Veterinarni Medicina最新文献_第5页

Epidemiological insights into bovine helminthiasis in Upper Egypt: Prevalence, risk factors, and pathological findings. 上埃及牛蠕虫病的流行病学见解：患病率、危险因素和病理结果。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-10-31 eCollection Date: 2025-10-01 DOI: 10.17221/112/2024-VETMED

Abeer Ali Khedr, Sherief Mohamed Abdel-Raheem, Mohsen Mohamed Farghaly, Saad Ibrahim Alsultan, Mohamad Abdulmohsen, Sayed Fathi El-Hawari, Wafaa Gamal El-Dein Mahmoud

{"title":"Epidemiological insights into bovine helminthiasis in Upper Egypt: Prevalence, risk factors, and pathological findings.","authors":"Abeer Ali Khedr, Sherief Mohamed Abdel-Raheem, Mohsen Mohamed Farghaly, Saad Ibrahim Alsultan, Mohamad Abdulmohsen, Sayed Fathi El-Hawari, Wafaa Gamal El-Dein Mahmoud","doi":"10.17221/112/2024-VETMED","DOIUrl":"10.17221/112/2024-VETMED","url":null,"abstract":"This study examines the prevalence of helminthic infestations, risk factors, and odds ratios in 1 300 cattle, revealing significant patterns in parasite distribution and influencing factors. Overall, 60.3% of cattle were infested with one or more types of parasites, with Moniezia spp. being the most prevalent (46.9%), followed by Fasciola spp. (36.9%), Paramphistomum spp. (26.8%), and Avitellina spp. (10.8%). Among the seasons, winter exhibited the highest infestation rate (66.1%), and calves under one year of age were more commonly infested (64.2%) than older cattle (over three years: 51.1%, OR = 0.584 1). Distinct seasonal and age-related patterns were observed for specific parasites. Fasciola spp. was most prevalent in winter (45.09%) and among the youngest cattle (47.87%), while Paramphistomum spp. and Moniezia spp. showed less variation across seasons. Avitellina spp. had the lowest infestation rates in the spring, and cattle older than three years were affected. Co-infections were common, notably between Fasciola and other parasites, with the highest co-infestation rate observed between Avitellina spp. and Moniezia spp. Analysis of deworming efficacy indicated higher treatment success for Fasciola spp. and Paramphistomum spp., lower odds of response for Avitellina spp., and no significant treatment effect for Moniezia spp. Microscopic and pathological examinations were also conducted. These results demonstrate the necessity of thorough deworming procedures along with targeted parasite control to reduce significant health hazards in cattle populations.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 10","pages":"339-357"},"PeriodicalIF":0.8,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12666621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of zinc oxide nanoparticles on the oxidative stress, caspase-3, cytokine and immunity in rats. 氧化锌纳米颗粒对大鼠氧化应激、caspase-3、细胞因子及免疫的影响。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-10-30 eCollection Date: 2025-10-01 DOI: 10.17221/11/2025-VETMED

Meryem Gultekin, Meryem Eren, Fatih Dogan Koca, Caglar Kaan Bozbek, Nermin Develi

{"title":"The effects of zinc oxide nanoparticles on the oxidative stress, caspase-3, cytokine and immunity in rats.","authors":"Meryem Gultekin, Meryem Eren, Fatih Dogan Koca, Caglar Kaan Bozbek, Nermin Develi","doi":"10.17221/11/2025-VETMED","DOIUrl":"10.17221/11/2025-VETMED","url":null,"abstract":"This study was conducted to examine the effects of zinc oxide nanoparticles (ZnO NPs) on the malondialdehyde (MDA) concentrations, superoxidase dismutase (SOD), glutathione peroxidase (GPx) and caspase-3 (CASP3) activities, tumour necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), immunoglobulin (Ig) E, G, M and zinc (Zn) concentrations in the serum/plasma and liver tissues of rats. Forty Wistar Albino rats were separated into five equal groups as the control, 5 and 10 mg/kg, b.w./day ZnO, 5 and 10 mg/kg b.w./day ZnO NPs were administered i.p. every other day for 14 days. The plasma MDA and plasma/liver TNF-α concentrations increased in the 10 mg/kg ZnO, 5 and 10 mg/kg ZnO NPs groups. The plasma SOD, CASP3, plasma/liver GPx activities and serum Zn concentrations increased in all the Zn groups. The highest SOD, GPx and CASP3 activities were detected in the 5 mg/kg ZnO NPs group. The plasma IgG concentrations increased, especially in the ZnO NPs groups. The study findings suggest that 5 mg/kg ZnO NPs could potentially have an ameliorative effect on the possible adverse effects of oxidative stress. These nanoparticles demonstrate their ability to combat oxidative stress by increasing the plasma/tissue SOD, GPx, and CASP3 activities, TNF-α, and IgG concentrations. However, the effectiveness of the nanoparticles may vary depending on the synthesis method, application time, and concentration.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 10","pages":"379-392"},"PeriodicalIF":0.8,"publicationDate":"2025-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12666622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of lumbosacral transitional vertebrae and association with hip dysplasia in Rhodesian Ridgebacks in Germany. 腰骶过渡椎骨的患病率和与髋发育不良在德国罗得西亚背。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-10-29 eCollection Date: 2025-10-01 DOI: 10.17221/30/2025-VETMED

Svenja Kristiane Slunsky, Pavel Slunsky, Emanuel Fort, Leo Brunnberg

{"title":"Prevalence of lumbosacral transitional vertebrae and association with hip dysplasia in Rhodesian Ridgebacks in Germany.","authors":"Svenja Kristiane Slunsky, Pavel Slunsky, Emanuel Fort, Leo Brunnberg","doi":"10.17221/30/2025-VETMED","DOIUrl":"10.17221/30/2025-VETMED","url":null,"abstract":"Congenital anomalies affecting the spinal column are frequently observed in certain dog breeds. One such condition occurs at the junction between the lower back and the sacrum, where vertebrae exhibit mixed structural characteristics. This retrospective study analysed imaging records of Rhodesian Ridgebacks collected over 28 years, selecting only those that met strict positioning standards for evaluation. A total of 2 012 cases were analysed using established classification methods, identifying three distinct structural variations: a typical junction, a bilaterally altered morphology, and an asymmetrical form affecting one side. The prevalence of these variations was 7.4%, with symmetrical alterations found in 5% of cases and asymmetrical alterations in 2.4%. Statistical analysis did not reveal a significant association between these anatomical variations and canine hip dysplasia (CHD) (P = 0.170 0). These findings support previous research indicating that there is no direct link between these structural variations and joint disorders in this breed. The study represents the most extensive investigation of its kind in this canine population, highlighting that such vertebral variations are frequently observed in routine radiographic screenings, but despite decades of research, consistent clinical correlations remain elusive - underscoring the need for further systematic investigation.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 10","pages":"369-378"},"PeriodicalIF":0.8,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12666619/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of trace mineral injection on serum trace mineral concentrations and reproductive indicators in sheep synchronised during the anoestrus period. 微量元素注射对绵羊发情期同步血清微量元素浓度和生殖指标的影响。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-10-29 eCollection Date: 2025-10-01 DOI: 10.17221/28/2025-VETMED

Atakan Cortu, Orsan Gungor

{"title":"Effect of trace mineral injection on serum trace mineral concentrations and reproductive indicators in sheep synchronised during the anoestrus period.","authors":"Atakan Cortu, Orsan Gungor","doi":"10.17221/28/2025-VETMED","DOIUrl":"10.17221/28/2025-VETMED","url":null,"abstract":"Sheep production represents an important source of income for farmers in Türkiye; however, reproductive inefficiencies during the anoestrus period pose a major challenge to productivity. This study aimed to evaluate the effects of injectable trace minerals (copper, selenium, manganese, and zinc) on the reproductive performance of Awassi ewes synchronised during the anoestrous period. A total of 200 clinically healthy ewes were randomly allocated to two groups: the trace mineral group (TRACE, n = 100), which received 2 ml of a trace mineral solution 14 days before oestrus synchronisation, and the control group (CON, n = 100), which received 2 ml of 0.9% NaCl. Oestrus was synchronised using intravaginal sponges containing 60 mg medroxyprogesterone acetate for 12 days, followed by administration of 500 IU equine chorionic gonadotrophin at sponge removal. Ewes were hand-mated for five days following synchronisation. Variables assessed included serum trace mineral concentrations, response to synchronisation, and reproductive outcomes (oestrus rate, pregnancy rate, lambing rate, incidence of multiple births, and dystocia). No significant differences (P > 0.05) were found between groups in serum concentrations of copper, zinc, or manganese. Although the duration of oestrus was significantly longer in the TRACE group compared to control (29.66 ± 0.96 h vs 26.09 ± 0.89 h; P = 0.006), all other reproductive indicators were similar between groups. These findings suggest that a single pre-synchronisation injection of trace minerals does not significantly improve the reproductive performance or mineral status in anoestrous Awassi ewes. Further research is needed to determine the optimal timing and dosing strategies for supplementing trace minerals in sheep.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 10","pages":"358-368"},"PeriodicalIF":0.8,"publicationDate":"2025-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12666620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145661199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Far lateral lumbar intervertebral disc extrusion in a cat: Case report. 猫远侧侧腰椎间盘突出：1例报告。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-09-30 eCollection Date: 2025-09-01 DOI: 10.17221/107/2024-VETMED

Youjung Jang, Hyung-Kyu Chae, Yeon-Jung Hong

引用次数: 0

Influence of dietary coriander seeds and administration of Lactobacillus acidophilus on the performance of growing rabbits under subtropical climatic conditions. 亚热带气候条件下饲粮添加香菜籽和嗜酸乳杆菌对生长兔生产性能的影响。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-09-29 eCollection Date: 2025-09-01 DOI: 10.17221/104/2024-VETMED

Sherief Mohamed Abdel-Raheem, Mustafa Ahmed Kobeisy, Yasmin Adel Abdel-Wadood Gomaa, Ahmed Meligy Abdelghany Meligy, Mahmoud Elalfy, Mohsen Mohamed Farghaly

{"title":"Influence of dietary coriander seeds and administration of Lactobacillus acidophilus on the performance of growing rabbits under subtropical climatic conditions.","authors":"Sherief Mohamed Abdel-Raheem, Mustafa Ahmed Kobeisy, Yasmin Adel Abdel-Wadood Gomaa, Ahmed Meligy Abdelghany Meligy, Mahmoud Elalfy, Mohsen Mohamed Farghaly","doi":"10.17221/104/2024-VETMED","DOIUrl":"10.17221/104/2024-VETMED","url":null,"abstract":"This study investigated the effects of coriander seed powder and Lactobacillus acidophilus solution (LAS) on the growth, nutrient digestibility, blood parameters, and intestinal health of growing rabbits under subtropical conditions. Forty Californian rabbits, aged 35 days with an average body weight of 588 ± 34 g, were randomly assigned to four groups: a control group fed a standard diet, a group (T1) receiving the basal diet supplemented with 1.5% coriander seed powder, a group (T2) receiving the basal diet with oral LAS at 1 × 109 CFU/kg, and a group (T3) receiving both coriander seed powder and LAS. The study measured body weight, daily weight gain, feed conversion ratio, mortality rate, blood metabolites, nutrient digestibility, and intestinal histomorphology. Results showed that rabbits fed with 1.5% coriander seed powder had significant improvements in body weight gain, feed conversion, and a reduction in mortality compared to the control. Both coriander seed powder and LAS improved blood metabolites, nutrient digestibility, and intestinal health. However, the combination of both additives did not provide additional benefits over the individual treatments. The findings suggest that either 1.5% coriander seed powder or LAS can enhance growth performance and health in rabbits under subtropical conditions.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 9","pages":"313-328"},"PeriodicalIF":0.8,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12539397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145347608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Blastocystis first detected in Sciurus vulgaris and Sciurus vulgaris exalbidus in Chengdu, China. 囊虫在成都地区首次检出。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-09-29 eCollection Date: 2025-09-01 DOI: 10.17221/71/2024-VETMED

Xiaobo Li, Haocheng Huang, Yifan Liu, Wanyu Meng, Zhijun Zhong, Ziyao Zhou, Guangneng Peng, Jianbao Han, Haifeng Liu

{"title":"Blastocystis first detected in Sciurus vulgaris and Sciurus vulgaris exalbidus in Chengdu, China.","authors":"Xiaobo Li, Haocheng Huang, Yifan Liu, Wanyu Meng, Zhijun Zhong, Ziyao Zhou, Guangneng Peng, Jianbao Han, Haifeng Liu","doi":"10.17221/71/2024-VETMED","DOIUrl":"10.17221/71/2024-VETMED","url":null,"abstract":"The anaerobic unicellular protist Blastocystis is widely recognised for its presence in the gastrointestinal systems of humans and various animals globally. However, there is a paucity of reports on the prevalence and subtype (ST) distribution of Blastocystis in the squirrel population. This study was conducted to determine the prevalence and genetic diversity of Blastocystis, as well as its zoonotic potential, among Sciurus vulgaris and Sciurus vulgaris exalbidus in Chengdu, China. A total of 41 faecal samples (31 from Sciurus vulgaris, 10 from Sciurus vulgaris exalbidus) were analysed for the presence of Blastocystis sp. using the polymerase chain reaction (PCR) amplification of the small subunit ribosomal RNA (SSU rRNA) gene. Our findings revealed a positive rate of 4.88% (2/41 samples) for Blastocystis sp., with both identified as ST4 through nucleotide sequence homology and phylogenetic analysis. Given the zoonotic nature of this subtype, farmed squirrels may serve as potential reservoirs for Blastocystis transmission to humans and domestic animals. These findings are essential for developing effective control strategies against Blastocystis in the study region and enhancing our comprehension of the genetic spectrum of Blastocystis within Sciurus vulgaris and Sciurus vulgaris exalbidus.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 9","pages":"329-334"},"PeriodicalIF":0.8,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12539398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145347643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-year study monitoring the mercury content in the tissues of cattle sampled in the Czech Republic between 2014 and 2023. 对2014年至2023年捷克共和国牛样本组织中汞含量的多年监测研究。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-09-26 eCollection Date: 2025-09-01 DOI: 10.17221/34/2025-VETMED

Martin Svoboda, Veronika Vlasakova, Danka Harustiakova, Josef Illek, Andrea Staffa, Jan Vasek, Kamila Novotna Kruzikova, Jana Cahova, Zdenka Svobodova

{"title":"Multi-year study monitoring the mercury content in the tissues of cattle sampled in the Czech Republic between 2014 and 2023.","authors":"Martin Svoboda, Veronika Vlasakova, Danka Harustiakova, Josef Illek, Andrea Staffa, Jan Vasek, Kamila Novotna Kruzikova, Jana Cahova, Zdenka Svobodova","doi":"10.17221/34/2025-VETMED","DOIUrl":"10.17221/34/2025-VETMED","url":null,"abstract":"Analyses of mercury concentrations in the muscle, liver and kidneys of cattle were conducted in the Czech Republic during the period from 2014 to 2023. The average mercury content in muscles, livers, and kidneys of calves was 0.000 5 ± 0.000 0 mg.kg-1, 0.002 7 ± 0.000 5 mg.kg-1, and 0.004 1 ± 0.000 8 mg.kg-1, respectively. In fattening cattle, the average mercury content in muscles, livers and kidneys was 0.000 5 ± 0.000 0 mg.kg-1, 0.002 1 ± 0.000 2 mg.kg-1 and 0.004 9 ± 0.000 3 mg.kg-1, respectively. In cows, the average mercury content in muscles, livers and kidneys was 0.000 5 ± 0.000 0 mg.kg-1, 0.002 3 ± 0.000 1 mg.kg-1 and 0.006 9 ± 0.000 3 mg.kg-1, respectively. The maximum residual limit for human consumption was exceeded in 10 kidney samples (3 calves, 6 cows, 1 fattening cattle) and 1 liver sample (calf). In all age categories, the highest mercury concentrations were found in the kidneys, lower in the livers, and the lowest in the muscles. When comparing the age groups, significantly higher mercury concentrations were observed in the kidneys of cows than in calves and fattening cattle. It can be concluded that there is still a need for further monitoring of mercury concentrations in cattle tissues in the Czech Republic.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 9","pages":"307-312"},"PeriodicalIF":0.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12539396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145347584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SOD1 and CAT gene expressions in stray and owned animals. 流浪动物和自养动物SOD1和CAT基因表达。

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-08-30 eCollection Date: 2025-08-01 DOI: 10.17221/12/2025-VETMED

Gonca Sonmez, Mehmet Cevat Temizkan, Muhammed Hudai Culha

{"title":"SOD1 and CAT gene expressions in stray and owned animals.","authors":"Gonca Sonmez, Mehmet Cevat Temizkan, Muhammed Hudai Culha","doi":"10.17221/12/2025-VETMED","DOIUrl":"10.17221/12/2025-VETMED","url":null,"abstract":"Dogs and cats are the most common companion animals in urban environments. Some dogs and cats live in stable home settings, receiving regular care. However, stray dogs and cats are exposed to chronic stress due to life on the streets or in shelters. Stress is correlated with oxidative stress. The dysregulation of redox balance can lead to the accumulation of reactive oxygen species, which promote cellular and molecular damage. In this study, the blood samples were collected from 150 female animals (90 dogs and 60 cats) to isolate peripheral blood mononuclear cells (PBMCs). Total RNA was extracted from PBMCs and subjected to reverse transcription. The expression levels of SOD1 and CAT were quantified using quantitative real-time polymerase chain reaction. Compared with those in stray animals, the SOD1 expression levels were significantly higher (P < 0.01), and the CAT expression levels were non-significantly lower in owned animals. These findings suggest that the expression of antioxidant defence genes varied between owned and stray animals. Thus, oxidative stress regulation is dependent on environmental conditions and lifestyle in companion species.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 8","pages":"273-278"},"PeriodicalIF":0.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic and apoptotic effects of etoposide and ellagic acid alone or with possible synergistic/additive combinations on a canine D-17 osteosarcoma cell line. 依托泊苷和鞣花酸单独或可能协同/添加剂组合对犬D-17骨肉瘤细胞系的细胞毒性和凋亡作用

IF 0.8 4区农林科学

Veterinarni Medicina Pub Date : 2025-08-30 eCollection Date: 2025-08-01 DOI: 10.17221/24/2025-VETMED

Gamze Sevri Ekren Asici, Funda Kiral, Aysegul Bildik, Pinar Alkim Ulutas

{"title":"Cytotoxic and apoptotic effects of etoposide and ellagic acid alone or with possible synergistic/additive combinations on a canine D-17 osteosarcoma cell line.","authors":"Gamze Sevri Ekren Asici, Funda Kiral, Aysegul Bildik, Pinar Alkim Ulutas","doi":"10.17221/24/2025-VETMED","DOIUrl":"10.17221/24/2025-VETMED","url":null,"abstract":"Osteosarcoma (OSA) is the most prevalent and aggressive malignancy of canine bones. Etoposide is an effective chemotherapeutic agent for cancer treatment, whereas ellagic acid is a naturally occurring compound with antioxidant and anticancer properties; however, both are inhibitors of the topoisomerase enzyme. In this study, the synergistic/additive effect of etoposide, known to have a growth-inhibitory impact in canine osteosarcoma (OSA) cells, and ellagic acid was investigated. The mechanism by which this effect inhibits cell growth at lower etoposide doses was also examined. The IC50 values of both agents were determined, and possible combination doses were generated accordingly and applied to canine OSA cells. The apoptotic effects of the combinations were evaluated based on DNA breaks and the activity levels of caspase 3, 8, and 9. These findings were supported by the expression levels of Bcl-2, Bax, and Bid genes, as well as the AO/EtBr staining method. The effects on cell cycle and proliferation were analysed through survivin and NF-κβ gene expressions. Antimetastatic effects were determined using invasion and migration assays. EA is a potential therapeutic agent for cancer treatment. In combination with ET, a higher anticancer efficacy was demonstrated compared to etoposide alone. Potential treatment side effects can be reduced by enabling the use of lower drug doses.","PeriodicalId":23532,"journal":{"name":"Veterinarni Medicina","volume":"70 8","pages":"279-293"},"PeriodicalIF":0.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12456270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0