Tissue Barriers最新文献_第4页

(Zebra)fishing for nephrogenesis genes. (斑马）寻找肾脏生成基因。

IF 3.6

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-05-31 DOI: 10.1080/21688370.2023.2219605

Brooke E Chambers, Nicole E Weaver, Caroline M Lara, Thanh Khoa Nguyen, Rebecca A Wingert

引用次数: 0

Exosomes: current knowledge and future perspectives. 外泌体：现有知识和未来展望。

IF 3.6

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-07-13 DOI: 10.1080/21688370.2023.2232248

Swati Singh, Deepraj Paul, Virendra Nath, Rohini A

{"title":"Exosomes: current knowledge and future perspectives.","authors":"Swati Singh, Deepraj Paul, Virendra Nath, Rohini A","doi":"10.1080/21688370.2023.2232248","DOIUrl":"10.1080/21688370.2023.2232248","url":null,"abstract":"Exosomes are membrane-bound micro-vesicles that possess endless therapeutic potential for treatment of numerous pathologies including autoimmune, cardiovascular, ocular, and nervous disorders. Despite considerable knowledge about exosome biogenesis and secretion, still, there is a lack of information regarding exosome uptake by cell types and internal signaling pathways through which these exosomes process cellular response. Exosomes are key components of cell signaling and intercellular communication. In central nervous system (CNS), exosomes can penetrate BBB and maintain homeostasis by myelin sheath regulation and the waste products elimination. Therefore, the current review summarizes role of exosomes and their use as biomarkers in cardiovascular, nervous and ocular disorders. This aspect of exosomes provides positive hope to monitor disease development and enable early diagnosis and treatment optimization. In this review, we have summarized recent findings on physiological and therapeutic effects of exosomes and also attempt to provide insights about stress-preconditioned exosomes and stem cell-derived exosomes.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11042064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9776715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblasts/three-dimensional scaffolds complexes promote wound healing in rats with skin defects. 成纤维细胞/三维支架复合物可促进皮肤缺损大鼠的伤口愈合。

IF 3.1

Tissue Barriers Pub Date : 2024-03-27 DOI: 10.1080/21688370.2024.2334544

Ting Jiang, Qiang Liu, Er-Chang Xu, Si-Yu He, Hong-Yan Liu, Chao Tian, Lan-Fang Zhang, Ze-Long Yang

{"title":"Fibroblasts/three-dimensional scaffolds complexes promote wound healing in rats with skin defects.","authors":"Ting Jiang, Qiang Liu, Er-Chang Xu, Si-Yu He, Hong-Yan Liu, Chao Tian, Lan-Fang Zhang, Ze-Long Yang","doi":"10.1080/21688370.2024.2334544","DOIUrl":"https://doi.org/10.1080/21688370.2024.2334544","url":null,"abstract":"We aim to construct a three-dimensional nano-skin scaffold material in vitro and study its promoting effect on wound healing in vivo. In this study, hybrid constructs of three-dimensional (3D) scaffolds were successfully fabricated by combination of type I collagen (COL-1) and polylactic-glycolic acid (PLGA). Fibroblasts and human umbilical cord mesenchymal stem cells (hUCMSCs) were used to implanted into 3D scaffolds and constructed into SD skin scaffolds in vitro. Finally, the fibroblasts/scaffolds complexes were inoculated on the surface of rat wound skin to study the promoting effect of the complex on wound healing. In our study, we successfully built a 3D scaffold, which had a certain porosity. Meanwhile, the content of COL-1 in the cell supernatant of fibroblast/scaffold complexes was increased. Furthermore, the expression of F-actin, CD105, integrin β, VEGF, and COL-1 was up-regulated in hUCMSC/scaffold complexes compared with the control group. In vivo, fibroblast/scaffold complexes promoted wound healing in rats. Our data suggested that the collagen Ⅳ and vimentin were elevated and collagen fibers were neatly arranged in the fibroblast/scaffold complex group was significantly higher than that in the scaffold group. Taken together, fibroblast/scaffold complexes were expected to be novel materials for treating skin defects.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140307054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inter-claudin antagonism of paracellular pore function: mechanism and beyond. 细胞旁孔功能的克隆蛋白间拮抗作用：机制与超越。

IF 3.1

Tissue Barriers Pub Date : 2024-03-17 DOI: 10.1080/21688370.2024.2330773

Wanapas Wachiradejkul, Pawin Pongkorpsakol

引用次数: 0

1,25(OH)2D3 supplementation alleviates gut-vascular barrier disruption via inhibition of S100B/ADAM10 pathway. 通过抑制 S100B/ADAM10 通路补充 1,25(OH)2D3，减轻肠道血管屏障的破坏。

IF 3.1

Tissue Barriers Pub Date : 2024-03-17 DOI: 10.1080/21688370.2024.2327776

Aiwen Feng, Cheng Li, Shaosheng Su, Yingyan Liu

{"title":"1,25(OH)2D3 supplementation alleviates gut-vascular barrier disruption via inhibition of S100B/ADAM10 pathway.","authors":"Aiwen Feng, Cheng Li, Shaosheng Su, Yingyan Liu","doi":"10.1080/21688370.2024.2327776","DOIUrl":"https://doi.org/10.1080/21688370.2024.2327776","url":null,"abstract":"Gut-vascular barrier (GVB) is the second barrier in mucosa to control systemic dissemination of gut bacteria. Severe burns induce enteroglial cells to produce S100B and endothelial cells to generate ADAM10 and cause vitamin D3 insufficiency/deficiency and GVB disruption. It is not clear whether vitamin D3 supplementation attenuates GVB damage via regulation of S100B/ADAM10 pathway. Here, GVB disruption was induced by 30% of total body surface area scalds. Rats were treated with 1,25(OH)2D3 (0.05, 0.5 or 5 μg/kg) or S100B monoclonal antibody (S100BmAb, 10 μg/kg) or GI254023X (ADAM10 inhibitor, 100 mg/kg). Rat enteric glial cell-line CRL2690 and rat intestinal microvascular endothelial cells (RIMECs) were treated with S100B (5 μM) or plus 1,25(OH)2D3 (0.05, 0.5 or 5 μM) or GI254023X (5 μM). S100B, TNF-α, 25(OH)D3 and 1,25(OH)2D3 in serum and gut mucosa were determined by enzyme-linked immunosorbent assay. The endothelial permeability was measured using FITC-dextran 70 kDa. ADAM10 and β-catenin expression was assayed by Western blot. The results showed that 1,25(OH)2D3 and 25(OH)D3 concentration in serum reduced whereas TNF-α and S100B in serum and gut mucosa increased in burned rats. S100BmAb, GI254023X and 1,25(OH)2D3 treatment lowered burns-increased GVB permeability. 1,25(OH)2D3 also decreased S100B concentration in serum and gut mucosa. 1,25(OH)2D3 inhibited S100B release from TNF-α-treated CRL2690 and raised β-catenin while decreasing ADAM10 protein in S100B-treated RIMECs. 1,25(OH)2D3 and GI254023X also decreased the endothelial permeability of S100B-treated RIMECs. Collectively, these findings provide evidence that severe burns lower serum 25(OH)D3 and 1,25(OH)2D3 concentration. 1,25(OH)2D3 supplementation alleviates burns-elicited GVB disruption via inhibition of S100B/ADAM10 signaling.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140144149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly scalable and standardized organ-on-chip platform with TEER for biological barrier modeling. 具有高度可扩展性和标准化的片上器官平台，可利用 TEER 进行生物屏障建模。

IF 3.1

Tissue Barriers Pub Date : 2024-02-12 DOI: 10.1080/21688370.2024.2315702

Hoang-Tuan Nguyen, Siiri-Liisa Rissanen, Mimosa Peltokangas, Tino Laakkonen, Jere Kettunen, Lara Barthod, Ragul Sivakumar, Anniina Palojärvi, Pauliina Junttila, Jussi Talvitie, Michele Bassis, Sarah L Nickels, Sara Kalvala, Polina Ilina, Päivi Tammela, Sarka Lehtonen, Jens C Schwamborn, Sebastien Mosser, Prateek Singh

{"title":"Highly scalable and standardized organ-on-chip platform with TEER for biological barrier modeling.","authors":"Hoang-Tuan Nguyen, Siiri-Liisa Rissanen, Mimosa Peltokangas, Tino Laakkonen, Jere Kettunen, Lara Barthod, Ragul Sivakumar, Anniina Palojärvi, Pauliina Junttila, Jussi Talvitie, Michele Bassis, Sarah L Nickels, Sara Kalvala, Polina Ilina, Päivi Tammela, Sarka Lehtonen, Jens C Schwamborn, Sebastien Mosser, Prateek Singh","doi":"10.1080/21688370.2024.2315702","DOIUrl":"https://doi.org/10.1080/21688370.2024.2315702","url":null,"abstract":"The development of new therapies is hampered by the lack of predictive, and patient-relevant in vitro models. Organ-on-chip (OOC) technologies can potentially recreate physiological features and hold great promise for tissue and disease modeling. However, the non-standardized design of these chips and perfusion control systems has been a barrier to quantitative high-throughput screening (HTS). Here we present a scalable OOC microfluidic platform for applied kinetic in vitro assays (AKITA) that is applicable for high, medium, and low throughput. Its standard 96-well plate and 384-well plate layouts ensure compatibility with existing laboratory workflows and high-throughput data collection and analysis tools. The AKITA plate is optimized for the modeling of vascularized biological barriers, primarily the blood-brain barrier, skin, and lung, with precise flow control on a custom rocker. The integration of trans-epithelial electrical resistance (TEER) sensors allows rapid and repeated monitoring of barrier integrity over long time periods. Together with automated liquid handling and compound permeability testing analyses, we demonstrate the flexibility of the AKITA platform for establishing human-relevant models for preclinical drug and precision medicine's efficacy, toxicity, and permeability under near-physiological conditions.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139724198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One small step for stool, one giant leap for IBD surveillance. 粪便的一小步，IBD 监测的一大步。

IF 3.1

Tissue Barriers Pub Date : 2024-02-07 DOI: 10.1080/21688370.2024.2314839

Dorrian G Cohen, Rebecca A Wingert

引用次数: 0

Slow down my beating heart: induction of cardiac fibrosis by Iroquois homeobox 2. 让我跳动的心脏慢下来：Iroquois homeobox 2诱导心脏纤维化。

IF 3.1

Tissue Barriers Pub Date : 2024-01-28 DOI: 10.1080/21688370.2024.2309036

Madeline Petrikas, Rebecca A Wingert

引用次数: 0

Tails of nephron ciliated cell development: insights on patterning a functional tissue barrier from the zebrafish. 肾小管纤毛细胞发育的尾巴：斑马鱼功能性组织屏障模式化的启示。