Ukrainian Biochemical Journal最新文献_第3页

A new view of RNA: the 1989 discovery by Sidney Altman and Thomas Cech RNA的新观点：1989年西德尼·奥特曼和托马斯·切赫的发现

Ukrainian Biochemical Journal Pub Date : 2020-11-16 DOI: 10.15407/ubj92.05.155

M. Grigorieva, V. M. Danilova, S. Komisarenko

引用次数: 1

A glance on the role of Hsien Wu in immunology development 仙武在免疫学发展中的作用概述

Ukrainian Biochemical Journal Pub Date : 2020-11-16 DOI: 10.15407/ubj92.05.161

M. Ebrahimi

引用次数: 0

Research on structure, mechanism and regulation of enzyme activity. Works of Nobel laureates C. Anfinsen, S. Moore, W. Stein, S. Prusiner, J. Skou, P. Boyer, J. Walker 酶活性的结构、机制及调控研究。诺贝尔奖获得者C.安芬森、S.摩尔、W.斯坦、S.普鲁西纳、J.斯库、P.博伊尔、J.沃克的作品

Ukrainian Biochemical Journal Pub Date : 2020-11-16 DOI: 10.15407/ubj92.05.134

R. P. Vynogradova, V. M. Danilova, S. Komisarenko

引用次数: 0

Changes in gene expression of lactate carriers (MCT1 and CD147) in cardiac muscle of diabetic male rats: the effect of dichloroacetate and endurance training 糖尿病雄性大鼠心肌乳酸载体MCT1和CD147基因表达的变化:二氯乙酸和耐力训练的影响

Ukrainian Biochemical Journal Pub Date : 2020-11-16 DOI: 10.15407/ubj92.05.111

H. Rezaeinasab, A. Habibi, M. Nikbakht, M. Rashno, S. Shakerian

{"title":"Changes in gene expression of lactate carriers (MCT1 and CD147) in cardiac muscle of diabetic male rats: the effect of dichloroacetate and endurance training","authors":"H. Rezaeinasab, A. Habibi, M. Nikbakht, M. Rashno, S. Shakerian","doi":"10.15407/ubj92.05.111","DOIUrl":"https://doi.org/10.15407/ubj92.05.111","url":null,"abstract":"lactate accumulation can activate the pathways of mitochondrial biogenesis in the heart muscle. the purpose of this study was to investigate the effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) inhibition and endurance training on the gene expression of lactate carriers (MCT1 and CD147) in the cardiac muscle of Stz-diabetic rats. In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after induction of diabetes with streptozotocin (STZ). The endurance training protocol was performed on a treadmill for 6 weeks. Intraperitoneal injection of DCa of 50 mg/ kg body weight was used for the inhibition of PDk4 in the myocardium. Gene expression were measured using real-time PCR. the two-way aNoVa test was used to analyze the data. the results of the study showed that after endurance training, the expression of MCT1, PDK4, and CD147 genes increased significantly in line with each other (P < 0.05), and by inhibition of PDK4 in the heart muscle, the expression of MCT1 and CD147 genes in the endurance training group + diabetes + DCA and in the diabetes group + DCA decreased significantly (P < 0.05). According to the results of this study, it can be concluded that the repeated accumulation of lactate caused by exercise training in diabetic patients decrease through mitochondrial adaptation by DCa injection and subsequently oxidative stress can be reduced in cardiac tissue of diabetic patients and heart efficacy can be increased.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"111-119"},"PeriodicalIF":0.0,"publicationDate":"2020-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43817297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metallothioneins contribution to the response of bivalve mollusk to xenobiotics 金属硫蛋白在双壳类软体动物对外来生物的反应中的作用

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.087

V. Khoma, L. Gnatyshyna, V. Martyniuk

{"title":"Metallothioneins contribution to the response of bivalve mollusk to xenobiotics","authors":"V. Khoma, L. Gnatyshyna, V. Martyniuk","doi":"10.15407/ubj92.05.087","DOIUrl":"https://doi.org/10.15407/ubj92.05.087","url":null,"abstract":"Estimation of cellular thiols metallothioneins (mts) sensitivity to continuous pressure of environmental chemical ‘cocktail’ of xenobiotics needs investigation in correct model experiments. the aim of this study was to elucidate mts contribution into bivalve mollusk response to co-exposure to xenobiotics and elevated temperature. We treated the mussels Unio tumidus Philipson, 1788 (Unionidae) with drugs diclofenac (Dc, 2 nm), nifedipine (Nf, 2 nm) or with organophosphonate herbicide glyphosate (Gl, formulation roundup maX, 79 nm) separately at 18°c and in combination at 18°c (DcNfGl) and 25°c (DcNfGl+t) during 14 days. mts were isolated from digestive gland by size-exclusion chromatography. the concentration of mts in the tissue was assessed according to metals (Zn, cu, cd) in the eluted peak of mts (mt-me) and thiols (mt-Sh) content. tissue redox status was assessed using lactate/pyruvate ratio. the assay of cells viability was based on the lysosomes ability of hemocytes to concentrate the Neutral red (Nr) dye. It was found, that mt-Sh content in the digestive gland was increased under all exposures. treatment with Dc increased the level of mt-me, whereas treatment with Gl decreased it and increased lactate/pyruvate ratio. Nf decreased this ratio by elevating pyruvate level and increased lysosomal membrane stability in hemocytes. at co-exposure to xenobiotics and elevated temperature the number of hemocytes with nuclear abnormalities was increased indicating the exceeding of organisms’ adaptive limits. multivariate statistical analyses showed negative correlations in pairs mt-Sh/mt-me and mt-Sh/pyruvate and distinguished Gl and DcNfGl+t exposed groups from other groups.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"87-96"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45277805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Selected 5-amino-1-aryl-1H-1,2,3-triazole scaffolds as promising antiproliferative agents 选择5-氨基-1-芳基-1H-1,2,3-三唑支架作为有前景的抗增殖剂

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.023

N. Pokhodylo, O. Shyyka, N. Finiuk, R. Stoika

{"title":"Selected 5-amino-1-aryl-1H-1,2,3-triazole scaffolds as promising antiproliferative agents","authors":"N. Pokhodylo, O. Shyyka, N. Finiuk, R. Stoika","doi":"10.15407/ubj92.05.023","DOIUrl":"https://doi.org/10.15407/ubj92.05.023","url":null,"abstract":"Development of new effective drugs with low side effects and definite chemical characteristics needs identification of bioactive scaffolds for further structural optimization. New synthesized derivatives of 4-hetaryl-5-amino-1-aryl-1H-1,2,3-triazoles and 3H-[1,2,3]triazolo[4,5-b]pyridines were tested for anticancer activity using 60 human tumor cell lines within 9 cancer types. The selective influence of (5-amino-1H1,2,3-triazol-4-yl)quinazolin-4(3H)-ones: 2-(5-amino-1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl)quinazolin4(3H)-one and 2-(5-amino-1-phenyl-1H-1,2,3-triazol-4-yl)-6-bromoquinazolin-4(3H)-one on ovarian cancer OVCAR-4 cells with growth percentage (GP) = -4.08 and 6.63%, respectively, was found. The derivative 5,7-diamino-3-(3-(trifluoromethyl)phenyl)-3H-[1,2,3]triazolo[4,5-b]pyridine-6-carbonitrile possessed high activity towards lung cancer EKVX cells (GP = 29.14%). The compounds were shown to be less toxic than doxorubicin towards non-tumor human embryonic kidney cells of HEK293 line. Thus, the results of our study confirm the anticancer potential of compounds based on 5-amino-1-aryl-1H-1,2,3-triazoles scaffolds and their fused polycyclic derivatives.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"23-32"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43852858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals 低温保护剂影响实验动物淋巴器官和肝脏细胞中氨基酸与总蛋白的结合

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.041

A. K. Gulevskyy, Yu. S. Akhatova, A. Nikolchenko

引用次数: 0

ERN1 dependent regulation of TMED10, MYL9, SPOCK1, CUL4A and CUL4B genes expression at glucose and glutamine deprivations in U87 glioma cells ERN1依赖性调控U87胶质瘤细胞中葡萄糖和谷氨酰胺剥夺时TMED10、MYL9、SPOCK1、CUL4A和CUL4B基因的表达

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.050

O. Minchenko, O. Hnatiuk, D. O. Tsymbal

{"title":"ERN1 dependent regulation of TMED10, MYL9, SPOCK1, CUL4A and CUL4B genes expression at glucose and glutamine deprivations in U87 glioma cells","authors":"O. Minchenko, O. Hnatiuk, D. O. Tsymbal","doi":"10.15407/ubj92.05.050","DOIUrl":"https://doi.org/10.15407/ubj92.05.050","url":null,"abstract":"It was shown previously that inhibition of erN1 (endoplasmic reticulum to nucleus signaling 1) pathway, a central mediator of the unfolded protein response, leads to suppression of tumor growth through downregulation of key pro-proliferative and up-regulation of tumor suppressor factors and modifies the sensitivity of these genes to glucose and glutamine deprivation. However, the executive mechanisms of erN1 mediated control of glioma cell proliferation are not yet known. The goal of this study was to estimate the effect of glucose and glutamine deprivations on expression of cancer related genes in glioma U87 cells at erN1 signaling inhibition for evaluation of their possible significance in ERN1 mediated control of glioma cell proliferation. We studied the effect of glucose and glutamine deprivations on the expression level of cancer related genes encoding TMED10 (transmembrane p24 trafficking protein 10), MYL9 (myosin, light chain 9, regulatory), SPOck1 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1), cUl4a (cullin 4a), and cUl4B in U87 glioma control cells and cells with erN1 knockdown. It was shown that at glucose deprivation, the expression level of MYL9, SPOCK1 and CUL4B genes was significantly up-regulated in control glioma cells. ERN1 knockdown modified the sensitivity to glucose deprivation of all studied genes except TMED10 gene. At glutamine deprivation, the expression of Myl9, cUl4a and cUl4B genes was shown to be up-regulated in control glioma cells. the sensitivity of Myl9, tMeD10 and cUl4B gene expression to glutamine deprivation in glioma cells with ERN1 knockdown was significantly modified, while CUL4A and SPOCK1 gene expression did not respond to erN1 inhibition. the present study demonstrates that glucose and glutamine deprivation affected the expression of the most studied genes in a specific manner and that inhibition of ERN1 signaling preferentially modified their expression at glucose and glutamine deprivation.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"92 1","pages":"50-61"},"PeriodicalIF":0.0,"publicationDate":"2020-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48120180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver 亚磺酰亚乙基酯和铬（VI）对大鼠肝脏促/抗氧化系统状态的影响

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.078

B. Kotyk, R. Iskra, O. Slivinska, N. Liubas, A. Pylypets, V. Lubenets, V. I. Pryimych, U. Lviv

引用次数: 6

Dietary sucrose defines lifespan and metabolism in Drosophila 膳食蔗糖决定果蝇的寿命和新陈代谢

Ukrainian Biochemical Journal Pub Date : 2020-11-13 DOI: 10.15407/ubj92.05.097

O. Strilbytska, T. Strutynska, U. Semaniuk, N. Burdyliyk, O. Lushchak

引用次数: 6