World journal of methodology最新文献

{"title":"COVID-19 disease and autoimmune disorders: A mutual pathway.","authors":"Mohammed Al-Beltagi, Nermin Kamal Saeed, Adel Salah Bediwy","doi":"10.5662/wjm.v12.i4.200","DOIUrl":"10.5662/wjm.v12.i4.200","url":null,"abstract":"<p><p>Coronavirus disease 2019 (COVID-19) is a real challenge for humanity with high morbidity and mortality. Despite being primarily a respiratory illness, COVID-19 can affect nearly every human body tissue, causing many diseases. After viral infection, the immune system can recognize the viral antigens presented by the immune cells. This immune response is usually controlled and terminated once the infection is aborted. Nevertheless, in some patients, the immune reaction becomes out of control with the development of autoimmune diseases. Several human tissue antigens showed a strong response with antibodies directed against many severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins, such as SARS-CoV-2 S, N, and autoimmune target proteins. The immunogenic effects of SARS-CoV-2 are due to the sizeable viral RNA molecules with interrupted transcription increasing the pool of epitopes with increased chances of molecular mimicry and interaction with the host immune system, the overlap between some viral and human peptides, the viral induced-tissue damage, and the robust and complex binding between sACE-2 and SARS-CoV-2 S protein. Consequently, COVID-19 and its vaccine may trigger the development of many autoimmune diseases in a predisposed patient. This review discusses the mutual relation between COVID-19 and autoimmune diseases, their interactive effects on each other, the role of the COVID-19 vaccine in triggering autoimmune diseases, the factors affecting the severity of COVID-19 in patients suffering from autoimmune diseases, and the different ways to minimize the risk of COVID-19 in patients with autoimmune diseases.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 4","pages":"200-223"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7d/b4/WJM-12-200.PMC9350728.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33483471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of human leishmaniasis in Sudan: A systematic review and meta-analysis.","authors":"Musa Ahmed,&nbsp;Abdullah Abdulslam Abdullah,&nbsp;Idris Bello,&nbsp;Suad Hamad,&nbsp;Aboelgassim Bashir","doi":"10.5662/wjm.v12.i4.305","DOIUrl":"https://doi.org/10.5662/wjm.v12.i4.305","url":null,"abstract":"<p><strong>Background: </strong>There are three main forms of leishmaniasis in humans: cutaneous leishmaniasis (CL), visceral leishmaniasis (VL), and mucocutaneous leishmaniasis. The prevalence of human leishmaniasis varies widely in different countries and different regions of the same country. To date, there is no overall estimation of the prevalence of human leishmaniasis in Sudan.</p><p><strong>Aim: </strong>To determine the pooled prevalence of human leishmaniasis and the disease risk factors among Sudanese citizens.</p><p><strong>Methods: </strong>From all articles written in English or Arabic languages conducted before the 4th of August 2021 from [Scopus, Web of Science, PubMed, and MEDLINE, African Journals Online (AJOL), ResearchGate, direct Google search, Google Scholar, and universities websites], just 20 articles with a total of 230960 participants were eligible for this study. Data synthesis and analysis were done using STATA software, version 16. EndNote citation manager version X9.3.3 and <i>Reference Citation Analysis</i> (<i>RCA</i>) were used to remove the duplicated studies and manage the citation respectively.</p><p><strong>Results: </strong>The overall pooled prevalence of human leishmaniasis in Sudan was 21% (with confidence interval 12%-30%). CL was the most common type of leishmaniasis in Sudan, with a pooled prevalence of 26% followed by VL (18%). Nevertheless, the pooled prevalence of human leishmaniasis in Sudan was higher in males compared with females (60% <i>vs</i> 40%). The current results revealed that the people in the age group between 15 and 44 were the most affected group (60%), and central Sudan has the highest pooled prevalence of human leishmaniasis (27%) compared with other regions of Sudan. Finally, the prevalence of human leishmaniasis seems to decrease with time.</p><p><strong>Conclusion: </strong>This study showed that human leishmaniasis infection is still endemic in many regions in Sudan and highly prevalent in central and eastern Sudan, and CL is the most prevalent in the country. Males and adults were more susceptible to infection compared with females and children. However, the human leishmaniasis prevalence decreased relatively over time.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 4","pages":"305-318"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e7/57/WJM-12-305.PMC9350725.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33483469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rehabilitation in long COVID-19: A mini-review.","authors":"Raktim Swarnakar,&nbsp;Shiv Lal Yadav","doi":"10.5662/wjm.v12.i4.235","DOIUrl":"https://doi.org/10.5662/wjm.v12.i4.235","url":null,"abstract":"<p><p>We have been experiencing multiple waves of the coronavirus disease 2019 (COVID-19) pandemic. With these unprecedented waves, we have entered into an era of 'new normal'. This pandemic has enforced us to rethink the very basics of childhood learning: Habits, health etiquette, and hygiene. Rehabilitation has immense importance during this pandemic considering a few aspects. Multidisciplinary COVID-19 rehabilitation clinics are essential to address the demand. The equitable distribution of COVID-19 rehabilitation services for differently-abled individuals during the pandemic is an important aspect. Rehabilitation needs identification and further studies on various rehabilitation interventions are among the key unmet future research needs.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 4","pages":"235-245"},"PeriodicalIF":0.0,"publicationDate":"2022-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/12/b3/WJM-12-235.PMC9350732.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33484544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of diagnostic capacity and decision-making based on the 2015 American Thyroid Association ultrasound classification system.","authors":"Luis-Mauricio Hurtado-Lopez,&nbsp;Alfredo Carrillo-Muñoz,&nbsp;Felipe-Rafael Zaldivar-Ramirez,&nbsp;Erich Otto Paul Basurto-Kuba,&nbsp;Blanca-Estela Monroy-Lozano","doi":"10.5662/wjm.v12.i3.148","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.148","url":null,"abstract":"<p><strong>Background: </strong>This study evaluates the American Thyroid Association (ATA) ultrasound (US) classification system for the initial assessment of thyroid nodules to determine if it indeed facilitates clinical decision-making.</p><p><strong>Aim: </strong>To perform a systematic review and meta-analysis of the diagnostic value of the ATA US classification system for the initial assessment of thyroid nodules.</p><p><strong>Methods: </strong>In accordance with the PRISMA statement for diagnostic test accuracy, we selected articles that evaluated the 2015 ATA US pattern guidelines using a diagnostic gold standard. We analyzed these cases using traditional diagnostic parameters, as well as the threshold approach to clinical decision-making and decision curve analysis.</p><p><strong>Results: </strong>We reviewed 13 articles with 8445 thyroid nodules, which were classified according to 2015 ATA patterns. Of these, 46.62% were malignant. No cancer was found in any of the ATA benign pattern nodules. The Bayesian analysis post-test probability for cancer in each classification was: (1) Very-low suspicion, 0.85%; (2) Low, 2.6%; (3) Intermediate, 6.7%; and (4) High, 40.9%. The net benefit (NB), expressed as avoided interventions, indicated that the highest capacity to avoid unnecessary fine needle aspiration biopsy (FNAB) in the patterns that we studied was 42, 31, 35, and 43 of every 100 FNABs. The NB calculation for a probability threshold of 11% for each of the ATA suspicion patterns studied is less than that of performing FNAB on all nodules.</p><p><strong>Conclusion: </strong>These three types of analysis have shown that only the ATA high-suspicion diagnostic pattern is clinically useful, in which case, FNAB should be performed. However, the curve decision analysis has demonstrated that using the ATA US risk patterns to decide which patients need FNAB does not provide a greater benefit than performing FNAB on all thyroid nodules. Therefore, it is likely that a better way to approach the assessment of thyroid nodules would be to perform FNAB on all non-cystic nodules, as the present analysis has shown the ATA risk patterns do not provide an adequate clinical decision-making framework.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"148-163"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/18/06/WJM-12-148.PMC9157633.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40027191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature and mechanism of immune boosting by Ayurvedic medicine: A systematic review of randomized controlled trials.","authors":"B N Vallish, Dimple Dang, Amit Dang","doi":"10.5662/wjm.v12.i3.132","DOIUrl":"10.5662/wjm.v12.i3.132","url":null,"abstract":"<p><strong>Background: </strong>Many Ayurvedic preparations are claimed to have immune-boosting properties, as suggested in various published randomized clinical trials (RCTs).</p><p><strong>Aim: </strong>To compile evidence on the nature and mechanism of immune system enhancement by Ayurvedic preparations in healthy and sick individuals.</p><p><strong>Methods: </strong>After prospectively registering study protocol with PROSPERO, we searched PubMed, DOAJ, Google Scholar, three dedicated Ayurveda research portals, two specialty Ayurveda journals, and reference lists for relevant records published until February 6, 2021 using appropriate search strategies. Baseline features and data pertaining to the nature and mechanism of immune system function were extracted from all eligible records. Methodological quality was assessed using the Cochrane RoB-2 tool.</p><p><strong>Results: </strong>Of 12554 articles screened, 19 studies reporting 20 RCTs (17 parallel group design, three crossover design) with 1661 unique patients were included; 11/19 studies had Indian first authors. Healthy population was included in nine studies, of which one study included pregnant women and two included pediatric population; remaining studies included patients with different health conditions, including one study with coronavirus disease 2019 patients. A total of 21 Ayurvedic interventions were studied, out of which five were composite mixtures. The predominant route of administration was oral; dose and frequency of administration of the intervention varied across the studies. The results reported with five RCTs exploring five Ayurvedic interventions were incomplete, ambiguous, or confusing. Of the remaining 16 interventions, indirect evidence of immune enhancement was reported with four interventions, while lack of the same was reported with two interventions. Enhancement of T helper cells and natural killer cells was reported with three and four interventions, respectively, while the pooled results did not clearly point toward enhancement of other components of the immune system, including cytotoxic T cells, B lymphocytes, immunoglobulins, cytokines, complement components, leucocyte counts, and other components. Nine of the 20 RCTs had a high risk of bias, and the remaining 11 RCTs had some concerns according to RoB-2.</p><p><strong>Conclusion: </strong>Various Ayurvedic preparations appear to enhance the immune system, particularly <i>via</i> enhancements in natural killer cells and T helper cells.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"132-147"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/31/b5/WJM-12-132.PMC9157632.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40026717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and serology methods in the diagnosis of COVID-19: An overview.","authors":"Marcel Silva Luz,&nbsp;Ronaldo Teixeira da Silva Júnior,&nbsp;Gabriella Almeida Santos de Santana,&nbsp;Gabriela Santos Rodrigues,&nbsp;Henrique de Lima Crivellaro,&nbsp;Mariana Santos Calmon,&nbsp;Clara Faria Souza Mendes Dos Santos,&nbsp;Luis Guilherme de Oliveira Silva,&nbsp;Qesya Rodrigues Ferreira,&nbsp;Guilherme Rabelo Mota,&nbsp;Heloísa Heim,&nbsp;Filipe Antônio França da Silva,&nbsp;Breno Bittencourt de Brito,&nbsp;Fabrício Freire de Melo","doi":"10.5662/wjm.v12.i3.83","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.83","url":null,"abstract":"<p><p>Coronavirus disease-19 (COVID-19) has become a pandemic, being a global health concern since December 2019 when the first cases were reported. Severe acute respiratory syndrome coronavirus 2, the COVID-19 causal agent, is a β-coronavirus that has on its surface the spike protein, which helps in its virulence and pathogenicity towards the host. Thus, effective and applicable diagnostic methods to this disease come as an important tool for the management of the patients. The use of the molecular technique PCR, which allows the detection of the viral RNA through nasopharyngeal swabs, is considered the gold standard test for the diagnosis of COVID-19. Moreover, serological methods, such as enzyme-linked immunosorbent assays and rapid tests, are able to detect severe acute respiratory syndrome coronavirus 2-specific immunoglobulin A, immunoglobulin M, and immunoglobulin G in positive patients, being important alternative techniques for the diagnostic establishment and epidemiological surveillance. On the other hand, reverse transcription loop-mediated isothermal amplification also proved to be a useful diagnostic method for the infection, mainly because it does not require a sophisticated laboratory apparatus and has similar specificity and sensitivity to PCR. Complementarily, imaging exams provide findings of typical pneumonia, such as the ground-glass opacity radiological pattern on chest computed tomography scanning, which along with laboratory tests assist in the diagnosis of COVID-19.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"83-91"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/29/96/WJM-12-83.PMC9157626.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40027192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Participant attrition and perinatal outcomes in prenatal vitamin D-supplemented gestational diabetes mellitus patients in Asia: A meta-analysis.","authors":"Sumanta Saha,&nbsp;Sujata Saha","doi":"10.5662/wjm.v12.i3.164","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.164","url":null,"abstract":"<p><strong>Background: </strong>The role of vitamin D supplementation in gestational diabetes mellitus (GDM) patients is unclear.</p><p><strong>Aim: </strong>To determine the burden and risk of post-randomization GDM patient attrition from vitamin D-supplemented arms of randomized controlled trials (RCTs). The auxiliary aim was to compare the effects of nutritional supplements on their fasting blood glucose (FPG) levels and perinatal outcomes.</p><p><strong>Methods: </strong>RCTs were searched in the PubMed, Embase, and Scopus databases. Random-effect prevalence and pairwise meta-analysis were performed for the primary objective. The auxiliary aim was to compare the effects of nutritional supplements on their fasting blood glucose (FPG) levels and perinatal outcomes. Fixed-effect network meta-analyses were undertaken for the secondary goals. All analyses were performed using Stata software, and statistical significance was determined at <i>P</i> < 0.05.</p><p><strong>Results: </strong>Thirteen RCTs from Iran and China were reviewed. The participant attrition burden in vitamin D recipients was 6% [95% confidence interval (CI): 0.03, 0.10], and its risk did not vary from non-recipients. Vitamin D and calcium co-supplementation reduced the cesarean section incidence in GDM patients [risk ratio (RR): 0.37; 95%CI: 0.18, 0.74]. The hyperbilirubinemia or hospitalization risk in their newborns decreased with vitamin D supplementation (RR: 0.47; 95%CI: 0.27, 0.83) and co-supplementation with calcium (RR: 0.35; 95%CI: 0.16, 0.77) or omega-3 fatty acids (RR: 0.25; 95%CI: 0.08, 0.77). Vitamin D and probiotics co-supplementation decreased newborn hyperbilirubinemia risk (RR: 0.28; 95%CI: 0.09, 0.91). FPG levels and macrosomia risk did not vary across interventions.</p><p><strong>Conclusion: </strong>In RCTs, vitamin D supplementation or co-supplementation in GDM patients showed a low participant attrition burden and low risk of cesarean section, newborn hyperbilirubinemia, and newborn hospitalization.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"164-178"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/8a/WJM-12-164.PMC9157628.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40026719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-use duodenoscopes for the prevention of endoscopic retrograde cholangiopancreatography -related cross-infection - from bench studies to clinical evidence.","authors":"Andrea Lisotti,&nbsp;Pietro Fusaroli,&nbsp;Bertrand Napoleon,&nbsp;Anna Cominardi,&nbsp;Rocco Maurizio Zagari","doi":"10.5662/wjm.v12.i3.122","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.122","url":null,"abstract":"<p><strong>Background: </strong>Several strategies have been implemented to reduce or abolish the life-threatening risk of endoscopic retrograde cholangiopancreatography (ERCP)-related multidrug-resistant infections due to duodenoscopes contaminations; among those strategies, serial microbiologic tests, thorough reprocessing schedules, and use of removable scope cap have been adopted, but the potential cross-infection risk was not eliminated.</p><p><strong>Aim: </strong>To review available evidence in the field of single-use duodenoscopes (SUD) use for ERCP.</p><p><strong>Methods: </strong>An overview on ongoing clinical studies was also performed to delineate which data will become available in the next future.</p><p><strong>Results: </strong>One bench comparative study and four clinical trials performed with EXALT model-D (Boston Scientific Corp., United States) have been identified. Of them, one is a randomized controlled trial, while the other three studies are prospective single-arm, cross-over studies. Pooled technical success rate (4 studies, 368 patients) was 92.9% [95% confidence interval (CI): 89.9-95.5; <i>I</i> ²: 11.8%]. Pooled serious adverse event (4 studies, 381 patients) rate was 5.9% [3.7%-8.5%; <i>I</i> ²: 0.0%].</p><p><strong>Conclusion: </strong>Although few clinical trials are available, evidence is concordant in identifying an absolute feasibility and safety and feasibility for SUD use for ERCP. The expertise and quality of evidence in this field are going to be improved by further large clinical trials;data on cost-effectiveness and environmental impact will be needed for a worldwide spread of SUD use for ERCP.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"122-131"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/ff/WJM-12-122.PMC9157629.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40027194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Severe acute respiratory syndrome coronavirus 2 pandemic and surgical diseases: Correspondence.","authors":"Pathum Sookaromdee,&nbsp;Viroj Wiwanitkit","doi":"10.5662/wjm.v12.i3.191","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.191","url":null,"abstract":"<p><p>This letter to editor discussing on the publication on severe acute respiratory syndrome coronavirus 2 pandemic and surgical diseases. Concerns on procedures are raised and discussed.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"191-192"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8f/aa/WJM-12-191.PMC9157631.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40026713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence of occult hepatitis C virus: A systematic review and meta-analysis.","authors":"Donatien Serge Mbaga,&nbsp;Sebastien Kenmoe,&nbsp;Jacky Njiki Bikoï,&nbsp;Guy Roussel Takuissu,&nbsp;Marie Amougou-Atsama,&nbsp;Etienne Atenguena Okobalemba,&nbsp;Jean Thierry Ebogo-Belobo,&nbsp;Arnol Bowo-Ngandji,&nbsp;Martin Gael Oyono,&nbsp;Jeannette Nina Magoudjou-Pekam,&nbsp;Ginette Irma Kame-Ngasse,&nbsp;Alex Durand Nka,&nbsp;Alfloditte Flore Feudjio,&nbsp;Cromwel Zemnou-Tepap,&nbsp;Elie Adamou Velhima,&nbsp;Juliette Laure Ndzie Ondigui,&nbsp;Rachel Audrey Nayang-Mundo,&nbsp;Sabine Aimee Touangnou-Chamda,&nbsp;Yrene Kamtchueng Takeu,&nbsp;Jean Bosco Taya-Fokou,&nbsp;Chris Andre Mbongue Mikangue,&nbsp;Raoul Kenfack-Momo,&nbsp;Cyprien Kengne-Ndé,&nbsp;Carole Stephanie Sake,&nbsp;Seraphine Nkie Esemu,&nbsp;Richard Njouom,&nbsp;Lucy Ndip,&nbsp;Sara Honorine Riwom Essama","doi":"10.5662/wjm.v12.i3.179","DOIUrl":"https://doi.org/10.5662/wjm.v12.i3.179","url":null,"abstract":"<p><strong>Background: </strong>Occult hepatitis C infection (OCI) is characterized by the presence of hepatitis C virus (HCV) RNA in the liver, peripheral blood mononuclear cells (PBMC) and/or ultracentrifuged serum in the absence of detectable HCV-RNA in serum. OCI has been described in several categories of populations including hemodialysis patients, patients with a sustained virological response, immunocompromised individuals, patients with abnormal hepatic function, and apparently healthy subjects.</p><p><strong>Aim: </strong>To highlight the global prevalence of OCI.</p><p><strong>Methods: </strong>We performed a systematic and comprehensive literature search in the following 4 electronic databases PubMed, EMBASE, Global Index Medicus, and Web of Science up to 6th May 2021 to retrieve relevant studies published in the field. Included studies were unrestricted population categories with known RNA status in serum, PBMC, liver tissue and/or ultracentrifuged serum. Data were extracted independently by each author and the Hoy <i>et al</i> tool was used to assess the quality of the included studies. We used the random-effect meta-analysis model to estimate the proportions of OCI and their 95% confidence intervals (95%CI). The Cochran's <i>Q</i>-test and the <i>I</i> ² test statistics were used to assess heterogeneity between studies. Funnel plot and Egger test were used to examine publication bias. R software version 4.1.0 was used for all analyses.</p><p><strong>Results: </strong>The electronic search resulted in 3950 articles. We obtained 102 prevalence data from 85 included studies. The pooled prevalence of seronegative OCI was estimated to be 9.61% (95%CI: 6.84-12.73) with substantial heterogeneity [<i>I</i>² = 94.7% (95%CI: 93.8%-95.4%), <i>P</i> < 0.0001]. Seropositive OCI prevalence was estimated to be 13.39% (95%CI: 7.85-19.99) with substantial heterogeneity [<i>I</i> ² = 93.0% (90.8%-94.7%)]. Higher seronegative OCI prevalence was found in Southern Europe and Northern Africa, and in patients with abnormal liver function, hematological disorders, and kidney diseases. Higher seropositive OCI prevalence was found in Southern Europe, Northern America, and Northern Africa.</p><p><strong>Conclusion: </strong>In conclusion, in the present study, it appears that the burden of OCI is high and variable across the different regions and population categories. Further studies on OCI are needed to assess the transmissibility, clinical significance, long-term outcome, and need for treatment.</p>","PeriodicalId":23729,"journal":{"name":"World journal of methodology","volume":"12 3","pages":"179-190"},"PeriodicalIF":0.0,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/8c/WJM-12-179.PMC9157636.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40026716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}