Vector borne and zoonotic diseases最新文献

{"title":"Molecular Detection and Phylogenetic Analysis of <i>Anaplasma</i> Species in Ticks from Three Provinces of China.","authors":"Yuqing Cheng, Qinghong Yuan, Meng Yang, Junrong Liang, Zhongqiu Teng, Qingzhu Huang, Miao Lu, Tian Qin","doi":"10.1089/vbz.2024.0134","DOIUrl":"https://doi.org/10.1089/vbz.2024.0134","url":null,"abstract":"<p><p><b><i>Background:</i></b> Anaplasmosis, caused by Anaplasma species, poses significant threats to public health and livestock productivity. Understanding the distribution and genetic diversity of these pathogens in tick vectors across China is critical for risk assessment and disease control. <b><i>Materials and Methods:</i></b> From April to June 2023, 875 ticks were collected across three Chinese provinces: Jiangxi, Yunnan, and Shaanxi. The collected ticks comprised Rhipicephalus microplus (from Jiangxi and Yunnan) and Haemaphysalis longicornis (from Shaanxi). Pathogen detection was performed using PCR, followed by phylogenetic analysis of the obtained sequences to determine genetic relationships. <b><i>Results:</i></b> Anaplasma capra was detected predominantly in R. microplus from Yudu County, Jiangxi (4.95%), and in H. longicornis from Meixian County (3.16%), Long County (5.99%), and Zhenba County (0.83%) in Shaanxi, exhibiting regional genetic variations. The detection rate of Anaplasma marginale was 6.59% in Yudu County, Jiangxi; significantly higher rates were found in Yunnan province: 41.75% in Nanjian County, 40.38% in Weishan County, and 52.04% in Yongsheng County. Phylogenetic analysis revealed that A. marginale isolates from Lijiang (Yongsheng), Yunnan, were highly homologous (99.63%-100%) to those from Yudu County, Jiangxi, while isolates from Dali (Nanjian and Weishan), Yunnan, formed a distinct clade. <b><i>Conclusion:</i></b> The findings demonstrate the widespread distribution of A. capra and A. marginale in ticks across the surveyed regions of China, with notable variations in prevalence and genetic characteristics. These pathogens represent potential threats to local residents and livestock. Future research should expand the geographic sampling range to fully understand their distribution patterns and explore effective prevention and control strategies to safeguard public health and safety.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xenomonitoring and Molecular Characterization of <i>Brugia malayi</i> from <i>Mansonia annulifera</i> in Alappuzha, Kerala, India.","authors":"Kiruthiga Tamilselvan, Aiswarya R Suresh, Sivalaxmi Balakrishnan, Harish Kumar Shah, Bhairavi Balasubramanian, Jessu Mathew, Hisham Moosan","doi":"10.1089/vbz.2025.0015","DOIUrl":"10.1089/vbz.2025.0015","url":null,"abstract":"<p><p><b><i>Background:</i></b> Lymphatic filariasis is a major public health problem known for its disfiguring and debilitating effects. Alappuzha district in Kerala has been a hotspot for Brugian and Bancroftian filariasis. The efforts to combat Brugian filariasis in this region about two decades earlier led to notable decline in vector density and interruption of disease transmission. <b><i>Materials and Method:</i></b> A cross-sectional survey was carried out in Alappuzha district and 12 wards were selected using multistage random sampling method. An entomological survey was conducted in the selected wards. <b><i>Result:</i></b> Fourteen out of 69 Ma. annulifera were positive for filarial parasites. The average infection and infectivity rate was 20% and 0%. Molecular characterization of positive samples confirmed the parasites as Brugia malayi. Phylogenetic analysis of the isolates revealed that they belong to the same clade. <b><i>Conclusion:</i></b> This study highlights the importance of continued vector surveillance and timely intervention by appropriate vector control measures to prevent transmission of the disease.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"476-479"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Burden of Agriculturally Significant Vector-Borne and Parasitic Diseases in Kansas.","authors":"Kristin Michel, Nicole M Ioerger, Ashlie M Ake, Susan M Hettenbach, Cassandra Olds, Dustin L Pendell, James Stack, Stephen Higgs, Dana L Vanlandingham","doi":"10.1089/vbz.2025.0023","DOIUrl":"10.1089/vbz.2025.0023","url":null,"abstract":"<p><p><b><i>Background:</i></b> The state of Kansas (KS) has been called the \"agricultural heartland\" of the United States. Vector-borne and parasitic diseases (VBPD) have a major impact on the production of livestock, such as cattle, swine, goats and sheep, as well as crops, such as wheat, corn, and sorghum. The purpose of this review is to educate agricultural professionals in the state of KS about VBPD of current or potential concern and to inform the public about the challenges faced by the agricultural community. <b><i>Methods:</i></b> This review describes and discusses the endemic VBPD that currently impact agricultural production in KS and foreign VBPD of concern. In addition, we outline the major arthropod vectors of VBPD in KS, including ticks, mites, and various insects. In the context of this review, parasites are strictly limited to arthropod ectoparasites that negatively impact livestock production. Modern agricultural data for the state of KS were mostly sourced from the USDA National Agricultural Statistics Service, and current KS VBPD data were mostly sourced from the KS State Veterinary Diagnostic Laboratory. <b><i>Conclusion:</i></b> These VBPD have a large economic impact on the state and country, and we have concluded there is a need for updated estimates regarding the economic burden of VBPD in KS and throughout the United States to make better animal and crop health investment decisions.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"429-443"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Characteristics of Five Cases of <i>Chlamydia psittaci</i> Pneumonia Diagnosed Using Metagenomics Next-Generation Sequencing.","authors":"Wei Wang, Chun-Liang Yan, Qi-Shan Xue","doi":"10.1089/vbz.2025.0009","DOIUrl":"10.1089/vbz.2025.0009","url":null,"abstract":"<p><p><b><i>Objective:</i></b> This study aimed to analyze the clinical characteristics of five cases of <i>Chlamydia psittaci</i> pneumonia diagnosed using metagenomics next-generation sequencing (mNGS) and to provide help for its diagnosis and treatment. <b><i>Methods:</i></b> Five patients who were admitted to the department of Respiratory and Critical Care Medicine of Beijing Aerospace General Hospital between June 2020 and December 2021 and diagnosed with <i>C. psittaci</i> pneumonia using mNGS were selected, including one case with severe pneumonia. We analyzed the clinical characteristics, epidemiology, laboratory results, treatment, and prognosis of the five participating patients. <b><i>Results:</i></b> The main clinical manifestations were high fever, dry cough, chest tightness, shortness of breath, and myalgia. All five patients had a history of contact with poultry. The white blood cell count was normal or slightly increased, the lymphocyte count was significantly decreased, and the percentage of neutrophil granulocyte, C-reactive protein (CRP), erythrocyte sedimentation rate, and procalcitonin were significantly increased. Chest computed tomography showed inflammatory infiltration and consolidation of one or more lung lobes, possibly accompanied by pleural effusion and liver function impairment. Bronchoscopy mainly showed congestion and edema of airway mucosa with less sputum in the airway. In all patients, we detected the nucleic acid sequences of <i>C. psittaci</i> in alveolar lavage fluid or sputum using mNGS and confirmed the diagnosis of <i>C. psittaci</i> pneumonia based on clinical manifestations and exposure history. After adjusting antibiotics to a moxifloxacin-based treatment regimen, the disease improved. The application of the mNGS assay enables us to make faster diagnoses of diseases so that timely medication can be administered, thus shortening the duration of a patient's illness. <b><i>Conclusion:</i></b> <i>C. psittaci</i> pneumonia has an acute onset, and fever and cough are common symptoms. A history of contact between the patient and birds is an important diagnostic clue; however, clinical symptoms and laboratory and imaging examinations lack specificity. The detection using mNGS of bronchoalveolar lavage fluid can quickly confirm the diagnosis and reduce missed diagnoses and misdiagnoses. Moxifloxacin-based therapy is effective, and patients require combination therapy if they have other bacterial infections.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"463-469"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Detection and Genome Analysis of the Kindia Tick Virus in Cattle and <i>Rhipicephalus</i> Ticks in the Republic of Guinea.","authors":"Mikhail Yu Kartashov, Ekaterina I Krivosheina, Ekaterina V Naidenova, Kirill S Zakharov, Alexander N Shvalov, Sanaba Boumbaly, Vladimir A Ternovoi, Valery B Loktev","doi":"10.1089/vbz.2024.0056","DOIUrl":"10.1089/vbz.2024.0056","url":null,"abstract":"<p><p><b><i>Background:</i></b> Over the past decade, numerous new tick-associated flavi-like viruses with segmented genomes have been discovered almost worldwide. Kindia tick virus (KITV) was first detected in <i>Rhipicephalus geigyi</i> ticks in West Africa in 2017. The current study aimed to detect viral RNA in tick and cattle samples collected in Guinea and to perform complete sequencing of KITV isolates and their analysis. <b><i>Methods:</i></b> Adult ticks and blood samples were collected from cattle in Coyah, Dubréka, Forécariah, and Kindia prefectures of the Republic of Guinea in 2022. These samples were tested for KITV infection by RT-PCR with primers targeting the NS5 gene. Positive probes were sequenced using Illumina technology, and their analysis was performed for obtaining complete sequences of all KITV segments. <b><i>Results:</i></b> The RNA of the KITV was detected by RT-PCR in <i>Rh. geigyi</i>, <i>Rh. annulatus</i> ticks, and blood samples of cattle. The prevalence rates for cattle were 6.6%, for <i>Rh. annulatus</i> 6.9%, and for <i>Rh. geigyi</i> ticks 10.7%. The analysis of 15 complete sequences of KITV genomes showed 99.61-99.67% identity for amino acid sequences for segments 1 and 4 and 97.88-98.83% for segments 2 and 3 with previously detected KITV isolate in Guinea in 2017. Phylogenetic analysis demonstrated that obtained KITV sequences can be classified as typical for clade A of the Jingmen tick virus (JMTV) group together with Mogiana tick virus. <b><i>Conclusion:</i></b> The KITV isolates from cattle and feeding ticks show practically full identity sequences for all four viral segments, and these sequences can be classified as clade A of the segmented flavi-like virus JMTV group.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"470-475"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Detection of <i>Francisella</i> spp. in Blood, Milk, and Ticks Infesting Small Ruminants in Urmia, Iran.","authors":"Zahraalsadat Tavakolian, Amir Tukmechi, Abdulghaffar Ownagh, Ahmad Enferadi","doi":"10.1089/vbz.2025.0007","DOIUrl":"10.1089/vbz.2025.0007","url":null,"abstract":"<p><p><b><i>Background:</i></b> Zoonotic diseases pose a significant global health challenge, accounting for over half of all newly emerging infectious illnesses. One such pathogenic agent is the bacterium Francisella tularensis, which causes tularemia, a zoonotic disease spread in the Northern Hemisphere through various means, including tick bites. Unfortunately, there is limited literature on tick-borne bacteria affecting human and animal health. This research confirmed the presence of Francisella spp. in ticks in the Urmia region, highlighting the potential for ticks to act as vectors for transmitting the bacteria to livestock and humans. <b><i>Materials and Methods:</i></b> A total of 397 ticks, 315 blood samples, and 241 milk samples were gathered from sheep and goats to screen for the existence of Francisella spp. The nested PCR technique revealed the existence of Francisella spp. 16S rRNA gene in the samples. <b><i>Results:</i></b> Blood and milk samples from small ruminants did not show the presence of Francisella spp., but the bacteria were detected in 6 tick pools (<i>n</i> = 79; 7.59%; 95% CI: 3.52%-15.59%). The positive ticks identified were Hyalomma asiaticum 2 (<i>n</i> = 24; 8.33%; 95% CI: 2.31%-25.84%), Hyalomma eagyptum 3 (<i>n</i> = 9; 33.33%; 95% CI: 12.06%-64.58%), and Rhipicephalus sanguineus 1 (<i>n</i> = 15; 6.67%; 95% CI: 1.19%-29.82%) and the presence of Francisella tularensis subspecies holarctica was found to be positive in 3 out of 79 tick pools (3.80%; 95% CI: 1.3%-10.58%). The positive ticks identified in this experiment were female Hyalomma aegyptium (1 out of 3; 33.33%; 95% CI: 6.15%-79.23%), male Hyalomma asiaticum (1 out of 14; 7.14%; 95% CI: 1.27%-31.47%) and female Rhipicephalus sanguineus (1 out of 7; 14.29%; 95% CI: 2.57%-51.32%). <b><i>Conclusion:</i></b> The study explored the presence of Francisella species, especially the most dangerous, F. tularensis, in small ruminants and the ticks that attach to them using molecular techniques in the Urmia region of northwestern Iran. The research seeks to improve knowledge of Francisella spp. epidemiology. Understanding the risk of human-livestock transmission of this pathogen is crucial for public health, particularly for individuals working in animal-related fields.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"454-462"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144037091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of Lyme Disease in Asian Human Populations: A Systematic Review and Meta-Analysis.","authors":"Jieqin Song, Yan Dong, Yu Zhang, Guozhong Zhou, Xinya Wu, Li Gao, Hanxin Wu, Li Peng, Jiaru Yang, Zhenhua Ji, Bingxue Li, Yuxin Fan, Jingjing Chen, Meixiao Liu, Jing Kong, Weijie Ma, Lei Zhong, Weijiang Ma, Aihua Liu, Fukai Bao","doi":"10.1089/vbz.2024.0092","DOIUrl":"10.1089/vbz.2024.0092","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;&lt;i&gt;Background:&lt;/i&gt;&lt;/b&gt; Lyme disease (LD, also known as Lyme borreliosis) is the most frequent tick-transmitted disease caused by the spirochete &lt;i&gt;Borrelia&lt;/i&gt; in Europe and the United States. LD is distributed in the Northern Hemisphere, but the seroprevalence of LD in Asian human populations is unclear. &lt;b&gt;&lt;i&gt;Objectives:&lt;/i&gt;&lt;/b&gt; To investigate the seroprevalence of LD in Asian human populations. &lt;b&gt;&lt;i&gt;Data Sources:&lt;/i&gt;&lt;/b&gt; PubMed, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL), and other sources were searched for relevant studies with MeSH terms from their inception up to 20 June 2022. &lt;b&gt;&lt;i&gt;Study Eligibility Criteria:&lt;/i&gt;&lt;/b&gt; Cross-sectional studies with no language restrictions. &lt;b&gt;&lt;i&gt;Participants:&lt;/i&gt;&lt;/b&gt; Healthy people, at-risk people, and patients with suspected LD. Moreover, the seroprevalence of LD was diagnosed by laboratory diagnosis (nzyme-linked immunosorbent assays (ELISA)/Immunofluorescence assays (IFA) or/and two-tier testing) in human populations. &lt;b&gt;&lt;i&gt;Assessment of Risk of Bias:&lt;/i&gt;&lt;/b&gt; Risk of bias was rated using the Joanna Briggs Institute (JBI) standardized critical appraisal instrument for prevalence studies (Critical Appraisal Checklist for Analytical Cross-Sectional Studies). &lt;b&gt;&lt;i&gt;Methods of Data Synthesis:&lt;/i&gt;&lt;/b&gt; Seroprevalence and proportion of LD in human populations in Asia were obtained from the included studies. Two authors independently screened and selected studies according to our predefined criteria (PROSPERO CRD42022362029) and assessed their risk of bias. A third author was available for arbitrating discrepancies. A random-effects model meta-analysis was conducted to determine the proportions of LD and related information, and further subgroup analyses of some studies were conducted, such as methods for diagnosing LD, gender, and human populations with and without tick bites. &lt;b&gt;&lt;i&gt;Results:&lt;/i&gt;&lt;/b&gt; There are 18 studies included after full-text screening and 11,498 people in the meta-analysis. These studies encompassed countries such as China, Japan, Korea, Türkiye, Singapore, and Indonesia. Regarding the risk of bias and the JBI checklist, 2 studies scored 7 points and 16 studies scored 8 points. All studies were rated as high quality (≥5 points). In the meta-analysis, the seroprevalences of LD were 12.1% (95% confidence interval [CI] 0.081-0.168) by ELISA/IFA and 5.7% (95% CI 0.034-0.085) for two-tier seropositivity testing in Asia. In subgroup analyses, the proportion of those diagnosed with LD by ELISA/IFA (14.7%, 95% CI 0.094-0.208) was significantly higher than the proportion diagnosed by two-tier testing (5.9%, 95% CI 0.032-0.095) (&lt;i&gt;p&lt;/i&gt; &lt; 0.01). The proportion of LD (two-tier testing) was slightly higher in women (7.4%, 95% CI 0.036-0.123) than in men (6.2%, 95% CI 0.026-0.111), but the difference was not significant (&lt;i&gt;p&lt;/i&gt; = 0.70). In the study population, 47% (95% CI 0.159-0.795) were bitten by ticks (people with confirmed tick bites). The difference in the prop","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":"444-453"},"PeriodicalIF":1.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Babesia</i> Infections in Reservoir Hosts and the Possibility of Human Babesiosis in Ghana.","authors":"Linda A O Amoah, Evans P K Ameade, Benjamin Yeboah Ofori","doi":"10.1089/vbz.2024.0120","DOIUrl":"https://doi.org/10.1089/vbz.2024.0120","url":null,"abstract":"<p><p><b><i>Background:</i></b> In Ghana, no research has recorded any positive cases of human babesiosis, despite the presence of <i>Babesia</i> reservoir hosts in many Ghanaian communities. Epidemiological studies have reported active infections in these hosts. Thus, this study explored possible cases of human babesiosis in selected major hospitals in the Greater Accra region and assessed the prevalence of <i>Babesia</i> infections in common reservoirs (dogs, cats, and rodents) from households and veterinary institutions. <b><i>Methods:</i></b> This cross-sectional study involved retrieving medical records from three major hospitals in urban (University of Ghana Hospital and Pentecost Hospital) and peri-urban (Shai-Osudoku District Hospital) areas that satisfied the eligibility criteria. Analysis of human babesiosis cases was conducted using appropriate search queries in Microsoft Excel. Genomic DNA was also isolated from 404 blood samples from <i>Babesia</i> reservoir hosts, and DNA analysis was performed using PIRO A1 and PIRO B primers targeting 18S rDNA. <b><i>Results:</i></b> These hospitals did not record cases of human babesiosis, although several cases of febrile illnesses and malaria were reported. The blood of 326 animals tested positive for <i>Babesia</i> DNA. Thus, the overall prevalence of <i>Babesia</i> infection in sampled animals was 80.69% (326/404), with infection prevalence varying among the targeted reservoir hosts: 53.07% (173/326) dogs, 3.99% (13/326) cats, and 42.9% (140/326) rodents. <b><i>Conclusion:</i></b> Although the hospitals documented no cases of human babesiosis, <i>Babesia</i> transmission was active among reservoir hosts. Therefore, clinicians and laboratory scientists in the Greater Accra region and Ghana should maintain a high index of suspicion of human babesiosis to avoid missing such cases.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of <i>Leptospira</i> Spp. in Nonhuman Primates and Wild Felids from a Brazilian Zoo.","authors":"Ana Cláudia de Souza Andrade, Francieli Gesleine Capote Bonato, Isabela Carvalho Dos Santos, Lídia Kazue Iukava, Sarah Gabriella Delallo Charnovski, Bruna Pereira Bitencourt da Silva, Edilson Monteiro Suci Junior, Lucienne Garcia Pretto-Giordano, Ranulfo Piau Junior, Rodrigo Neca Ribeiro, Zilda Cristiani Gazim, Gabriela Catuzo Canonico Silva, Lidiane Nunes Barbosa, Daniela Dib Gonçalves","doi":"10.1089/vbz.2025.0020","DOIUrl":"https://doi.org/10.1089/vbz.2025.0020","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Leptospirosis is a zoonotic disease caused by the bacterium Leptospira spp., which is divided into saprophytic and pathogenic. Transmission in zoos can occur due to infected urine in poorly sanitized environments, rodents, and animal accumulation. Aim: This study investigated the presence of antibodies and DNA of Leptospira spp. in nonhuman primates and wild felids from a zoo in western Paraná, Brazil. <b><i>Materials and Methods:</i></b> Samples were collected through chemical restraint and venipuncture. Antibody testing was performed by microscopic agglutination test and polymerase chain reaction (PCR). <b><i>Results:</i></b> Of the 24 samples analyzed (17 capuchin monkeys and 7 felines), 12.5% presented antibodies for the Butembo serovar, all in primates. No feline showed reaction or DNA amplification. <b><i>Conclusion:</i></b> The results highlight the importance of studies on leptospirosis in wild animals in captivity, considering factors such as biome and management of the zoo, which houses rescued species that cannot be reintroduced into the wild.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Borrelia miyamotoi</i> in <i>Ixodes ricinus</i> Ticks Removed from Hosts in Northern Italy.","authors":"Emanuela Olivieri, Sara Rigamonti, Nadia Vicari, Andrea Grassi, Iyad Karaman, Irene Bertoletti, Alessandro Bianchi, Claudio Marco Lodola, Giuseppe Polonini, Giulia Maioli, Paola Prati","doi":"10.1089/vbz.2025.0017","DOIUrl":"https://doi.org/10.1089/vbz.2025.0017","url":null,"abstract":"<p><p><i>Borrelia miyamotoi</i> is a relapsing fever spirochete, first identified in Japan and later detected in various <i>Ixodes</i> species across the northern hemisphere. The pathogen was linked to human infections in North America, Europe, Asia, and Russia, causing symptoms ranging from flu-like illness to severe neurological damage. This study aimed to assess the presence of <i>B. miyamotoi</i> in <i>Ixodes ricinus</i> ticks collected from wildlife, dogs, and humans in Lombardy, Northern Italy, between 2020 and 2023. A total of 3,886 ticks were sampled, with a 1% positivity rate. The spirochete was detected in all life stages of ticks, with sequences 100% identical to European strains. The pathogen was found mostly in northern mountain regions. Although no human cases have been reported in Italy, these areas pose a potential risk due to high tourist activity.</p>","PeriodicalId":23683,"journal":{"name":"Vector borne and zoonotic diseases","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}