Vector borne and zoonotic diseases最新文献

{"title":"Meteorological Effects on the Abundance of West Nile Virus Mosquito Vectors in Two Communities Located on the Texas-Mexico Border in the Northern Chihuahuan Desert.","authors":"Adam J Vera, Albert G Soliz, Karen R Vera, Oscar J Ortega, Chitra B Karki, Xiaogang Su, Philip Lavretsky, Antonio de la Mora-Covarrubia, Douglas M Watts","doi":"10.1177/15303667251382345","DOIUrl":"https://doi.org/10.1177/15303667251382345","url":null,"abstract":"<p><p><b><i>Background:</i></b> Mosquitoes in the genus <i>Culex</i> are vectors of multiple arboviruses, including West Nile virus, St. Louis Encephalitis virus, and Western equine encephalitis virus. In the United States, West Nile (WN) is the leading cause of mosquito-borne diseases, including the El Paso, Texas, and surrounding communities. While <i>Cx. quinquefasciatus</i>, <i>Cx. tarsalis</i>, and <i>Cx. erythrothorax</i> are well documented in the El Paso region, the meteorological effects on the overall abundance and virus transmission risk are not well understood. Our study objective was to estimate temporal population abundance in two urban communities: Sparks, El Paso, Texas, and Anapra, Ciudad Juárez, Chihuahua, Mexico. <b><i>Materials and Methods:</i></b> Population abundance was estimated through collections of <i>Cx. quinquefasciatus</i>, <i>Cx. tarsalis</i>, and <i>Cx. erythrothorax</i> from June to December across years 2016-2018 using confirmed human case gravid traps. Mosquitoes were collected inside and outside 108 and 101 participating homes in Sparks and Anapra, respectively. <b><i>Results:</i></b> Generally, the results indicated that the abundance of these mosquito species was highest in June, followed by a slight decrease and then an increase in abundance during August, followed by a slight decrease until their near absence in December with colder winter season. The high abundance early in the year could be attributed to overwintering behavior which allowed these species to become dormant and/or diapause until ideal favorable environmental conditions return year after year during the spring season. <b><i>Conclusion:</i></b> Interestingly, the overall abundance of <i>Cx. spp.</i> was greater in Anapra, where no WN human cases have been reported. Our study provided a better understanding of the seasonal abundance and risk of arbovirus transmission by these medically important mosquitoes and the need to implement vector control measures early in the year.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of Camel Brucellosis and Its Impact on Human Health in Endemic Regions.","authors":"Ahmed Abdel-Rady, Hanin Ashraf Abu-Seida, Ashraf M Abu-Seida","doi":"10.1177/15303667251380273","DOIUrl":"https://doi.org/10.1177/15303667251380273","url":null,"abstract":"<p><p><b><i>Background:</i></b> Camel brucellosis is a zoonotic disease caused by the genus <i>Brucella</i> and has significant economic and public health effects worldwide, particularly in areas where camels play a significant role in the pastoral economy. This review describes the epidemiological aspects of camel brucellosis and its impact on human health. <b><i>Methods:</i></b> A comprehensive literature search was performed using PubMed, Web of Science, Scopus, and Google Scholar databases to locate studies on camel brucellosis for recognizing its epidemiological aspects and impact on human health. The search included 274 documents. <b><i>Results:</i></b> The disease results in reduced milk output, reproductive failures, arthritis, and financial losses for farmers. The seroprevalence of camel brucellosis across the camel-rearing countries is alarming and ranges between 0.4 and 37.5%. Sudan and the Kingdom of Saudi Arabia are the most affected countries with camel brucellosis. Three species were isolated from diseased camels, including <i>B. abortus</i>, <i>B. melitensis</i>, and <i>B. suis.</i> The potential risk factors of camel brucellosis include age, body condition, gender, physiological status and breed of the camel, herd size, contact with cattle and small ruminants, management, history of abortion, locality, and season. Accurate diagnosis of camel brucellosis is essential for effective management of the disease. Serodiagnosis is still one of the most used techniques for identifying the affected animals. <b><i>Conclusions:</i></b> A one health strategy is required from the governments, animal health professionals, and other stakeholders to combat the disease. This strategy includes enhancing veterinary care, increasing public and herders awareness toward the zoonotic importance of the disease as well as value of biosecurity procedures, and putting in place efficient immunization programs aimed at high-risk groups. Due to the socioeconomic impact of camel brucellosis, it is crucial to understand its epidemiology and potential risk factors to implement effective control strategies.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145087480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arboviral Diseases in a Changing World: Evolutionary Dynamics, Host-Vector Interactions, and Novel Control Strategies.","authors":"Ebrahim Abbasi","doi":"10.1177/15303667251376450","DOIUrl":"https://doi.org/10.1177/15303667251376450","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Arboviral diseases, transmitted by hematophagous arthropods such as mosquitoes and ticks, represent an escalating global public health challenge. The resurgence and geographic spread of arboviruses particularly dengue virus, Zika virus, chikungunya virus (CHIKV), and West Nile virus are closely linked to environmental change, urbanization, and increased human mobility. Understanding their evolutionary mechanisms, host-vector interactions, and emerging control strategies is critical to effective disease mitigation. <b><i>Materials and Methods:</i></b> This systematic review employed a comprehensive multidatabase search (PubMed, Scopus, Web of Science, Google Scholar) from 2000 to 2025 using MeSH terms and Boolean logic to identify studies on arbovirus evolution, transmission, and control. From 16,320 initial records, 12 high-quality, peer-reviewed studies met the final inclusion criteria based on relevance, methodology, and publication standards. The review followed PRISMA guidelines and adopted an integrative analytical framework, including genomic analysis, meta-epidemiological synthesis, and predictive modeling. <b><i>Results:</i></b> The review highlights that arboviruses possess high genomic plasticity, enabling rapid adaptation through mutations (<i>e.g.,</i> CHIKV A226V), recombination, and immune evasion. Key molecular mechanisms include subversion of RNA interference (RNAi) and Toll/IMD pathways, and saliva-assisted transmission in vectors. Environmental and anthropogenic driver's climate change, urban sprawl, and globalization are expanding arbovirus endemicity into new regions. Novel control strategies such as CRISPR gene drives, <i>Wolbachia</i>-based interventions, and RNAi antivirals offer promising alternatives to conventional vector control, with mRNA vaccine platforms showing significant potential. <b><i>Discussion:</i></b> The findings emphasize the importance of a multidisciplinary approach integrating virology, vector biology, synthetic biology, and environmental modeling. Real-time genomic surveillance, predictive analytics, and eco-adaptive vector control strategies are essential for proactive response. However, ethical, ecological, and regulatory concerns around gene editing and microbial interventions warrant careful consideration. The evolving interplay between virus, vector, host, and environment necessitates dynamic public health strategies and sustained international collaboration.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predisposing Factors, Control Strategies, and Antiviral Therapy of Mpox Virus as a Potential Zoonotic Agent.","authors":"Elham Zarenezhad, Mahrokh Marzi, Fattma A Ali, Ahmed Shayaa Kareem, Mehdi Rezaian, Abdolmajid Ghasemian","doi":"10.1177/15303667251378931","DOIUrl":"https://doi.org/10.1177/15303667251378931","url":null,"abstract":"<p><p><b><i>Background:</i></b> Monkeypox (Mpox) virus is a virulent zoonotic agent and a member of <i>Poxviridae</i> family. Owing to environmental, social and behavioral factors, and lack of approved vaccines or antiviral agents, the eradication of Mpox has not been successful until today. <b><i>Material and methods:</i></b> This study overviewed published data to assess predisposing factors, control strategies and antiviral therapy of mpox virus. <b><i>Results:</i></b> The recent Mpox outbreak occurred due to human behavioral factors with a cumulative total of 91,123 laboratory-confirmed cases, including 157 deaths during March 2022-September 2023. Viral immunoregulatory proteins, such as vaccinia complement control protein, inflammation modulatory protein, smallpox inhibitor of complement enzymes, chemokine binding protein, and vaccinia virus growth factor, suppress efficient responses. Host genetic variations in IL1RN (interleukin 1 Receptor Antagonist, rs452204) and IL12RB2 (rs3790567) genes and interferon-α deficiency predispose the host to more severe Mpox infection. <b><i>Conclusion:</i></b> Combination therapy and drug repositioning include promising approaches for the efficient antiviral therapy. The worldwide collaboration is required to enhance outbreak preparedness and infection control.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145070568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"West Nile Virus and Usutu Virus Neutralizing Antibodies Found in Dutch Rodent Species.","authors":"Nnomzie C Atama, Beatriz B Martin, Mees G van Horssen, Felicity D Chandler, Emily L Pascoe, Marieke P de Cock, Miriam Maas, Cora M Holicki, Helen J Esser, Constantianus J M Koenraadt, Marion P G Koopmans, Maarten Schrama, Reina S Sikkema","doi":"10.1177/15303667251380275","DOIUrl":"https://doi.org/10.1177/15303667251380275","url":null,"abstract":"<p><p><b><i>Background:</i></b> In the Netherlands, Usutu virus (USUV) is endemic in birds, and recently West Nile virus (WNV) was also detected in birds, mosquitoes and humans. Here we investigated the possible role of rodents in the viruses' transmission ecology. <b><i>Materials and Methods:</i></b> We sampled rodents at six locations including sites where WNV had been previously detected. Brains (<i>n</i> = 668), oral swabs (<i>n</i> = 282), and ticks (<i>n</i> = 91) collected from rodents were tested for arboviruses via Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Also, sera from 118 rodents were tested for WNV- and USUV-antibodies. <b><i>Results and Conclusion:</i></b> Brain samples, swabs, and ticks tested negative for viral RNA. However, 2.5% (3/118; two wood mice, one field vole) of rodents had WNV-neutralizing antibodies (WNV-NAbs). USUV-NAbs were detected in a wood mouse. Two bank voles had NAbs against both viruses. The WNV and USUV antibody-positive rodents were found at locations with previous WNV and USUV circulations, suggesting that rodents may be involved in the ecology of WNV and USUV.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145056029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Overview of Destroying SARS-CoV-2 and Proof of the Relationship Between Climate Changes and Airborne Dust on SARS-CoV-2 Prevalence.","authors":"Mohamed Madani, Hasi Rani Barai, Dalal Mohamed Alshangiti, Sheikha Alkhursani, Samera Ali Al-Gahtany, Mohamed Mohamady Ghobashy, Gamal Abdel Nasser Atia, Zubair Ahmad, Farhat S Khan, Paritosh Barai, Md Azizul Haque, Reem Darwesh, Sang Woo Joo","doi":"10.1177/15303667251367426","DOIUrl":"https://doi.org/10.1177/15303667251367426","url":null,"abstract":"<p><p><b><i>Background:</i></b> In view of Corona pandemic, scientists have taken significant efforts to study and recognize the peculiarities of the SARS-CoV-2 outbreak in order to prevent it from spreading. It was discovered that the virus is spreading in many places and nations that have made significant progress in addressing environmental pollution or are not subject to dusty storms. Infections are growing again in the same country, with varied densities of sick persons depending on the weather and windy season. <b><i>Methods:</i></b> Sun and dust are safe techniques for the deactivation and disinfection of SARS-CoV-2. Here, we used metal oxide discs (Fe₂O₃ 9%, SiO₂ 60%, CaO 6%, MgO 8%, and Al₂O₃ 17%) to safely disinfect SARS-CoV-2 after being subjected to the sun for 30 min. As a result, all SARS-CoV-2 were destroyed completely by transmission electron microscope (TEM) after sun exposure. Three major countries have been chosen as case studies: Italy, China, and Iran. <b><i>Conclusion:</i></b> It was discovered that places subjected to dust currents have a lower spread of the coronavirus. This is owing to the fact that the dust contains about 90% metal oxide, which functions as a natural photocatalyst for the synthesis of oxygen and hydrogen. These oxidizing spices can kill SARS-CoV-2.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of <i>Bandavirus dabieense</i> in Bats and Ticks Collected from Bat Habitats.","authors":"Hye-Ryung Byun, Su-Jin Chae, Seong-Ryeong Ji, Jaehyoung Chong, Chul-Un Chung, Bumseok Kim, Hyesung Jeong, Joon-Seok Chae","doi":"10.1177/15303667251376243","DOIUrl":"https://doi.org/10.1177/15303667251376243","url":null,"abstract":"<p><p><b><i>Background:</i></b> The zoonotic infectious disease, severe fever with thrombocytopenia syndrome (SFTS), caused by the SFTS virus (SFTSV), <i>Bandavirus dabieense</i>, was first identified in China in 2009 and reported in the Republic of Korea in 2013. The primary vector is the tick <i>Haemaphysalis</i> (<i>H</i>.) <i>longicornis</i>, which is endemic to the Asia-Pacific region and has a wide range of hosts. While SFTSV has been studied in various wildlife species, no investigation has focused explicitly on bats, which are ecologically significant in the transmission of zoonotic viruses. <b><i>Materials and Methods:</i></b> To investigate the relationship between bats and SFTSV, 1,200 ticks were collected from 12 sites in 6 provinces within 1 km of bat habitats using flagging, and 147 bat sera were collected via cardiac puncture after ether anesthesia between November 2021 and September 2022. Total RNA was extracted from the ticks and bat sera, and nested reverse transcription polymerase chain reaction was performed to amplify the S segment of SFTSV. Bat sera were analyzed for IgG antibodies against SFTSV by enzyme-linked immunosorbent assay (ELISA). <b><i>Results:</i></b> Within 1 km of bat habitats, <i>881 H. longicornis</i>, <i>209 H. flava</i>, 96 <i>Haemaphysalis</i> spp., and 14 <i>Ixodes</i> (<i>I.</i>) <i>nipponensis</i> were identified. SFTSV was detected in 12.3% (147/1,200) of the ticks. Although no SFTSV RNA was detected in bat sera by nested PCR, 3.4% (5/147) were seropositive by ELISA. <b><i>Conclusion:</i></b> While molecular evidence of SFTSV infection was not observed in bats, a few serological positives suggest possible past exposure. The detection of SFTSV in ticks collected from bat habitats suggests potential ecological interactions involving bats, ticks, and other wildlife species. These findings highlight the importance of considering both wildlife reservoirs and the indirect role of bats in the geographical spread of SFTSV.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Molecular Characterization of Two Novel Picorna-Like Viruses in <i>Armigeres subalbatus</i> Mosquitoes in Yunnan, China.","authors":"Qinxuan Miao, Linyu Qu, Jinyong Jiang, Qi Liu, Wenhua Zhao, Litao Tan, Jing Li, Wenjin Cao, Binghui Wang, Xueshan Xia","doi":"10.1177/15303667251372308","DOIUrl":"https://doi.org/10.1177/15303667251372308","url":null,"abstract":"<p><p><b><i>Background</i></b>: Mosquitoes harbor diverse insect-specific viruses (ISVs) frequently overlooked in arbovirus surveillance. Comprehensive characterization of ISVs is crucial for understanding their impact on host ecology and potential roles in arbovirus transmission. <b><i>Methods</i></b>: Using metagenomic sequencing on Armigeres subalbatus from Yunnan, China, we identified two novel picorna-like viruses, assembled their genomes, and conducted phylogenetic analysis. Detection was confirmed via RT-PCR and Sanger sequencing. <b><i>Results</i></b>: Both viruses are closely related to Hubei picorna-like virus 59, previously reported in spiders but unconfirmed. This work provides the first genomic evidence of this lineage in mosquitoes. <b><i>Conclusion</i></b>: This study characterizes two novel ISVs, expanding known picorna-like virus diversity and offering insights into cross-species virus evolution and arbovirus ecology.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145034227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Coxiella burnetii</i> and <i>Coxiella</i> Endosymbiont in Ticks from Western China.","authors":"Yitong Jiang, Xiao Wang, Li Zhong, Junhua Tian, Runda Jie, Yanran Ma, Chunyan Gao, Bing Zhang","doi":"10.1177/15303667251372150","DOIUrl":"https://doi.org/10.1177/15303667251372150","url":null,"abstract":"<p><p><b><i>Background:</i></b> <i>Coxiella</i> spp. are widespread in ticks. <i>Coxiella burnetii</i>, the agents of Q fever, is vectored by ticks and is well known for its veterinary and medical importance. However, reports on <i>C. burnetii</i> in ticks from China have been quite few compared with other tick-borne pathogens. In addition to <i>C. burnetii</i>, more and more <i>Coxiella</i> endosymbionts of ticks have been described in China. <b><i>Materials, Methods, and Results:</i></b> In this study, ticks were collected from domestic animals in two provinces (Chongqing and Xinjiang) in western China, and the <i>Coxiella</i> spp. in them were molecularly studied. In the 168 ticks (all <i>Hyalomma asiaticum</i>) from Xinjiang, <i>C. burnetii</i> was detected with an overall positive rate of 76.19%. In contrast, in 96 ticks (all <i>Rhipicephalus microplus</i>) from Chongqing, only <i>Coxiella</i> endosymbiont was identified with an extremely high prevalence (97.92%). <b><i>Conclusions:</i></b> We propose that there may have been a tick-animal-tick circulation of <i>C. burnetii</i> in Xinjiang. The high positive rate of <i>C. burnetii</i> suggests a high risk to public health.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Identification of Arboviruses from Mosquitoes in Yuxi Region of Yunnan, China.","authors":"Jue Ling, Yantao Zhu, Yuwen He, Shunxiang Li, Chuanzhi Xu, Jinglin Wang","doi":"10.1177/15303667251372145","DOIUrl":"https://doi.org/10.1177/15303667251372145","url":null,"abstract":"<p><p><b><i>Background:</i></b> Arboviruses, transmitted to humans and animals by blood-feeding arthropods, pose significant public health risks. This study investigates the types, distribution, and epidemiology of arboviruses in the central Yuxi region of Yunnan Province, providing crucial data for the prevention and control of mosquito-borne diseases. <b><i>Methods:</i></b> In 2015, mosquitoes were collected from Tonghai County, Huaning County, Jiangchuan District, and Chengjiang County in Yuxi using light traps. The mosquitoes were homogenized, and virus isolation was performed by inoculating the homogenates onto BHK-21 (baby hamster kidney) and C6/36 (<i>Aedes albopictus</i>) cell lines. Reverse Transcription Polymerase Chain Reaction (RT-PCR) amplification was performed with universal and virus-specific primers, followed by sequencing of positive products using bioinformatics tools (DNAstar, MEGA X). <b><i>Results:</i></b> A total of 13,050 mosquitoes from four counties were collected, with 261 pools processed for virus detection. The mosquitoes belonged to three genera: <i>Culex Anopheles</i> and <i>Aedes</i>, with <i>Culex tritaeniorhynchus</i> as the predominant species. Cytopathic effects were observed in 95 pools. Nine viruses were identified: Japanese Encephalitis Virus (JEV), Bannavirus, Getah Virus, Tibet Orbivirus (TIBOV), Nam Dinh Virus, Akabane Virus (AKAV), Ngewotan Virus (NWTV), Quang Binh Virus (QBV), and <i>Culex pipiens pallens</i> densovirus. The distribution of viruses varied: six viruses with 35 isolates in Tonghai County; six viruses with 88 isolates in Huaning County; two viruses with five isolates in Chengjiang County; and no viruses were found in Jiangchuan District. TIBOV and NWTV were present in all three counties, while JEV, AKAV, and QBV were found only in Tonghai County. <b><i>Conclusion:</i></b> Nine arboviruses were isolated across four counties in Yuxi, with varying distributions. This highlights the epidemiological diversity of mosquito-borne viruses in the region, emphasizing the need for targeted control measures.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144971230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}