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World Journal of Microbiology and Biotechnology最新文献

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Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system 在 Pichia pastoris 表达系统中进行蛋白质工程的当前成就、策略、障碍和克服的挑战
World Journal of Microbiology and Biotechnology Pub Date : 2023-12-08 DOI: 10.1007/s11274-023-03851-6
Azadeh Eskandari, Nima Ghahremani Nezhad, Thean Chor Leow, Mohd Basyaruddin Abdul Rahman, Siti Nurbaya Oslan
{"title":"Current achievements, strategies, obstacles, and overcoming the challenges of the protein engineering in Pichia pastoris expression system","authors":"Azadeh Eskandari, Nima Ghahremani Nezhad, Thean Chor Leow, Mohd Basyaruddin Abdul Rahman, Siti Nurbaya Oslan","doi":"10.1007/s11274-023-03851-6","DOIUrl":"https://doi.org/10.1007/s11274-023-03851-6","url":null,"abstract":"<p>Yeasts serve as exceptional hosts in the manufacturing of functional protein engineering and possess industrial or medical utilities. Considerable focus has been directed towards yeast owing to its inherent benefits and recent advancements in this particular cellular host. The <i>Pichia pastoris</i> expression system is widely recognized as a prominent and widely accepted instrument in molecular biology for the purpose of generating recombinant proteins. The advantages of utilizing the <i>P. pastoris</i> system for protein production encompass the proper folding process occurring within the endoplasmic reticulum (ER), as well as the subsequent secretion mediated by Kex2 as a signal peptidase, ultimately leading to the release of recombinant proteins into the extracellular environment of the cell. In addition, within the <i>P. pastoris</i> expression system, the ease of purifying recombinant protein arises from its restricted synthesis of endogenous secretory proteins. Despite its achievements, scientists often encounter persistent challenges when attempting to utilize yeast for the production of recombinant proteins. This review is dedicated to discussing the current achievements in the usage of <i>P. pastoris</i> as an expression host. Furthermore, it sheds light on the strategies employed in the expression system and the optimization and development of the fermentative process of this yeast. Finally, the impediments (such as identifying high expression strains, improving secretion efficiency, and decreasing hyperglycosylation) and successful resolution of certain difficulties are put forth and deliberated upon in order to assist and promote the expression of complex proteins in this prevalent recombinant host.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and distribution pattern of AmpC β-lactamases in ESBL producing clinical isolates of Klebsiella spp. in parts of Assam, India 印度阿萨姆邦部分地区产生 ESBL 的克雷伯氏菌临床分离株中 AmpC β-内酰胺酶的流行和分布模式
World Journal of Microbiology and Biotechnology Pub Date : 2023-12-08 DOI: 10.1007/s11274-023-03846-3
Indrani Gogoi, Shyamalima Saikia, Mohan Sharma, Amos Oloo Onyango, Minakshi Puzari, Pankaj Chetia
{"title":"Prevalence and distribution pattern of AmpC β-lactamases in ESBL producing clinical isolates of Klebsiella spp. in parts of Assam, India","authors":"Indrani Gogoi, Shyamalima Saikia, Mohan Sharma, Amos Oloo Onyango, Minakshi Puzari, Pankaj Chetia","doi":"10.1007/s11274-023-03846-3","DOIUrl":"https://doi.org/10.1007/s11274-023-03846-3","url":null,"abstract":"<p>The production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases is the most common explanation of multidrug resistance in clinical isolates of <i>Klebsiella</i> spp. In the present study, a total of 160 isolates of <i>Klebsiella</i> spp. were procured from the DBT-NER project with ethical clearance no. DU/Dib/ECBHR(Human)/2021-22/02). These were collected from various health settings of Assam and identified as drug-resistant. The isolates were screened for antibiotic susceptibility and phenotypic tests were performed on multidrug resistant isolates to confirm ESBL and AmpC β-lactamases production. The distribution pattern of ESBL and AmpC β-lactamase genotype was investigated by polymerase chain reaction (PCR). The results showed that among 107 multidrug-resistant (MDR) isolates of <i>Klebsiella</i> spp., 67.28% of isolates were ESBL producers and 56.07% were potential AmpC producers. The PCR results revealed that <i>bla</i><sub>CTX−M</sub> was the most prevalent ESBL genotype. Among the ESBL producers, 11.11% of isolates showed co-occurrence with plasmid-mediated AmpC β lactamases genotype which indicated the high prevalence of ESBL and AmpC co-producers in <i>K. pneumoniae</i> and <i>K. oxytoca</i>, suggesting the possibility of serious public health concerns. Therefore, it is crucial to regularly monitor the spread of multidrug resistance among clinical isolates.</p>","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138556790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cytotoxicity and anti-biofilm activities of biogenic cadmium nanoparticles and cadmium nitrate: a preliminary study 生物源性纳米镉和硝酸镉的细胞毒性及抗生物膜活性的初步研究
World Journal of Microbiology and Biotechnology Pub Date : 2022-10-27 DOI: 10.1007/s11274-022-03418-x
Mahboubeh Adeli-sardou, Mojtaba Shakibaie, H. Forootanfar, Fereshteh Jabari-Morouei, Soudabe Riahi-Madvar, Sima-Sadat Ghafari-Shahrbabaki, M. Mehrabani
{"title":"Cytotoxicity and anti-biofilm activities of biogenic cadmium nanoparticles and cadmium nitrate: a preliminary study","authors":"Mahboubeh Adeli-sardou, Mojtaba Shakibaie, H. Forootanfar, Fereshteh Jabari-Morouei, Soudabe Riahi-Madvar, Sima-Sadat Ghafari-Shahrbabaki, M. Mehrabani","doi":"10.1007/s11274-022-03418-x","DOIUrl":"https://doi.org/10.1007/s11274-022-03418-x","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74982035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cacti as low-cost substrates to produce L-asparaginase by endophytic fungi 仙人掌作为内生真菌生产l -天冬酰胺酶的低成本底物
World Journal of Microbiology and Biotechnology Pub Date : 2022-10-27 DOI: 10.1007/s11274-022-03420-3
Leticia Francisca da Silva, Ana Patrícia Sousa Lopes de Pádua, Layanne de Oliveira Ferro, G. S. Agamez-Montalvo, J. Bezerra, K. Moreira, C. M. de Souza-Motta
{"title":"Cacti as low-cost substrates to produce L-asparaginase by endophytic fungi","authors":"Leticia Francisca da Silva, Ana Patrícia Sousa Lopes de Pádua, Layanne de Oliveira Ferro, G. S. Agamez-Montalvo, J. Bezerra, K. Moreira, C. M. de Souza-Motta","doi":"10.1007/s11274-022-03420-3","DOIUrl":"https://doi.org/10.1007/s11274-022-03420-3","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"39 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80954608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Interference of bio-control Trichoderma to enhance physical and physiological strength of sugarcane during Pokkah boeng infection 生物防治木霉的干扰提高甘蔗在白桦侵染期间的生理生理强度
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-16 DOI: 10.1007/s11274-022-03319-z
R. Tiwari, K. Chandra, S. Shukla, V. P. Jaiswal, N. Amaresan, A. Srivastava, A. Gaur, D. Sahni, R. Tiwari
{"title":"Interference of bio-control Trichoderma to enhance physical and physiological strength of sugarcane during Pokkah boeng infection","authors":"R. Tiwari, K. Chandra, S. Shukla, V. P. Jaiswal, N. Amaresan, A. Srivastava, A. Gaur, D. Sahni, R. Tiwari","doi":"10.1007/s11274-022-03319-z","DOIUrl":"https://doi.org/10.1007/s11274-022-03319-z","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84722323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Genomic characterization of denitrifying methylotrophic Pseudomonas aeruginosa strain AAK/M5 isolated from municipal solid waste landfill soil 城市垃圾填埋场土壤中反硝化甲基营养化铜绿假单胞菌AAK/M5的基因组特征
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-16 DOI: 10.1007/s11274-022-03311-7
A. K. Singh, R. Gupta, H. Purohit, A. Khardenavis
{"title":"Genomic characterization of denitrifying methylotrophic Pseudomonas aeruginosa strain AAK/M5 isolated from municipal solid waste landfill soil","authors":"A. K. Singh, R. Gupta, H. Purohit, A. Khardenavis","doi":"10.1007/s11274-022-03311-7","DOIUrl":"https://doi.org/10.1007/s11274-022-03311-7","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84355927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
In vitro compatibility of Brassicaceae extracts with nematophagous fungi and their effects against Nacobbus celatus 芸苔科提取物与噬线虫真菌的体外配伍性及其对青花nacobus celatus的防治作用
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-15 DOI: 10.1007/s11274-022-03318-0
Anacleto Sosa, N. Girardi, L. Rosso, M. Etcheverry, M. A. Passone
{"title":"In vitro compatibility of Brassicaceae extracts with nematophagous fungi and their effects against Nacobbus celatus","authors":"Anacleto Sosa, N. Girardi, L. Rosso, M. Etcheverry, M. A. Passone","doi":"10.1007/s11274-022-03318-0","DOIUrl":"https://doi.org/10.1007/s11274-022-03318-0","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"26 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82835136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Comparative study on inhibitory effects of ferulic acid and p-coumaric acid on Salmonella Enteritidis biofilm formation 阿魏酸和对香豆酸对肠炎沙门氏菌生物膜形成抑制作用的比较研究
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-14 DOI: 10.1007/s11274-022-03317-1
Jingguo Xu, Hui Hu, Jingyu Chen, Yansong Xue, Bekhzod Kodirkhonov, B. Han
{"title":"Comparative study on inhibitory effects of ferulic acid and p-coumaric acid on Salmonella Enteritidis biofilm formation","authors":"Jingguo Xu, Hui Hu, Jingyu Chen, Yansong Xue, Bekhzod Kodirkhonov, B. Han","doi":"10.1007/s11274-022-03317-1","DOIUrl":"https://doi.org/10.1007/s11274-022-03317-1","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86884351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Characteristics of the microbiota and metabolic profile of high-temperature Daqu with different grades 不同品级高温大曲菌群特征及代谢特征
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-14 DOI: 10.1007/s11274-022-03303-7
Yuandi Zhang, Fang Ding, Yi Shen, Wei Cheng, Yansong Xue, B. Han, Xiaoxue Chen
{"title":"Characteristics of the microbiota and metabolic profile of high-temperature Daqu with different grades","authors":"Yuandi Zhang, Fang Ding, Yi Shen, Wei Cheng, Yansong Xue, B. Han, Xiaoxue Chen","doi":"10.1007/s11274-022-03303-7","DOIUrl":"https://doi.org/10.1007/s11274-022-03303-7","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77283214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Genomic attributes of thermophilic and hyperthermophilic bacteria and archaea 嗜热性和超嗜热性细菌和古细菌的基因组属性
World Journal of Microbiology and Biotechnology Pub Date : 2022-06-13 DOI: 10.1007/s11274-022-03327-z
D. Verma, Vinod Kumar, T. Satyanarayana
{"title":"Genomic attributes of thermophilic and hyperthermophilic bacteria and archaea","authors":"D. Verma, Vinod Kumar, T. Satyanarayana","doi":"10.1007/s11274-022-03327-z","DOIUrl":"https://doi.org/10.1007/s11274-022-03327-z","url":null,"abstract":"","PeriodicalId":23744,"journal":{"name":"World Journal of Microbiology and Biotechnology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87191952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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