Ukrainian Biochemical Journal最新文献_第2页

Indoleamine 2,3-dioxygenase level and oxidative stress parameters in the serum of patients with chronic renal failure 慢性肾功能衰竭患者血清吲哚胺2,3-双加氧酶水平及氧化应激参数

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.017

F. M. Y. Saeed, R. F. Jasim

引用次数: 0

Undecylenic acid and N,N-dibutylundecenamide as effective antibacterials against antibiotic-resistant strains 十一烯酸和N,N-二丁基十一烯酰胺作为抗抗生素耐药菌株的有效抗生素

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.055

Y. D. Startseva, D. M. Hodyna, I. V. Semenyuta, O. P. Tarasyuk, S. P. Rogalsky, L. O. Metelytsia

{"title":"Undecylenic acid and N,N-dibutylundecenamide as effective antibacterials against antibiotic-resistant strains","authors":"Y. D. Startseva, D. M. Hodyna, I. V. Semenyuta, O. P. Tarasyuk, S. P. Rogalsky, L. O. Metelytsia","doi":"10.15407/ubj95.04.055","DOIUrl":"https://doi.org/10.15407/ubj95.04.055","url":null,"abstract":"Evaluation of undecylenic acid (UA) and its tertiary amide N,N-dibutylundecenamide (DBUA) activity in vitro against the standard and antibiotic-resistant Escherichia coli and Staphylococcus aureus strains was carried out. The antibacterial potential of the acid and its amide at 2.5 and 5.0 μM concentration both against gram-positive bacteria (S. aureus) and gram-negative (E. coli) cultures was confirmed by monitoring the diameter of the bacterial growth inhibition zones. The docking study identified methionine aminopeptidase (MAP) as the most energy-favorable potential biotarget associated with the drug resistance of E. coli and S. aureus with a binding energy in the range from -8.0 to -8.5 kcal/mol. The ligands complexation was due to the formation of hydrogen bonds with ASP108, HIS171, HIS178, GLU204, GLU235, HIS76, ASP104, GLU233, ASP93 and metal-acceptor interactions with Co2+. Overall, the results indicated that UA and DBUA activity against antibiotic-resistant strains creates prospects for the development of new antibacterial formulations. Keywords: Escherichia coli, methionine aminopeptidase, molecular docking, Staphylococcus aureus, tertiary amide, undecylenic acid","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3D metals on α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium erubescens 三维金属中含有苹果果酸盐/锡酸盐阴离子和1,10-菲罗啉阳离子的配位化合物对延迟青霉、限制青霉和绿绿青霉α- l -鼠李糖苷酶活性的影响

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.046

O. V. Gudzenko, N. V. Borzova, L. D. Varbanets, I. I. Seifullina, O. E. Martsinko, E. V. Afanasenko

{"title":"The influence of coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3D metals on α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium erubescens","authors":"O. V. Gudzenko, N. V. Borzova, L. D. Varbanets, I. I. Seifullina, O. E. Martsinko, E. V. Afanasenko","doi":"10.15407/ubj95.04.046","DOIUrl":"https://doi.org/10.15407/ubj95.04.046","url":null,"abstract":"The search for effectors capable of influencing the catalytic activity of enzymes is an important area of modern enzymology. The aim of the study was to investigate the ability of 6 coordination compounds with malatogermanate/stannate anions and 1,10-phenanthroline cations of 3d metals to modify α-L-rhamnosidase activity of Penicillium tardum, Penicillium restrictum and Eupenicillium еrubescens strains. α-L-Rhamnosidase activity was determined by the Davis method using naringin as a substrate. It was demonstrated that [Ni(phen)3]2[{Sn(HMal)2(Mal)}Cl]•14H2O) in 0.1% concentration had the most pronounced activating effect on α-L-rhamnosidase activity of all strains studied. Noncompetitive inhibition of α-L-rhamnosidase in E. еrubescens by [Cu(phen)3]2[{Sn(HMal)2(Mal)}Cl]•10H2O was shown. The obtained results expand the idea of glycosidases possible activators and inhibitors and indicate the perspective of their use in modern biotechnological processes. Keywords: d-metals, double coordination compounds, Eupenicillium erubescens, germanium(IV), Penicillium restrictum, Penicillium tardum, stannum(IV), α-L-rhamnosidase","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats 2,6-二甲基吡啶n -氧化物(Ivin)对CCl(4)诱导的大鼠肝炎实验模型的肝保护作用

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.035

O. P. Vasetska, V. S. Lisovska, M. H. Prodanchuk, P. H. Zhminko

{"title":"Hepatoprotective effect of 2,6-dimethylpyridine N-oxide (Ivin) in experimental model of CCl(4)-induced hepatitis of rats","authors":"O. P. Vasetska, V. S. Lisovska, M. H. Prodanchuk, P. H. Zhminko","doi":"10.15407/ubj95.04.035","DOIUrl":"https://doi.org/10.15407/ubj95.04.035","url":null,"abstract":"The effect of concomitant pesticides and plant growth regulators on humans is still not well understood. N-oxide-2,6-dimethylpyridine (Ivin) is the plant growth regulator known to reduce the acute toxicity of pesticides, but its protective mechanisms need to be investigated. The aim of the study was to assess the hepatoprotective ability of 2,6-dimethylpyridine N-oxide (Ivin) using a model of acute CCl4-induced hepatitis. Male Wistar Han rats received two subcutaneous CCl4 injections (0.8 ml/100 g b.w.). Oral Ivin (13 or 0.13 mg/kg) and hepatoprotector “Silybor-35” (5 mg/kg) as reference substances were administered orally one hour pre- and 2 hours post-CCl4 injection. The biochemical assay of blood plasma, estimation of lipid peroxidation products in the liver tissue and histological liver analysis were done. The results of functional tests and histomorphological studies of liver tissue demonstrated that Ivin exhibited a pronounced hepatoprotective effect, more pronounced when it was administered at a low 0.13 mg/kg dose. Calculation of the hepatoprotection efficiency index for Ivin showed that it was comparable to that for “Silybor-35”. Keywords: hepatoprotective effect, Ivin, СCl4-induced hepatitis of rats","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135980452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ser-Thr phosphatases in the rat brain that dephosphorylate phospho-Ser(1291)-GluN2A subunit of glutamate receptor 大鼠脑中磷酸化磷酸化ser (1291)-GluN2A亚基的Ser-Thr磷酸酶

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.010

R. R. Prabhu

{"title":"Ser-Thr phosphatases in the rat brain that dephosphorylate phospho-Ser(1291)-GluN2A subunit of glutamate receptor","authors":"R. R. Prabhu","doi":"10.15407/ubj95.04.010","DOIUrl":"https://doi.org/10.15407/ubj95.04.010","url":null,"abstract":"N-methyl-D-aspartate receptors (NMDARs), are one of the major ionotropic glutamate receptors found in excitatory synapses which play a key role in glutamatergic synaptic transmission. The receptors are regulated by post translational modifications such as phosphorylation. One of the major receptor subunits is GluN2A which is likely to get phosphorylated in vitro at a putative site Ser1291. However, the regulation of phosphorylation of this site by kinases and phosphatases is not yet completely understood. In the present study, we have used the fusion constructs of GluN2A tagged with glutathione S-transferase (GST) as substrate for phosphorylation, purified calcium/calmodulin dependent protein kinase type II (CaMKII) and radioactive P32. We demonstrated that the site phosphorylated by αCaMKII on GluN2A was Ser1291 and that protein phosphatases 1, 2A and 2C were able to dephosphorylate this phospho-GST-GluN2A-Ser1291 in vitro. In the rat brain tissue post synaptic density and cytosolic fraction the major phosphatase responsible for dephosphorylating phospho-GluN2A-Ser1291 was protein phosphatase 1. Keywords: CaMKII, cytosol, GluN2A-Ser1291 subunite, N-methyl-D-aspartate receptors, protein phosphatase 1, rat brain","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the blood coagulation system in morbidly obese patients 病态肥胖患者凝血系统的特征

Ukrainian Biochemical Journal Pub Date : 2023-09-11 DOI: 10.15407/ubj95.04.003

D. S. Korolova, O. V. Hornytska, A. S. Lavrik, N. M. Druzhyna, N. Prysyazhna, T. M. Platonova

引用次数: 0

The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents 超重和肥胖青少年维生素D水平与心脏代谢紊乱之间的关系

Ukrainian Biochemical Journal Pub Date : 2021-05-20 DOI: 10.15407/UBJ93.02.072

H. Pavlyshyn, Anna-Mariia Shulhai

{"title":"The association between vitamin D status and cardiometabolic disorders in overweight and obese adolescents","authors":"H. Pavlyshyn, Anna-Mariia Shulhai","doi":"10.15407/UBJ93.02.072","DOIUrl":"https://doi.org/10.15407/UBJ93.02.072","url":null,"abstract":"An increase in the prevalence of overweight and obese adolescents is often combined with a low level of vitamin D that may be associated with lipid and carbohydrate metabolism disorders, which underlie the development of arterial hypertension and cardiometabolic changes. This work aimed to determine the association between vitamin D status and the main parameters of lipid and carbohydrate metabolism, and anthropometric measurements. A total of 196 adolescents (129 boys and 67 girls) from the Ternopil region were examined. Their mean age was 15.5 ± 2.3 years. Based on the body mass index (BMI): 60 adolescents had normal body weight, 60 were overweight, and 76 were obese. Anthropometric measurements and general examinations were performed. In blood serum 25(OH)D levels and the main parameters of lipid and carbohydrate metabolism were determined. Low levels of 25(OH)D were found. Prevalence of vitamin D deficiency among adolescents with BMI up to the 85th percentile was 56.7%, with BMI in the 85-97th percentiles was 70.0% and with BMI over the 97th percentile was 77.6%. Serum 25(OH)D levels in overweight and obese adolescents had a significant correlation with BMI (r = -0.427, P < 0.001), waist circumference (r = -0.462, P < 0.001), highdensity lipoprotein cholesterol (HDL-C, r = 0.214, P = 0.023), low-density lipoprotein cholesterol (LDL-C, r = -0.226, P = 0.011), atherogenic index (r = -0.284, P = 0.001), insulin level (r = -0.483, P < 0.001), and HOMA-IR index (r = -0.454, P < 0.001). We concluded that the development of cardiometabolic disorders in overweight and obese adolescents is associated with vitamin D status.","PeriodicalId":23448,"journal":{"name":"Ukrainian Biochemical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67022133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inflammation is the common mechanism of diseases (CMD) in COVID-19 disease during pregnancy and in gestational diabetes mellitus 炎症是妊娠期和妊娠期糖尿病新冠肺炎疾病（CMD）的常见发病机制

Ukrainian Biochemical Journal Pub Date : 2021-05-20 DOI: 10.15407/UBJ93.02.005

S. Vari

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The discovery of the mechanisms of biological synthesis of nucleic acids: 1959 Nobel laureates S. Ochoa and A. Kornberg 发现生物合成核酸的机制:1959年诺贝尔奖得主S. Ochoa和A. Kornberg

Ukrainian Biochemical Journal Pub Date : 2021-02-22 DOI: 10.15407/UBJ93.01.129

O. Matyshevska, V. M. Danilova, S. Komisarenko

引用次数: 0

Comparative characteristic of lung cancer stem-like cells generated in vitro under different culture conditions 不同培养条件下体外培养肺癌干细胞的比较特性

Ukrainian Biochemical Journal Pub Date : 2021-02-22 DOI: 10.15407/UBJ93.01.088

O. Skachkova, O. Gorbach, M. Inomistova, L. Garmanchuk, N. Khranovska

引用次数: 0