VirusDisease最新文献

{"title":"Identification of nine putative novel members of plant-infecting alphaflexiviruses in public domain plant transcriptomes.","authors":"B Sravani, V Kavi Sidharthan, Vijayprakash Reddy","doi":"10.1007/s13337-024-00898-3","DOIUrl":"10.1007/s13337-024-00898-3","url":null,"abstract":"<p><p>Plant-infecting alphaflexiviruses cause moderate to severe diseases in economically important crops worldwide. In the present study, we identified nine putative novel alphaflexiviruses in nine plant species by exploring the publicly available plant transcriptome data in Sequence Read Archive (SRA) database. Coding-complete genomes of all the identified viruses were recovered and contained five to six open reading frames (ORFs). ORFs 1-5 encode replicase (Rep), triple gene block (TGB) proteins 1-3 and coat protein (CP), respectively. The additional ORF6, identified in two viruses, encoded the nucleic acid-binding (NB) protein or a protein with no significant similarity to known viral sequences. Genome organization of the first alphaflexivirus identified in a gymnospermic host (black pine potex-like virus 1-BlpPV1) slightly differed from that of known alphaflexiviruses and formed a distinct sub-clade in phylogenetic analysis. Thus, BlpPV1 can represent a novel taxon within the family <i>Alphaflexiviridae</i>. Based on phylogeny, sequence similarity to known members and sequence-based species demarcation criteria, six other identified viruses were tentatively assigned to the genera <i>Potexvirus</i> (4), <i>Lolavirus</i> (1) and <i>Mandarivirus</i> (1), while the two lola-like viruses may potentially represent a new genus. Further studies are needed to understand the biology and geographical spread of identified novel viruses.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13337-024-00898-3.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 4","pages":"630-636"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-emerging <i>Chandipura</i> <i>vesiculovirus</i>: A cause of concern for global health.","authors":"Abhishek Padhi, Ashwini Agarwal, Praggya Mishra, Ekta Gupta, Swatantra Kumar, C D S Katoch, Shailendra K Saxena","doi":"10.1007/s13337-024-00896-5","DOIUrl":"10.1007/s13337-024-00896-5","url":null,"abstract":"<p><p><i>Chandipura vesiculovirus</i> (CHPV) is an emerging neurotropic virus primarily affecting children and causing acute encephalitis syndrome (AES) in India. The virus, transmitted mainly by sand flies, has led to multiple outbreaks with high mortality rates, particularly in rural and resource-limited settings. CHPV infection is characterized by rapid disease progression, with symptoms ranging from fever and seizures to coma and death, often within 24 to 48 h of onset. The current management of CHPV is limited to supportive care due to the lack of specific antiviral therapies. Diagnosis relies on laboratory methods such as RT-PCR, serology, and immunofluorescence, though these face challenges due to the rapid progression of the disease and the need for timely sample collection and analysis. Prevention strategies are focused on vector control through insecticide use and public health interventions, including community education and early detection programs. Despite some progress in understanding CHPV, significant research gaps remain, particularly in developing effective antiviral treatments and vaccines, understanding transmission dynamics, and improving diagnostic capabilities. The potential for the virus to spread globally due to factors like climate change and increased human movement underscores the need for international collaboration in surveillance and response efforts. Strengthening public health infrastructure, enhancing vector control measures, and fostering global partnerships are crucial steps toward mitigating the impact of CHPV and preventing future outbreaks. Continued research and proactive public health strategies are essential to protect vulnerable populations and control the spread of this potentially deadly virus.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"385-399"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic evolution of peste des petits ruminants virus in sheep and goat hosts across India reveals the swift surge of F gene.","authors":"Varsha Ramesh, Kuralayanapalya P Suresh, Shijili Mambully, Swati Rani, Rakshit Ojha, Kirubakaran V Kumar, Vinayagamurthy Balamurugan","doi":"10.1007/s13337-024-00890-x","DOIUrl":"10.1007/s13337-024-00890-x","url":null,"abstract":"<p><p>Peste des petits ruminants (PPR), an acute febrile viral disease impacting goats and sheep flocks, manifests with pyrexia, mucopurulent nasal and ocular discharges, necrotizing and erosive stomatitis, pneumonia, and enteritis. The disease-instigating agent, PPR virus, pertains to the <i>Morbillivirus caprinae</i> genus in the <i>Paramyxoviridae</i> family. The endemic presence of PPR in India results in notable economic losses due to heightened mortality and morbidity in infected animals. Understanding viral pathogen evolution is pivotal for delineating their emergence in diverse environments. This study explores the molecular evolutionary patterns of PPRV, concentrating on the N and F structural genes isolated from Indian sheep and goats. Analyzing evolutionary rate, phylogenetics, selection pressure, and codon usage bias, we determined the time to the most recent common ancestor (tMRCA) as 1984, 1973, 2000, and 2004 for goat and sheep's N and F genes, respectively, with evolutionary rates ranging from 2.859 x 10³ to 4.995 x 10⁴. The F-gene is found to exhibit a faster evolution than the N-gene, indicating apparent virus transmission across the regions of India, as supported by phylogenetic analysis. Codon usage bias examination, incorporating nucleotide composition and various plots (effective number of codon plot, parity plot, neutrality plot), suggests the evolution in India influenced by both natural selection and mutational pressure, resulting in alterations in the virus's codon bias. The integrated analysis underscores the significant role of selection pressures, implying PPRV's co-evolution and adaptations influenced by various genes. Insights from this study can guide effective disease control and vaccine development, aiding in managing PPR outbreaks in India and beyond.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"505-519"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of occult hepatitis C virus infection (OCI) among hemodialysis patients; a cross-sectional study from Lorestan Province, Western Iran.","authors":"Banafsheh Hasanvand, Habibollah Mirzaei, Babak Hadian, Parsa Sasaei, Sara Garavand, Hemanta Adhikary, Sayyad Khanizadeh","doi":"10.1007/s13337-024-00881-y","DOIUrl":"10.1007/s13337-024-00881-y","url":null,"abstract":"<p><p>Patients with chronic kidney disorders, such as hemodialysis, are at a higher risk of infection with hepatitis viruses than the other population due to high nosocomial transmission by the dialysis units. The incidence of occult <i>HCV</i> infection (OCI) among chronic hemodialysis (CHD) remains controversial and the real burden of <i>HCV</i> in this population may be affected by the rate of OCI. This study aimed to evaluate the prevalence of OCI among patients with CHD from Lorestan province, Western Iran. In this cross-sectional study, whole blood samples were collected from 122 patients with CHD. Subsequently, anti-<i>HCV</i> antibody and <i>HCV</i>-RNA were assessed in serum/peripheral blood mononuclear cells (PBMCs) using Enzyme-Linked Immunosorbent Assay and Real-Time PCR technique, respectively. Out of the 122 patients, 61.15% were male and 38.8% were female. Regarding <i>HCV</i> results, out of the 122 studied patients, 4 cases (3.3%) were positive for anti-HCV IgM Ab and 3 patients (2.47%) for anti-<i>HCV</i> IgG Ab in their serum. Moreover, none of the 122 patients were positive for <i>HCV</i>-RNA in serum samples, while in PBMC samples, two patients (1.6%) tested positive for <i>HCV</i>-RNA, of which one patient was anti-<i>HCV</i> IgG Ab positive. Furthermore, the prevalence of OCI was correlated with the history of blood transfusion and serum level of transaminases (<i>P</i> = 0.012). The results of the current study suggest that there is a potential risk of occult <i>HCV</i> infection among patients undergoing hemodialysis. Therefore, it is necessary to use appropriate molecular techniques for early diagnosis and treatment of these patients.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"478-483"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and phylogenetic analysis of bovine herpesvirus 4 isolated from aborted bovine foetuses in Türkiye.","authors":"Mustafa Emin Oz, Muge Dogan, Sezer Akbaba, Yunus Cakici","doi":"10.1007/s13337-024-00886-7","DOIUrl":"10.1007/s13337-024-00886-7","url":null,"abstract":"<p><p>Bovine herpesvirus 4 (BoHV-4) is responsible for reproductive disorders and abortions in cattle. The aim of this study was to investigate the potential role of BoHV-4 in abortions in dairy cows when the possible aetiological cause could not be determined in Türkiye. The study also provided the molecular characterization of BoHV-4 strains based on the gB gene. In this study, foetuses (<i>n</i> = 188) and serum (<i>n</i> = 50) were retrospectively used for PCR and indirect ELISA assays, respectively. In addition, foetuses with BoHV-4 were tested for the isolation of the BoHV-4 strain in MDBK cell culture. The BoHV-4 gB gene was detected in the brain, liver, lung, kidney, and spleen tissues of three foetuses out of 188 aborted foetuses. The BoHV-4 strain was isolated from the kidney of one foetus in MDBK cell culture. The three aborted foetuses were from different cows in the same herd in 2021. On this dairy farm, the BoHV-4 seroprevalence rate in cattle with a history of abortion was 96% (48/50). Phylogenetic analysis revealed that the detected strains belong to genotype II. The present study shows that the impact of BoHV-4 on abortions in cattle should not be ignored. It can therefore be informative to focus on BoHV-4 in routine laboratory diagnostics for abortions without a proven aetiology or in cattle herds that have previously been infected with BoHV-4.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"496-504"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of begomovirus and DNA satellites associated with mosaic and leaf curl disease of Jamaica cherry (<i>Muntingia calabura)</i> in India: Uncovering a new host for chilli leaf curl India virus.","authors":"K S Uday Durga Prasad, S S Kavya, Kopparthi Amrutha Valli Sindhura, Mantesh Muttappagol, R Kruthika, C R Jahir Basha, K S Shankarappa, V Venkataravanappa, C N Lakshminarayana Reddy","doi":"10.1007/s13337-024-00891-w","DOIUrl":"10.1007/s13337-024-00891-w","url":null,"abstract":"<p><p>Begomoviruses, member of the <i>Geminiviridae</i> family, are responsible for significant economic losses in crops worldwide. Chilli leaf curl India virus (ChiLCINV) is a well-known begomovirus that causes leaf curl disease, primarily affecting plants in the <i>Solanaceae</i> family. In this study, sample from a Jamaica cherry (<i>Muntingia calabura</i>) tree showing typical begomovirus symptoms of mosaic and leaf curling was collected from Nagavara village in the Bengaluru Rural district of Karnataka State, India. The collected sample was designated as the MUT-1 isolate. The association of the begomovirus (DNA-A) and betasatellites with the sample was confirmed by PCR using begomovirus-specific primers, resulting in the expected amplicons of approximately 1.2 kb and 1.3 kb, respectively. No amplification was obtained for DNA-B and alphasatellite specific primers. The complete genome sequence of DNA-A of begomovirus isolate MUT-1 was obtained through rolling circle amplification and compared with other begomoviruses using Sequence Demarcation Tool which revealed that, DNA-A of MUT-1 isolate, (Acc.No. PP475538) showed maximum nucleotide (nt) identity of 98.7-99.4% with chilli leaf curl India virus. Further, sequence of betasattelite (Acc.No. PP493212) of this isolate shared maximum nt identity of 86.5-100% with tomato leaf curl Bangladesh betasatellite (ToLCBDB). Recombination and GC plot analysis showed that the presence of two and three intraspecific recombination event in DNA-A and betasatellite genomic regions, respectively and are derived from the previously reported begomoviruses. This study presents one more evidence of expanding host range for begomoviruses and first record of begomovirus associated with mosaic and leaf curl disease of Jamaica cherry (<i>M. calabura</i>) from India.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13337-024-00891-w.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"484-495"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in understanding plant-pathogen interactions: insights from tomato as a model system.","authors":"Sekinat Okikiola Azeez, Seyi Ebun Adeboye","doi":"10.1007/s13337-024-00889-4","DOIUrl":"10.1007/s13337-024-00889-4","url":null,"abstract":"<p><p>The impact of plant diseases coupled with climate change on agriculture worldwide cannot be overemphasized from negative effects on crop yield as well as economy to food insecurity. The model plants are essential for understanding the intricacies of plant-pathogen interactions. One of such plants is the tomato (<i>Solanum lycopersicum</i> L.). Researchers hope to increase tomato productivity and boost its resilience to pathogen attacks by utilizing OMICS and biotechnological methods. With an emphasis on tomato viral infections, this review summarizes significant discoveries and developments from earlier research. The analysis elucidates ongoing efforts to advance plant pathology by exploring the implications for sustainability and tomato production.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"537-552"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and full-length genome characterization of a novel mitovirus hosted by the truffle species <i>Tuber rufum</i>.","authors":"Ilgaz Akata, Gulce Edis, Eda Kumru, Ergin Sahin","doi":"10.1007/s13337-024-00887-6","DOIUrl":"10.1007/s13337-024-00887-6","url":null,"abstract":"<p><p>Studying the diversity of viruses found in uncultivated fungi, including those forming mycorrhizal relationships, is essential. It's equally important to explore viral communities in fungi that cause plant diseases or are economically significant. This dual approach helps us grasp the full scope of mycovirus diversity and evolution. Mycorrhizal fungi, in particular, host a wide range of viruses, shedding light on viral diversity and evolution. In this study, we present the discovery and complete genome characterization of a novel mitovirus infecting the hypogeous mycorrhizal fungus <i>Tuber rufum</i>. This virus, denominated \"Tuber rufum mitovirus 1\" (TrMV1) has a genome size of 2864 nucleotides with a G + C content of 37.53%. It contains a single open reading frame (ORF) responsible for encoding RNA dependent RNA polymerase (RdRp). Comparative analysis using BLASTp reveals that the protein encoded by TrMV1 shares significant sequence similarities with those found in the <i>Triamitovirus</i> genus. Specifically, TrMV1 shows the closest resemblance (43.35% identity) to Sopawar virus, a mitovirus previously detected in soil environments through metatranscriptomic analyses. Phylogenetic examination categorizes TrMV1 as a member of the <i>Triamitovirus</i> genus within the <i>Mitoviridae</i> family. This finding marks the first identification of a mitovirus within the hypogeous mycorrhizal fungus <i>Tuber rufum</i>. The discovery of TrMV1 expands our knowledge of <i>Mitoviridae</i> family diversity and evolution, contributing to the growing repository of mycovirus sequences.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13337-024-00887-6.</p>","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"35 3","pages":"531-536"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11502633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization, phylogenetic and variation analyses of SARS-CoV-2 strains in India","authors":"Meghna Banerjee, Dipjyoti Chakraborty, Arindom Chakraborty","doi":"10.1007/s13337-024-00878-7","DOIUrl":"https://doi.org/10.1007/s13337-024-00878-7","url":null,"abstract":"","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"44 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141922618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning-based gene expression biomarkers to distinguish Zika and Dengue virus infections: implications for diagnosis","authors":"Ayesha Zeba, Aruna Rajalingam, Kanagaraj Sekar, Anjali Ganjiwale","doi":"10.1007/s13337-024-00885-8","DOIUrl":"https://doi.org/10.1007/s13337-024-00885-8","url":null,"abstract":"","PeriodicalId":23708,"journal":{"name":"VirusDisease","volume":"31 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141928593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}