Vector borne and zoonotic diseases最新文献

{"title":"Optimized Sindbis Virus RNA Detection Using Novel RT-qPCR: Isolation and Characterization of Sindbis Virus from a Patient Sample.","authors":"Eveliina Ekström, Katariina Kaansalo, Phuoc T Truong Nguyen, Maija T Suvanto, Olli Vapalahti, Hannimari Kallio-Kokko, Essi Korhonen, Hanna Vauhkonen, Teemu Smura, Eili Huhtamo, Anne J Jääskeläinen","doi":"10.1089/vbz.2025.0037","DOIUrl":"https://doi.org/10.1089/vbz.2025.0037","url":null,"abstract":"<p><p><b><i>Background:</i></b> Strains of Sindbis virus (SINV) are rarely detected and isolated from human blood samples as the viremia is low and short in Pogosta disease patients. <b><i>Materials and Methods:</i></b> To optimize SINV RNA detection in patient samples, a sensitive and specific SINV-RT-qPCR method was developed and validated. <b><i>Results:</i></b> The new SINV-RT-qPCR method was determined to be more sensitive than a previously used method. The assay was used to screen 882 serum samples from 846 patients suspected of Pogosta disease in Finland in 2023-2024. One sample was detected to be SINV-RNA-positive and was further subjected to virus isolation with whole genome sequencing. The sequence analysis suggested the strain to be closely related to those detected in Finland in 2005 and 2018, Germany in 2012 and 2016, and Sweden in 2009. <b><i>Conclusions:</i></b> The new optimized SINV RNA detection method is a useful tool for the detection of viremic samples for further studies.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Entry of Zika Virus into the Cells and Its Implication for Antiviral Therapy.","authors":"Nahla Ahmed Hassaan, Li Xing","doi":"10.1089/vbz.2024.0084","DOIUrl":"https://doi.org/10.1089/vbz.2024.0084","url":null,"abstract":"<p><p><b><i>Background:</i></b> Zika virus (ZIKV) belongs to the <i>Flavivirus</i> genus of the <i>Flaviviridae</i> family. It is an enveloped RNA virus that enters host cells via receptor-mediated endocytosis. The interactions between viral proteins and particular receptors on the host cell surface is the initial step of the virus life cycle, which represents the key targets for antiviral therapeutic. <b><i>Materials and Methods:</i></b> This review highlights a variety of cell types infected by ZIKV, including human radial glial cells, endothelial cells, neural progenitor cells, astrocytes, microglia, and Sertoli cells. The cellular molecules involved in the entry process of ZIKV are detailed, and the advances in the development of chemical compounds and neutralizing antibodies targeting the ZIKV entry process are described. <b><i>Results:</i></b> The interactions of ZIKV with cellular molecules in various host cells during virus entry are reviewed, as the targets of the development of antiviral therapeutics. <b><i>Conclusion:</i></b> The entry of ZIKV into host cells involves complicated mechanisms, which remain to be further explored to facilitate the development of antiviral reagents.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between CD55 Polymorphisms and Susceptibility to Uncomplicated Malaria Imported from Africa in a Chinese Han Population.","authors":"Chao Xu, Hongjie Dong, Ting Xiao, Song Zhu, Fan Bai, Qi Wang, Wenju Zhu, Hui Sun, Jin Li, Guihua Zhao, Yongbin Wang, Qingkuan Wei, Kun Yin","doi":"10.1089/vbz.2024.0132","DOIUrl":"https://doi.org/10.1089/vbz.2024.0132","url":null,"abstract":"<p><p><b><i>Background:</i></b> The increasing number of imported malaria cases has become a serious public health issue in China. However, the relationship between human genetic variations and malaria susceptibility within the Chinese Han population remains insufficiently understood. This study aims to investigate the contribution of CD55 polymorphisms to the susceptibility of uncomplicated malaria infection imported from Africa in Han Chinese individuals. <b><i>Materials and Methods:</i></b> This case-control study included 348 patients with uncomplicated <i>Plasmodium falciparum</i> (<i>P. falciparum</i>) infections imported from Africa and 276 healthy controls from a Chinese population. Genotyping of CD55 polymorphisms rs2564978, rs6691942, and rs1507765 was conducted using a Sequenom MassARRAY system. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated through nonconditional logistic regression analysis. Haplotype and linkage disequilibrium tests were performed using the Haploview 4.2 software. <b><i>Results:</i></b> Of the 624 samples, 619 were successfully genotyped for rs2564978, and 620 were successfully genotyped for rs6691942 and rs1507765. A statistically significant difference in genotype distribution between the case and control groups was observed for the rs2564978 polymorphism. Specifically, the heterozygous CT genotype (<i>p</i> = 0.035) and the dominant genetic model CC + CT versus TT (<i>p</i> = 0.044, OR = 1.441, 95% CI: 1.010-2.056) of rs2564978 were associated with an increased risk of uncomplicated malaria, respectively. Haplotype analysis revealed that rs2564978 and rs6691942 were in linkage disequilibrium. <b><i>Conclusion:</i></b> The findings suggested that the rs2564978 polymorphism of the CD55 gene may increase the risk of uncomplicated malaria imported from Africa in the Chinese Han population.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Fecal Carriage of <i>Helicobacter</i> Species Among Pet Animals Suffering from Gastroenteritis: A Potential Public Health Concern.","authors":"Aisha A Abd Al-Ghafar, Sherif A Marouf, Khaled A Abdel-Moein, Hala M Zaher, Ahmed Samir","doi":"10.1089/vbz.2025.0005","DOIUrl":"https://doi.org/10.1089/vbz.2025.0005","url":null,"abstract":"<p><p><b><i>Background:</i></b> Although recent research has provided better insights into <i>Helicobacter pylori</i>, much remains unknown regarding non-<i>H. pylori Helicobacter</i> species in animals. Pet animals living close to humans may act as a potential reservoir for a diverse <i>Helicobacter</i> species. Hence, this study aimed to investigate <i>Helicobacter</i> spp.'s prevalence among pet animals suffering from gastroenteritis and their zoonotic relevance. <b><i>Materials and methods:</i></b> Eighty-seven fecal samples were collected from dogs (53) and cats (34) suffering from gastroenteritis. The DNA was extracted for the molecular detection of <i>Helicobacter</i> spp., <i>H. canis</i>, <i>H. felis</i>, and <i>H. pylori</i> through amplification of <i>Helicobacter</i> 16S rRNA as well as multiplex PCR targeting <i>H. canis</i> Hsp60, <i>H. felis</i> urease, and <i>H. pylori</i> urease C genes in all samples. Sequencing of some selected PCR amplicons and phylogenetic analysis were conducted. <b><i>Results:</i></b> The overall prevalence of <i>Helicobacter</i> spp. ws 34.5%, with the following rates: 37.7% and 29.4% among dogs and cats, respectively. <i>H. canis</i> had the highest occurrence rate (20.7%), whereas <i>H. pylori</i> was detected at 11.5%, followed by <i>H. felis</i> at 2.3%. Coinfection with more than one <i>Helicobacter</i> species has been documented. Furthermore, the phylogenetic analysis of three partial <i>H. canis</i> 16S rRNA sequences revealed a high genetic relatedness to strains derived from a diarrheic cat and human bacteremic patients with a history of pet exposure, indicating the public health implications of these sequences. <b><i>Conclusion:</i></b> The fecal carriage of diverse <i>Helicobacter</i> spp. among dogs and cats suffering from gastroenteritis, with a predominance of <i>H. canis</i>, highlights a potential risk of zoonotic transmission of such pathogens between pets and human contacts.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and Specific Detection/Identification of <i>Leishmania major</i>, <i>Leishmania tropica</i>, and <i>Leishmania infantum</i> by a Single Real Time PCR Targeting <i>Hsp70</i>.","authors":"Reza Mohammadi Manesh, Parisa Mousavi, Mahshid Shakibapour, Mehdi Mohebali, Behrooz Ataei, Hossein Mirhendi","doi":"10.1089/vbz.2024.0087","DOIUrl":"https://doi.org/10.1089/vbz.2024.0087","url":null,"abstract":"<p><p><b><i>Backgrounds:</i></b> Leishmaniasis is one of the neglected tropical diseases, distributed across 89 countries in both the Old and New Worlds. Among the 54 identified <i>Leishmania</i> species, 21 are known to be pathogenic to humans. Cutaneous leishmaniasis (CL) is primarily caused by <i>L. major</i> and <i>L. tropica</i>, while visceral leishmaniasis (VL) in Iran is caused by <i>L. infantum</i>. Accurate detection and species identification of <i>Leishmania</i> spp. are crucial for more effective treatment, epidemiology, and control strategies for the disease. Among the molecular targets recently used for detecting <i>Leishmania</i> species, the heat-shock protein 70 (<i>Hsp70</i>) gene has proven to be highly suitable. <b><i>Methods:</i></b> This study aimed to establish and evaluate a SYBR Green real-time PCR targeting the <i>Hsp70</i> gene to identify and differentiate three <i>Leishmania</i> species: <i>L. major</i>, <i>L. tropica</i>, and <i>L. infantum</i> in clinical specimens. A total of 219 microscopic smears, consisting of both positive and negative leishmaniasis cases diagnosed by microscopy, were subjected to DNA extraction and the <i>Hsp70</i> real-time PCR assay designed in this study. <b><i>Results:</i></b> Based on the analysis of the melting temperature (T_m) of the amplified <i>Hsp70</i> target, 115 microscopy-positive smears were identified, comprising 70.4% <i>L. major</i>, 23.5% <i>L. tropica</i>, and 6.1% <i>L. infantum</i>. All results were confirmed using a commercial diagnostic kit. Sanger sequencing of selected positive amplicons unequivocally confirmed the accuracy of this method in identifying and distinguishing the three <i>Leishmania</i> species. <b><i>Conclusions:</i></b> The <i>Hsp70</i> real-time PCR can be considered an effective method for detecting and identifying <i>Leishmania</i> species from microscopic slides prepared from CL and VL cases in different regions of Iran.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of a Blood Meal on <i>Plasmodium</i> Oocyst Growth Using the Enema Injection Method.","authors":"Asako Haraguchi, Moeka Gonda, Kazuhiko Nakayama, Kanta Fujiwara, Jun Hakozaki, Sakure Nakamura, Kodai Kusakisako, Hiromi Ikadai","doi":"10.1089/vbz.2024.0099","DOIUrl":"10.1089/vbz.2024.0099","url":null,"abstract":"<p><p><b><i>Objective:</i></b> <i>Anopheles</i> mosquitoes transmit <i>Plasmodium</i> parasites through blood feeding. The oocyst stage in mosquitoes is crucial for <i>Plasmodium</i> transmission. Oocysts can form without a blood meal or the midgut passage and utilize nutrients from additional blood feeding. However, it remains unclear the impact of a blood meal during infection on oocysts. The present study evaluated how a blood meal during infection impacts oocyst growth to better understand oocyst development. <b><i>Methods:</i></b> We used a novel method for <i>Plasmodium</i> infection known as enema injection, which involves injecting <i>Plasmodium berghei</i> (ANKA strain) into the midgut lumen of <i>Anopheles stephensi</i> mosquitoes (STE2 strain) via the anus, traversing the midgut wall without a blood meal. We compared the size of oocysts in mosquitoes infected by enema injection alone, hemocoel injection alone, and each infection method combined with uninfected blood feeding, as well as those only with infected blood feeding. <b><i>Results:</i></b> By enema injection with <i>Plasmodium</i> ookinetes, oocysts formed solely in the mosquito's midgut. Oocysts from enema-injected mosquitoes were similar in size to those from hemocoel-injected mosquitoes. Oocysts from mosquitoes infected by enema injection combined with uninfected blood feeding were larger than oocysts from mosquitoes infected by enema injection alone. However, the size of oocysts from mosquitoes infected by hemocoel injection was not affected by the presence or absence of blood feeding. <b><i>Conclusion:</i></b> Enema injection with <i>Plasmodium</i> ookinetes is applicable to <i>Anopheles</i> mosquitoes. Using the enema injection method, we suggest that a blood meal during infection might facilitate oocyst growth within the midgut.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"408-415"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144030193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Survey and Phylogenetic Characterization of Tick-Borne Pathogens in Hubei Province, China.","authors":"Yake Lei, Ying Dai, Meng Guo, Lei Yi, Kun Cai, Bing Hu","doi":"10.1089/vbz.2024.0126","DOIUrl":"10.1089/vbz.2024.0126","url":null,"abstract":"<p><p><b><i>Background:</i></b> Tick-borne diseases pose a serious disease burden globally, with pathogens including viruses, bacteria, rickettsia, and spirochetes. This study aims to investigate ticks and pathogens in Huanggang and Suizhou, two regions with the highest tick-borne disease prevalence in Hubei, China. <b><i>Methods:</i></b> A total of 2,958 ticks were collected, and their species diversity was characterized. RNA and DNA were used for polymerase chain reaction detection and sequencing of <i>Dabie bandavirus</i> (DBV), spotted fever group rickettsioses (SFGR), <i>Coxiella burnetii</i>, <i>Borrelia</i>, <i>Anaplasma phagocytophilum</i>, <i>Babesia</i>, and <i>Bartonella</i> pathogens in ticks. Molecular evolutionary traits and phylogenetic linkages were further analyzed. <b><i>Results:</i></b> One family, four genera, and four species of ticks were identified, with <i>Haemaphysalis longicornis</i> being the predominant species. The overall DBV-positive rate was 2.63% in ticks from Huanggang City and 0.48% in those from Suizhou City. Phylogenetic analysis indicated that the DBV strains from Hubei Province belonged to genotypes A-F, with high evolutionary homology with those sampled from local human patients. The tick-borne SFGR-positive rate was significantly higher in samples from Huanggang City (48.23%) than in Suizhou City (8.29%). High levels of <i>A. phagocytophilum</i> sequences were detected in both regions, with 99% homologous. <i>Borrelia burgdorferi</i> was detected for the first time in Suizhou City, with a very low carriage rate (0.24%), and was not detected in Huanggang City. No <i>Bartonella</i>, <i>Babesia</i>, or <i>C. burnetii</i> pathogens were detected from either location. <b><i>Conclusions:</i></b> This study represents a substantial effort to investigate the diversity of tick-borne pathogens in Huanggang and Suizhou City. Our findings provide references and data to help develop effective tick-borne disease prevention, surveillance, and early warning systems.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"377-389"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characteristics of Elderly Patients with Hemorrhagic Fever with Renal Syndrome: A Retrospective Study Conducted in Shaanxi Province, China.","authors":"XiaoQin Cao, YaFei Jin, Hong Du, Wei Wang, YanYan Zhao, XuYang Zheng, XiaoYan Wang, PeiYu Bian, LiMei Wang, Hong Jiang","doi":"10.1089/vbz.2024.0063","DOIUrl":"10.1089/vbz.2024.0063","url":null,"abstract":"<p><p><b><i>Objective:</i></b> To study the clinical and laboratory characteristics of hemorrhagic fever with renal syndrome (HFRS) in Chinese elderly patients. <b><i>Methods:</i></b> The clinical data of hospitalized patients diagnosed with HFRS from 2011 to 2022 were extracted from the inpatient record system of the Second Affiliated Hospital of Air Force Medical University. A comparative analysis was conducted between young and elderly individuals regarding the male-to-female ratio, disease classification, clinical laboratory indices, and prognosis. <b><i>Results:</i></b> Among the patients diagnosed with HFRS, the elderly population accounted for 14.86%, comprising 199 males and 86 females. The proportion of severe-type cases among elderly patients was 25.96%, while the proportion of critical cases was 32.63%. Critically, the proportion of critical-type cases in this age group was significantly higher compared to individuals aged 20-60 years. Comparing the clinical indices of elderly patients with HFRS with different clinical outcomes, our findings revealed deceased cases exhibited significantly higher body temperature, hemoglobin (HGB) levels, and white blood cell (WBC) counts compared to surviving patients. Conversely, albumin (ALB) levels, fibrinogen (FIB) levels, and platelet counts were significantly lower in deceased patients than in surviving patients. <b><i>Conclusion:</i></b> The incidence of HFRS in the elderly presents a higher proportion of severe-type and critical-type cases and a greater mortality rate compared to younger individuals. Elevated body temperature, HGB levels, and WBC counts, and lower ALB and FIB levels suggest a poorer prognosis.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"416-423"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hospital-Based Surveillance of Rickettsial Diseases in Kashmir, North India.","authors":"Bashir Ahmad Fomda, Yawar Bashir Mir, Mudasir Qadri Syed, Nazima Abdullah, Mubashir Nazir, Mushtaq Ahmad Bhat, Sonaullah Shah","doi":"10.1089/vbz.2024.0122","DOIUrl":"10.1089/vbz.2024.0122","url":null,"abstract":"<p><p><b><i>Background:</i></b> Rickettsial diseases were reported from Kashmir, India, in the early 20th century. Since then, limited research was carried that has resulted in a significant gap in understanding these diseases. This study was conducted to find out the prevalence and diversity of rickettsial diseases in this region. <b><i>Methods:</i></b> Blood sample from 476 patients were collected. Enzyme-linked immunosorbent assay was done for the detection of IgM and IgG, and immunofluorescence assay was done for endpoint titer determination. Nested polymerase chain reaction was performed for detection of spotted fever group (gltA, sca0, sca5 genes), typhus group (17 kDa gene) scrub typhus group (47 and 56 kDa gene), followed by sequencing and phylogenetic analysis. <b><i>Results:</i></b> The seropositivity of scrub typhus, spotted fever group, typhus group was 74 (15.54%), 71 (14.91%), and 60 (12.60%), respectively. Scrub typhus was significantly associated with the urban population (odds ratio = 1.812, 95% confidence interval 1.099-3.0, <i>p</i> = 0.02). Bimodal seasonal variation was observed in all the three groups. In spotted fever group, out of 48 samples 81.25%, 45.83%, and 38.09% were positive for gltA, sca0, and sca5 genes, respectively, and in typhus group 20.83% were positive for 17 kDa. In the scrub typhus group, out of 62 samples, the 56 kDa gene was positive in 56.45%, while the 47 kDa gene was positive in 35.48%. Phylogenetic analysis revealed <i>Rickettsia conorii</i>, <i>Rickettsia parkeri</i>, <i>Rickettsia typhi</i>, and <i>Orientia tsutsugamushi</i>. <b><i>Conclusions:</i></b> This study confirms the presence of human rickettsial infections in Kashmir, highlighting the need for including rickettsial diseases in diagnostic algorithm for acute febrile illness.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"390-402"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural <i>Trypanosoma cruzi</i> Infection in Triatomines (Hemiptera: Reduviidae: Triatominae) in an Ecotourism Area of the Atlantic Forest in São Paulo State.","authors":"Jaciara de Oliveira Jorge Costa, Herbert Sousa Soares, Isabela Pereira Pesenato, Thiago Fakelmann, Giovanna Stefani Nosberto Castelli, Arlei Marcili","doi":"10.1089/vbz.2024.0078","DOIUrl":"10.1089/vbz.2024.0078","url":null,"abstract":"<p><p><b><i>Background:</i></b> Triatomines (Hemiptera: Reduviidae: Triatominae), which are the vectors of Chagas disease in Brazil, are associated with forested areas. This study analyzed triatomines and their natural infection by Trypanosoma cruzi in an Atlantic Forest remnant in the state of São Paulo, Brazil. <b><i>Materials and Methods:</i></b> Thirty-five triatomines were captured and identified as Triatoma tibiamaculata and Panstrongylus megistus. The study included direct trypanosome research and isolation in Balb/c mice. <b><i>Results:</i></b> Fifteen isolates were obtained from seven triatomines representing both species. Protein sequences from the V7-V8 SSUrDNA region were obtained from the isolates and positive triatomines and were analyzed using maximum parsimony and Bayesian methods for phylogenetic positioning. All sequences obtained were identified as T. cruzi; the isolates were classified as TcII, whereas a direct sample was typed as TcIV. <b><i>Conclusion:</i></b> The results demonstrated the occurrence of active transmission cycles and the risk of vector transmission to the population in these regions.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"424-426"},"PeriodicalIF":1.8,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}