformity assessment bodies by the Ministry of Health, Labour, and Welfare in accordance with Article 33 of the Food Sanitation Act. These registered organizations are authorized to conduct inspections of imported food as well as domestic expropriation inspections. In recent years, we have actively participated in a project to develop new test methods, aiming to enhance our contribution as a registered testing organization and to maintain food hygiene by widely applying these developed methods. There is a need to develop new test methods for pesticides and veterinary medicines used in Japan, as well as for several drugs used internationally. Previously, gas chromatography and high-performance liquid chromatography were the standard methods, and only parent compounds were analyzed. Recently, the demand for test methods applicable not only to agricultural products but also to livestock and marine foods has increased, necessitating the analysis of metabolites in addition to parent compounds. Although developing analytical methods to detect certain metabolites has been challenging, advancements in the variety of separation and pretreatment columns, along with the widespread use of LC-MS/MS and GC-MS/MS techniques, have enabled the development of test methods for many compounds. In this study, we introduce a method to solve the challenges and problems associated with test method development.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 2","pages":"105-108"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Anti-skin Aging Effects of Kale-derived Exosome-like Nanoparticles]. [甘蓝衍生的外泌体样纳米颗粒的抗皮肤衰老作用]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00173-3

Shigeru Katayama

{"title":"[Anti-skin Aging Effects of Kale-derived Exosome-like Nanoparticles].","authors":"Shigeru Katayama","doi":"10.1248/yakushi.24-00173-3","DOIUrl":"https://doi.org/10.1248/yakushi.24-00173-3","url":null,"abstract":"In an aging society, there is a growing interest in functional foods that offer anti-aging benefits. Food-derived bioactive compounds such as carotenoids and polyphenols can enhance skin elasticity and delay aging. However, the mechanisms by which these orally ingested compounds directly impact the skin are not fully understood. Recent studies on exosomes have suggested significant physiological functions, including their potential for intercellular communication. Similar to mammalian exosomes, plant-derived exosomes are known for their functional roles, including cross-kingdom communication, and their ability to target specific organs in animal models as delivery vehicles. The authors have been investigating the anti-aging effects of kale and have previously reported its benefits on cognitive function and skin aging in mouse models. Long-term oral administration of glucoraphanin-enriched kale suppresses the senescence symptoms in skin and hair and increases type I collagen and antioxidant enzyme expression in skin tissues, indicating its role in promoting skin health. Exosome-like nanoparticles (ELNs) from glucoraphanin-enriched kale appear to modulate the expression of extracellular matrix-related genes. Kale-derived ELNs exhibit great potential for ameliorating skin aging, suggesting their ability to promote skin health through targeted cellular mechanisms and supporting their use as an active natural compound in nutraceuticals and functional beverages.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 1","pages":"29-33"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Formulation Design that Contributes to the Development of Pediatric-friendly Drugs]. [促进儿科友好型药物开发的配方设计]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00171-3

Tsutomu Harada

{"title":"[Formulation Design that Contributes to the Development of Pediatric-friendly Drugs].","authors":"Tsutomu Harada","doi":"10.1248/yakushi.24-00171-3","DOIUrl":"10.1248/yakushi.24-00171-3","url":null,"abstract":"Age-appropriate pediatric formulations are needed to achieve optimal medication adherence in children. In Europe and the United States, mini-tablets (MTs) have garnered interest as a new pediatric formulation that is easier to swallow than liquid formulations (LFs). In Japan, fine granules (FGs) are also common for children. Acceptability of a medicine is likely to have significant impact on pediatric adherence. We examined the acceptability of drug-free MTs and compared it with that of FGs and LFs in children aged 6 months to 8 years who were visiting a pediatric department in Showa University Hospital. The primary endpoint was the measure of acceptability by observation of healthcare professionals according to pre-defined evaluation criteria. We compared the percentage of children who could swallow MTs without chewing with that of children who could swallow FGs or LFs without leftover. MTs showed lower swallowability compared to FGs and LFs in children aged 2-6 years, likely because children tend to chew on MTs. In contrast, significantly more children aged 6-11 months could swallow the MTs than those who could swallow all the FGs and LFs. No significant differences were observed in children aged 12-23 months. Palatability and swallowability are important for acceptability. Parents of children aged 6-23 months rated palatability after taking the three formulations. We combined the swallowability and palatability results into a new acceptability analysis. MTs did not differ significantly from FGs and LFs in acceptability for infants, but this new endpoint is useful for evaluating oral pediatric formulations.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 4","pages":"289-297"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chemistry and Biological Activity of Diterpenoid Alkaloids]. [二萜类生物碱的化学和生物活性]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00211

Koji Wada

{"title":"[Chemistry and Biological Activity of Diterpenoid Alkaloids].","authors":"Koji Wada","doi":"10.1248/yakushi.24-00211","DOIUrl":"10.1248/yakushi.24-00211","url":null,"abstract":"Diterpenoid alkaloids are major pharmaceutically active constituents of Aconitum plants. In phytochemical investigations on Aconitum japonicum subsp. subcuneatum, Aconitum yesoense var. macroyesoens, and Delphinium elatum cv. Pacific Giant (Ranunclaceae), the structures of isolated C19- and C20-diterpenoid alkaloids were elucidated. Three aconitine-type C19-diterpenoid alkaloids, jesaconitine, aconitine, and mesaconitine, which are main components of A. japonicum subsp. subcuneatum, are significantly toxic to the central nervous system. However, lycoctonine-type C19-diterpenoid alkaloids and C20-diterpenoid alkaloids are less toxic. Several diterpenoid alkaloids from the genera Aconitum and Delphinium and their derivatives exhibited slight cytotoxic activity against several human tumor cell lines [A549 (lung carcinoma), DU145 (prostate carcinoma), MDA-MB-231 (triple-negative breast cancer), MCF-7 (estrogen receptor-positive, HER2-negative breast cancer), KB (identical to cervical carcinoma HeLa derived AV-3 cell line), and multidrug-resistant (MDR) subline KB-VIN]. In our course of studies on synthetic derivatives of the C19-diterpenoid alkaloids delcosine and delpheline and the C20-diterpenoid alkaloids lucidusculine, pseudokobusine, and kobusine, we found several derivatives showing significant cytotoxic activity and, thus, providing promising new leads for further development as antitumor agents. Notably, several diterpenoid alkaloids were more potent against MDR subline KB-VIN cells than the parental drug-sensitive KB cells. Among non-cytotoxic synthetic analogues, several lycoctonine-type C19-diterpenoid alkaloid derivatives effectively and significantly sensitized MDR cells to three anticancer drugs, paclitaxel, vincristine, and doxorubicin.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 4","pages":"313-349"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Inspection of Residual Veterinary Drugs at Tokyo Metropolitan Institute of Public Health: Bioassay and LC-MS/MS Analysis].

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00164-4

Maki Kanda

{"title":"[Inspection of Residual Veterinary Drugs at Tokyo Metropolitan Institute of Public Health: Bioassay and LC-MS/MS Analysis].","authors":"Maki Kanda","doi":"10.1248/yakushi.24-00164-4","DOIUrl":"https://doi.org/10.1248/yakushi.24-00164-4","url":null,"abstract":"Veterinary drugs are used worldwide to prevent and treat diseases and promote growth in animals, fisheries, and beekeeping. Despite their effectiveness, the illegal and improper use of these drugs can result in livestock and fishery products, potentially impacting human health by causing allergic reactions, cytotoxicity, and antimicrobial resistance. To mitigate these adverse effects, the Japanese government established a positive list system in 2006. Maximum residue levels (MRLs) have been defined for approximately 300 veterinary drugs. The regulation of certain antimicrobial drugs without defined MRLs uses in zero tolerance. Residual veterinary drugs in meat, fish, eggs, milk, and honey are inspected to ensure compliance with legal limits. Initially, samples are screened using bioassay and LC-MS/MS. If residues are detected, they are confirmed and quantified using more precise methods. We have detailed the detection of residual norfloxacin in honey. Additionally, we are currently developing new analytical methods. This study introduces an analytical method for the residue screening of sulfonamides and quinolones. After residue detection by bioassay, the same test solutions were analyzed by LC-MS/MS for accurate identification and quantification. This method has also proven to be more environmentally friendly compared to other quantitative methods. We present the ingenuity used to realize the combined method, performance evaluation results, examples of applications to actual samples, and future prospects.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 2","pages":"109-115"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Role of Median Raphe Serotonergic Neurons in Positive and Negative Information Processing].

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00150

Hiroyuki Kawai

{"title":"[Role of Median Raphe Serotonergic Neurons in Positive and Negative Information Processing].","authors":"Hiroyuki Kawai","doi":"10.1248/yakushi.24-00150","DOIUrl":"https://doi.org/10.1248/yakushi.24-00150","url":null,"abstract":"Serotonergic neurons play a critical role in processing reward and aversive information. Rewarding stimuli activate serotonergic neurons in the dorsal raphe nucleus (DRN), whereas optogenetic activation of DRN serotonergic neurons induces reward-like effects. However, the pharmacological enhancement of serotonin neurotransmission does not induce rewarding or aversive effects. These findings suggest the presence of another serotonergic neuron that plays a role opposite to that of the DRN in processing reward and aversion information. Previous reports suggested that the median raphe nucleus (MRN) processes negative emotional stimuli. To elucidate the function of MRN serotonergic neurons in these processes, we recorded the changes in serotonergic activity in mice in response to rewarding and aversive stimuli. We also used optogenetic manipulation to determine whether these changes could induce rewarding and aversive behaviors. The activity of MRN serotonergic neurons decreased in response to rewarding stimuli and increased after aversive stimuli. Optogenetic inhibition of MRN serotonergic neurons induced reward-related behavior, while optogenetic stimulation induced aversion-related behavior. Furthermore, we found that the projection pathway from MRN serotonergic neurons to the interpeduncular nucleus is crucial for these processes. These results indicate that MRN serotonergic neurons play a pivotal role in processing reward and aversive information, functioning oppositely to DRN neurons.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 2","pages":"79-84"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Challenges in Development of Appropriate Drugs for Children]. [开发适合儿童的药物面临的挑战]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00171-F

Hiroyuki Kawagishi, Yasunari Kanda

引用次数: 0

[Development of High-Performance Countercurrent Chromatography and Its Application in the Separation of Bioactive Compounds]. [高效逆流色谱法的发展及其在生物活性化合物分离中的应用]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00187

Kazufusa Shinomiya

{"title":"[Development of High-Performance Countercurrent Chromatography and Its Application in the Separation of Bioactive Compounds].","authors":"Kazufusa Shinomiya","doi":"10.1248/yakushi.24-00187","DOIUrl":"10.1248/yakushi.24-00187","url":null,"abstract":"Countercurrent chromatography (CCC) is a form of liquid-liquid partition chromatography that eliminates the solid support used in column chromatography. This allows the recovery of all samples subjected to CCC separation of bioactive components without denaturation and adsorption caused by interacting with the column matrix. The CCC apparatus requires numerous and continuous partitioning processes composed of the sufficient mixing of the two-phase solvent system, separating into two liquid phases, and moving the mobile phase. The present paper introduces my studies over 35 years on the development and improvement of CCC including the rotation behavior of the coiled column, column configuration, tube design, and two-phase solvent system followed by the application to the separation of bioactive compounds. Among the CCC instruments developed or improved in those studies, the floor-standing type of cross-axis CCC was first domestically produced and prompted the fabrication of benchtop type small-scale cross-axis CCC to achieve satisfactory separation of proteins and enzymes without loss of their bioactivity using aqueous two-phase solvent systems. The coil satellite centrifuge designed and fabricated in our laboratory enabled sufficient separation using a two-phase solvent system after adjusting the suitable rotation speed combination of the sun axis, planet axis, and satellite axis. Better partition efficiency was achieved using an eccentric coil for the analytical-scale and multilayer coil for the preparative-scale. Notably, the multilayer coil wound with long-pressed locular tubing increased the peak resolution within shortened separation times. The high-performance of the CCC apparatus developed will expand the ability to reveal the mechanisms of cell particles.","PeriodicalId":23810,"journal":{"name":"Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan","volume":"145 4","pages":"299-312"},"PeriodicalIF":0.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Basic and Clinical Research on Placental Function for Drug Development Research]. [药物开发研究中胎盘功能的基础与临床研究]。

IF 0.3 4区医学

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan Pub Date : 2025-01-01 DOI: 10.1248/yakushi.24-00174-F

Kazuma Higashisaka, Mikihiro Yoshie

引用次数: 0